「deno」の検索結果

Firebase gives you a complete backend in minutes - auth, database, storage, functions, hosting. But the ease of setup hides real complexity. Security rules are your last line of defense, and they're often wrong. Firestore queries are limited, and you learn this after you've designed your data model. This skill covers Firebase Authentication, Firestore, Realtime Database, Cloud Functions, Cloud Storage, and Firebase Hosting. Key insight: Firebase is optimized for read-heavy, denormalized data. If you're thinking relationally, you're thinking wrong. 2025 lesson: Firestore pricing can surprise you. Reads are cheap until they're not. A poorly designed listener can cost more than a dedicated database. Plan your data model for your query patterns, not your data relationships. Use when "firebase, firestore, firebase auth, cloud functions, firebase storage, realtime database, firebase hosting, firebase emulator, security rules, firebase admin, firebase, firestore, cloud-functions, serverless, backend, realtime, authentication, google-cloud" mentioned.

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World-class database schema design - data modeling, migrations, relationships, and the battle scars from scaling databases that store billions of rowsUse when "database schema, data model, migration, prisma schema, drizzle schema, create table, add column, foreign key, primary key, uuid, auto increment, soft delete, normalization, denormalization, one to many, many to many, junction table, polymorphic, enum type, index strategy, database, schema, migration, data-model, prisma, drizzle, typeorm, postgresql, mysql, sqlite" mentioned.

