「troubleshooting」の検索結果

Performs differential expression on bulk RNA-seq count data with DESeq2's negative-binomial GLM, Wald and LRT testing, apeglm/ashr/normal LFC shrinkage, independent filtering, Cook's outlier handling, VST/rlog transforms, and design formulas including paired, batch, and interaction terms. Use when running bulk DE, choosing DESeq2 over edgeR or limma-voom, building a paired or interaction design, applying LFC shrinkage for ranking or GSEA, choosing Wald vs LRT, troubleshooting padj=NA, picking VST vs rlog, importing salmon/kallisto via tximport, or analyzing prokaryotic RNA-seq.

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Creates DE-specific diagnostic and result visualizations using DESeq2/edgeR built-in functions and lightweight ggplot2 wrappers. Covers MA plot (with the shrunken-LFC compression effect), volcano (with the apeglm caveat that p-values are unchanged), PCA on VST/rlog (never raw counts), sample distance heatmaps, top-DE-gene heatmaps with the row-scaling trap, dispersion / BCV plot interpretation, p-value histogram diagnostics, plotCounts for individual genes, blind=TRUE vs FALSE rationale, and the n=3 visualization stake. Use when generating DE diagnostic plots, choosing VST vs rlog for visualization, troubleshooting suspicious plot patterns (shifted MA cloud, batch-dominated PCA, anti-conservative p-value histogram), or building a standard QC figure panel.

日本語の概要は準備中です。原文の説明を表示しています。

Extracts, filters, annotates, and exports differential expression results from DESeq2 or edgeR with proper handling of padj=NA (independent filtering, Cook's outliers, all-zero), multiple-testing correction choice (BH vs Storey q-value vs IHW vs lfsr), TREAT vs post-hoc fold-change filtering, p-value histogram diagnostics, gene annotation via org.db/biomaRt/mygene, GSEA preranked input, ORA background construction, replication reality (Schurch 2016 small-n result), and SABV/sex-stratified reporting. Use when extracting and interpreting DE results, troubleshooting padj=NA, choosing FDR method, preparing ranked lists for pathway analysis, annotating gene IDs, or comparing DESeq2 vs edgeR outputs.

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Analyzes CRISPR drug-modifier (chemogenomic) screens with drugZ (Li & Hart 2019 Genome Med), a bidirectional Z-score method that identifies synthetic-lethal sensitizing genes and resistance-conferring suppressor genes from vehicle vs drug comparisons. Covers vehicle-anchored design (not Day-0), the bidirectional Z math giving 2-3x sensitivity over MAGeCK / STARS / edgeR / RIGER on drug screens, per-gene sumZ and normZ, synth (sensitizer) vs supp (suppressor) FDR, multi-dose handling, integration with control sgRNAs, and comparison with MAGeCK MLE with dose covariate. Use when running a drug-modifier CRISPR screen, identifying sensitizing or resistance genes for a drug candidate, choosing drugZ vs MAGeCK MLE for chemogenomic analysis, troubleshooting low-effect drug screens where MAGeCK lacks sensitivity, or designing a drug-screen layout (vehicle vs drug arms).

日本語の概要は準備中です。原文の説明を表示しています。

Assesses RNA-seq data quality specifically for alternative splicing analysis. QC layers include experimental design audit (library prep, read length, depth, replicates), STAR 2-pass cohort-style alignment, junction saturation curves and discovery plateau detection, novel-vs-known junction ratio diagnostics, junction-overhang distribution, splice-site strength scoring (MaxEntScan intrinsic + SpliceAI context-aware), strandedness verification, GENCODE basic vs comprehensive choice, and rRNA contamination screening. Splicing analysis is more demanding than DGE on read length, depth, library prep, alignment strategy, and annotation choice — failures silently bias PSI estimates and inflate novel-junction false positives. Use when evaluating data suitability for splicing analysis, troubleshooting low event detection, or designing sequencing experiments where AS is a primary endpoint.

日本語の概要は準備中です。原文の説明を表示しています。

Add MAUI DevFlow to a .NET MAUI project with agent package references, MauiProgram.cs registration, Blazor WebView support, GTK variants, Central Package Management guidance, and verification commands. USE FOR: first-time DevFlow setup, reviewing what files to edit, choosing DevFlow packages, or continuing after `maui devflow init` installs skills. DO NOT USE FOR: troubleshooting an already-integrated app that cannot connect, iterative app debugging, UI inspection, or generic MAUI build failures (use maui-devflow-debug). INVOKES: maui devflow CLI and dotnet CLI.

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Delegate a read-only Coval evaluation, simulation, monitoring, product-how-to, or troubleshooting subtask to Sofia through the official Coval MCP consult_sofia tool. Use when the user needs Coval-specific FDE judgment, analysis of organization data, metric or test-set recommendations, or a diagnosis grounded in Coval runs and conversations.

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Integrate and maintain Robelest Convex Auth in apps by always checking upstream before implementation. Use when adding auth setup, updating auth wiring, migrating between upstream patterns, or troubleshooting @robelest/convex-auth behavior across projects.

