9,365 件 ・ 関連度順
概要と使いどころ
Biopython is a comprehensive set of freely available Python tools for biological computation. It provides functionality for sequence manipulation, file I/O, database access, structural bioinformatics, phylogenetics, and many other bioinformatics tasks.
日本語の概要は準備中です。原文の説明を表示しています。
ClickHouse database patterns, query optimization, analytics, and data engineering best practices for high-performance analytical workloads.
日本語の概要は準備中です。原文の説明を表示しています。
Generate comprehensive C4 architecture documentation for an existing repository/codebase using a bottom-up analysis approach.
日本語の概要は準備中です。原文の説明を表示しています。
AI operational modes (brainstorm, implement, debug, review, teach, ship, orchestrate). Use to adapt behavior based on task type.
日本語の概要は準備中です。原文の説明を表示しています。
Backend architecture patterns, API design, database optimization, and server-side best practices for Node.js, Express, and Next.js API routes.
日本語の概要は準備中です。原文の説明を表示しています。
Agente que simula Bill Gates — cofundador da Microsoft, arquiteto da industria de software comercial, estrategista tecnologico global, investidor sistemico e filantropo baseado em dados.
日本語の概要は準備中です。原文の説明を表示しています。
Optimize Bazel builds for large-scale monorepos. Use when configuring Bazel, implementing remote execution, or optimizing build performance for enterprise codebases.
日本語の概要は準備中です。原文の説明を表示しています。
Azure MySQL Flexible Server SDK for .NET. Database management for MySQL Flexible Server deployments.
日本語の概要は準備中です。原文の説明を表示しています。
You are a senior backend engineer operating production-grade services under strict architectural and reliability constraints. Use when routes, controllers, services, repositories, express middleware, or prisma database access.
日本語の概要は準備中です。原文の説明を表示しています。
Connect to Azure Database for PostgreSQL Flexible Server from Node.js/TypeScript using the pg (node-postgres) package.
日本語の概要は準備中です。原文の説明を表示しています。
Validates animation durations, enforces typography scale, checks component accessibility, and prevents layout anti-patterns in Tailwind CSS projects. Use when building UI components, reviewing CSS utilities, styling React views, or enforcing design consistency.
日本語の概要は準備中です。原文の説明を表示しています。
Azure PostgreSQL Flexible Server SDK for .NET. Database management for PostgreSQL Flexible Server deployments.
日本語の概要は準備中です。原文の説明を表示しています。
Azure Cosmos DB SDK for Java. NoSQL database operations with global distribution, multi-model support, and reactive patterns.
日本語の概要は準備中です。原文の説明を表示しています。
Azure Identity SDK for Rust authentication. Use for DeveloperToolsCredential, ManagedIdentityCredential, ClientSecretCredential, and token-based authentication.
日本語の概要は準備中です。原文の説明を表示しています。
Azure Maps SDK for .NET. Location-based services including geocoding, routing, rendering, geolocation, and weather. Use for address search, directions, map tiles, IP geolocation, and weather data.
日本語の概要は準備中です。原文の説明を表示しています。
Build real-time web applications with Azure Web PubSub SDK for Java. Use when implementing WebSocket-based messaging, live updates, chat applications, or server-to-client push notifications.
日本語の概要は準備中です。原文の説明を表示しています。
Core UI/UX engineering skill for building highly interactive, spatial, weightless, and glassmorphism-based web interfaces using GSAP and 3D CSS.
日本語の概要は準備中です。原文の説明を表示しています。
Deploy web apps with backend APIs, database, and file storage. Use when the user asks to deploy or publish a website or web app and wants a public URL. Uses HTTP API via curl.
日本語の概要は準備中です。原文の説明を表示しています。
Automate Airtable tasks via Rube MCP (Composio): records, bases, tables, fields, views. Always search tools first for current schemas.
