「sequencing」の検索結果

CRISPResso2 for analyzing CRISPR gene editing outcomes. Quantifies indels, HDR efficiency, and generates comprehensive editing reports. Use when analyzing amplicon sequencing data from CRISPR editing experiments to assess editing efficiency.

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Start and scale networked products using Andrew Chen's "The Cold Start Problem" framework for network effects. Use when the user mentions "network effects", "chicken and egg", "cold start", "two-sided marketplace", "atomic network", "hard side", "liquidity", "critical mass", "invite-only launch", "how do I get my first users", or "the marketplace has no buyers or sellers". Also trigger when launching a marketplace, social, or collaboration product that is worthless without other users, deciding launch sequencing and seeding tactics, or diagnosing stalled network growth at scale. Covers the five stages: cold start, tipping point, escape velocity, hitting the ceiling, and the moat. For word-of-mouth virality, see contagious. For habit-driven retention, see hooked-ux.

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Runs the production schedule — sequencing and releasing work, managing work in process and changeovers, and holding the promised date when the floor and the order book disagree. Use this to build or fix a production schedule, decide what to run next, diagnose why lead times are growing while machines look busy, set up OEE or throughput measurement, or choose between make-to-stock and make-to-order for a product line.

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Designs worksheets, workbooks and practice material that actually teach — sequencing difficulty, managing cognitive load, writing instructions a child can follow unaided, and building answer keys that hold up. Use this to design or fix a worksheet or workbook, decide how much practice a skill needs, structure a page so it can be completed without an adult reading it aloud, or diagnose material that looks right and does not work.

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Plans and drives cross-functional programs to delivery — scope, sequencing, dependencies, status, risk, and the escalations that keep work moving. Use this to run a multi-team initiative, recover a program that is slipping, build a delivery plan with dependencies, structure status reporting, or diagnose why cross-team work keeps missing dates.

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Owns what gets built and why: product strategy, roadmap, discovery, user experience, and the definition of success for each release. Use this to decide what to build next, to cut scope, to turn a business goal into a product bet, to judge whether a feature is worth its cost, or when engineering and go-to-market disagree about what a release is for. Also use for roadmap sequencing and for killing work that is not earning its place.

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Upgrades an existing interface to a higher standard without rebuilding it — auditing what is there, identifying what reads as generic or unfinished, and sequencing changes by impact. Use this when a product works but looks dated or default, when a redesign is being considered, when deciding whether to restyle or rebuild, or when a screen needs to look substantially better with limited effort.

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Builds and interrogates a project schedule — logic-driven sequencing, dependency types and lags, float and the critical path, resource loading and leveling, schedule risk analysis, and measuring progress against a baseline rather than against optimism. Use this to construct a schedule, audit one you have inherited, find out why a plan keeps slipping, work out what a date change actually costs, or judge whether a reported percentage complete means anything.

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Plans the shape and size of the workforce — demand for roles, build-versus-buy, attrition, and sequencing hiring against budget. Use this to build a hiring plan, decide which roles to open and in what order, model attrition and backfill, plan for a growth or contraction scenario, or connect headcount plans to the budget.

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Applies decision science and cognitive bias research to marketing and product decisions — how people actually choose under uncertainty, and how framing, defaults, sequencing, and social context change behavior. Use this to diagnose why a well-argued offer is not converting, to structure choices and pricing presentation, to design an experience around how attention and memory work, or to pressure-test whether a persuasion tactic is legitimate or manipulative.

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Builds the marketing plan of record — objectives, channel mix, budget allocation, sequencing, and the measurement that says whether it worked. Use this for annual or quarterly planning, when budget must be allocated or defended, when marketing activity feels busy but undirected, or when a plan needs pressure-testing before commitment.

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Takes something built and gets it into the market — tiering the launch to match what it actually warrants, sequencing internal readiness before external announcement, preparing sales and support to answer the questions it creates, choosing the date for a reason, and measuring adoption rather than announcement reach. Use this to plan a launch, right-size one that is consuming more than it deserves, work out why a released feature nobody uses was launched loudly, or run the weeks after launch day.

