1.6万 件 ・ 関連度順
概要と使いどころ
Write a polite-but-firm payment-reminder / collections email sequence for overdue invoices. Use when asked to write a collections email, a payment reminder, a dunning sequence, or to chase an overdue invoice. Produces a staged sequence — gentle pre-due nudge through escalating overdue reminders to a final notice — that stays professional, keeps the relationship intact, and makes paying easy. Not legal advice.
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Generate a tailored code review checklist for any pull request based on the language, type of change, and risk level. Use when asked to review code, check a PR, review a pull request, or generate a code review checklist. Produces a focused checklist with language-specific checks, risk-level-appropriate depth, and a clear approve/request-changes recommendation.
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Practise the structured interview (结构化面试) for Chinese civil service and public institution posts (公务员面试 / 事业单位面试): the common question types, a clear answer structure for each, timed mock rounds and feedback on content, structure and delivery. Use when asked 公务员面试怎么准备, 结构化面试题, 帮我模拟面试, 综合分析题怎么答, 应急应变题, or practise the civil service interview. Produces a question-type guide, answer frameworks with worked examples, a timed mock interview asked one question at a time, scored feedback, and a practice plan up to the interview date.
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Interview the user with sharp, one-at-a-time questions to turn a vague request into a tight, complete brief any other skill can run on. Use when a request is fuzzy, under-specified, or 'help me think this through', or before running a skill that needs inputs the user hasn't given. Produces a structured brief (goal, audience, constraints, success criteria) and hands off to the right skill — by interrogating, not guessing.
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Read Lemon Squeezy revenue: list orders, subscriptions, customers, products; create checkout links. Trigger phrases: lemon squeezy, store orders, revenue, checkout link.
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Break large features into independently verifiable, human-reviewable slices under a feature directory in specs/. Use for risky or multi-step feature work that needs upfront questioning, API seams, browser-playable checkpoints, HTML visualizations, screenshot gates, staged implementation plans, recursive fog-of-war reslicing, or proactive research into reference implementations/best practices before slicing. Pairs with your project's verification harness and screenshot gates (the browser checkpoints), [refactor-clean](../refactor-clean/SKILL.md) (review the materialized spec so the plan describes one-owner architecture, not the feature bolted on), [screenshot-critique](../../visual/screenshot-critique/SKILL.md) and [compare-screenshots](../../visual/compare-screenshots/SKILL.md) (the visual gates), and a code-review pass (audit each slice before it lands).
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Use when choosing or sharpening a research question for an Accounting, Organizations and Society (AOS) manuscript — testing whether the question genuinely concerns the social, organizational, behavioral or institutional life of accounting, and picking the intellectual tradition the question demands. Screens fit; it does not build the theory (aos-theory-development).
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Answers natural-language business questions over existing Power BI reports and semantic models through the FabricIQ MCP endpoint, discovering artifacts, resolving entities, generating the DAX itself, and returning a plain-language answer. Use when the user asks a data question, including one that says to query the model directly. For DAX the user supplies, or measures to save, use semantic-model-authoring.
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Manage Fabric Warehouse, Lakehouse SQL endpoints, and Mirrored Databases: DDL/DML, COPY INTO, read-only T-SQL, Query Insights diagnostics, and Capacity Metrics CU-spike correlation. Synapse migration target SQL belongs to synapse-migration; Fabric SQL database belongs to sqldb-cli. Triggers: query warehouse, create warehouse table, failed or canceled query, CU spike, Capacity Metrics app, custom SQL pool, Lakehouse table health.
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For churned accounts, research what has changed since they left — new funding, team growth, competitor dissatisfaction, product updates that address their pain — then assess re-engagement potential and generate a personalized win-back email sequence with timing recommendations. Chains web research and LinkedIn monitoring with email sequence generation.
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Email campaign/sequence performance review composite. Pulls campaign data (sends, opens, replies, bounces), reads actual email copy and subject lines, analyzes reply content (objections, positive interest, questions), and produces a diagnostic report covering quantitative metrics, copy quality, lead quality, and actionable recommendations. Tool-agnostic — works with Smartlead (MCP), Instantly, Outreach, Lemlist, Apollo, or CSV data.
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Estimate frequency response from Simulink models using frestimate. Use when frequency response should be obtained from simulation rather than model linearization.
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Reads, queries, and writes bigWig indexed binary signal tracks (coverage, fold-change, conservation, methylation-rate) with pyBigWig (Python) and the UCSC Kent tools (bedGraphToBigWig, bigWigToBedGraph, bigWigInfo, bigWigSummary, bigWigAverageOverBed) and deepTools (multiBigwigSummary, computeMatrix, bigwigCompare). Covers the central trap that a wide query returns a precomputed zoom-level summary (by default the mean, which annihilates narrow peaks) not per-base data, when exact=True/values() is mandatory, the NaN-not-zero gap-handling fork, choosing mean vs max vs sum vs coverage by biological question, and the sorted-bedGraph plus chrom.sizes build requirement. Use when extracting signal at regions, computing mean signal per gene/peak, building a browser track from bedGraph, comparing tracks, or building TSS/gene-body metaprofiles.