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Integrate Convex static self hosting into existing apps using the latest upstream instructions from get-convex/self-hosting every time. Use when setting up upload APIs, HTTP routes, deployment scripts, migration from external hosting, or troubleshooting static deploy issues across React, Vite, Next.js, and other frontends.

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Development commands for OneKey monorepo. Use when running dev servers, building apps, linting, testing, or troubleshooting build issues. Triggers on yarn, dev, build, lint, test, desktop, mobile, web, extension, ios, android, compile, bundle.

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Describes the NemoClaw plugin and blueprint architecture and how they orchestrate the OpenClaw sandbox. Use when looking up architecture, plugin structure, or blueprint design. Trigger keywords - nemoclaw architecture, nemoclaw plugin blueprint structure, nemoclaw cli commands, nemoclaw command reference, nemoclaw network policy, sandbox egress control operator approval, nemoclaw troubleshooting, nemoclaw debug sandbox issues.

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Build with OpenAI's stateless APIs - Chat Completions (GPT-5, GPT-4o), Embeddings, Images (DALL-E 3), Audio (Whisper + TTS), and Moderation. Includes Node.js SDK and fetch-based approaches for Cloudflare Workers. Use when: implementing chat completions with GPT-5/GPT-4o, streaming responses with SSE, using function calling/tools, creating structured outputs with JSON schemas, generating embeddings for RAG (text-embedding-3-small/large), generating images with DALL-E 3, editing images with GPT-Image-1, transcribing audio with Whisper, synthesizing speech with TTS (11 voices), moderating content (11 safety categories), or troubleshooting rate limits (429), invalid API keys (401), function calling failures, streaming parse errors, embeddings dimension mismatches, or token limit exceeded.

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Generate complete Power Platform custom connector with MCP integration for Copilot Studio - includes schema generation, troubleshooting, and validation

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Guide for using the Microsoft Fluent UI Blazor component library (Microsoft.FluentUI.AspNetCore.Components NuGet package) in Blazor applications. Use this when the user is building a Blazor app with Fluent UI components, setting up the library, using FluentUI components like FluentButton, FluentDataGrid, FluentDialog, FluentToast, FluentNavMenu, FluentTextField, FluentSelect, FluentAutocomplete, FluentDesignTheme, or any component prefixed with "Fluent". Also use when troubleshooting missing providers, JS interop issues, or theming.

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Provides expert guidance on Development Containers (devcontainers) — creating, configuring, and optimising containerised development environments using the Dev Container Specification. This skill should be used when users ask about devcontainer.json, .devcontainer setup, Dev Container Features or Templates, pre-building images, multi-container workflows, Docker-in-Docker, cloud development environments (Codespaces, DevPod), or troubleshooting container performance and permission issues.

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Comprehensive Blazor development expertise covering Blazor Server, WebAssembly, and Hybrid apps. Use when building Blazor components, implementing state management, handling routing, JavaScript interop, forms and validation, authentication, or optimizing Blazor applications. Includes best practices, architecture patterns, and troubleshooting guidance.

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Comprehensive guide for setting up and configuring CodeQL code scanning via GitHub Actions workflows and the CodeQL CLI. This skill should be used when users need help with code scanning configuration, CodeQL workflow files, CodeQL CLI commands, SARIF output, security analysis setup, or troubleshooting CodeQL analysis.

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Comprehensive expertise for Azure Static Web Apps including architecture, configuration, API integration with Azure Functions, authentication, routing, deployment, and CI/CD. Use when building, configuring, deploying, or troubleshooting Azure Static Web Apps projects with frameworks like React, Angular, Vue, Blazor, or vanilla JavaScript.

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Professional assistant for 3D printing, laser engraving, and 3D modeling workflows. Supports generating visual references for sculpting (Forger/ZBrush), creating 3D models via Meshy AI, and optimizing FDM/Resin print settings. Use when designing, fabricating, or troubleshooting physical maker projects.

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Guided setup and troubleshooting for installing, enabling, configuring, verifying, and updating @aramisfa/openclaw-a2a-outbound in OpenClaw.

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Configure and use Honcho memory with Hermes -- cross-session user modeling, multi-profile peer isolation, observation config, dialectic reasoning, session summaries, and context budget enforcement. Use when setting up Honcho, troubleshooting memory, managing profiles with Honcho peers, or tuning observation, recall, and dialectic settings.

日本語の概要は準備中です。原文の説明を表示しています。

4-phase root cause debugging: understand bugs before fixing.

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Expert network engineer specializing in modern cloud networking, security architectures, and performance optimization. Masters multi-cloud connectivity, service mesh, zero-trust networking, SSL/TLS, global load balancing, and advanced troubleshooting. Handles CDN optimization, network automation, and compliance. Use PROACTIVELY for network design, connectivity issues, or performance optimization.

日本語の概要は準備中です。原文の説明を表示しています。

Expert DevOps troubleshooter specializing in rapid incident response, advanced debugging, and modern observability. Masters log analysis, distributed tracing, Kubernetes debugging, performance optimization, and root cause analysis. Handles production outages, system reliability, and preventive monitoring. Use PROACTIVELY for debugging, incident response, or system troubleshooting.

日本語の概要は準備中です。原文の説明を表示しています。