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概要と使いどころ
Reference and learning guide for the Model Context Protocol (MCP). Use when implementing clients/servers or debugging connection issues. For server listings, see mcp-server-registry.
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TLS 1.3 (RFC 8446) is the latest version of the Transport Layer Security protocol, providing significant improvements over TLS 1.2 in both security and performance. It reduces handshake latency to 1-R
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This skill covers deploying anomaly detection systems for industrial control environments using machine learning models trained on OT network baselines, physics-based process models, and behavioral analysis of industrial protocol communications. It addresses building normal behavior profiles for SCADA polling patterns, detecting deviations in Modbus/DNP3/OPC UA traffic, identifying rogue devices, and correlating network anomalies with physical process data from historians.
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Detects command-and-control (C2) communications tunneled through DNS protocol including DNS tunneling tools (Iodine, dnscat2, dns2tcp, Cobalt Strike DNS beacon), domain generation algorithms (DGA), encoded payload delivery via TXT/CNAME records, and DNS beaconing patterns. Covers Shannon entropy analysis of query subdomains, statistical anomaly detection, ML-based DGA classification, passive DNS correlation, and Zeek/Suricata signature development. Activates for requests involving DNS-based C2 detection, DNS tunnel identification, suspicious DNS traffic investigation, or DGA domain classification.
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Conducts authorized wireless network penetration tests to assess the security of WiFi infrastructure by testing for weak encryption protocols, captive portal bypasses, evil twin attacks, WPA2/WPA3 handshake capture, rogue access point detection, and client-side attacks. The tester evaluates wireless authentication, network segmentation, and the effectiveness of wireless intrusion detection systems. Activates for requests involving wireless pentest, WiFi security assessment, WPA2/WPA3 testing, or rogue access point detection.
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Detect and analyze covert communication channels used by malware including DNS tunneling, ICMP exfiltration, steganographic HTTP, and protocol abuse for C2 and data exfiltration.
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Analyzes network traffic generated by malware during sandbox execution or live incident response to identify C2 protocols, data exfiltration channels, payload downloads, and lateral movement patterns using Wireshark, Zeek, and Suricata. Activates for requests involving malware network analysis, C2 traffic decoding, malware PCAP analysis, or network-based malware detection.
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Captures and analyzes network packet data using Wireshark and tshark to identify malicious traffic patterns, diagnose protocol issues, extract artifacts, and support incident response investigations on authorized network segments.
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Guide for creating high-quality MCP (Model Context Protocol) servers that enable LLMs to interact with external services through well-designed tools. Use when building MCP servers to integrate external APIs or services, whether in Python (FastMCP) or Node/TypeScript (MCP SDK).
日本語の概要は準備中です。原文の説明を表示しています。
Guide for creating high-quality MCP (Model Context Protocol) servers that enable LLMs to interact with external services through well-designed tools. Use when building MCP servers to integrate external APIs or services, whether in Python (FastMCP) or Node/TypeScript (MCP SDK).
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Strategic clinical trial design feasibility assessment. Analyzes 6 dimensions (endpoint, population, comparator, effect size, duration, regulatory pathway) using precedent trials and FDA guidance. Produces enrollment projections, endpoint recommendations, and approval-pathway analysis. Use for trial-protocol design, power/sample-size estimation, comparator selection, and FDA submission strategy. Driven by precedent-based reasoning rather than first-principles math.
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Designs adaptive clinical trials including group-sequential (O'Brien-Fleming, Pocock, Lan-DeMets spending), sample-size re-estimation (blinded Friede-Kieser, unblinded Cui-Hung-Wang, Mehta-Pocock promising zone), seamless Phase 2/3 with treatment-arm selection, population enrichment, and response-adaptive randomisation. Covers FDA 2019 Final Adaptive Designs Guidance, FDA 2022 Master Protocols, and ICH E20 Step 2b/3 draft (June 2025, NOT final). Use when planning interim analyses, sample-size re-estimation, or master/platform-trial designs.
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Computes sample size and power for clinical trials including continuous, binary, and time-to-event endpoints; superiority, non-inferiority, and equivalence designs; FDA 2016 non-inferiority margin selection with M1/M2 framework; Schoenfeld 1981 and Lakatos 1988 for survival; Schuirmann TOST and 80-125% bioequivalence; minimum clinically important difference (MCID) vs δ distinction. Use when justifying trial size in protocol or SAP per CONSORT 2025 item 7.
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End-to-end CLIP-seq pipeline from FASTQ to ENCODE-compliant binding sites, single-nucleotide crosslink maps, annotation, motifs, and (optionally) differential binding. Use when running the full Yeo lab eCLIP / iCLIP / iCLIP2 / iCLIP3 / irCLIP / PAR-CLIP analysis with SMInput control, protocol-specific UMI extraction, ENCODE STAR parameters, CLIPper or Skipper peak calling with stringent log2 FC and -log10 p thresholds, IDR rescue and self-consistency QC, and downstream motif registration with mCross or PEKA.
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Agentic and machine-to-machine payments on Stellar. Covers x402 (HTTP 402 paid APIs via OZ Channels facilitator, fee-sponsored clients) and MPP (Machine Payments Protocol) in both Charge mode (per-request SAC) and Channel mode (off-chain commits, high-frequency). Defaults to USDC (SEP-41 SAC) on `stellar:testnet`/`stellar:pubnet` (CAIP-2). Use when selling a paid API to AI agents, building an x402 client, or designing a payment-channel architecture for high-frequency agent traffic.
