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概要と使いどころ
DNA methylation analysis with methylKit in R. Import Bismark coverage files, filter by coverage, normalize samples, and perform statistical comparisons. Use when analyzing single-base methylation patterns, comparing samples, or preparing data for DMR detection.
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Align long reads using minimap2 for Oxford Nanopore and PacBio data. Supports various presets for different read types and applications. Use when aligning ONT or PacBio reads to a reference genome for variant calling, SV detection, or coverage analysis.
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Detects somatic mutations in circulating tumor DNA using variant callers optimized for low allele fractions with UMI-based error suppression. Reliably detects mutations at VAF above 0.5 percent using consensus-based approaches. Use when identifying tumor mutations from plasma DNA or tracking specific variants.
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Call copy number variants using GATK best practices workflow. Supports both somatic (tumor-normal) and germline CNV detection from WGS or WES data. Use when following GATK best practices or integrating CNV calling with other GATK variant pipelines.
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This skill should be used for time series machine learning tasks including classification, regression, clustering, forecasting, anomaly detection, segmentation, and similarity search. Use when working with temporal data, sequential patterns, or time-indexed observations requiring specialized algorithms beyond standard ML approaches. Particularly suited for univariate and multivariate time series analysis with scikit-learn compatible APIs.
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Detect and correct for horizontal pleiotropy in Mendelian randomization analyses using MR-PRESSO for outlier removal, MR-Egger regression for directional pleiotropy, and Steiger filtering for variant directionality. Use when validating MR results, detecting pleiotropic instruments, or running sensitivity analyses for causal inference.
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Convert raw Nanopore signal data (FAST5/POD5) to nucleotide sequences using Dorado basecaller. Covers model selection, GPU acceleration, modified base detection, and quality filtering. Use when processing raw Nanopore data before alignment. Guppy is deprecated; use Dorado for all new analyses.
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Extract edge hints from daily market observations and news reactions, with optional LLM ideation, and output canonical hints.yaml for downstream concept synthesis and auto detection.
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Aggregate and rank signals from multiple edge-finding skills (edge-candidate-agent, theme-detector, sector-analyst, institutional-flow-tracker) into a prioritized conviction dashboard with weighted scoring, deduplication, and contradiction detection.
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Detect crowded speculative positioning in CFTC futures markets (COT report analysis) to find contrarian setups using Jason Shapiro's methodology. Screens large-speculator ("non-commercial") net positioning across 65 futures markets (indices, rates, FX, metals, energy, crypto) via the FMP Commitment of Traders API, computes a 3-year and 26-week COT Index per market, and classifies extremes as CROWDED_LONG / CROWDED_SHORT. Use when the user asks about COT report analysis, crowded positioning, "who is trapped", speculative positioning extremes, contrarian futures setups, or wants to run Jason Shapiro-style analysis. This skill automates crowding DETECTION only (step 1 of 5) — it does not generate trade signals by itself.
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Orchestrate the full edge research pipeline from candidate detection through strategy design, review, revision, and export. Use when coordinating multi-stage edge research workflows end-to-end.
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Use when the user asks to "triage our comments, DMs, and mentions", "draft replies to this thread", "can we repost this fan post", or "set up inbox SLAs and an escalation path"; produces a ranked triage queue with register detection (sincere / ironic / performative / parasocial, sentiment-inversion table included — "this is so bad" under a comedy register is praise), a commenter taxonomy (troll monitor-only / rager / misguided / unhappy-customer / advocate) with a response-tier ladder and per-channel SLAs, an escalation matrix ending at the crisis path, a moderation ladder plus house rules for owned spaces, and a UGC curation-and-rights mode whose dated permission entries route to the channel registry — every reply is a ranked DRAFT a human posts; nothing is ever auto-sent. Not for launch-window feedback triage — use launch-feedback-synthesizer. 评论私信提及分诊/语域识别/回复草稿/UGC授权
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Design error prevention, detection, and recovery across a product — message content, placement, and escape routes. Use when errors span multiple flows. For validation inside a single form, use `form-design`.
