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Processes eDNA metabarcoding from raw paired-end reads to species tables, navigating ASV (DADA2, UNOISE3) vs OTU (swarm v2) decision (Callahan 2017 vs Schloss multi-copy-16S critique), marker/primer choice (Leray COI, MiFish 12S, 515F/806R 16S, ITS2) with primer-specific bias, OBITools3 v3 command-name break (obi stats plural; .tar.gz taxonomy), tag-jumping with dual-indexing (Schnell 2015; NovaSeq 10x MiSeq), decontam as screening-not-classifier (Davis 2018), read-counts-not-abundance critique (Lamb 2019), site-occupancy modeling (Ficetola 2015), Naive-Bayes calibration limits (Bokulich 2018), and eDNA decay (Strickler 2015). Use when going from raw eDNA FASTQ to species tables, picking marker + denoising pipeline, deciding whether read counts represent abundance, applying occupancy modeling, configuring OBITools3 v3, or interpreting decontam output. Not for clinical 16S microbiome (see microbiome/amplicon-processing).

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Assesses the quality and completeness of a genome annotation with BUSCO (conserved single-copy ortholog recovery), OMArk (proteome completeness, consistency, and contamination), CheckM2 (prokaryotic completeness/contamination), and a gene-set sanity panel (gene count, mono-exonic fraction, protein-length distribution, mRNA:gene ratio, coding density). Covers the assembly-BUSCO-vs-proteome-BUSCO diagnostic, what BUSCO-Duplicated really means, why gene count is a vanity metric, and the QC of transferred annotations. Use when judging whether an annotation is good enough to publish or submit, diagnosing a suspect annotation, or comparing annotation completeness across pipelines.

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Quality control for pooled CRISPR screens covering library representation, Gini index, log-skew, replicate Pearson and Spearman concordance, essentialome precision-recall AUC against CEGv2 (Hart 2017), Cas9 cut-toxicity diagnostics, copy-number amplicon detection (Aguirre 2016 / Munoz 2016), bottleneck propagation through plasmid pool, infection, selection, and endpoint stages, MOI verification, and DepMap-style screen-quality scoring. Use when assessing screen quality before hit calling, deciding whether to repeat or rescue a screen, diagnosing low-confidence hits, choosing between MAGeCK / BAGEL2 / Chronos based on quality grade, picking a normalization strategy from QC signatures, or evaluating whether an in-vivo screen retained adequate library complexity.

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Infer orthologous genes and gene families across species using OrthoFinder3 (HOG-based phylogenetic orthology), SonicParanoid2, Broccoli, ProteinOrtho, OMA / FastOMA hierarchical orthologous groups, eggNOG-mapper, JustOrthologs, and TOGA whole-genome-alignment orthology. Use when building single-copy ortholog sets for phylogenomics, classifying co-orthologs and in/out-paralogs after gene duplication, propagating functional annotation via orthology with awareness of the ortholog conjecture, distinguishing speciation from duplication via gene-tree species-tree reconciliation, computing Quest-for-Orthologs benchmark performance, or running synteny-aware ortholog detection in WGD-affected lineages.

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Use when the question is about Swift or its toolchain rather than a framework — modern idioms, noncopyable types, drag and drop, debug deep links, tvOS targets.

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This skill should be used when creating Ultralytics-branded content of any kind, including PDF, PPTX, Canva, or Google Slides presentations, DOCX documents, marketing or newsletter HTML emails, social media post copy or visuals, website or landing page designs, and dataset or model result visuals. Provides official colors, typography, logo rules, naming rules, and tone of voice from ultralytics.com/brand.

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This skill should be used when the user asks to "review writing", "check documentation style", "audit interface copy", or "apply writing guidelines".

日本語の概要は準備中です。原文の説明を表示しています。

Patterns for using blockedImage to process large images, harness parallel compute for image processing, and write custom adapters. Use when writing code that creates, processes, or visualizes blockedImage objects, when implementing images.blocked.Adapter subclasses, or when a user needs help with large image data. Always use this skill when working with TIFF,GeoTIFF, .svs, .ndpi, .czi or other WSI, satellite imagery or microscopy volume image formats.