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AIGC 创作工作流 (AIGC 创作工作流 / AIGC Creative Workflow — 以「生成式 AI 视觉创作（图像 + 视频）的工程化创作流水线」为对象的认知操作系统：从「需求/概念（要做什么画面/镜头/风格）→ 模型与工具选型（Midjourney / Stable Diffusion·SDXL·Flux / ComfyUI / 可灵 Kling / 即梦 Jimeng / Runway / Vidu / Sora）→ Prompt 与参数设计（提示词工程、风格描述、负向、种子、CFG/步数/采样器、参考图）→ 可控生成（ControlNet / LoRA / IPAdapter / inpainting / 图生图 / 局部重绘 / 区域控制）→ 批量出图与筛选 → 精修与合成（放大/超分、修手修脸、PS/AE 后期、抠图合成）→ 图生视频/文生视频（首尾帧、运镜、时长、一致性、对口型）→ 商业交付与迭代复现（参数留存、工作流文件复用、版权与可商用判断）」的完整创作-工程-交付决策链。覆盖 (a) 第一性张力 — **可控性/工程化复现（ControlNet/LoRA/ComfyUI 节点工作流/固定种子与参数/可复现可迭代）⇄ 随机性/抽卡玄学（出图随机、prompt 玄学、抽卡刷图、靠运气）**；**审美/创意/艺术指导主导（人的审美判断、概念设计、构图、镜头语言、艺术总监能力——'模型是地板，审美是天花板'）⇄ 工具/模型/参数主导（堆模型能力、堆参数、堆 LoRA、以为换个大模型就行）**；**开源生态/本地可控（Stable Diffusion / ComfyUI / Flux / civitai / liblib 本地部署可定制可控可商用、显卡门槛）⇄ 闭源商业/易用黑盒（Midjourney / 可灵 / 即梦 / Runway 开箱即用但不可精控、按量付费、黑盒）**；**图像生成（T2I/I2I：Midjourney / SDXL / Flux）⇄ 视频生成（T2V/I2V：可灵 / Runway Gen-3 / Vidu / 即梦 / Sora，2024-2025 前沿从图迁移到视频）**；**生产力/商业量产（替代或增强传统设计/插画/电商/广告/影视分镜流程、降本提效、批量）⇄ 纯艺术创作/作者表达（个人风格、实验性、作品集）**；**效率/批量（一次出几十张筛选、商单走量、自动化 pipeline）⇄ 质量/精控（单张精修、商业可用级细节、修手修脸修穿帮）**。(b) 核心工作流 / pipeline（最标准、最易蒸高质量 + CLI 化）— 单图创作链：概念/参考收集（moodboard/风格定位/竞品）→ 模型工具选型（按可控性/风格/成本/可商用判断 MJ vs SD/Flux vs 国产）→ Prompt 与参数设计（主体/风格/光影/镜头/负向词/CFG/采样器/分辨率/种子）→ 首轮出图与方向筛选 → 可控迭代（ControlNet 控构图/姿态/线稿、LoRA 控风格/角色一致性、图生图重绘、inpainting 局部修）→ 放大超分与精修（高清放大/修手修脸/后期调色合成）→ 交付与参数留存（工作流文件/种子/参数复现）。视频创作链：分镜/脚本→首帧出图（图像链产出关键帧）→图生视频（运镜/时长/首尾帧/动态强度）→多镜头一致性（角色/场景/风格统一）→对口型/配音/配乐→剪辑合成→交付。ComfyUI 工程链：节点工作流搭建（加载器/采样/ControlNet/放大/面部修复节点）→ 参数化与复用→批量队列→工作流分享（civitai/openart workflow json）。(c) 工具栈 — 闭源图像（Midjourney v6/v7、DALL·E 3、Adobe Firefly、Ideogram、Recraft）、开源图像与底座（Stable Diffusion 1.5/SDXL/SD3、Flux.1 dev/schnell（Black Forest Labs）、ComfyUI（节点式）、Automatic1111/Forge WebUI、Fooocus）、可控生成插件（ControlNet、LoRA、IPAdapter、AnimateDiff、inpainting/outpainting、ReActor 换脸、面部修复 CodeFormer/GFPGAN、放大 ESRGAN/SUPIR/Topaz）、模型/资源社区（Hugging Face、Civitai、liblib 哩布、吐司 Tusi、openart）、视频生成（可灵 Kling、即梦 Dreamina/Jimeng、Runway Gen-3/Gen-4、Luma Dream Machine、Pika、Vidu、海螺 Hailuo、Sora、Wan 通义万相、MiniMax）、辅助（Magnific 放大、Krea、Photoshop/After Effects 后期、ChatGPT/Claude 写 prompt、reverse prompt 反推）。(d) 知识正典 — AIGC 创作 canon 横跨论文/工程/社区教程：奠基论文（DDPM、Latent Diffusion/Stable Diffusion（Rombach et al）、DiT、ControlNet（Zhang et al）、LoRA、DreamBooth、Textual Inversion、SDEdit、IP-Adapter、视频扩散 SVD/Sora 技术报告）、官方文档与模型卡（Stability AI、Black Forest Labs Flux、ComfyUI 文档、Midjourney docs、可灵/即梦官方教程、Hugging Face diffusers）、社区教程与工作流（ComfyUI 官方示例、civitai 文章、B站/YouTube 创作者长教程、openart workflow）、prompt 工程资源（Midjourney 风格库、提示词指南）。(e) figures/流派 — 模型/工具创造者（Midjourney David Holz、Stability AI Emad/Robin Rombach、Black Forest Labs（Flux，原 SD 团队）、ComfyUI comfyanonymous、ControlNet 张吕敏 Lvmin Zhang、AUTOMATIC1111、可灵/即梦/快手字节团队）、创作者 KOL 与教育者（Midjourney/SD 头部创作者、ComfyUI 工作流大神、AI 视频创作者、国内 B站/抖音 AIGC 教程作者、独立 AI 艺术家）、行业分析与评测（AI 工具评测、生成式 AI 创作生态观察者）。流派分歧：开源可控派（SD/ComfyUI/Flux 本地精控）vs 闭源易用派（MJ/可灵开箱即用）、工程化派（节点工作流/可复现/参数化）vs 玄学抽卡派（prompt 玄学/堆词/抽卡）、审美主导派（'模型是地板审美是天花板'）vs 工具主导派（堆模型堆参数）、图像派 vs 视频派、艺术创作派 vs 商业生产力派、国产工具派（可灵/即梦/liblib）vs 海外工具派（MJ/Flux/Runway）。(f) 行业话术/黑话 — 出图 / 抽卡 / 刷图 / 炼丹（训练 LoRA）/ 喂图 / 垫图（参考图/图生图）/ 控图 / 锁种子 seed / CFG / 采样器 sampler / 步数 steps / 重绘幅度 denoise / 提示词 prompt / 咒语 / 负向 negative / 权重 / tag / 大模型 checkpoint / 底模 / LoRA / ControlNet / 控线稿/openpose/depth / IPAdapter / inpainting 局部重绘 / outpainting 扩图 / 高清放大 / 超分 / 修手 / 崩手 / AI 味 / 风格 / 角色一致性 / 工作流 workflow / 节点 / 跑图 / 队列 / 文生图 T2I / 图生图 I2I / 文生视频 T2V / 图生视频 I2V / 首帧/尾帧 / 运镜 / 动态强度 / 时长 / 对口型 / 一致性 / 可商用 / 训练集 / 过拟合 / 翻车 / 穿帮。(g) 争议/批判 — 「版权与训练数据」（模型训练用未授权作品、生成图版权归属、可商用风险、洗图/伪原创）、「AI 替代创作者就业」（替代画师/插画师/设计师/摄影、行业冲击与转型）、「AI 味与审美趋同」（生成图同质化、套模板、缺乏原创审美）、「玄学 prompt 神话与割韭菜」（'咒语'神秘化、'7天 AIGC 变现/月入过万'付费课割韭菜、抽卡当能力）、「工具快速迭代与淘汰焦虑」（模型月月换、工作流频繁失效、追新疲劳）、「开源 vs 闭源之争」（可控可商用 vs 易用黑盒）、「质量天花板」（修手修脸穿帮、长视频一致性、可控性仍有限）。(h) 大量隐性创作手艺 + 审美 + 工程 + 沟通软技能（prompt 的描述功底与审美词汇、参数与采样的手感、ControlNet/LoRA 的组合控制经验、节点工作流的搭建与调试、批量筛选的审美眼力、修图合成的后期功底、镜头语言与运镜设计、角色/风格一致性的控制、商业需求的翻译与交付、版权与可商用的判断）是高 tacit、靠大量实操跑图 + 审美积累 + 工程调试经验的核心。水分极高（大量'咒语速成/一键变现/AI 绘画割韭菜'营销

