「sse」の検索結果

Comprehensive quality control for CLIP-seq libraries (eCLIP, iCLIP, iCLIP2, PAR-CLIP) covering library complexity (preseq), FRiP, IDR replicate reproducibility, read-distribution metagene, SMInput vs IgG control rationale, rRNA / snoRNA contamination, fragment-length distribution, and ENCODE-compliance thresholds. Use when assessing whether a CLIP library passed, deciding lenient vs stringent peak thresholds, comparing replicates with IDR rescue and self-consistency ratios, or distinguishing failed IP from over-amplified library.

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Align preprocessed CLIP-seq reads (eCLIP, iCLIP, iCLIP2, PAR-CLIP) to genome with STAR or bowtie2 using crosslink-preserving parameters, choosing between unique-mapper-only and multi-mapper-aware alignment for repeat-binding RBPs, deciding STAR vs HISAT2 memory trade-offs, and applying ENCODE-compatible filters. Use when turning preprocessed CLIP FASTQ into a deduplicated, MAPQ-filtered BAM ready for peak calling or crosslink-site detection.

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Corrects the gene-independent copy-number artifact in CRISPR-Cas9 screens (Aguirre 2016 / Munoz 2016 Cancer Discov) where amplified loci appear essential from DNA-damage burden of simultaneous cuts. Covers the gene-independent DNA-damage / G2-arrest mechanism, CRISPRcleanR (Iorio 2018) unsupervised pre-hoc correction, CERES (Meyers 2017) joint CN + gene-effect model, Chronos (Dempster 2021) DepMap-standard population-dynamics + CN model with lowest residual bias, the decision tree by data availability, the Spearman LFC-vs-CN diagnostic, focal-amplification examples (ERBB2 in HER2+, MYC in colorectal, FGFR1 in head and neck), and CRISPRi/a alternatives that bypass the artifact. Use when screening cancer cell lines, diagnosing essentiality at amplified loci, choosing CRISPRcleanR / CERES / Chronos, deciding whether CN correction is needed before MAGeCK / BAGEL2 / drugZ, or switching from Cas9 to CRISPRi.

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Constructs and validates polygenic risk scores using LDpred2-auto, SBayesRC, MegaPRS, PRS-CS, PROSPER, MUSSEL, BridgePRS, JointPRS, PRSmix, or PGS Catalog Calculator with ancestry-aware reference panels (HapMap3, UKB-LD), ancestry-conditional calibration, and PRS-RS reporting standards. Use when computing PRS for cohorts, applying absolute-risk transformation, assessing cross-ancestry portability (Martin 2017 / Ding 2023 continuous ancestry), or auditing PRS manuscripts against the 22-item PRS-RS reviewer checklist.

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Build and analyze pangenomes for prokaryotes (Panaroo, PPanGGOLiN, PEPPAN, GET_HOMOLOGUES, anvi'o pangenomics) and eukaryotes (Minigraph-Cactus, PGGB, vg pangenome graphs). Implement Tettelin core/accessory/cloud genome decomposition (Tettelin 2005), Heap's law open/closed pangenome modeling, gene presence/absence GWAS (Scoary, pyseer), pangenome graph variant calling (vg, PanGenie), and structural-variation graph indexing. Use when assembling species- or genus-level pan-gene catalogs, separating core from accessory/shell/cloud genes, testing gene-content associations with phenotypes, building pangenome graphs from haplotype-resolved assemblies, calling SVs from pangenome graphs, or selecting between bacterial-pangenome and eukaryotic-pangenome workflows.

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Assesses ChIP-seq quality across antibody specificity, fragmentation, enrichment, replicate concordance, and library complexity. Computes FRiP, NSC/RSC (phantompeakqualtools), library complexity (NRF/PBC1/PBC2), deepTools plotFingerprint (JS distance, AUC, synthetic JS), ChIPQC, IDR with ENCODE Nself/Nt rules, and detects hyper-ChIPable artifacts. Use when validating an antibody, diagnosing failed peak calls, deciding whether to proceed with downstream analysis, grading against ENCODE thresholds, or auditing replicate concordance.

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ATAC-seq library quality control -- TSS enrichment, FRiP, fragment-size periodicity, library complexity (NRF/PBC1/PBC2), mitochondrial fraction, and ENCODE 4 thresholds. Use when assessing whether an ATAC-seq library passes ENCODE acceptance criteria, diagnosing transposition artefacts, comparing Omni-ATAC vs standard prep quality, or selecting which replicates to drop before peak calling.

