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概要と使いどころ
Perform differential expression analysis using edgeR in R/Bioconductor. Use for analyzing RNA-seq count data with the quasi-likelihood F-test framework, creating DGEList objects, normalization, dispersion estimation, and statistical testing. Use when performing DE analysis with edgeR.
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Perform differential expression analysis using DESeq2 in R/Bioconductor. Use for analyzing RNA-seq count data, creating DESeqDataSet objects, running the DESeq workflow, and extracting results with log fold change shrinkage. Use when performing DE analysis with DESeq2.
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Visualize copy number profiles, segments, and compare across samples. Create publication-quality plots of CNV data from CNVkit, GATK, or other callers. Use when creating genome-wide CNV plots, sample heatmaps, or chromosome-level visualizations.
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Generate consensus FASTA sequences by applying VCF variants to a reference using bcftools consensus. Use when creating sample-specific reference sequences or reconstructing haplotypes.
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Create a library-grade Vue composable that accepts maybe-reactive inputs (MaybeRef / MaybeRefOrGetter) so callers can pass a plain value, ref, or getter. Normalize inputs with toValue()/toRef() inside reactive effects (watch/watchEffect) to keep behavior predictable and reactive. Use this skill when user asks for creating adaptable or reusable composables.
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Generate comprehensive pytest tests - use when generating tests, creating test suites, or testing Python code
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Generate conventional commit messages - use when creating commits, writing commit messages, or asking for git commit help
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Creates an API Gateway stage with CloudWatch logging, X-Ray tracing, throttling, WAF integration, and IAM roles following AWS best practices. Use when deploying a REST API to different environments such as dev, test, or production.
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Use when connecting your agent to external APIs, tools, or services via Gateway, or restricting tool access with Cedar policies. Handles gateway setup, target types, outbound auth (OAuth, API key, IAM), credentials, and Cedar policy authoring. Triggers on: "connect to API", "add gateway", "connect to MCP server", "Lambda tools", "OpenAPI", "gateway target", "Cedar policy", "restrict tools", "policy engine", "gateway auth error", "store API key", "outbound credential", "env var API key", "API key None after deploy", "credential not available after deploy", "should this be a gateway target", "give my agent tools", "add tools to agent". Not for inbound auth (who can call your agent) — use agents-harden. Not for debugging agent behavior — use agents-debug. Not for VPC networking errors (agent can't reach APIs due to VPC) — use agents-build. Not for creating or hosting a new MCP server project — use agents-get-started.
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Create managed Iceberg tables using Amazon S3 Tables (s3tables API namespace) with automatic compaction and snapshot management. Sets up table bucket, namespace, table, schema, Glue catalog registration, partitioning, IAM access control. Triggers on: create table, data lake table, analytics table, structured data storage, S3 Tables, Iceberg, Athena table, partitioning strategy, access permissions. Do NOT use for: importing files (use ingesting-into-data-lake), vector storage (use storing-and-querying-vectors), querying existing tables (use querying-data-lake), or locating existing table (use finding-data-lake-assets).
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Configures AWS Transit Gateway: creating a hub and attaching VPCs, segmenting traffic with route tables, centralizing egress and inspection through a hub (appliances or a Gateway Load Balancer endpoint), forcing east-west traffic between VPCs through AWS Network Firewall, connecting on-premises networks over the transit-gateway side of a Site-to-Site VPN or Direct Connect attachment (including ECMP to aggregate bandwidth across multiple VPN tunnels), peering transit gateways across Regions, migrating from a VPC peering mesh, and routing IP multicast. Applicable when connecting many VPCs through one router, isolating environments, forcing VPC-to-VPC traffic through a central Network Firewall, reaching on-premises over the hub, linking Regions, or moving off a peering mesh. Not applicable for single-VPC routing, VPC peering between two VPCs (vpcpeering skill), Direct Connect gateway or virtual interface setup (directconnect skill), or Route 53 DNS work.
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Configures AWS Site-to-Site VPN: creating an IPsec VPN connection between an on-premises network and a VPC, choosing the target gateway (virtual private gateway, transit gateway, or AWS Cloud WAN), choosing static or dynamic (BGP) routing, sizing tunnel bandwidth (Standard 1.25 Gbps or Large 5 Gbps), connecting many sites through a VPN Concentrator, applying the customer gateway device configuration, making a connection highly available, and monitoring tunnels with CloudWatch. Applicable when the user wants to connect a data center or branch office to AWS over an encrypted tunnel, choose how routes are exchanged, scale throughput, consolidate sites, or diagnose a down tunnel. Routes to the right per-task procedure in references. Not for AWS Direct Connect (its own service), Client VPN for individual remote users, the transit gateway side of a VPN attachment (transitgateway skill), or Route 53 DNS work.
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Creates a production-ready VPC with public and private subnets across multiple Availability Zones, including internet gateway, NAT gateways, route tables, and security groups following AWS Well-Architected principles. Use when deploying multi-AZ VPC infrastructure with automatic CIDR planning and DNS resolution.
