「failures」の検索結果

Handles BED-format genomic intervals (BED3 through BED12, narrowPeak/broadPeak) and the coordinate-system substrate the whole interval category rests on, with bedtools (CLI) and pybedtools/pyranges/pandas (Python). Covers the 0-based half-open vs 1-based-closed convention boundary and the start-1/end-unchanged conversion, the silent failures (chrom-name mismatch, CRLF, lexicographic-vs-version sort under -sorted), genome/chrom.sizes generation, sorting contracts, BED12 block invariants, validation, makewindows, cross-assembly liftover (liftOver/CrossMap), and BED<->VCF/BAM/FASTA conversion. Use when reading, creating, validating, sorting, lifting between genome builds, or converting interval files, preparing inputs for bedtools/tabix/bigBed, or debugging an off-by-one or empty-overlap result.

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Detects m6A modifications from Oxford Nanopore direct-RNA-sequencing (ONT DRS) signal data using m6Anet (Hendra 2022 *Nat Methods* 19:1590; multiple-instance-learning neural network over DRACH 5-mer signal). Covers the required upstream pipeline (Dorado / Guppy basecalling -> minimap2 transcriptome alignment with `-ax map-ont -uf -k14 --secondary=no` -> nanopolish eventalign with `--scale-events --signal-index` (m6Anet-required) plus `--summary` / `--threads` housekeeping -> m6anet dataprep -> m6anet inference), per-site vs per-read probability interpretation including the `mod_ratio` per-site stoichiometry column, the DRACH-only modeling constraint, minimum-coverage thresholds (20-50 reads per site for stable probability estimates), multi-condition comparison via xPore (Pratanwanich 2021 *Nat Biotechnol* 39:1394), Nanocompore (Leger 2021 *Nat Commun* 12:7198), ELIGOS (Jenjaroenpun 2021 *NAR* 49:e7), and Dorado native modification calling (RNA004 chemistry, 2024+), reference-transcriptome version pinning, the cDNA-vs-DRS chemistry distinction (cDNA-Nanopore CANNOT be used for modification detection), and orthogonal validation against MeRIP / GLORI. Use when calling m6A from ONT DRS without immunoprecipitation, choosing m6Anet vs xPore vs Nanocompore vs ELIGOS vs Dorado native, interpreting per-site `probability_modified` vs `mod_ratio` vs per-read modification probabilities, comparing methylation between conditions from ONT data, deciding between m6Anet for known DRACH sites and Dorado/Remora for genome-wide screening, pinning RNA002 vs RNA004 chemistry and basecaller model versions, or troubleshooting eventalign / dataprep failures.

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Reads, writes, and converts molecular file formats (SMILES, InChI, SDF V2000/V3000, MOL2, PDB, MMTF) using RDKit and Open Babel with rigorous handling of aromaticity perception, stereochemistry, implicit/explicit hydrogens, kekulization, and salt/fragment separation. Use when loading chemical libraries, debugging parse failures, or preparing molecules for downstream standardization, descriptor calculation, or docking.

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Assesses RNA-seq data quality specifically for alternative splicing analysis. QC layers include experimental design audit (library prep, read length, depth, replicates), STAR 2-pass cohort-style alignment, junction saturation curves and discovery plateau detection, novel-vs-known junction ratio diagnostics, junction-overhang distribution, splice-site strength scoring (MaxEntScan intrinsic + SpliceAI context-aware), strandedness verification, GENCODE basic vs comprehensive choice, and rRNA contamination screening. Splicing analysis is more demanding than DGE on read length, depth, library prep, alignment strategy, and annotation choice — failures silently bias PSI estimates and inflate novel-junction false positives. Use when evaluating data suitability for splicing analysis, troubleshooting low event detection, or designing sequencing experiments where AS is a primary endpoint.

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Add MAUI DevFlow to a .NET MAUI project with agent package references, MauiProgram.cs registration, Blazor WebView support, GTK variants, Central Package Management guidance, and verification commands. USE FOR: first-time DevFlow setup, reviewing what files to edit, choosing DevFlow packages, or continuing after `maui devflow init` installs skills. DO NOT USE FOR: troubleshooting an already-integrated app that cannot connect, iterative app debugging, UI inspection, or generic MAUI build failures (use maui-devflow-debug). INVOKES: maui devflow CLI and dotnet CLI.

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Use when a carrier or driver asks about winter trucking, snow chain requirements, hot-weather precautions, summer tire failures, fuel additive use (anti-gel), idle management in extreme temperatures, weight restrictions in spring thaw, or how seasonal demand patterns affect freight rates and capacity.

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Use this skill when the user asks about Electronic Logging Device (ELD) compliance under 49 CFR 395.20-38 — ELD mandate, AOBRD vs ELD transition, ELD exemptions, ELD malfunctions, 8-day paper backup, ELD certification on FMCSA registered list, data file integrity, edits + annotations, driver portability, and how to handle ELD failures at roadside. Cite 49 CFR 395.20-395.38.

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Use this skill when the user asks about DOT audits, FMCSA compliance reviews, new entrant safety audits (NESA), focused investigations, what auditors actually ask for, audit document checklists, common audit failures, the audit ratings (Satisfactory / Conditional / Unsatisfactory), or how to prepare for an upcoming audit. Cite 49 CFR 385.