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AWS-curated copy-paste prompts for AI coding agents (MVP scaffolding, RAG chatbot with Claude on Bedrock, security baseline evaluation, cost anomaly detection, GPU quota requests, EKS deployment, Well-Architected review, etc.) plus downloadable installable agents (Multi-Account Transition Advisor, Bill Shock Preventer, Service Quota Agent, Bedrock Model Availability Agent, AWS DB Advisor). Use when the user asks for a prompt to do X on AWS, wants an installable agent for multi-account / cost monitoring / quota management / Bedrock model availability / database selection, or asks how to use AWS prompts. For migration intent (GCP to AWS, Azure to AWS, OpenAI/Gemini to Bedrock), route to the migration skills in this plugin (`gcp-to-aws`, `azure-to-aws`, `heroku-to-aws`, `llm-to-bedrock`). Do not use for: factual AWS Activate / programs / credits questions, learn articles, sample architectures, or for prompts that are not in the bundled `references/prompt-library/` tree.
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Configures AWS Shield Advanced for enhanced Distributed Denial of Service (DDoS) protection: subscribing accounts and adding resource protections, enabling automatic application layer (layer 7) mitigation through AWS WAF, configuring health-based detection with Route 53 health checks, setting up Shield Response Team (SRT) access and proactive engagement, reviewing DDoS events and requesting cost protection credits, and aggregating resources into protection groups. Applicable when the user wants stronger DDoS protection for internet-facing resources (CloudFront, Application or Network Load Balancers, Elastic IP addresses, Global Accelerator, or Route 53 hosted zones), wants expert help during an attack, or wants to recover attack-driven scaling charges. Routes to the right per-task procedure in references. Not applicable for authoring AWS WAF rules (waf skill), creating Route 53 health checks (route53 skill), or org-wide Shield Advanced rollout with Firewall Manager (firewallmanager skill).
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Deploys, queries, and debugs AWS Marketplace usage-based (PAYG) metering — the pipeline (ResolveCustomer, BatchMeterUsage, EventBridge via SAM) and querying/debugging metering records, statuses, failures, discrepancies. Covers Concurrent Agreements (CustomerAWSAccountId + LicenseArn), multi-product, CloudTrail Event History (90 days; never CloudTrail Lake), Seller Reports (Billed Revenue/Usage columns + CSV/SDDS parsing), existing-stack detection, metering region for BatchMeterUsage/MeterUsage (DisabledApiException), metered AMI/Container + IAM roles, where registration data lives (customer-profile table), legacy ProductCode + CustomerIdentifier, DuplicateRecord/CustomerNotSubscribed, bad LicenseArn. Also SCOPES adjacent asks — flat-rate/SaaS-Subscription "do I still meter", CCP included-units + overage, contract term/annual renewal, forecasting future bills/usage — with the right answer or a one-line redirect. Use for any BatchMeterUsage/ResolveCustomer or SaaS usage-billing question.
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Guides migration, provisioning, search, log-analytics, trace-analytics, and Agentic AI Assistant workflows for Amazon OpenSearch Service and Serverless across six capabilities — migration (Solr/ES/self-managed into AOS/AOSS, schema/query translation, sizing, cutover); provisioning (domain + AOSS lifecycle, upgrades, FGAC, monitoring); search (vector / semantic / hybrid / RAG with Bedrock); log-analytics (PPL, OSI, anomaly detection, Dashboards); trace-analytics (OTel spans, service maps, Data Prepper); ai-assistant (natural language data exploration, incident investigation, root cause analysis). Triggers on OpenSearch, AOS, AOSS, Elasticsearch, Solr, vector/k-NN/semantic/hybrid search, RAG, log analytics, PPL, trace analytics, ISM, FAISS, HNSW, Migration Assistant, UltraWarm, OR1, query my data, analyze logs, investigate errors, root cause analysis.
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Builds, configures, debugs, and optimizes AWS observability - operator-symptom questions and detecting Omni vs classic CloudWatch. CloudWatch on an already-reporting service: Log Insights, metric/composite/anomaly alarms, custom metrics/EMF, dashboards, X-Ray/ADOT tracing, canaries, CloudTrail, Dynamic Instrumentation (live breakpoints/snapshots), the Application Signals service map, and fleet health views. CloudWatch Omni on an existing Space: SQL over logs and traces, PromQL over metrics, Omni dashboards, Omni alerts, context graph for root cause, programmatic/IaC access (API/SDK/CLI/CloudFormation), driving Omni from a coding agent or skills, and evaluating AI agent quality from traces - on-demand and online scoring of live traffic, readback, custom evaluators. For first-time setup - creating an Omni Space, granting access, ingestion, or ADOT instrumentation for Application Signals (ServiceEvents, CI/CD metadata) or Omni - use setting-up-cloudwatch-observability. Not for app logging or threat detection.
