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Guidelines for making .NET libraries and applications trimming-safe and Native AOT compatible. Covers the trimming/AOT model, the MSBuild properties that enable analysis (IsTrimmable, IsAotCompatible, PublishTrimmed, PublishAot), the trimming attributes (RequiresUnreferencedCode, RequiresDynamicCode, DynamicallyAccessedMembers, UnconditionalSuppressMessage), IL2xxx/IL3xxx warning codes, and a pattern playbook: source generators and UnsafeAccessor, generated-interception diagnostic suppressors, intentional runtime scanning boundaries, trimming-safe islands and feature switches, migration analyzers, and temporary warning-approval baselines. Use when introducing trimming/AOT support, resolving IL2xxx/IL3xxx warnings, or making reflection-heavy code trimming-safe in C# / .NET codebases. For System.Text.Json AOT scenarios, see also the serialization skill.

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Download raw sequencing reads from NCBI SRA using sra-tools (prefetch, fasterq-dump, vdb-validate) or the ENA mirror. Use when pulling FASTQ for SRR/ERR/DRR accessions, deciding between SRA-direct, ENA mirror, or AWS/GCP cloud mirror (STRIDES), handling --include-technical for 10x and other single-cell records, validating with MD5/vdb-validate, navigating SRR/SRX/SRS/SRP/PRJNA hierarchy, or finding accessions via pysradb. Encodes SRA cloud-egress economics, the fasterq-dump uncompressed-scratch trap, and the --max-size default that silently truncates large prefetches.

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Build and analyze pangenomes for prokaryotes (Panaroo, PPanGGOLiN, PEPPAN, GET_HOMOLOGUES, anvi'o pangenomics) and eukaryotes (Minigraph-Cactus, PGGB, vg pangenome graphs). Implement Tettelin core/accessory/cloud genome decomposition (Tettelin 2005), Heap's law open/closed pangenome modeling, gene presence/absence GWAS (Scoary, pyseer), pangenome graph variant calling (vg, PanGenie), and structural-variation graph indexing. Use when assembling species- or genus-level pan-gene catalogs, separating core from accessory/shell/cloud genes, testing gene-content associations with phenotypes, building pangenome graphs from haplotype-resolved assemblies, calling SVs from pangenome graphs, or selecting between bacterial-pangenome and eukaryotic-pangenome workflows.

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Sequence-based deep learning for ATAC-seq using chromBPNet, BPNet, scBasset, or Enformer. Use when correcting Tn5 bias with neural networks beyond k-mer models, predicting per-base accessibility profiles, scoring in silico variant effects at GWAS or rare-variant SNPs, discovering motifs via DeepLIFT/TF-MoDISco from a trained model, or generating cell-type-specific accessibility predictions for unobserved cell states.

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Detect allele-specific chromatin accessibility from ATAC-seq using WASP, GATK ASEReadCounter, or RASQUAL. Use when mapping cis-regulatory genetic variants from heterozygous SNPs, separating cis from trans regulation, building chromatin QTL (caQTL) maps, validating GWAS variant function with allelic imbalance, or detecting reference allele mapping bias before downstream analysis.

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Use when the user mentions accessibility checking, App Store submission, code review, or WCAG compliance.

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Build direct Bluetooth Low Energy workflows with Core Bluetooth. Use when implementing BLE central or peripheral GATT communication, scanning or connecting with CBCentralManager, discovering services and characteristics, reading/writing/subscribing with CBPeripheral, publishing local services with CBPeripheralManager, handling Bluetooth authorization, background BLE modes, state restoration, write flow control, or CBUUID-based workflows. For privacy-preserving accessory setup/picker flows, use accessorysetupkit first and return here for post-setup GATT communication.

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Guide for accessing financial and economic data in MATLAB using the Datafeed Toolbox. Covers Bloomberg (market data via bloomberg/blp/bloombergHypermedia), FRED (Federal Reserve economic data via fredrs), Haver Analytics (economic data via haver/haverdirect/haverview), and LSEG Datastream (historical data via datastreamws). Use when connecting to any of these data providers from MATLAB.

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Manage users, groups, and permissions on Linux systems. Configure sudo and access controls. Use when managing system access.

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Manage IAM users, roles, and policies. Implement least-privilege access and security best practices. Use when configuring AWS identity and access management.

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CloudBase PostgreSQL access-pattern and slow-query quality guidance. Use when designing how tables are read and written, eliminating per-row database calls, adding indexes for filters or joins, reviewing high-traffic data access, or explaining a slow SQL query. Not for first-time PG SDK setup, login UI, or NoSQL collections.

