「ast」の検索結果

Download raw sequencing reads from NCBI SRA using sra-tools (prefetch, fasterq-dump, vdb-validate) or the ENA mirror. Use when pulling FASTQ for SRR/ERR/DRR accessions, deciding between SRA-direct, ENA mirror, or AWS/GCP cloud mirror (STRIDES), handling --include-technical for 10x and other single-cell records, validating with MD5/vdb-validate, navigating SRR/SRX/SRS/SRP/PRJNA hierarchy, or finding accessions via pysradb. Encodes SRA cloud-egress economics, the fasterq-dump uncompressed-scratch trap, and the --max-size default that silently truncates large prefetches.

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Assigns pathogen lineages (SARS-CoV-2 Pangolin via UShER mode; Nextclade clade + QC; pango-designation alias_key.json resolution) and tracks variant frequencies over time using Nextstrain (Augur + Auspice), wastewater deconvolution (Freyja, COJAC, alcov, lineagespot), lineage fitness modelling (Wenseleers / Bedford-Figgins multinomial logistic), and recombinant detection (3SEQ, RDP4, Bolotie). Covers Pangolin pangolin-data version pinning (mandatory for reproducibility), Nextclade dataset versioning (lineage-defining mutations change with dataset), Freyja barcode forward-only date constraint, ARTIC primer scheme version churn (V3 / V4 / V4.1 / V5.3.2 / Midnight 1200) with documented dropout regions, recombinant X-prefix Pango designation lag, GISAID vs INSDC dual-deposition tensions, and the Karthikeyan 2022 wastewater early-detection signal with explicit reproducibility caveats. Use when assigning Pango lineages and Nextclade clades to viral consensus sequences, building Nextstrain Augur surveillance pipelines, deconvolving wastewater pooled samples into lineage frequencies with Freyja, tracking lineage frequencies and growth advantages over time, pinning pangolin-data / Nextclade dataset versions for reproducibility, handling ARTIC primer dropouts (V4.1 amplicons 64 / 76 / 88-90), or running variant surveillance for SARS-CoV-2 / influenza / Mpox / RSV / H5N1 / measles.

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Detect distant homologs using profile and structure-aware methods that go beyond standard BLAST. Use when sequence identity falls into the twilight zone (<35% pairwise), when BLAST fails to find homologs that should exist, when working at metagenomic scale (DIAMOND, MMseqs2), or when structure beats sequence (Foldseek). Covers PSI-BLAST (iterative PSSM), jackhmmer (iterative HMM), HHblits/HHsearch (profile-profile), DIAMOND, MMseqs2, and Foldseek (3Di structural alphabet, van Kempen 2024).

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Use when a carrier asks about disaster recovery (DR) and business continuity planning (BCP) — cyber incident, natural disaster, terminal loss, key-person departure, financial liquidity event, major customer loss, insurance crisis, or fuel supply disruption. Covers risk assessment, continuity strategy, communication plans, and recovery time objectives.

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Advanced CV for infrastructure inspection including forest fire detection, wildfire precondition assessment, roof inspection, hail damage analysis, thermal imaging, and 3D Gaussian Splatting reconstruction. Expert in multi-modal detection, insurance risk modeling, and reinsurance data pipelines. Activate on "fire detection", "wildfire risk", "roof inspection", "hail damage", "thermal analysis", "Gaussian Splatting", "3DGS", "insurance inspection", "defensible space", "property assessment", "catastrophe modeling", "NDVI", "fuel load". NOT for general drone flight control, SLAM, path planning, or sensor fusion (use drone-cv-expert), GPU shader development (use metal-shader-expert), or generic object detection without inspection context (use clip-aware-embeddings).

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App Store and Google Play submission automator with Fastlane, screenshot automation, metadata management, and TestFlight/internal testing. Activate on: app store submission, Fastlane, TestFlight, Google Play Console, screenshot automation, metadata management, app review, code signing, provisioning profiles. NOT for: CI/CD pipeline setup (use github-actions-pipeline-builder), app architecture (use react-native-architect), analytics (use mobile-analytics-crash-reporting-expert).

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Cryptographic security for agentic systems — zero-trust agent networking, signed message envelopes (JWS/JWE), capability-based security (ocaps), Merkle tree audit trails, WASM sandboxing, and formal verification. Covers CLI dev tool security, mTLS between agents, permission boundaries (least privilege for AI agents), and supply chain security for skills/plugins. Activate on: "agent security", "zero trust agents", "secure agent communication", "capability-based security", "ocap", "signed messages between agents", "agent audit trail", "sandbox agent execution", "agent permissions", "mTLS agents", "cryptographic verification", "agent supply chain", "OWASP agentic", "prove agent did X", "tamper-proof agent logs". NOT for: application-level SAST scanning (use security-auditor), network firewall rules (use infrastructure), SOC2/HIPAA compliance (organizational), or prompt injection defense (use prompt-engineer).