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数据工程 — 数据平台从业者的认知操作系统, 覆盖把数据从源系统搬运成可靠 / 可查询 / 可信赖形态供分析 / ML / 数据产品消费的全生命周期 (生成 → 摄取 → 存储 → 转换 → 服务 + 安全/数据管理/DataOps/数据架构/编排/软件工程 六条暗流, Reis & Housley 框架): 摄取与集成 (批 + CDC 变更数据捕获 Debezium + EL 工具 Fivetran/Airbyte/Meltano/dlt + Kafka Connect + schema drift) / 存储与文件表格式 (对象存储数据湖 + 列存 Parquet/ORC/Arrow/Avro + 开放表格式 Apache Iceberg/Delta Lake/Apache Hudi + lakehouse + 分区/compaction) / 转换与建模 (ELT dbt/SQLMesh + Spark + 维度建模 Kimball + Inmon + Data Vault + 大宽表 OBT + 渐变维 SCD + 增量模型 + 语义/指标层) / 编排与工作流 (Apache Airflow/Dagster/Prefect/Mage/Kestra/Apache DolphinScheduler + DAG + 幂等 + 回填 backfill + 数据资产调度) / 批流与实时 (Apache Kafka/Apache Flink/Spark Structured Streaming/Kinesis/Pulsar/Redpanda + Lambda vs Kappa + watermark/窗口/exactly-once + 流式 SQL Materialize/RisingWave + 实时 OLAP ClickHouse/Apache Druid/Apache Pinot/StarRocks/Apache Doris) / 数仓与查询引擎 (Snowflake/BigQuery/Redshift/Databricks SQL/Trino/Presto/DuckDB/Polars + 存算分离 + MPP) / 数据质量测试与可观测性 (dbt tests/Great Expectations/Soda + 数据契约 + Monte Carlo data downtime + 新鲜度/量/schema 异常检测) / 数据治理编目与血缘 (DataHub/Amundsen/OpenMetadata/Unity Catalog + 列级血缘 + PII 分类 + 访问控制 + GDPR) / DataOps 与可靠性 (数据 CI/CD + 转换版本控制 + 环境隔离 + 幂等重处理 + 数据 SLA/SLO + 计算存储 FinOps) / 数据架构范式 (现代数据栈 + lakehouse + data mesh + data fabric + 去中心化 vs 中心化所有权) / 分析工程角色 (dbt 时代连接数据工程与分析的桥) — 不含 数据科学/ML 建模本身 (是下游消费者) / BI 仪表盘制作 (serving 下游) / 数据分析报表为终点 / 'data engineer = 跑 Hadoop 的' 过时窄化 / 通用后端应用开发 (平行学科) (Data Engineering — the cognitive operating system of practitioners who design, build, and operate the data platform: moving data from source systems into reliable, queryable, trustworthy form for analytics / ML / products, covering (a) the data engineering lifecycle (generation → ingestion → storage → transformation → serving, with the undercurrents security / data management / DataOps / data architecture / orchestration / software engineering — Reis & Housley framing), (b) ingestion & integration (batch + CDC change-data-capture with Debezium, EL tools Fivetran / Airbyte / Meltano / dlt, Kafka Connect, API + file + database sources, schema drift handling), (c) storage & file/table formats (object storage data lakes, columnar formats Parquet / ORC / Arrow / Avro, open table formats Apache Iceberg / Delta Lake / Apache Hudi, lakehouse architecture, partitioning / compaction / Z-ordering), (d) transformation & modeling (ELT with dbt / SQLMesh, Spark, dimensional modeling Kimball, Inmon CIF, Data Vault, One Big Table / wide tables, normalization vs denormalization, slowly changing dimensions, incremental models, the semantic / metrics layer), (e) orchestration & workflow (Apache Airflow, Dagster, Prefect, Mage, Kestra, Apache DolphinScheduler, DAGs, idempotency, backfills, data-aware / asset-based scheduling), (f) batch vs streaming & real-time (Apache Kafka, Apache Flink, Spark Structured Streaming, Kinesis / Pulsar / Redpanda, the Lambda vs Kappa debate, watermarks / windowing / exactly-once, streaming SQL Materialize / RisingWave, real-time OLAP ClickHouse / Apache Druid / Apache Pinot / StarRocks / Apache Doris), (g) warehouses & query engines (Snowflake, BigQuery, Redshift, Databricks SQL, Trino / Presto, DuckDB, Polars, decoupled storage & compute, MPP), (h) data quality, testing & observability (dbt tests, Great Expectations, Soda, data contracts, Monte Carlo / data downtime, freshness / volume / schema anomaly detection, unit / integration testing of pipelines), (i) data governance, catalog & lineage (DataHub, Amundsen, OpenMetadata, Unity Catalog, column-level lineage, PII / data classification, access control, GDPR / data privacy), (j) DataOps & reliability (CI/CD for data, version control of transformations, environments, idempotent reprocessing, SLAs / SLOs for data, cost / FinOps for compute & storage), (k) data architecture paradigms (modern data stack, data lakehouse, data mesh, data fabric, decentralized vs centralized ownership), (l) the analytics engineering role (the dbt-era bridge between data engineering and analysis); N