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Designs a coordinated multi-channel campaign or product launch around one story — objective, message, channel sequencing, timeline, assets, and the checklist that gets it out the door. Use this to plan a launch or campaign, sequence a go-to-market push, pressure-test a campaign before committing budget, or turn a product change into a coordinated set of activity. For ongoing channel programs rather than a bounded push, use `marketing-planning`.

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Decides whether and how to enter a new market — sizing demand from the bottom up rather than from a market report, testing whether your advantage transfers, choosing between organic entry, partnership and acquisition, sequencing the operational and regulatory work that entry actually requires, and setting the criteria that would tell you to stop. Use this to evaluate a new geography, segment or vertical, pressure-test an entry plan, or work out why a launched market never reached scale.

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Detect copy number variations from whole genome sequencing data and generate publication-quality genome-wide CNV plots. Supports CNV calling, segmentation, and visualization for cancer genomics and rare disease analysis.

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Interpret Alpha and Beta diversity metrics from 16S rRNA sequencing results.

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Designs cell-based and animal-based validation plans that translate computational, omics, biomarker, genetic, or clinical findings into experimentally testable validation routes. Always use this skill whenever a user wants to move from an in silico, statistical, or clinical association finding toward wet-lab validation using cell systems, organoid-like systems, xenograft or genetically relevant animal models. It should define the exact claim to test, separate mechanism-testing from association-support and translational-support goals, choose the best-fit model family, specify perturbation strategy, readouts, controls, sequencing of experiments, and four workload configurations (Lite / Standard / Advanced / Publication+) with one recommended primary plan. Never fabricate model availability, reagent availability, species relevance, assay feasibility, phenotype penetrance, expected effect sizes, validation success, or literature references.

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Apply before writing logic: choosing core types and data structures, sequencing scaffold-vs-feature work, asking what concurrent actors share. Get the data structures right so downstream code becomes obvious.

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Apply when sequencing an addition, refactor, or rewrite. Remove dead code, redundant validators, and stub references first, then build on the simpler base.

日本語の概要は準備中です。原文の説明を表示しています。

Apply before writing logic: choosing core types and data structures, sequencing scaffold-vs-feature work, asking what concurrent actors share. Get the data structures right so downstream code becomes obvious.

日本語の概要は準備中です。原文の説明を表示しています。

Apply when sequencing an addition, refactor, or rewrite. Remove dead code, redundant validators, and stub references first, then build on the simpler base.

日本語の概要は準備中です。原文の説明を表示しています。

Project orchestrator for the screenwriting skill set (剧本项目主线调度 / 状态存档 / story bible) — a meta-skill that adds no new craft knowledge but routes a screenplay or stage-play project through stages (premise → structure → character → scenes → draft → revision → submission) and a TV / streaming series project (电视剧 / 剧集 / pilot / 一季) through a separate series stage table (engine → character network & season arc → documents/bible → pilot structure → break story & outline → draft → submission), names which sw-* skill to invoke at each stage, defines each stage's deliverable and advisory exit check, and keeps all project state in a story-bible.md file so work can resume across sessions. Use when starting a new script project, resuming one ("continue my screenplay", "where were we"), when the user asks "what should I do next" on a script, when converting a vague idea into a full development pipeline, or when the other sw-* skills are firing individually and the overall process needs sequencing.

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Design Thinking coaching foundation knowledge: coach identity and philosophy, quality and fidelity constraints, method sequencing, coaching state schema, and the canonical deck workflow

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Implement game audio practice — bus/mixer architecture and gain in decibels, ducking (sidechain), adaptive/dynamic music via layering and re-sequencing, SFX variation, and beat synchronization. Engine-neutral. Use when the user mentions audio mixing, audio buses, adaptive/dynamic music, ducking, SFX variation, music layers, or syncing gameplay to the beat.

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