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Assesses RNA-seq data quality specifically for alternative splicing analysis. QC layers include experimental design audit (library prep, read length, depth, replicates), STAR 2-pass cohort-style alignment, junction saturation curves and discovery plateau detection, novel-vs-known junction ratio diagnostics, junction-overhang distribution, splice-site strength scoring (MaxEntScan intrinsic + SpliceAI context-aware), strandedness verification, GENCODE basic vs comprehensive choice, and rRNA contamination screening. Splicing analysis is more demanding than DGE on read length, depth, library prep, alignment strategy, and annotation choice — failures silently bias PSI estimates and inflate novel-junction false positives. Use when evaluating data suitability for splicing analysis, troubleshooting low event detection, or designing sequencing experiments where AS is a primary endpoint.

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Quantifies alternative splicing as PSI (percent spliced in) from RNA-seq using rMATS-turbo (BAM-based event), SUPPA2 (TPM-based event), MAJIQ V3 (LSV-based Bayesian), leafcutter (annotation-free intron clusters), VAST-TOOLS (cross-species with microexon support), Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage), or IRFinder-S (intron retention coverage-aware). Distinguishes the five canonical event classes (SE, A5SS, A3SS, MXE, RI), special classes (microexons, exitrons, AFE/ALE), intron retention subtypes (canonical RI vs detained introns), and applies effective-length normalization. Use when measuring splice-site usage or isoform inclusion ratios from short-read RNA-seq.

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Use when work crosses into Apple's system surfaces rather than your own UI or data — Siri and Shortcuts, widgets, Live Activities, in-app purchase, localization, privacy prompts, alarms, timers, calendar, reminders, contacts, SharePlay, VoIP and CallKit, accessory pairing, weather, Spotlight indexing, background tasks, Background Assets, push, or push on networks with no internet.

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Writes and migrates Swift Testing framework tests with @Test, @Suite, #expect, #require, confirmation, traits, withKnownIssue, Attachment.record, processExitsWith exit tests and capture lists, Test.cancel, Issue.record warnings/manual failures, XCTest-to-Swift Testing migration, Xcode 27 interoperability modes, XCUITest UI-test boundaries, performance/snapshot boundaries, mocking, async patterns, and test organization. Use when writing tests, converting XCTest assertions such as XCTUnwrap or XCTFail, reviewing advanced Swift Testing API availability, or deciding when to keep XCTest/XCUITest.

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Creates and maintains the scenarios a LiveKit agent simulation runs, and wires the agent to consume them. Use when the user asks "what should I test", "generate simulation scenarios", "write scenarios for my agent", "add a scenario for X", "organize my scenario files", "my simulations are flaky", "the scenario hits my real database", "seed state per scenario", "it passed but booked the wrong thing", "grade the final state", or wants to stress-test a flow before shipping. Covers generating a baseline with the LiveKit scenario generator and refining it, writing the cases generation misses, phrasing simulated-user instructions so they steer reliably, splitting scenarios into sets across files, and the agent-side code that seeds deterministic state from a scenario and fails a run on its final state. To run them use running-livekit-simulations.

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Bandpass, lowpass, bandstop filter design — hairpin, coupled-line, combline, stub, SIW for frequency selection and harmonic rejection. TRIGGER: user asks to design, create, or analyze any RF filter (bandpass, lowpass, highpass, bandstop, hairpin, coupled-line, combline, stub, SIW). Invoke BEFORE writing code — filter class names differ from what you would guess. SKIP: EM simulation/S-parameter extraction of an existing filter (use matlab-analyze-em), general PCB layout assembly (use matlab-assemble-pcb-layout), material/stackup setup only (use matlab-manage-pcb-material), optimization sweeps (use matlab-optimize-pcb-design).

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Dielectric substrates, metal conductors, multi-layer stackups, and loss models (FR4, Rogers, Teflon) for RF PCB simulation. TRIGGER: user asks to set up a substrate, define dielectric properties, create a stackup, select a PCB material (FR4, Rogers, Teflon, etc.), or configure metal conductors. Invoke BEFORE writing dielectric() or metal() code — the API for named vs custom materials differs significantly. SKIP: PCB layout assembly (use matlab-assemble-pcb-layout), transmission line design (use matlab-design-pcb-transmission-line), EM analysis (use matlab-analyze-em), importing a PCB file (use matlab-read-pcb-layout).