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Render Blender scenes with the right engine and settings — Cycles for photoreal, EEVEE for speed/stylized, sample counts, denoising (OptiX/OIDN), light path tuning, color management (AgX/Filmic), file output (PNG/EXR/MP4), animation rendering. Use whenever the user asks to "render this", "produce an image", "render a frame / animation", "make a final image", "save the render", or any output-generation request. Make sure to use this skill even if the user does not say "render" — also covers "make a picture", "save the result", "produce a final image", "export as image".

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Assesses physical and transition climate risk for CRE properties and portfolios. Translates hazard exposure (flood, wind, wildfire, extreme heat, sea level rise) and regulatory/market shifts into financial impacts on NOI, cap rates, insurance costs, and tenant demand. Produces dollar-denominated risk, not abstract hazard scores.

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Expert-level skill for Node.js 24+ (LTS), Bun 1.2+, and Deno 2.x backend development. Covers Express 5, Fastify 5, Hono v4, NestJS, Prisma 6, Drizzle ORM, WebSockets, BullMQ, OpenTelemetry, and microservices in English and Indonesian.

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Find the places in Anabasis where TypeSafe's Jev, a cheap calibrated System One classifier (Noul, Choice, Score over a text state), could replace or sit beside an LLM call, an embedding classifier, a prose regex, a lexical ranking or a blind-judge labelling loop, and prove each one in shadow against recorded labels before anything moves. Sweeps the tree for the five shapes, puts every candidate through the authority filter (what owns the decision, can it touch a pass, a score, a denominator or a gate) and the jev-1.13 fit filter, then writes a surface card with a runnable Bun experiment. Use when asked where Jev, TypeSafe or a System One model fits, what it could replace, or to run the autoresearch feature-discovery loop over this repository's records.

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Use after an Anabasis run, comparison, or system change and before claiming improvement. Attributes movement to one owner, reports identities and censored denominators, separates deterministic proof from model judgement, keeps measurement validity apart from observed exploitation, and states what remains unrun or provisional.

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Implement a generative diffusion model (DDPM or score-based) with noise scheduling, U-Net architecture, training loop, and sampling procedures including DDIM acceleration. Use when building a generative model for image, audio, or molecular synthesis; implementing DDPM from a research paper; adding a custom noise schedule or conditioning mechanism; replacing a GAN-based generator with a diffusion alternative; or prototyping before scaling with production frameworks like diffusers.