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Stellar standards, ecosystem, and reference. Covers SEPs (Stellar Ecosystem Proposals), CAPs (Core Advancement Proposals), and a quick map for picking the right standard for wallets, anchors, payments, deposits/withdrawals, federation, deep links, and KYC. Also bundles ecosystem references (DeFi protocols, dev tools, wallets, infra, community projects), curated documentation links, and MCP servers (live tools such as Raven). Use when you need to know which SEP applies, or want a starting point for ecosystem integrations, official docs, or live MCP tooling.
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Send and receive cryptographically signed messages between AI agents using the Agent Messaging Protocol (AMP). Use when the user asks to "send a message to an agent", "check agent inbox", "message another agent", "reply to a message", "notify an agent", or any inter-agent communication task.
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A2A agent card JSON templates with schema validation and examples for different agent types. Use when creating agent cards, implementing A2A protocol discovery, setting up agent metadata, configuring authentication schemes, defining agent capabilities, or when user mentions agent card, agent discovery, A2A metadata, service endpoint configuration, or agent authentication setup.
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Expert blockchain developer specializing in smart contract development, DApp architecture, and DeFi protocols. Masters Solidity, Web3 integration, and blockchain security with focus on building secure, gas-efficient, and innovative decentralized applications.
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Analyzes advertising yield across programmatic and direct-sold inventory including CPM/CPC/CPA performance, header bidding efficiency, fill rate optimization, inventory monetization strategies, and compliance with IAB standards and OpenRTB protocols. USE THIS SKILL WHEN: - You need to audit ad revenue performance or diagnose yield drops - Someone asks about header bidding configuration or Prebid.js optimization - You are analyzing fill rates, eCPM trends, or ad unit performance - A project involves programmatic advertising, SSP integration, or ad serving - You need to verify ads.txt, sellers.json, or supply chain compliance - Someone mentions CPM floors, bid density, or auction dynamics - You are evaluating ad quality controls, viewability, or ad-related UX impact - A codebase integrates Google Ad Manager, AdMob, Prebid, or any SSP TRIGGER PHRASES: "ad yield", "eCPM", "fill rate", "header bidding", "Prebid", "programmatic ads", "ad monetization", "CPM optimization", "ad revenue", "ads.txt", "ad serving", "SSP", "ad unit performance", "viewability"
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Turns Hi-C/Micro-C FASTQ into a deduplicated, filtered .pairs file with pairtools and decides whether the library worked. Covers the bwa mem -SP5M / bwa-mem2 / chromap --preset hic alignment idiom (mates mapped as independent single-end reads), pairtools parse vs parse2 and the walks-policy choice (5unique pairwise vs all for Pore-C/Micro-C concatemers), pair-type classification (keep UU and rescued UC), dedup (PCR vs optical/by-tile), select by pair_type/MAPQ/distance, restriction-fragment handling (restrict, Arima dual-enzyme, Micro-C/DNase fragment-free), and allele-specific phasing (pairtools phase to two coolers). The library-QC decision uses % long-range cis as the one-number quality metric, trans as the noise floor, orientation balance as fragment-map-free dangling-end/self-circle QC, and % duplicates as a complexity proxy. Use when processing Hi-C/Micro-C/Omni-C reads into pairs, judging library quality, handling multi-enzyme or restriction-agnostic protocols, or generating allele-specific contacts.
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Analyzes single-cell pooled CRISPR screens (Perturb-seq, CROP-seq, Perturb-CITE-seq, ECCITE-seq, multiome) where each cell carries an sgRNA and a scRNA-seq / surface-protein / chromatin readout. Covers experimental design (direct-capture Perturb-seq Dixit 2016 vs CROP-seq 3'UTR-barcoded Datlinger 2017 vs ECCITE-seq vs Multiome), MOI for sgRNA assignment, escaper-cell filtering (Mixscape, Papalexi 2021), SCEPTRE NB GLM + permutation for low-MOI (Barry 2024 Genome Biol 25:124), the Pertpy framework, factor decomposition, genome-scale Perturb-seq (Replogle 2022 Cell, 2.5M cells), and per-perturbation single-cell DE. Use when running a single-cell CRISPR screen, choosing direct-capture vs CROP-seq architecture, filtering escaper cells, performing single-cell DE, integrating Perturb-seq with pathway analysis, scaling to GW CRISPRi via Replogle protocol, or analyzing multi-omics screens.
日本語の概要は準備中です。原文の説明を表示しています。
Computes sample size and power for clinical trials including continuous, binary, and time-to-event endpoints; superiority, non-inferiority, and equivalence designs; FDA 2016 non-inferiority margin selection with M1/M2 framework; Schoenfeld 1981 and Lakatos 1988 for survival; Schuirmann TOST and 80-125% bioequivalence; minimum clinically important difference (MCID) vs δ distinction. Use when justifying trial size in protocol or SAP per CONSORT 2025 item 7.
日本語の概要は準備中です。原文の説明を表示しています。
Preprocess CLIP-seq reads (eCLIP, iCLIP, iCLIP2, iCLIP3, irCLIP, PAR-CLIP, FLASH) with protocol-specific UMI extraction, adapter trimming, length filtering, and post-alignment PCR-duplicate collapse. Use when raw CLIP FASTQ must be turned into deduplicated, crosslink-preserving BAM input for peak calling; choosing between two-pass and single-pass adapter trimming; deciding minimum read length; or mapping UMI patterns to specific eCLIP/iCLIP/iCLIP2/iCLIP3 library preps.
日本語の概要は準備中です。原文の説明を表示しています。