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Generate accurate Volcengine SDK examples by locating an API through API Explorer search, fetching its swagger, and calling api/common/explorer/make-code with user-provided Params. Use when the user asks how to write Volcengine SDK code, generate SDK samples, or call a Volcengine API in Python, Go, Java, PHP, cURL, Node.js. Supports Chinese and English API names such as "角色扮演", "AssumeRole", or "STS AssumeRole". Also use for explicit SDK configuration questions about retry, timeout, AK/SK, STS, AssumeRole, temporary credentials, proxy, connection pooling, SSL, debug mode, request signing, response parsing, and error handling. If the user only needs API parameters, enum values, required fields, error codes, response schemas, pagination, or API comparisons, hand off to volcengine-api. If they need CLI-based operations, hand off to volcengine-cli.

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Craft high-quality natural-language image prompts for any modern text-to-image or image-edit model that accepts flowing English. Trigger when the user wants help writing, rewriting, improving, or translating an English natural-language image prompt — including "write me an image prompt", "improve this image prompt", "describe this scene for an image model", or "convert these tags into a natural language prompt". Do NOT trigger for requests that are purely about dispatching to an image API, choosing samplers/schedulers, picking LoRAs, or setting up ControlNet — those belong to a runtime skill.

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Generate images via the Stable Diffusion WebUI / Forge HTTP API (AUTOMATIC1111-compatible `/sdapi/v1/*`). Use when the user wants to (1) discover or pick a model / extra module (TE/VAE) / sampler / scheduler / style preset from a running sd-webui server, (2) generate an image with a given prompt (txt2img), (3) check generation progress, (4) cancel/interrupt an in-flight generation, (5) inspect or change a global sd-webui option (e.g. active checkpoint), or (6) test connectivity. This skill talks to a *generic* sd-webui-compatible server (AUTOMATIC1111, Forge, reForge, sd-webui-forge-classic). Do NOT trigger for requests that are purely writing the prompt itself.

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Designing clinical trials — phases, randomization, endpoints, sample size, and CONSORT-compliant reporting.

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Audit laboratory automation systems -- LIMS architecture, instrument connectivity (SiLA 2, OPC-UA, serial drivers), sample tracking and chain of custody, protocol workflow engines, data acquisition pipelines, and regulatory compliance (21 CFR Part 11 electronic records/signatures.

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Design a research study. Use when the user says "study design", "design my research", "what research method should I use", "how do I test this hypothesis", "research plan", "methodology section", "experimental design", "survey design", "qualitative study design", "how to structure my study", "sample size", "data collection plan", or needs to plan how to test a research question or hypothesis - even if they don't explicitly say "study design".

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Generate A/B copy variants grounded in Eugene Schwartz's awareness and market-sophistication stages and John Caples' tested-headline discipline. Every variant declares its angle, the awareness stage it targets, and a falsifiable hypothesis, and the skill recommends which pair to test first and what delta counts as signal. Use when the user says "A/B test this", "write variants", "give me options", "test different angles", "copy variants", "ab copy", "test this headline", "multiple versions", "which version is better", or wants to test copy before publishing. For reviewing copy that already exists, see copy-review. For sample size and stopping rules, see growth-experiment. For diagnosing a page rather than its words, see page-cro. For full paid ad sets by platform, see ad-campaign-writer.

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Design statistically rigorous experiments to test data science hypotheses. Use when the user says "design an A/B test", "run an experiment", "test this hypothesis", "is this difference significant", "how many samples do I need", "randomized controlled trial", "causal inference", "uplift test", "holdout group", "significance test", "power calculation", "avoid p-hacking", or needs to determine whether an observed effect is real before making a product or model decision.

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Look up Microsoft API references, find working code samples, and verify SDK code is correct. Use when working with Azure SDKs, .NET libraries, or Microsoft APIs—to find the right method, check parameters, get working examples, or troubleshoot errors. Catches hallucinated methods, wrong signatures, and deprecated patterns by querying official docs.