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Work with the @upstash/qstash TypeScript/JavaScript SDK, an HTTP-based message queue, task scheduler, and background job system for serverless and edge runtimes (Next.js, Vercel, Cloudflare Workers, Deno, Node.js). Use when publishing messages to HTTP endpoints or URL groups, running background jobs without a long-running worker process, scheduling with cron expressions, delaying messages, building FIFO queues with parallelism and flow control, configuring retries and callbacks, handling a dead letter queue (DLQ), deduplicating messages, fanning out to multiple endpoints, verifying QStash webhook signatures (Next.js App Router, Pages Router, and Edge Runtime), running a local QStash dev server, or migrating regions. Also use when the user asks for a serverless cron job, async task queue, job scheduler, delayed delivery, webhook delivery with retries, or event-driven messaging between services.

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Appwrite TypeScript SDK skill. Use when building browser-based JavaScript/TypeScript apps, React Native mobile apps, or server-side Node.js/Deno backends with Appwrite. Covers client-side auth (email, OAuth, anonymous), database queries, file uploads, real-time subscriptions, and server-side admin via API keys for user management, database administration, storage, and functions.

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从零到上线的全栈应用构建指南，基于秒悟（Meoo）平台。 触发条件： (1) 用户提到"秒悟"或"Meoo"； (2) 用户要从零构建应用，且需求可被以下架构覆盖：前端 SPA（React/Vue）+ Supabase（数据库/Auth/Storage）+ Deno 边缘函数 + AI 大模型服务； (3) 用户需要部署全栈应用（含后端进程），如 Next.js SSR、Express、FastAPI 等； (4) 用户需要把项目部署到自己的阿里云账号（ECS/RDS/SLB，费用自付） 覆盖完整生命周期：项目初始化、本地开发、云服务开通、数据库管理、边缘函数部署、CDN 发布、全栈镜像部署、沙箱代码同步、账户与权益管理。

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Builds and audits a concept-ontology for a regelrecht corpus to decide and verify reference types — cross-law source-binding vs intra-law source-binding vs open-norm leaf vs external-fact leaf — and to catch concept conflation across laws. Core principle: a value's reference type is fixed by WHO derives it, and a binding is sound only when producer and consumer denote the SAME concept; the lexical surface (the word in the text) is not the concept. Detects false friends (one term, different concepts — e.g. a register-based vs a factual "naar de omstandigheden"-based residence/ingezetene concept), orphan plain-params (a value a regulation actually derives, modeled as a brute input), negation/complement re-entry (¬X modeled as an independent leaf next to X), and duplication (one concept re-entered as several independent leafs, losing the single-source-of-truth invariant). Produces a law-level glossary and a stelsel-level concept map with sound same-concept binding edges and flagged lexical-match-but-concept-mismatch edges. Use when deciding whether a value should be cross-law/intra-law/leaf, when the same term means different things across laws, or when a binding might silently conflate concepts.

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Profiling strategy for audio CPU issues — xruns, spikes, and buffer underruns. Use when the user reports CPU overload, audio glitches under load, or performance regressions. Trigger on phrases like "I'm getting xruns", "my plugin causes CPU spikes", "dropouts under high voice count", "audio thread is too slow", "how do I profile my DSP". References audio-numerics-review for denormal slowdown and audio-dsp-review for lock contention.

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Guideline for designing, implementing, and verifying secure TypeScript and JavaScript applications following OWASP Top 10 best practices. Use when the user wants to: (1) review TypeScript or JavaScript code for security vulnerabilities, (2) design a secure Node.js, Deno, or browser application architecture, (3) implement security features (authentication, authorization, cryptography, input validation), (4) audit npm/yarn/pnpm dependencies for known vulnerabilities, (5) create security checklists or verification plans, (6) fix security bugs or harden existing TypeScript or JavaScript code, (7) set up security testing and static analysis (ESLint security plugins, Semgrep, Snyk), or (8) handle any TypeScript/JavaScript security concern including injection prevention, prototype pollution, XSS protection, SSRF prevention, secrets management, and secure deployment.

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Appwrite TypeScript SDK skill. Use when building browser-based JavaScript/TypeScript apps, React Native mobile apps, or server-side Node.js/Deno backends with Appwrite. Covers client-side auth (email, OAuth, anonymous), database queries, file uploads, real-time subscriptions, and server-side admin via API keys for user management, database administration, storage, and functions.