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Connect MATLAB to Databricks via Spark (Databricks Connect) or JDBC (Database Toolbox). Use when setting up the MATLAB Interface for Databricks, configuring authentication (OauthU2M, OauthM2M, PAT), creating Spark sessions with getDatabricksSession(), reading Unity Catalog tables with server-side filtering, creating JDBC connections with databricks.JDBCConnection or StandaloneJDBCConnection, connecting to SQL Warehouses, selecting JDBC drivers (Simba/OSS), or writing data back to Databricks. Triggers on: Databricks Connect, Spark from MATLAB, getDatabricksSession, .databrickscfg, databricks.JDBCConnection, StandaloneJDBCConnection, SQLWarehouse, Databricks JDBC, Databricks cluster, large table server-side filtering.

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Generate Euro NCAP test scenarios and variants using the ADT Euro NCAP support package. Use when creating NCAP seed scenarios, generating variants, translating between drivingScenario and RoadRunner, plotting scenario descriptors, computing NCAP scores, or exporting reports. Triggers on: ncapScenario, euroAssessment, getScenario, getScenarioDescriptor, generateVariants, ScenarioDescriptor, ScenarioDescriptorPlot, ncapScore, ncapReport, exportReport, configureVUT, assessmentTable, Euro NCAP, CCRs, CCRm, CCRb, CCFtap, CCCscp, CPNA, CPFA, CBNA, variant generation.

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Use when engineering or selecting the best features for single-response classification or regression in MATLAB, whatever the data's modality — for non-tabular data it routes extraction to a domain skill, then selects, assesses, and delivers on the resulting table. Not for multi-response problems, model training, or raw data acquisition.

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Patterns for using blockedImage to process large images, harness parallel compute for image processing, and write custom adapters. Use when writing code that creates, processes, or visualizes blockedImage objects, when implementing images.blocked.Adapter subclasses, or when a user needs help with large image data. Always use this skill when working with TIFF,GeoTIFF, .svs, .ndpi, .czi or other WSI, satellite imagery or microscopy volume image formats.

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Conduct threat modeling using STRIDE methodology. Identify threats, assess risks, and design security controls. Use when designing secure systems or assessing application security.

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Perform basic penetration testing and security assessments. Use reconnaissance, vulnerability discovery, and exploitation techniques. Use when validating security controls or assessing system security.

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Calibrated interrogation of a fuzzy idea before it becomes a spec. Asks one question at a time, recommends an answer, and lands each answer in §G (goal) or §C (constraints) — unknowns parked as `?` items, never guessed. The cheapest place to kill a bad idea is before §T exists. Triggers when the user has a vague idea, says "grill me", "stress-test this", "challenge my plan", "interview me before I spec", or invokes /ck:grill. Defers the actual write to the spec skill.

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Verify claims, quotations, citations and references against live primary sources, and produce a reproducible audit record instead of an assertion. Use when asked to: check whether a quotation, verse, statistic, date, attribution or citation is accurate; verify references before publishing a report, thesis, article, slide deck or legal filing; confirm a text against an authoritative database; cross-check a claim across independent sources; build a citation register or verification appendix; find out whether two datasets agree; detect a misattributed or fabricated quotation; or audit a document's existing citations. Source-agnostic by design, with working providers for REST/JSON APIs, scraped HTML, bulk corpora, bibliographic catalogues (OpenLibrary), DOI resolution (Crossref) and reference works (Wikipedia) -- including scriptural tiers (Qur'an.com, sunnah.com and an independent hadith corpus) with full diacritic handling for Arabic, Hebrew, Greek and any script with optional marks. Covers content-addressed matching when sources disagree on numbering, graded match verdicts, byte-level integrity checks, published-string verification, and a catalogue of silent failure modes (identifier divergence, mojibake, scraper blocking, false-positive keyword rules, missing glyphs, unreliable PDF text layers).

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XIR transformation pass authoring under src/xir/passes/.

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Blender to web export workflows for 3D models and animations. Use this skill when exporting Blender models to glTF for web, optimizing 3D assets for Three.js or Babylon.js, batch processing models with Python scripts, automating Blender workflows, or creating web-ready 3D pipelines. Triggers on tasks involving Blender glTF export, bpy scripting, 3D asset optimization, model compression, texture baking, or Blender automation. Exports models for threejs-webgl, react-three-fiber, and babylonjs-engine skills.

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