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One page, hands-on manual tier — drive a live page through the web accessibility (a11y) checks a rule engine can't decide: keyboard operation and focus order, screen-reader names, roles and states from the accessibility tree, reflow and zoom, reduced motion, form errors, and target size. Grades each finding by evidence basis (verified / confirm-with-a-human / human-required) and severity, and closes every criterion in a ledger: verified, flagged, not exercised, or N/A. Locates and assesses; does not fix (use `accessibility-fix`). Use it for keyboard testing, focus-order checks, screen-reader or a11y-tree review, reflow and zoom at 200%, or 'is this operable, not just lint-clean'. The automated tier is `accessibility-scan`; `accessibility-audit` runs both across a sampled site.

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Whole site or product — a full web accessibility (a11y) audit against WCAG 2.2, following the WCAG-EM methodology. Defines scope, samples representative pages and flows, runs the automated tier (`accessibility-scan`) and the hands-on manual tier (`accessibility-inspect`), and produces one conformance report. Grades each finding by severity and evidence basis, and states per-criterion conformance as pass, fail, or undetermined (needs a human). Use it for 'audit my site for accessibility', 'is this product accessible', 'a11y audit', 'WCAG or Section 508 conformance report', or any multi-page assessment. Assesses; does not fix (use `accessibility-fix`) or diff (use `accessibility-diff`). For a single page use `accessibility-scan`; for hands-on keyboard and screen-reader checks use `accessibility-inspect`.

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Plan, prioritize, and design rigorous A/B tests using the Test Velocity Method. Use when a user wants to test a landing page, CTA, email, signup flow, pricing page, or any digital experience — this skill produces a complete, dev-ready test plan with hypothesis, sample size, duration, segmentation strategy, and guardrail metrics.

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Shape reusable library APIs for Compose modules. Design public composables, split sample-app code from reusable library code, review naming and extensibility.

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Build visual territories (mood boards) for team alignment before production. Groups references into 2-4 named territories, each with palette, texture, typography feel, and 4-6 generated sample tiles, so a team can agree on a direction. Use when the user says "moodboard", "visual territories", "align the team on a look", "reference board", or needs stakeholder sign-off on aesthetic before generating final assets. Part of the Creative Production set.

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Pick a visual direction before generating any creative. Produces 3-6 named, distinct visual directions (e.g. premium minimal, bold maximalist, lifestyle/editorial, retro, techno-brutalist) with rationale + sample prompts, then generates one reference image per direction so the user can choose. Use when the user says "explore visual direction", "what style should this be", "give me directions/looks", or before ads/scene/shot work when no style is locked. Part of the Creative Production set.

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Check and install prerequisites for the Todo app project. Use when setting up the development environment, when a user first opens the workspace, when troubleshooting missing tools, or when the user mentions setup, install, prerequisites, or environment.

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Bead-based signal normalization and cross-batch harmonization for CyTOF and high-parameter cytometry - EQ four-element bead normalization of instrument sensitivity drift (CATALYST normCytof, premessa), and reference-anchor cross-batch normalization (CytoNorm, per-cluster quantile splines). Covers the distinction between within-run drift correction and between-batch correction, the mandatory anchor/reference sample, why normalization is per-cluster with many quantiles, and the over-correction risk. Use when correcting CyTOF signal drift, harmonizing multi-batch or multi-site studies, or deciding whether to normalize data versus model batch in the design.

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Structures biological experiments so inference is valid by construction, covering Fisher's principles (randomization, replication, local control), the experimental-vs-observational unit distinction and pseudoreplication (Hurlbert 1984; Lazic 2018), randomization mechanics (complete, restricted, stratified, rerandomization, run-order), blocking layouts (randomized complete block, Latin square, incomplete block), factorial designs and interactions, and the split-plot/nested error strata hidden inside multi-batch genomics. Use when deciding the experimental unit and what counts as a replicate, planning randomization and run order, choosing a blocked/factorial/split-plot/nested layout, avoiding pseudoreplication in cell-culture or animal studies, or specifying the random-effects structure of the analysis model. For assigning samples to sequencing batches/lanes/plates and batch-effect correction see experimental-design/batch-design; for regulated clinical-trial randomization see clinical-biostatistics.