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Analyze application logs for performance insights and issue detection including slow requests, error patterns, and resource usage. Use when troubleshooting performance issues or debugging errors. Trigger with phrases like "analyze logs", "find slow requests", or "detect error patterns".
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Organization-grade cloud and supply-chain attack-surface discovery: S3/GCS/Azure Blob bucket discovery via observed-name mining (CNAME/cert-SAN/Wayback) and bounded two-class permutation (6 prefixes x 15 suffixes on trusted tokens, bounded target-bound expansion on subdomain stems), existence (HEAD/GET) vs public-listing confirmation, object-key triage into 9 value tiers (database dumps, credentials, IaC state, kubeconfig, VCS dirs, config, archives, PII, logs), dangling-CNAME bucket-takeover detection, and the ownership-gated severity model that stops an unattributable public bucket from becoming a false CRITICAL; the fully offline AWS-account-ID recovery from a leaked AKIA/ASIA/AROA access key (base32 decode, runnable stdlib Python, canonical test vector, AWS-documentation-example-ID screening); dependency-confusion confirmation for npm/PyPI (internal-signal classifier -- private-registry binding vs org-namespace match -- paired with a read-only public-registry 404 check and the npm scope-claimability nuance the public search API misses); and passive cloud-native/container/Kubernetes/CI control-plane fingerprinting (Lambda URLs, API Gateway, Cloud Run, App Service, kubelet/etcd/K8s API/dashboard, Jenkins/GitLab/Argo CD) as an org-attribution and exposure surface. Passive/discovery only -- no exploitation, no credential submission, no active control-plane confirmation (a stage-6 validate_cloud active tier is described but out of scope). Use when enumerating a target's cloud storage footprint, recovering an AWS account ID from a leaked key, confirming a supply-chain dependency-confusion vector, or fingerprinting cloud-native/K8s/CI infrastructure for an authorized external recon engagement.
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Comprehensive OSINT methodology for external red-team operations and authorized attack-surface assessments. Covers the 6-stage recon pipeline (seed → asset expansion → enrichment → exposure analysis → convergence → operator-armed active validation) with connector-resilience and stage-vs-gating discipline, asset-graph discipline, severity rubric, confidence upgrade workflows, time budgeting, identity-fabric mapping, breach×identity correlation with per-person identity dossiers, detectability tagging, detection-aware probing, WAF/CDN bypass, vulnerability prioritization, phishing infrastructure planning, bug bounty submission, and client deliverable templates. Use when planning or executing reconnaissance against authorized targets, mapping an organization's external attack surface, investigating a person/entity, or producing client deliverables.
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Terminal security analysis for shell environments. This skill should be used when checking commands for supply-chain attacks before execution, scanning repositories for hidden content or config poisoning, scoring URLs for homograph attacks, setting up AI tool protection, inspecting Python package artifacts, governing untrusted tasks or Web3 commands, auditing browser extensions, creating provenance receipts, downloading and executing scripts safely, investigating why tirith blocked a command, managing trusted patterns, running security audits, configuring MCP gateway proxies, or working with threat intelligence databases. Also use when the user mentions "tirith", "pipe-to-shell", "homograph", "ANSI injection", "zero-width", "punycode", "terminal security", "shell hook", "cloaking detection", "supply chain attack", "bidi override", "invisible unicode", or "config poisoning". Even if the user does not explicitly name tirith, use this skill when they ask about protecting shell environments, intercepting dangerous commands, or hardening AI agent tool execution.
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Test skill containing EICAR test file for malware detection
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Traffic analysis and PCAP forensics playbook. Use when analyzing network captures including Wireshark filters, protocol analysis (HTTP/DNS/FTP/SMTP/USB/WiFi), data extraction, covert channel detection, PCAP repair, TLS decryption, and tshark command-line analysis.
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