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Load this first for any task involving images, pictures, photos, scans, frames, volumes, or visual data — including reading, writing, filtering, enhancing, denoising, sharpening, deblurring, thresholding, segmenting, masking, detecting, finding or counting objects, removing backgrounds, measuring, comparing, registering, stitching, aligning, cropping, resizing, transforming, or analyzing. Use for image I/O (TIFF, PNG, JPEG, RAW, HDR), histograms, edge detection, color processing, morphology, geometric transforms, deep learning for images, quality assessment, and 3-D volume processing. Also triggers when users describe image problems (dark, noisy, blurry, low contrast, uneven lighting, hazy, color cast) or data exploration tasks (folder of images, characterize data, understand format). Handles microscopy (cells, particles) and general scientific imaging. Routes to sibling skills for medical imaging (DICOM, NIfTI), hyperspectral data, and visual inspection.

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AWS EC2 virtual machine management — instances, security groups, key pairs, AMIs, EBS volumes, Auto Scaling Groups, Spot Instances, Session Manager, placement groups, and instance lifecycle automation. Trigger on ANY of these, even when EC2 isn't named explicitly: - Launching or provisioning: "spin up a server", "create a VM", "new instance", "run-instances", mention of instance types (t3, m5, c5, r6, g5, p4d, t4g, c7g, etc.) - SSH / connectivity problems: "connection refused", "connection timed out", "permission denied publickey", "can't connect to my instance", "SSH not working" - Instance management: resize, stop, start, terminate, reboot, change instance type - Cost optimization: stop dev instances overnight, save money on EC2, spot vs on-demand, reserved instances - Auto Scaling: ASG, launch template, mixed instances policy, scale to zero, scheduled scaling - Spot Instances: spot fleet, spot interruption, capacity-optimized, price-capacity-optimized - AMIs and backups: create image, custom AMI, EBS snapshot, DLM lifecycle policy, copy AMI - Monitoring: EC2 CPU utilization, CloudWatch metrics for instance, instance status checks, console output - Access methods: Session Manager, EC2 Instance Connect, bastion host, port forwarding - Security: IMDSv2, instance metadata, IAM role on instance, security group rules - User data and bootstrap scripts, cloud-init

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E-commerce product description generator for any platform. Generates optimized titles, bullet points, descriptions, and backend keywords using competitor research + keyword scoring + FABE copywriting. Two modes: (A) Create — generate listing from product specs with optional competitor analysis, (B) Optimize — improve existing listing with keyword gap analysis. Supports Amazon, eBay, Walmart, Shopify, Etsy, TikTok Shop, Lazada, Shopee. No API key required. Use when: (1) writing a new product listing, (2) analyzing what makes competitors rank, (3) improving an underperforming listing.

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Audit and optimize e-commerce landing pages for conversion. CTA placement, trust signals, page structure, copy optimization, and A/B testing strategy for product pages, collection pages, and campaign landing pages.

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Product description writing — keyword integration, benefit-focused copy, FAQ, formatting

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Cross-platform PPC strategy planner for ecommerce businesses. Analyzes your product and margins, recommends the right advertising platforms (Google Ads, Meta Ads, TikTok Ads), calculates ROAS targets, allocates budget across channels, and generates platform-specific campaign briefs with ad copy and creative direction. Two modes: (A) Build — design a multi-platform ad strategy from scratch, (B) Optimize — audit existing cross-platform campaigns and reallocate budget. Works for Shopify, WooCommerce, standalone stores, and marketplace sellers expanding to external traffic. No API key required.

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Analyze, standardize, validate, and sync locally maintained skills into agent skill directories with a `skills` CLI-aligned workflow. Use this skill when Codex needs to turn ad-hoc prompt or rules folders into reusable `SKILL.md`-based skills, install or sync one or more local skills from `./skills` into Claude, Cursor, CodeBuddy, Codex, or similar agent directories, or manage local skill path mappings and symlink or copy installation behavior.