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Defines cell populations in flow and spectral cytometry through manual gates (rectangle, polygon, quadrant, boolean) and reproducible automated gating (openCyto gating templates, flowDensity data-driven thresholds, flowClust model-based gates), organized as a hierarchical GatingSet (flowWorkspace) and round-tripped with FlowJo via CytoML. Covers the canonical gate order (time -> debris -> singlets -> live -> lineage), FMO-vs-isotype boundary setting, gate-order dependence and recompute semantics, rare-event/MRD gating, and per-population statistics. Use when building a gating strategy, automating a manual FlowJo scheme across samples, choosing manual vs data-driven gates, or extracting population frequencies.

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Quality control for flow, spectral, and mass cytometry - time-based anomaly cleaning (flowAI, flowCut, PeacoQC, flowClean), margin/boundary event removal, signal-drift detection, dead-cell exclusion, CyTOF Gaussian/DNA/event-length checks, instrument calibration/standardization (MESF, CS&T, peak-2), and batch-level outlier flagging. Use when assessing acquisition quality, choosing a cleaning tool, ordering QC relative to compensation, deciding margin removal before density-based steps, or flagging problematic samples before clustering or differential analysis.

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Imports gene expression count matrices from featureCounts, HTSeq, STAR ReadsPerGene, Salmon/kallisto via tximport or tximeta, RSEM, 10X Genomics MTX/H5, AnnData H5AD, and RDS. Handles silent-miscounting traps (featureCounts -p v2.0.2 API break, STAR strandedness column choice, salmon NumReads-sum without tximport, RSEM non-integer expected_count, GENCODE _PAR_Y suffix, zero-length-transcript TPM divide-by-zero), and encodes the tximport countsFromAbundance decision tree with the "lengthScaledTPM is not TPM" warning. Use when assembling a gene-by-sample count matrix from aligner or quantifier output, importing salmon/kallisto for DESeq2 vs limma-voom, choosing strandedness column for STAR, debugging zero-count panics, or building tx2gene mapping.

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Differential abundance (DA) and differential state (DS) analysis for flow and mass cytometry - tests which cell populations change in frequency or marker expression between conditions using diffcyt (edgeR/voom/GLMM for DA, limma/LMM for DS), with cydar, CITRUS, and compositional methods (sccomp, scCODA, DCATS) as alternatives. Covers the sample-is-the-experimental-unit principle, design/contrast and mixed-model formulas, compositionality of cluster proportions, and FDR across clusters. Use when comparing populations between groups, choosing a DA method, handling paired/batch designs, or deciding whether compositional correction is needed.

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Infers person-to-person transmission from pathogen genomes using outbreaker2, TransPhylo, phybreak, BadTrIP, SCOTTI, BEASTLIER, and SNP-distance / cluster-picker approaches (HIV-TRACE for HIV; transcluster). Defines outbreak clusters using pathogen-specific SNP thresholds (NOT a universal cutoff -- TB <=12 SNPs; MRSA <=15; C. difficile <=2; Klebsiella <=21), models within-host diversity and transmission bottlenecks, integrates contact-tracing data, distinguishes generation from serial interval, and attributes source via Bayesian source attribution (islandR). Use when investigating outbreaks for who-infected-whom, defining SNP-cluster outbreak definitions, accounting for unsampled intermediates, choosing between outbreaker2 (rich epi data) and TransPhylo (genomic-only after a dated phylogeny), running source attribution between host populations, calling HIV-TRACE thresholds appropriate to the local subtype, or distinguishing recent transmission from reactivation in TB or chronic HIV.

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Creates DE-specific diagnostic and result visualizations using DESeq2/edgeR built-in functions and lightweight ggplot2 wrappers. Covers MA plot (with the shrunken-LFC compression effect), volcano (with the apeglm caveat that p-values are unchanged), PCA on VST/rlog (never raw counts), sample distance heatmaps, top-DE-gene heatmaps with the row-scaling trap, dispersion / BCV plot interpretation, p-value histogram diagnostics, plotCounts for individual genes, blind=TRUE vs FALSE rationale, and the n=3 visualization stake. Use when generating DE diagnostic plots, choosing VST vs rlog for visualization, troubleshooting suspicious plot patterns (shifted MA cloud, batch-dominated PCA, anti-conservative p-value histogram), or building a standard QC figure panel.

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