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Use when building APIs on Hono (Cloudflare Workers, Bun, Deno, Node), debugging route ordering, wiring middleware, validating with @hono/zod-validator, returning streaming responses, configuring CORS with credentials, handling errors via app.onError, or composing typed RPC clients. Triggers: c.env binding types, c.header + c.redirect interaction, missing await next() bugs, Set-Cookie not attaching to redirect, route-precedence surprises, JWT middleware setup, hono/client typed RPC end-to-end. NOT for Express/Fastify/Koa idioms, tRPC/GraphQL paradigms, or Next.js Route Handlers.

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Use when wrangler deploys silently fail or produce wrong artifacts, secrets upload as empty strings, custom domain DNS is not resolving, route assignment broke after rename, observability/tail logs are needed, D1/KV/R2 bindings are missing, OAuth scope errors block a command, cookies on a redirect are not attaching, or the worker exceeds CPU time. Triggers: "Tenant or user not found", "Authentication error" on wrangler d1 push, secret length is 0 after upload, route returns origin not worker, "compatibility_date too old", 1101 errors, custom_domain assignment lost on script rename. NOT for AWS Lambda / Vercel Edge / Deno Deploy (different runtimes), Cloudflare Zero Trust, or R2-specific tuning.

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Processes eDNA metabarcoding from raw paired-end reads to species tables, navigating ASV (DADA2, UNOISE3) vs OTU (swarm v2) decision (Callahan 2017 vs Schloss multi-copy-16S critique), marker/primer choice (Leray COI, MiFish 12S, 515F/806R 16S, ITS2) with primer-specific bias, OBITools3 v3 command-name break (obi stats plural; .tar.gz taxonomy), tag-jumping with dual-indexing (Schnell 2015; NovaSeq 10x MiSeq), decontam as screening-not-classifier (Davis 2018), read-counts-not-abundance critique (Lamb 2019), site-occupancy modeling (Ficetola 2015), Naive-Bayes calibration limits (Bokulich 2018), and eDNA decay (Strickler 2015). Use when going from raw eDNA FASTQ to species tables, picking marker + denoising pipeline, deciding whether read counts represent abundance, applying occupancy modeling, configuring OBITools3 v3, or interpreting decontam output. Not for clinical 16S microbiome (see microbiome/amplicon-processing).

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Map N6-methyladenosine (m6A) RNA modifications at single-nucleotide resolution using miCLIP (Linder 2015), miCLIP2 + m6Aboost machine learning (Kortel 2021), GLORI (Liu 2023, antibody-free chemical conversion), DART-seq (Meyer 2019, APOBEC1-YTH fusion), m6Anet (nanopore direct RNA), or MeRIP-seq with calibration. Use when distinguishing antibody-based from antibody-free m6A detection methods, applying the DRACH motif constraint, reconciling cross-method disagreements (DART 44% in DRACH vs GLORI), or detecting m6Am at the cap.

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Normalize read-depth copy-ratio profiles and segment them into copy-number regions using circular binary segmentation (CBS, DNAcopy), hidden Markov models, HaarSeg, and fused-lasso methods. Covers GC-content, mappability, and replication-timing (wave-artifact) bias correction, panel-of-normals/PCA denoising, diploid-baseline centering, and algorithm selection by sequencing depth and event size. Use when choosing a segmentation algorithm, correcting depth bias, diagnosing oversegmentation or a mis-centered baseline, tuning CBS or HMM parameters, or understanding why a downstream CNV caller produced fragmented or shifted segments.

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Call copy number variants with the GATK best-practices workflows — the somatic CNV pipeline (CollectReadCounts, DenoiseReadCounts with tangent normalization, ModelSegments, CallCopyRatioSegments) and the germline GATK-gCNV pipeline (DetermineGermlineContigPloidy, GermlineCNVCaller cohort/case mode, PostprocessGermlineCNVCalls). Covers panel-of-normals construction, AnnotateIntervals/FilterIntervals, allelic-count integration, and QS-based filtering. Use when integrating CNV calling into a GATK variant pipeline, calling rare germline CNVs from an exome cohort, deciding between the somatic and germline GATK workflows, or diagnosing why tangent normalization removed a real event or why gCNV output has low precision.

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Suggests repeat transsphenoidal surgery for patients with persistent hypercortisolism after initial transsphenoidal surgery when imaging shows evidence of incomplete resection or a pituitary lesion. Clinical triggers include postoperative MRI revealing residual adenoma, persistent elevated UFC or late-night salivary cortisol, and visible pituitary lesion on imaging.

日本語の概要は準備中です。原文の説明を表示しています。