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Recommend, create, preview, and attach Coval trace-based metrics from OpenTelemetry spans. Use when a user has Coval traces and wants custom trace metrics, trace-aware LLM judge metrics with include_traces, latency, token usage, provider failures, tool behavior, vertical-specific workflow signals, or production monitoring based on trace attributes.

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Discover failure modes in Coval runs, uploaded conversations or local conversation exports using scoped sampling and transcript, audio and trace evidence. Prepare human review without inventing ground truth or launching new runs.

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Build a compact Coval test set from product requirements, observed conversation failures or a coverage gap. Design realistic scenarios with separate expected behaviors, provenance and controlled voice/persona variation.

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Doctrine for fixing TypeScript errors against generated types (database.types.ts, api-types.ts). Use when fixing tsc or type-check failures or a type-errors batch, writing supabase .rpc()/.from() calls or DB shape guards, errors mention Json/unknown/Database, or you are tempted to cast or suppress.

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next/dynamic code splitting and build-graph cost; first stop for build failures. Use before lazy-loading or deferring a heavy component, adding a dynamic import, React.lazy, or ssr: false; on 'window is not defined' or a hydration mismatch; shrinking the bundle; or on 'build failed', OOM, SIGKILL, a slower build, or 'Collecting page data'.

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Write content in David Dias's authentic voice. Use when Quill or any agent needs to create tweets, blog posts, emails, marketing copy, or any content that should sound like David wrote it. David's voice is personal and conversational, mixing professional insight with personal anecdotes. He shares failures openly, values community, and speaks directly to readers with honesty and warmth. Not corporate or overly polished.

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Query a running Convex app's logs + health in natural language (official MCP): failures, slow/expensive functions, deploy causality — scoped, evidence-backed, with a dashboard deep link.

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Generates Circuit Breaker pattern for PHP 8.5. Creates resilience component protecting against cascading failures with state management, fallback support, and metrics. Includes unit tests.

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Five Whys root cause analysis. Iteratively asks "why" to drill past symptoms to underlying causes. Use for debugging, investigating failures, or understanding why something went wrong.

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Develops iOS/iPhone applications with XcodeGen, SwiftUI, and SPM. Triggers on XcodeGen project.yml configuration, SPM dependency issues, device deployment problems, code signing errors, camera/AVFoundation capture debugging, iOS version compatibility, or "Library not loaded @rpath" framework errors. Use when building iOS apps, fixing Xcode build failures, or deploying to real devices. Not for AVPlayer playback issues (seek/scrub/frame stepping → avfoundation-reference skill).

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Full pull request lifecycle — create branches, commit changes, open PRs, monitor CI status, auto-fix failures, and merge. Works with gh CLI or falls back to git + GitHub REST API via curl.

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Build with OpenAI's stateless APIs - Chat Completions (GPT-5, GPT-4o), Embeddings, Images (DALL-E 3), Audio (Whisper + TTS), and Moderation. Includes Node.js SDK and fetch-based approaches for Cloudflare Workers. Use when: implementing chat completions with GPT-5/GPT-4o, streaming responses with SSE, using function calling/tools, creating structured outputs with JSON schemas, generating embeddings for RAG (text-embedding-3-small/large), generating images with DALL-E 3, editing images with GPT-Image-1, transcribing audio with Whisper, synthesizing speech with TTS (11 voices), moderating content (11 safety categories), or troubleshooting rate limits (429), invalid API keys (401), function calling failures, streaming parse errors, embeddings dimension mismatches, or token limit exceeded.

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Set up eval-based QA for Python LLM applications: instrument the app, build golden datasets, write and run eval tests, and iterate on failures. ALWAYS USE THIS SKILL when the user asks to set up QA, add tests, add evals, evaluate, benchmark, fix wrong behaviors, improve quality, or do quality assurance for any Python project that calls an LLM model.

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Use when feature flag tests fail, flags need updating, understanding @gate pragmas, debugging channel-specific test failures, or adding new flags to React.

日本語の概要は準備中です。原文の説明を表示しています。

Give your AI agent the same visibility you have in the Power Automate portal — plus a bit more. The Graph API only returns top-level run status. Flow Studio MCP exposes action-level inputs, outputs, loop iterations, and nested child flow failures. Use when asked to: list flows, read a flow definition, check run history, inspect action outputs, resubmit a run, cancel a running flow, view connections, get a trigger URL, validate a definition, monitor flow health, or any task that requires talking to the Power Automate API through an MCP tool. Also use for Power Platform environment discovery and connection management. Requires a FlowStudio MCP subscription or compatible server — see https://mcp.flowstudio.app

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Debug failing Power Automate cloud flows using the FlowStudio MCP server. The Graph API only shows top-level status codes. This skill gives your agent action-level inputs and outputs to find the actual root cause. Load this skill when asked to: debug a flow, investigate a failed run, why is this flow failing, inspect action outputs, find the root cause of a flow error, fix a broken Power Automate flow, diagnose a timeout, trace a DynamicOperationRequestFailure, check connector auth errors, read error details from a run, or troubleshoot expression failures. Requires a FlowStudio MCP subscription — see https://mcp.flowstudio.app

日本語の概要は準備中です。原文の説明を表示しています。