「gc」の検索結果

Predict RBP binding from RNA sequence using deep learning models (RBPNet sequence-to-signal, RNAProt RNN, GraphProt2 GCN with structure, DeepCLIP, DeepRiPe multi-modal CNN) for variant-effect prediction, in silico binding-site discovery, model interpretation, and transfer learning from CLIP and RBNS datasets. Use when computational prediction of RBP binding from sequence is needed, evaluating variant effects on binding without further wet-lab experiments, comparing model performance, or training a custom model on ENCODE eCLIP data.

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Normalize read-depth copy-ratio profiles and segment them into copy-number regions using circular binary segmentation (CBS, DNAcopy), hidden Markov models, HaarSeg, and fused-lasso methods. Covers GC-content, mappability, and replication-timing (wave-artifact) bias correction, panel-of-normals/PCA denoising, diploid-baseline centering, and algorithm selection by sequencing depth and event size. Use when choosing a segmentation algorithm, correcting depth bias, diagnosing oversegmentation or a mis-centered baseline, tuning CBS or HMM parameters, or understanding why a downstream CNV caller produced fragmented or shifted segments.

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Estimates bivariate genetic correlation (rg) between traits from GWAS summary statistics or individual-level genotypes using cross-trait LDSC, HDL, LAVA, rho-HESS, GREML-bivariate, Popcorn, and HDL-L. Use when quantifying shared genetic architecture between two traits, screening MR validity before causal inference, distinguishing global from locus-level rg, estimating trans-ancestry rg, separating partial from full causation via LCV gcp, or producing a STROBE-MR-compliant cross-trait sensitivity battery. Cross-trait LDSC intercept absorbs sample overlap and is NOT a bias; HDL is biased under sample overlap above ~5%. High rg between exposure and outcome motivates CHP-aware MR sensitivity (CAUSE, LHC-MR).

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Visualizes ChIP-seq data using deepTools (computeMatrix, plotHeatmap, plotProfile, bamCoverage, bamCompare), pyGenomeTracks (modern INI-driven track plots), Gviz (R browser-style), EnrichedHeatmap (ComplexHeatmap-based), ChIPseeker tag heatmaps, and IGV batch screenshots. Handles bigWig normalization choices (CPM, BPM, RPGC, spike-in scaled), bamCompare operations (log2 ratio, subtract) with SES scaling, k-means clustering of heatmaps for biological subgrouping, and spike-in-scaled tracks for global-shift experiments. Use when generating publication-quality ChIP-seq signal heatmaps, profile plots, genome-browser tracks, or comparing samples visually.

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Discovers de novo motifs and tests known motif enrichment in ChIP-seq, ATAC-seq, or other peak sequences using HOMER, MEME-ChIP (STREME, CentriMo, TOMTOM, FIMO), monaLisa, and AME. Handles background selection (GC-matched, dinucleotide-shuffled, Markov order-2, peak-flanks), motif databases (JASPAR 2024 CORE PWMs, JASPAR 2026 deep-learning collection, HOCOMOCO v12, HOMER built-in), centrally-enriched motif testing, and differential motif analysis. Use when identifying TF binding motifs in peaks, testing for known TF enrichment, scanning for motif instances, comparing motif content between conditions, or interpreting motifs from deep learning models.

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Estimates SNP heritability and partitions it across functional annotations, cell types, and loci from GWAS summary statistics or individual-level genotypes. Implements LDSC, stratified LDSC with the baseline-LD model, Finucane 2018 cell-type prioritization, LDAK SumHer, HDL, HESS local heritability, BOLT-REML, GCTA-GREML, graphREML, and Popcorn cross-population genetic correlation. Use when computing total h2_SNP from summary stats, partitioning heritability across functional categories, prioritizing trait-relevant tissues or cell types from ENCODE/Roadmap chromatin marks, reconciling LDSC vs LDAK enrichment estimates, computing local heritability with HESS, estimating genetic correlation between traits, or producing publication-grade enrichment with calibrated sensitivity to model assumptions.

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Validate alignment quality with insert size distribution, proper pairing rates, GC bias, strand balance, and other post-alignment metrics. Use when verifying alignment data quality before variant calling or quantification.

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Plan, execute, and document validation, verification, and transfer of analytical procedures under the governing framework - ICH Q2(R2) and Q14, USP <1220>/<1225>/<1226>, ICH M10 bioanalytical, CLSI EP, or ISO/IEC 17025. Use for HPLC, LC-MS/MS, GC, CE, ICP-MS, dissolution, qNMR, qPCR, NIR, and ligand binding or cell-based assays whenever the question is whether a procedure is fit for its intended purpose. Triggers include "method validation", "analytical method validation", "AMV", "validation protocol", "acceptance criteria", "linearity", "reportable range", "accuracy and precision", "repeatability", "intermediate precision", "recovery", "LOD", "LOQ", "detection limit", "quantitation limit", "specificity", "robustness", "method transfer", "method comparison", "Deming", "Passing-Bablok", "Bland-Altman", "equivalence testing", "OOS investigation", "ICH Q2", "Q2(R2)", "Q14", "USP 1225", "ICH M10", "incurred sample reanalysis", "ISR", "CLSI EP", and any request to show that an assay works.

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Teaches AI patients about stress-dosing and emergency glucocorticoid administration; instructs to obtain emergency card/bracelet/necklace and kit with injectable high-dose GC. Triggers when counseling any patient with adrenal insufficiency (e.g., "Use when counseling patient with adrenal insufficiency...").

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Recommends adjusting glucocorticoid doses on the day of surgery based on illness severity and magnitude of surgical stress. Triggers when managing a patient requiring surgery needing perioperative glucocorticoid management, such as "patient on HC for central AI undergoing major abdominal surgery" or "patient with hypopituitarism scheduled for minor procedure".

