「net」の検索結果

Ensure .NET/C# code meets best practices for the solution/project.

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Manage NuGet packages in .NET projects/solutions. Use this skill when adding, removing, or updating NuGet package versions. It enforces using `dotnet` CLI for package management and provides strict procedures for direct file edits only when updating versions.

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Idiomatic C# and .NET patterns, conventions, dependency injection, async/await, and best practices for building robust, maintainable .NET applications.

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Plan, create, and configure production-ready Google Kubernetes Engine (GKE) clusters using the golden path Autopilot configuration. Covers Day-0 checklist, Autopilot vs Standard, networking (private clusters, VPC-native, Gateway API), security (Workload Identity, Secret Manager, RBAC hardening), observability, scaling, cost optimization, and AI/ML inference. WHEN: create GKE cluster, provision GKE environment, design GKE networking, secure GKE, optimize GKE cost, GKE autoscaling, GKE inference, GKE upgrade, GKE observability, GKE multi-tenancy, GKE batch, GKE HPC, GKE compute class.

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Expert DevOps engineer for CI/CD, IaC, Kubernetes, and deployment automation. Activate on: CI/CD, GitHub Actions, Terraform, Docker, Kubernetes, Helm, ArgoCD, GitOps, deployment pipeline, infrastructure as code, container orchestration. NOT for: application code (use language skills), database schema (use data-pipeline-engineer), API design (use api-architect).

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Simulate transcription factor perturbation effects on cell state in silico with CellOracle and Dynamo, and predict transcriptional responses to genetic perturbations with GEARS, scGen, and CPA. Covers the direction-not-magnitude principle, local-linear validity, the GRN/velocity error it inherits, baseline discipline (mean and additive baselines), and the validation gap. Use when predicting TF knockout or overexpression effects, ranking driver TFs for fate transitions, or planning perturbation experiments. For GRN construction see multiomics-grn; for experimental Perturb-seq see single-cell/perturb-seq.

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Analyzes species-environment relationships with constrained ordination (CCA, RDA, db-RDA), variance partitioning, indicator species (indicspecies IndVal.g group-equalized), PERMANOVA paired MANDATORILY with PERMDISP (Anderson & Walsh 2013; dispersion confounds centroid tests), Joint Species Distribution Models (HMSC, sjSDM, gjam) with explicit rejection of "residual covariance equals biotic interaction", phylogenetic community ecology (SES_MPD/MNTD), trait-environment via RLQ + fourth-corner with corrected modeltype=6 (Dray 2014), bipartite network metrics (NODF, modularity) with curveball null (Strona 2014), and Mantel-test replacements (dbRDA, GDM) for spatial data. Use when testing how environmental gradients structure communities, identifying habitat indicator taxa, partitioning variance among predictors, deciding whether PERMANOVA significance is location vs dispersion, picking among HMSC/sjSDM/gjam, or replacing Mantel tests for landscape data.

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Designs and analyzes combinatorial CRISPR screens covering paired-Cas9 (Big Papi, Najm 2018), enhanced AsCas12a multiplex (enCas12a, DeWeirdt 2021), in4mer 4-guide-array Cas12a (Esmaeili Anvar N et al 2024 Nat Commun 15:3577) and the Inzolia paralog-pair library, paralog-buffering detection (Dede 2020 Genome Biol; Thompson 2021 Nat Commun 12:1302), genetic-interaction (GI) scoring as observed_double_LFC minus expected_additive_double_LFC, synthetic-lethal and synthetic-rescue interaction interpretation, the half-of-essentiality buffered by paralogs phenomenon, multiplex screen statistical analysis with MAGeCK MLE interaction terms, and the relationship to single-cell combinatorial Perturb-seq. Use when designing a paralog or pathway-pair screen, choosing between paired-Cas9 (Big Papi) and Cas12a multiplex (Inzolia), interpreting genetic interaction scores, identifying synthetic-lethal targets for drug development, or scaling beyond single-gene CRISPR screens.

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Detect syntenic blocks and structural rearrangements between genomes using MCScanX (Wang 2012), JCVI/MCScan (Tang 2008 Python), GENESPACE (Lovell 2022) for orthology-anchored riparian visualization, SyRI for structural variation, AnchorWave for sequence-level synteny, i-ADHoRe 3.0 for highly diverged species, SynNet for synteny networks, and ntSynt for multi-genome macrosynteny. Use when identifying collinear gene blocks across species, distinguishing macrosynteny from microsynteny, detecting inversions/translocations/duplications, anchoring orthology in WGD lineages, producing publication riparian plots, computing synteny block age via Ks (cross-references whole-genome-duplication), or running synteny-aware ortholog inference in polyploids.

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Build whole-genome alignments using Progressive Cactus (Armstrong 2020 reference-free clade-level WGA), Minigraph-Cactus (Hickey 2024 pangenome-aware), LASTZ chain/net (UCSC pipeline), MUMmer4 (Marçais 2018 pairwise), minimap2 -x asm5/10/20 (Li 2018 fast pairwise), AnchorWave (Song 2022 WGD-aware), and Mauve / progressiveMauve (bacterial). Operates the HAL toolkit (Hickey 2013) for downstream extraction including halSynteny, halLiftover, halBranchMutations, and hal2maf. Use when constructing multi-species alignments for comparative-annotation projection (TOGA), synteny detection, conservation analyses (phyloP / PhastCons), or pangenome graph construction; selecting between reference-free (Cactus) and reference-anchored (LASTZ chains/nets) approaches; tuning sensitivity for closely vs distantly related genomes; or producing HAL files for genome-wide downstream tools.