日本語の概要は準備中です。原文の説明を表示しています。
Assign cell types from marker expression in IMC/MIBI data using clustering (PhenoGraph/FlowSOM/Leiden/Pixie), marker-based probabilistic classifiers (Astir), or image-context CNNs (CellSighter), covering the double-positive segmentation artifact, lineage-vs-state markers, the two spillover types, and why a "cell type" in imaging is conditioned on a segmentation guess. Use when phenotyping segmented IMC cells, choosing clustering vs classification, diagnosing implausible double-positive populations, separating lineage from functional markers, or transferring labels across a cohort.
日本語の概要は準備中です。原文の説明を表示しています。
Designs donor/repair templates for precise CRISPR knock-ins -- choosing the format (ssODN, long-ssDNA/Easi-CRISPR, dsDNA/plasmid, AAV6), sizing homology arms, placing the cut within ~10 bp of the edit, and adding a mandatory codon-checked blocking (PAM/seed) mutation so the edited allele is not re-cut. Frames the HDR-vs-NHEJ-vs-MMEJ pathway competition, the MMEJ (PITCh) and homology-independent (HITI/HMEJ) alternatives for post-mitotic cells, ssODN strand/asymmetry choice, phosphorothioate end-protection, and ranked HDR enhancers. Use when designing a donor for a point mutation, epitope/fluorophore tag, allele replacement, or knock-in, or when HDR efficiency is low. Guide design and base/prime editing are separate skills.
日本語の概要は準備中です。原文の説明を表示しています。
Designs and ranks guide RNAs (sgRNAs) for CRISPR-Cas9/Cas12a gene knockout by scanning a target for PAM sites (NGG SpCas9, NNGRRT SaCas9, TTTV Cas12a, NG SpCas9-NG, near-PAMless SpRY), enumerating candidate spacers, applying hard filters (Pol-III TTTT terminator, 5' G, GC), ranking on-target activity with the context-appropriate model (Rule Set 2/Azimuth for U6/lentiviral, CRISPRscan for T7/embryo, DeepHF for high-fidelity variants, DeepCpf1 for Cas12a), and predicting the indel/frameshift outcome (Bae out-of-frame score, inDelphi, FORECasT, Lindel). Use when selecting sgRNAs to knock out a gene, choosing a nuclease/PAM for a constrained locus, picking which exon to target, or shortlisting guides before an off-target check. Off-target specificity, base/prime editing, and HDR donors are separate skills.
日本語の概要は準備中です。原文の説明を表示しています。
Assembles genomes de novo from noisy long reads (Oxford Nanopore R9/R10/Dorado, PacBio CLR) with Flye (repeat graph), Canu (correct-trim-assemble OLC), NextDenovo, Shasta, Raven, wtdbg2, or miniasm, and reconciles bacterial assemblies into a consensus with Trycycler/Autocycler. Covers matching the input flag to the basecaller era (--nano-hq vs --nano-raw), why a raw long-read assembly is contiguous but low-QV and not finished until polished, haplotig false-duplication and purge_dups, coverage and read-N50 as non-substitutable inputs, and mid-read adapter de-chimerization. Use when assembling a bacterial or eukaryotic genome from ONT or PacBio noisy reads, choosing a long-read assembler, or diagnosing an over-collapsed or duplicated assembly. For PacBio HiFi use hifi-assembly instead.
日本語の概要は準備中です。原文の説明を表示しています。
Decides whether and how to polish a draft genome assembly to raise consensus accuracy (QV) with read-type-matched tools - Racon and medaka (ONT consensus), dorado polish, Polypolish and pypolca (Illumina, repeat-aware), Pilon (legacy short-read), NextPolish/NextPolish2, Hapo-G (haplotype-aware), ntEdit, and DeepPolisher/PEPPER-Margin-DeepVariant for human. Covers the do-not-polish-HiFi rule, the medaka basecaller-model footgun, held-out Merqury QV as the only honest stop signal, and the haplotype-collapse trap. Use when correcting homopolymer indels or residual SNPs in a long-read assembly, deciding if a HiFi assembly needs polishing, or choosing an ONT vs hybrid vs short-read polishing chain.
日本語の概要は準備中です。原文の説明を表示しています。