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Clinicians educate adrenal insufficiency patients taking nondexamethasone glucocorticoids who initiate enzyme-inducing antiepileptic drugs about early signs of adrenal insufficiency. Trigger when an AI patient on nondexamethasone GC starts an enzyme-inducing AED such as phenytoin, carbamazepine, or oxcarbazepine.

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Suggests using longer-acting glucocorticoids when standard hydrocortisone regimens are impractical due to nonavailability, poor adherence, or need for once-daily dosing. Triggers include glucocorticoid replacement therapy decisions where patients report difficulty with multiple daily doses, lack of access to hydrocortisone, or preference for simplified regimens.

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In patients with normal preoperative adrenal function, suggests an individualized clinical approach for postoperative glucocorticoid administration until HPA axis evaluation can be performed. Triggers include managing postoperative patient with normal preoperative adrenal function needing glucocorticoid management.

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Use when CPU usage is high, memory grows unboundedly, goroutines leak, mutex contention shows in traces, or escape analysis suggests excess heap allocation. Triggers: net/http/pprof endpoint exposure, go tool pprof analysis, flamegraph generation, allocs vs inuse_space heap profiles, runtime/trace event timeline, mutex profiling, block profiling, goroutine dumps, GC pressure measurement. NOT for non-Go languages, distributed tracing (use OpenTelemetry skill), or production telemetry pipelines.

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Use when designing matrix builds (OS x version x dimension), composing reusable workflows, gating jobs on conditions, doing OIDC to AWS/GCP/Cloudflare without long-lived secrets, sharding tests across runners, or fixing cache invalidation. Triggers: workflow_call between repos, fail-fast: false tradeoffs, exclude/include matrix surgery, environment protection rules, OIDC trust policy setup, concurrency cancellation on duplicate pushes, artifact reuse across jobs, conditional job matrices computed at runtime. NOT for GitLab CI / CircleCI / Jenkins (different yaml dialects), self-hosted runner administration, or local act dev.

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Data structures and serialization formats for agent-to-agent communication. Covers message envelopes, structured output schemas, capability declarations, task handoff payloads, error/retry signaling, and context windows as data structures. Deep comparison of A2A protocol, MCP, OpenAI function calling, and LangChain message types. Teaches when to use rigid schemas vs free-form with validation, typed vs untyped, streaming vs batch. Activate on: "agent message format", "agent communication schema", "agent-to-agent protocol", "A2A protocol", "MCP message format", "structured output for agents", "agent interop", "interchange format", "agent serialization", "task handoff format", "capability declaration". NOT for: what agents say to each other (use agent-conversation-protocols), orchestration topology (use multi-agent-coordination), building agent infrastructure (use agentic-infrastructure-2026).

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Create and manage online courses via the CourseForge API (caringcourseforge.com)

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Expert-level number theory knowledge. Use when working with divisibility, prime numbers, modular arithmetic, congruences, Diophantine equations, cryptography, quadratic residues, or analytic number theory. Also use when the user mentions 'prime', 'divisibility', 'modular arithmetic', 'congruence', 'GCD', 'Euler totient', 'Fermat little theorem', 'Chinese remainder theorem', 'quadratic residue', 'Diophantine equation', 'RSA', or 'prime factorization'.

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Expert-level analytical chemistry knowledge. Use when working with chromatography, spectroscopy, electroanalytical methods, sample preparation, quantitative analysis, calibration, statistics, or instrumental analysis. Also use when the user mentions 'HPLC', 'GC', 'mass spectrometry', 'atomic absorption', 'ICP', 'titration', 'calibration curve', 'detection limit', 'NMR', 'chromatography', 'electrochemistry', 'sample preparation', or 'method validation'.

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Use when reviewing PRs/diffs/branches/documents for .NET quality, correctness, performance, security, data access, messaging, and observability. Includes adversarial critical-thinking mode for skeptical/cynical review requests. Keywords: code review, review PR, review diff, critical review, cynical review, adversarial review, production readiness, low GC, AOT, security review.

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Read-only SRE checkup of any GCP project: deterministic probes of the edge, Cloud Run services, 7-day error logs, Cloud Scheduler, alert policies and uptime checks, Secret Manager and IAM, the data stores and the machine's own scheduled jobs, audited into one fixed status table (LIVE / WARNING / RED / INCONCLUSIVE) with evidence, findings by severity, what could not be checked, and a single OVERALL line delivered as one notification. Parametrised by a per-project manifest, so the same routine runs on every project. Use when the operator says "cloud checkup", "SRE check", "is everything live", "what's healthy / warning / red", "any errors this week", "audit the infra", "weekly checkup", "set up the weekly checkup", before a deploy or demo, or after an incident. Cloud Run first; App Engine and GKE differ only in the serving probes.

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Cloud guardrails for any vendor workload — Google Cloud (GCP, Vertex AI, GKE), AWS (IAM, EKS, Bedrock), Azure (Entra ID, Policy, AKS), Alibaba Cloud (RAM, mainland/international residency). Enforces identity least-privilege, mechanical policy, data boundaries, residency, cost caps, network egress and observability, with official-source validation before any claim. Trigger on any cloud infrastructure design, review, Terraform plan, or LLM/agent deployment; the-architect routes here.

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When picking a secrets store, designing rotation, or wiring scanning gates — multi-cloud (Vault, AWS, Azure, GCP), CI and Kubernetes — decision framework, provider deep-dives externalized.

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Fluxo de trabalho de infraestrutura em nuvem e DevOps abrangendo AWS, Azure, GCP, Kubernetes, Terraform, CI/CD, monitoramento e desenvolvimento nativo em nuvem.

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