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Modern web design trends, principles, and implementation patterns for 2024-2025. Use this skill when designing websites, creating interactive experiences, implementing design systems, ensuring accessibility, or building performance-first interfaces. Triggers on tasks involving modern design trends, micro-interactions, scrollytelling, bold minimalism, cursor UX, glassmorphism, accessibility compliance, performance optimization, or design system architecture. References animation skills (GSAP, Framer Motion, React Spring), 3D skills (Three.js, R3F, Babylon.js), and component libraries for implementation guidance.

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Use when bringing 3D assets into Roblox Studio or preparing avatar items: import from Blender, GLB, FBX or OBJ files, AI-generated or image-to-3D meshes (Scenario or other tools), wrong scale or size in studs, mesh faces the wrong way, too many triangles, mesh fails validation, holes or non-watertight meshes, textures too big or blurry, SurfaceAppearance and PBR maps, metalness or normal maps look wrong, rigid accessories and hats, UGC items, Accessory Fitting Tool, out of bounds, AvatarCreationService validation, EditableMesh or EditableImage previews, or generate_mesh.

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Generate complete, accessible colour palettes from a single brand hex. Produces 11-shade scale (50-950), semantic tokens, dark mode variants, Tailwind v4 CSS output, WCAG contrast checks. Use whenever the user supplies a brand hex and asks for a palette, mentions setting up a design system, wants Tailwind theme colours from a brand colour, or asks to check colour accessibility / contrast.

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Connect to a WordPress site via WP-CLI over SSH or the REST API. Check CLI, test SSH, set up auth, verify access, save config. Use whenever the user wants to connect to a WordPress site, set up WP-CLI access, create an Application Password, or troubleshoot WordPress connection / auth issues.

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The Power BI report design canon covering design identity, visual hierarchy and the 3-30-300 rule, layout/spacing/alignment, color discipline, chart selection, KPI and card design, tables and matrices, accessibility, and the closing design gate. The shared reference home for report design judgment, routed to by the create-pbi-report, pbir-cli, review-report, modifying-theme-json, and custom-visual skills. Load when committing a design identity or making a design, layout, color, chart-selection, or accessibility decision for a report.

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Expert checklist and prompts for auditing and fixing Android accessibility issues, especially in Jetpack Compose.

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Run QA testing on a page, feature, or full site at one of three depth tiers (smoke, standard, full). Use this skill whenever the user asks to QA a page or site, run a smoke test after a deploy, verify a page before launch, or run a regression sweep. Triggers on QA, QA sweep, smoke test, regression test, post-deploy check, pre-launch check, verify the deploy, test this page, does the page render, broken link, 404, image not loading. Also triggers proactively after a deploy or a new page launch where verification matters. Covers accessibility, performance, and SEO at the surface-signal level only: deep audits belong to `accessibility-audit`, `performance-optimization`, and `seo-technical`, and code-level debugging to `code-review-web`.

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Expert SOX IT General Controls (ITGC) advisor for finance, internal audit and IT compliance teams. Covers the four ITGC domains external auditors test for SOX 404 — access to programs and data, program changes, computer operations, and program development — plus scoping in-scope systems from the financial statements, designing risk-and-control matrices (RCMs), writing control narratives and test scripts, evaluating deficiencies (control deficiency vs significant deficiency vs material weakness under AS 2201 and Reg S-X), drafting remediation plans, SOX 404(a)/404(b) applicability and filer status, IPO readiness timelines, and COSO 2013 alignment. Use this skill whenever a user mentions SOX, ITGC, IT general controls, 404(a)/404(b), ICFR, material weakness, user access reviews, segregation of duties in IT, change management controls for financial systems, SOX testing or walkthroughs, PCAOB audits, or quarterly compliance for financial reporting systems.

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Senior Regulatory Affairs Manager for HealthTech and MedTech. Use for regulatory strategy, FDA 510(k)/PMA/De Novo submissions, EU MDR CE marking, global market access, and regulatory intelligence.

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Apple Human Interface Guidelines expert across iOS, iPadOS, macOS, watchOS, tvOS, and visionOS. Use when designing or reviewing an Apple-platform app, auditing for HIG compliance, picking native components, or validating accessibility.

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Pre-publication quality gate for authored Markdown — heading structure, on-disk link resolution, readability, accessibility, terminology. Use when reviewing docs before publishing, wiring a docs CI gate, or auditing a Markdown corpus.

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Frontend development for React, Next.js, TypeScript, and Tailwind CSS. Use when building React components, optimizing Next.js performance, analyzing bundle sizes, scaffolding projects, implementing accessibility, or reviewing frontend code.

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Audit UI/UX designs for quality, AI-generated slop, and accessibility. Use when reviewing designs, detecting slop patterns, validating WCAG compliance, checking design-token adherence, or reviewing responsive design across breakpoints.

日本語の概要は準備中です。原文の説明を表示しています。