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Author or revise a cloud-api-operations recipe (config/source/skills/cloud-api-operations/references/recipes/). Encodes why the long tail of control-plane capabilities is deliberately NOT wrapped as MCP/CLI tools, the API-first admission test for whether a capability deserves a recipe, the negative and positive lists, the fixed recipe structure, the writing rules distilled from writing-for-agents, the user-facing copy red lines, and the product regeneration/verification pipeline. Also routes contributors (and their agents) to the contribution flow and the pre-PR self-test protocol in references/contributing.md. Use when adding a recipe, judging whether a scenario belongs in the recipe set, updating the recipes index, re-checking existing recipes against the admission test, or onboarding someone who wants to submit one.

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Paid advertising automation for Google Ads, Meta Ads, and other ad platforms. Creates campaigns, optimizes budgets, manages keywords, writes ad copy, and tracks ROAS. Leverages third-party ad management skills when available. Use when the user needs ad campaigns, PPC, SEM, paid traffic, or says 'run ads', 'Google Ads', 'Meta Ads', 'Facebook Ads', 'ad campaign', 'PPC', 'ROAS', or 'paid traffic'.

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MANDATORY for every coding agent and contributor touching localized content — keep all five localization surfaces (dashboard UI keys, wiki page, full mirrored guides, locale-aware formatting, language switchers) in parity across every supported language. Use automatically (without being asked) whenever you add or change user-visible UI copy, add an i18n key, edit README-EN.md or a localized guide, edit wiki/index.html, or add a language surface.

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MANDATORY for every coding agent (Claude Code, Codex, or any other) on every change-set — every applicable source file the agent creates or updates MUST start with the project's copyright/authorship header (file overview + exact author line). Use automatically whenever writing a new file or editing an existing one; do not wait to be asked. Covers JS/TS/TSX/CJS/MJS, Python, shell, and CSS. Includes the audit script to verify repo-wide compliance.

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American business English writing style for professional communications — direct, action-oriented, EN-US spelling (color, organize, center). Use whenever the user is writing for an American audience: emails, chat messages, proposals, client communications, blog posts, web copy, or any business writing. Apply to drafting, editing, and tone-checking professional text.

日本語の概要は準備中です。原文の説明を表示しています。

New Zealand business English writing style for professional communications — warm, inclusive, EN-NZ spelling (colour, organise, centre). Use whenever the user is writing for a New Zealand audience: emails, chat messages, proposals, client communications, blog posts, web copy, or any business writing. Apply to drafting, editing, and tone-checking professional text.

日本語の概要は準備中です。原文の説明を表示しています。

British business English writing style for professional communications — polished, understated, EN-GB spelling (colour, organise, centre). Use whenever the user is writing for a British audience: emails, chat messages, proposals, client communications, blog posts, web copy, or any business writing. Apply to drafting, editing, and tone-checking professional text.

日本語の概要は準備中です。原文の説明を表示しています。

Australian business English for professional writing — warm, direct, EN-AU spelling (colour, organise, centre), no filler words. Use whenever the user is writing for an Australian audience: emails, chat messages, proposals, client communications, blog posts, web copy, or any business writing. Apply to drafting, editing, and tone-checking professional text.

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Exhaustively extract UX patterns from a reference web app. Walks every screen, captures screenshots of every state, records interaction patterns, copy verbatim, keyboard shortcuts, responsive treatments, motion, and empty/error/loading states. Produces a reusable pattern library that other audits can compare against. The inverse of ux-audit — asks 'what is the bar?' rather than 'does this match the bar?'. Trigger with 'learn from X', 'extract patterns from X', 'study X's UX', 'reverse engineer the UX of X', 'build a pattern library from X'.

日本語の概要は準備中です。原文の説明を表示しています。