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Detect horizontal gene transfer (HGT / LGT) using compositional methods (GC%, codon usage, tetranucleotide z-scores via SIGI-HMM, AlienHunter, IslandViewer 4, IslandPath-DIMOB), phylogenetic-incongruence methods (AvP, HGTphyloDetect, ALE / GeneRax / AleRax reconciliation, RANGER-DTL), and BLAST-distribution methods (HGTector v2, DarkHorse, Alien Index). Use when screening prokaryote genomes for genomic islands and HGT events, distinguishing HGT from incomplete lineage sorting / differential gene loss / hybridization, mapping donor lineages via phylogenetic placement, separating eukaryotic HGT from contamination, ruling out gBGC as a false signal, or quantifying DTL rates with ALE/GeneRax on bacterial trees.

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Estimates SNP heritability and partitions it across functional annotations, cell types, and loci from GWAS summary statistics or individual-level genotypes. Implements LDSC, stratified LDSC with the baseline-LD model, Finucane 2018 cell-type prioritization, LDAK SumHer, HDL, HESS local heritability, BOLT-REML, GCTA-GREML, graphREML, and Popcorn cross-population genetic correlation. Use when computing total h2_SNP from summary stats, partitioning heritability across functional categories, prioritizing trait-relevant tissues or cell types from ENCODE/Roadmap chromatin marks, reconciling LDSC vs LDAK enrichment estimates, computing local heritability with HESS, estimating genetic correlation between traits, or producing publication-grade enrichment with calibrated sensitivity to model assumptions.

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Guidance for SPR and BLI binding characterization experiments. Use when: (1) Planning binding kinetics experiments, (2) Troubleshooting poor/no binding signal, (3) Interpreting kinetic data artifacts, (4) Choosing between SPR vs BLI platforms.

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Infers genetic super-population ancestry from a 23andMe/AncestryDNA file and computes ancestry-stratified odds ratios with an exploratory Ancestry Elevation Score (AES) showing where ancestry-specific GWAS effect sizes diverge from European reference estimates.

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Expert-level geophysics covering seismic methods, gravity and magnetics, electromagnetic methods, heat flow, Earth structure, and exploration geophysics.

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Expert-level applied electromagnetics covering transmission lines, waveguides, antennas, microwave circuits, EMC, and computational EM methods.

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Expert-level graph theory knowledge. Use when working with graph algorithms, network flows, matching, graph coloring, planar graphs, spectral graph theory, random graphs, or graph applications in computer science and networks. Also use when the user mentions 'shortest path', 'minimum spanning tree', 'network flow', 'bipartite matching', 'graph coloring', 'clique', 'independent set', 'vertex cover', 'planarity', 'graph isomorphism', 'adjacency matrix', or 'Laplacian'.

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Use when choosing .NET concurrency patterns for async I/O, queues, pipelines, or thread safety. Keywords: async/await, channels, dataflow, Rx, lock contention, producer consumer, parallel processing.

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Use when verifying a story or ticket implementation against its spec and acceptance criteria - per-AC verdicts with file:line evidence and proving tests, code reuse and design conformance, dead code, stale docs, comment discipline. Finds the spec from an explicit path, the ticket key in the branch name, or plan files - and asks rather than guessing. For defect-hunting code review use dotnet-techne-code-review instead. Keywords: story QA, acceptance criteria, spec conformance, AC coverage, definition of done, dead code check, reuse check, comment check, verify story, QA this branch.

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Use when designing types and collections for hot paths and low-allocation .NET code. Keywords: readonly struct, sealed class, ValueTask, Span, FrozenDictionary, FrozenSet, allocation optimisation.

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Use when you need blast-radius analysis, dependency graphs, cross-repo impact, breaking-change diff, or architectural overview of .NET workspaces. Keywords: blast radius, dependency graph, impact analysis, cross-repo, call graph, endpoint map, EF Core lineage, breaking change, daemon, reindex.

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Use when reviewing a .NET pull request for breaking changes that may affect other microservice repositories. Detects cross-repo API, DTO, endpoint, EF entity, and NuGet-package breaks, and checks whether a compatible downstream PR already exists. Keywords: cross-repo impact, breaking change, microservice compatibility, downstream break, compatible PR, blast radius review, api compatibility, contract break, dto shape change, endpoint break.

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Use when reviewing PRs/diffs/branches/documents for .NET quality, correctness, performance, security, data access, messaging, and observability. Includes adversarial critical-thinking mode for skeptical/cynical review requests. Keywords: code review, review PR, review diff, critical review, cynical review, adversarial review, production readiness, low GC, AOT, security review.

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Use when designing or changing public C#/.NET APIs with compatibility and versioning constraints. Keywords: breaking change, API design, backward compatibility, binary compatibility, deprecation strategy, versioning.

日本語の概要は準備中です。原文の説明を表示しています。