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Parses, queries, converts, and extracts from GTF and GFF3 gene-model annotation files - walking the gene/transcript/exon/CDS hierarchy with gffutils (queryable SQLite DB), converting formats and extracting transcript/CDS/protein FASTA with gffread, slurping to dataframes with gtfparse/pyranges, and sanitizing malformed files with AGAT. Covers the 1-based-inclusive vs 0-based BED coordinate conversion (start-1 only), deriving implicit features (introns/UTRs/TSS), phase-not-frame, the stop-codon-in-or-out-of-CDS convention, and the chr1-vs-1 seqid and gene-ID-version mismatches that silently produce all-zero count matrices and dropped joins. Use when extracting features or sequences from an annotation, converting GTF<->GFF3 or GTF->BED, traversing the gene tree, or diagnosing a coordinate/provenance mismatch upstream of counting or DE.
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Estimates the minimum biological replicates (or cells/events) for a target power at a target FDR in genomics experiments using ssizeRNA, PROPER, powsimR for scRNA-seq, and pilot-data dispersion estimation from DESeq2/edgeR. Covers the biological-versus-technical replication distinction (technical replicates do not add degrees of freedom for biological inference), replicate-number-versus-sequencing-depth budgeting, scRNA-seq sample-versus-cell allocation under a pseudobulk model, and the critique that "n=3" is a publication convention rather than a power calculation. Use when budgeting a sequencing experiment, writing the sample-size justification in a grant, estimating replicates from pilot data, allocating a fixed budget between samples and depth, or planning scRNA-seq cohort size. For clinical-trial sample size see clinical-biostatistics/power-and-sample-size; for the power-given-n direction see experimental-design/power-analysis.
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Bulk-query Ensembl BioMart (and other BioMart instances) for cross-database ID mapping, gene/transcript/exon coordinates, and ortholog tables. Use when batch-converting Ensembl IDs to other namespaces (HGNC, RefSeq, UniProt, Entrez), pulling gene coordinate tables for thousands of genes, building ortholog wide-tables across species, or replacing slow Ensembl REST loops with one-shot bulk export. Encodes BioMart's XML query format, R biomaRt vs Python pybiomart trade-off, mart-vs-dataset hierarchy, and the URL endpoint that's BioMart-specific (separate from rest.ensembl.org).
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Search NCBI databases using Biopython Bio.Entrez (ESearch, EInfo, EGQuery, ESpell). Use when finding records by keyword, building reproducible field-qualified queries, navigating the Entrez Query Translator, exploiting the history server for large result sets, handling retmax caps, or interpreting weekly index lag. Covers PubMed, Nucleotide, Protein, Gene, SRA, GEO, Assembly, Taxonomy, ClinVar, dbSNP.
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Build sequence logos from aligned DNA, RNA, or protein motifs using ggseqlogo (R), Logomaker (Python), or WebLogo with explicit bits vs probability encoding, background-frequency correction, custom alphabets, and multi-logo stacking. Use when visualizing motif PWMs (TF binding, splice sites, CRISPR spacers), aligned-position composition, or comparing two motif sets.
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Visualize copy number profiles, segments, allele-specific tracks, and cohort patterns from CNVkit, GATK, ASCAT, FACETS, Sequenza, and other callers. Covers genome-wide and per-chromosome log2 scatter plots, B-allele-frequency/minor-allele-fraction tracks, ideograms, cohort heatmaps, circos views, and caller-native plots. Use when creating publication CNV figures, choosing which plot answers a given question, diagnosing a wrong diploid baseline visually, displaying loss of heterozygosity, or deciding what depth-only plots cannot reveal.
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Queries myvariant.info BioThings aggregator for ClinVar, gnomAD, dbSNP, dbNSFP, COSMIC, CADD, and CIViC annotations in batched, version-tracked requests. Use when annotating variant lists from multiple databases simultaneously without managing per-source APIs, and when reproducibility-grade analyses require recording source data versions via _meta.
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Predict RBP binding from RNA sequence using deep learning models (RBPNet sequence-to-signal, RNAProt RNN, GraphProt2 GCN with structure, DeepCLIP, DeepRiPe multi-modal CNN) for variant-effect prediction, in silico binding-site discovery, model interpretation, and transfer learning from CLIP and RBNS datasets. Use when computational prediction of RBP binding from sequence is needed, evaluating variant effects on binding without further wet-lab experiments, comparing model performance, or training a custom model on ENCODE eCLIP data.
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Perform multiple sequence alignment using MAFFT, MUSCLE5, ClustalOmega, or T-Coffee. Guides tool and algorithm selection based on dataset size, sequence divergence, and downstream application. Use when aligning three or more homologous sequences for phylogenetics, conservation analysis, or evolutionary studies.
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Parse and analyze multiple sequence alignments using Biopython. Extract sequences, identify conserved regions, analyze gaps, work with annotations, and manipulate alignment data for downstream analysis. Use when parsing or manipulating multiple sequence alignments.
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Perform pairwise sequence alignment using Biopython Bio.Align.PairwiseAligner. Use when comparing two sequences, finding optimal alignments, scoring similarity, and identifying local or global matches between DNA, RNA, or protein sequences.
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