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Onboards RCS Business Messaging and Notify OTP via the pinpoint-sms-voice-v2 AWS CLI. Covers creating a branded RCS test agent (logo, banner, rich cards, suggested replies), adding verified testers, and sending/receiving the first RCS message; and configuring AWS End User Messaging Notify to send one-time-passcode (OTP) verification codes through AWS-managed phone numbers without buying a number or completing carrier registration. Also discovers which registration type a country requires from live API definitions and runs the universal registration lifecycle (create, derive fields, fill, submit, recover from denial, resubmit) shared by 10DLC, toll-free, short code, sender ID, RCS country launch, and Notify tier upgrade. Applicable to RCS agent setup, RCS message send/receive, Notify OTP configuration, verification-code delivery, registration requirements by country, and registration submission. Does not cover WhatsApp (see aws-social-messaging).
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Configures AWS WAF to filter web traffic: creating web access control lists (web ACLs) on CloudFront, Application Load Balancers, API Gateway, and AppSync; AWS Managed Rules tuned in Count mode; rate-based rules for HTTP floods; IP set and geographic match rules; Bot Control (Common and Targeted); turning bot labels into a confidence signal; stripping spoofed inbound x-amzn-waf-* headers; recovering the real client IP behind a CDN; Fraud Control (account takeover and account creation fraud prevention); and logging and request sampling. Use when the user wants to protect a web application or API from common exploits, bots, credential stuffing, fake-account creation, or HTTP floods at the application layer (layer 7). Routes to the right per-task procedure in references. Do NOT use for L3/L4 DDoS protection (shieldadvanced skill), multi-account WAF rollout (firewallmanager skill), CloudFront configuration (cloudfront skill), or Route 53 health checks or records (route53 skill).
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Designs a resilience program: how to structure and standardize resilience policies across an organization, team, or portfolio (tiered policy model with availability/RTO/RPO targets and DR approach selection), and how often to run resilience activities (operational cadence). Applies when the user asks how to structure policies org-wide, what tiers/targets to set, which DR approach fits a tier, or how frequently to run assessments, FIS experiments, GameDays, or autoshift practice. Does not apply to creating or configuring a specific policy or resource for a single workload (use resilience-hub-getting-started), to step-by-step lifecycle execution (see aws-resilience-lifecycle), or to service-specific setup.
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Configures Amazon CloudFront content delivery across six workflows: when to use CloudFront and how it fits with AWS WAF, Shield, CloudFront Functions, Lambda@Edge, Route 53, and origins (creating a distribution, caching, and Flat Rate Pricing (FRP) versus pay-as-you-go pricing); managing custom-domain TLS certificates (ACM in us-east-1); configuring multi-tenant distributions; protecting origins with origin access control (OAC), VPC origins, and origin mutual TLS (mTLS); securing content with signed URLs and cookies, geographic restrictions, viewer mutual TLS, and edge token validation; and observing traffic with standard and real-time logs. Applicable when the customer wants to put CloudFront in front of content, choose pricing, lock an origin, restrict who can view content, or analyze logs. Not applicable for the Route 53 DNS side of a CloudFront custom domain or failover between distributions (see the route53-cloudfront skill), or for pure-Route 53 DNS work (see the route53 skill).
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Creates a complete Amazon Aurora database cluster with instances, handling cluster creation, instance provisioning, and Secrets Manager password management in the proper sequence. Use when setting up new Aurora MySQL or PostgreSQL clusters with production-ready configuration.
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Configures AWS Direct Connect: choosing a connection model (dedicated, hosted, or a link aggregation group) and completing the cross connect; creating private, public, and transit virtual interfaces and bringing up BGP; reaching many VPCs through a Direct Connect gateway including cross-account transit gateway associations; encrypting traffic with MACsec or a private IP Site-to-Site VPN; making the connection resilient and tuning failover; managing link aggregation groups; SiteLink; and migrating from a virtual private gateway to a transit gateway. Use when the user wants a private, consistent network link between a data center and AWS, or operates an existing Direct Connect setup and needs to extend, encrypt, or harden it. Routes to the right per-task procedure in references. Do NOT use for transit gateway route tables and attachments (transitgateway skill), Site-to-Site VPN without Direct Connect (sitetositevpn skill), or Route 53 DNS routing (route53 skill).
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Enables internet access for AWS Lambda functions deployed in VPC subnets by creating NAT Gateway infrastructure, configuring public/private subnet routing, and updating security groups. Use when a VPC-attached Lambda function cannot reach the internet.
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Sets up AWS Resilience Hub v2 from scratch: creates resilience policies with SLO targets, registers systems and user journeys, onboards services with input sources, and runs a first failure mode assessment. Applies when the user wants to get started with Resilience Hub v2, create a policy, onboard a service, or run an assessment — including creating one concrete policy with specific availability/RTO/RPO targets and a DR approach for a single service (even a tier-1 one). Does not apply to FIS experiments or ARC routing controls.
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Connects an existing AWS Lambda function to Amazon API Gateway by creating a REST or HTTP API with resource/method setup, Lambda proxy integration, permissions, and deployment. Always use this skill when connecting Lambda to API Gateway — it handles CORS, throttling, access logging, and production security hardening that are easy to miss.
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Configures EC2 instances to securely call AWS services by creating and attaching IAM roles via instance profiles, eliminating hardcoded credentials. Use when an EC2 instance needs permissions to access AWS services like S3, DynamoDB, SQS, or CloudWatch through temporary credentials.
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Establishes VPC peering connections between two VPCs for direct private network connectivity. Always use this skill when creating or managing VPC peering — it validates CIDR overlap, updates all route tables in both VPCs, configures DNS resolution, and provides security group guidance that are critical for correct connectivity.
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