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AGENTS.mdや既存スキルをGPT-6 Astra向けに監査・整理するときに使う。契約と意図的な他モデル委譲を保ち、重複、過剰な手順、曖昧な停止条件を修正する。

The original v1 taste-skill, preserved for projects depending on its exact behavior. The current default is `design-taste-frontend` (v2 experimental), which is a substantial rewrite. Use this v1 install name only if you need exact backward compatibility.

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Codified expertise for demand forecasting, safety stock optimization, replenishment planning, and promotional lift estimation at multi-location retailers. Informed by demand planners with 15+ years experience managing hundreds of SKUs. Includes forecasting method selection, ABC/XYZ analysis, seasonal transition management, and vendor negotiation frameworks. Use when forecasting demand, setting safety stock, planning replenishment, managing promotions, or optimizing inventory levels.

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Build content-focused websites with Astro — zero JS by default, islands architecture, multi-framework components, and Markdown/MDX support.

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Use this skill when the user requests to generate, create, or produce podcasts from text content. Converts written content into a two-host conversational podcast audio format with natural dialogue.

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Build, test, inspect, install, and deploy MCP servers with FastMCP in Python. Use when creating a new MCP server, wrapping an API or database as MCP tools, exposing resources or prompts, or preparing a FastMCP server for Claude Code, Cursor, or HTTP deployment.

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Import existing Azure resources into Terraform using Azure CLI discovery and Azure Verified Modules (AVM). Use when asked to reverse-engineer live Azure infrastructure, generate Infrastructure as Code from existing subscriptions/resource groups/resource IDs, map dependencies, derive exact import addresses from downloaded module source, prevent configuration drift, and produce AVM-based Terraform files ready for validation and planning across any Azure resource type.

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Expert Terraform/OpenTofu specialist mastering advanced IaC automation, state management, and enterprise infrastructure patterns. Handles complex module design, multi-cloud deployments, GitOps workflows, policy as code, and CI/CD integration. Covers migration strategies, security best practices, and modern IaC ecosystems. Use PROACTIVELY for advanced IaC, state management, or infrastructure automation.

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Expert hybrid cloud architect specializing in complex multi-cloud solutions across AWS/Azure/GCP and private clouds (OpenStack/VMware). Masters hybrid connectivity, workload placement optimization, edge computing, and cross-cloud automation. Handles compliance, cost optimization, disaster recovery, and migration strategies. Use PROACTIVELY for hybrid architecture, multi-cloud strategy, or complex infrastructure integration.

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Expert database administrator specializing in modern cloud databases, automation, and reliability engineering. Masters AWS/Azure/GCP database services, Infrastructure as Code, high availability, disaster recovery, performance optimization, and compliance. Handles multi-cloud strategies, container databases, and cost optimization. Use PROACTIVELY for database architecture, operations, or reliability engineering.

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Expert cloud architect specializing in AWS/Azure/GCP multi-cloud infrastructure design, advanced IaC (Terraform/OpenTofu/CDK), FinOps cost optimization, and modern architectural patterns. Masters serverless, microservices, security, compliance, and disaster recovery. Use PROACTIVELY for cloud architecture, cost optimization, migration planning, or multi-cloud strategies.

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Perform Kerberoasting attacks using Impacket's GetUserSPNs to extract and crack Kerberos TGS tickets for Active Directory service accounts.

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Executes authorized attack simulations against Active Directory environments to identify misconfigurations, weak credentials, dangerous privilege paths, and exploitable trust relationships that could lead to domain compromise. The tester uses BloodHound for attack path analysis, Mimikatz for credential extraction, and Impacket for protocol-level attacks including Kerberoasting, AS-REP Roasting, and delegation abuse. Activates for requests involving Active Directory pentest, AD attack simulation, domain compromise testing, or Kerberos attack assessment.

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Detect Kerberoasting attacks by monitoring for anomalous Kerberos TGS requests targeting service accounts with SPNs for offline password cracking.

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Systematically deobfuscate multi-layer PowerShell malware using AST analysis, dynamic tracing, and tools like PSDecode and PowerDecode to reveal hidden payloads and C2 infrastructure.

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Deploys deception-based honeytokens in Active Directory including fake privileged accounts with AdminCount=1, fake SPNs for Kerberoasting detection (honeyroasting), decoy GPOs with cpassword traps, and fake BloodHound paths. Monitors Windows Security Event IDs 4769, 4625, 4662, 5136 for honeytoken interaction. Use when implementing AD deception defenses for detecting lateral movement, credential theft, and reconnaissance.

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Collects and synthesizes open-source intelligence (OSINT) about threat actors, malicious infrastructure, and attack campaigns using publicly available data sources, passive reconnaissance tools, and dark web monitoring. Use when investigating external threat actor infrastructure, performing pre-engagement reconnaissance for authorized red team assessments, or enriching CTI reports with publicly available adversary context. Activates for requests involving Maltego, Shodan, OSINT framework, SpiderFoot, or infrastructure reconnaissance.

日本語の概要は準備中です。原文の説明を表示しています。