「all」の検索結果

Submit or update a Wall of Apps entry in the App-Store-Connect-CLI repository using `asc apps wall submit`. Use when the user says "submit to wall of apps", "add my app to the wall", or "wall-of-apps".

日本語の概要は準備中です。原文の説明を表示しています。

Analyze current trends and challenges in Japanese NLP for a topic. Surveys the existing awesome-japanese-nlp-resources dataset and augments it with up-to-the-minute web research to produce a combined trend + issue report. Use only when the user explicitly wants a trend/landscape report, a challenges/limitations report, or a general research overview of a Japanese NLP topic (this combines the bundled dataset with live web research). Trigger phrases include '日本語LLMの最新トレンド', '〜の動向をまとめて', '最近の日本語NLPの流れ', '日本語LLMの課題', '〜の問題点・限界', '未解決の論点', 'trend report on Japanese embeddings', 'latest Japanese speech models', 'challenges in Japanese NER', 'limitations of Japanese embeddings'. For a simple lookup use the search skill; this one runs web research.

Expert knowledge for Azure DDoS Protection development including troubleshooting, best practices, decision making, architecture & design patterns, security, configuration, and integrations & coding patterns. Use when enabling DDoS IP/Network Protection, tuning policies, analyzing logs/metrics, or integrating NVAs and Azure Policy, and other Azure DDos Protection related development tasks. Not for Azure Firewall (use azure-firewall), Azure Firewall Manager (use azure-firewall-manager), Azure Web Application Firewall (use azure-web-application-firewall), Azure Virtual Network (use azure-virtual-network).

日本語の概要は準備中です。原文の説明を表示しています。

Install, update, version-check, or uninstall the Coco open-source AI workflow framework via bin/coco.js. Use when setting up Coco on a machine, checking out the pinned release tag into an existing clone, checking the installed version, or removing it. npx cocosuperintelligence is the published path (clones the pin and installs). Triggers on "install coco", "update coco", "set up coco", "coco cli", "uninstall coco".

日本語の概要は準備中です。原文の説明を表示しています。

Integrate ManagedCode.Orleans.Graph into an Orleans-based .NET application for grain-call policy enforcement, deadlock detection, live-call telemetry, and Mermaid graph diagnostics. USE FOR: ManagedCode.Orleans.Graph integration; allowed grain transitions; Orleans call filters; live policy graphs; reviewing Orleans call-cycle risk. DO NOT USE FOR: generic graph data modeling or traversal unrelated to Orleans calls. INVOKES: inspect the repository context, edit targeted files, and run relevant build, test, lint, or validation commands when changes are made.

日本語の概要は準備中です。原文の説明を表示しています。

Decision protocol for making side-effectful agent tools idempotent — so when an LLM tool call is retried (timeout, framework resume, user re-run, model duplicate emit), the second call is a no-op instead of a double-send. The load-bearing premise: the LM cannot promise it'll call exactly once; the tool must promise the second call is safe. Framework-agnostic — applies to LangGraph node bodies that re-run on resume, MCP tools, OpenAI tool-calling retries, CrewAI delegated tool invocations, and direct HTTP wrappers. Search keywords: duplicate email sent, charged twice, exactly-once, idempotency key, tool called twice, retry side effect, double-send, at-least-once delivery.

日本語の概要は準備中です。原文の説明を表示しています。

Report which kasetto-installed skills and MCP servers are actually being used across the AI agents on this machine, and render a branded HTML dashboard of the result. Use whenever the user asks what agent assets they actually use, which skills or MCPs are dead weight, what to prune or clean up from kasetto.yaml, why their context is bloated with unused MCP servers, whether a skill has ever been invoked, or wants a usage report, audit, or dashboard of their agent setup. Also trigger on "kst ai assets usage", "ai asset usage", "what skills do I actually use", "which MCPs are worth keeping", "audit my agent assets", "am I using all these skills", or any request to review, trim, or justify an installed agent toolkit.

日本語の概要は準備中です。原文の説明を表示しています。

Visualize copy number profiles, segments, allele-specific tracks, and cohort patterns from CNVkit, GATK, ASCAT, FACETS, Sequenza, and other callers. Covers genome-wide and per-chromosome log2 scatter plots, B-allele-frequency/minor-allele-fraction tracks, ideograms, cohort heatmaps, circos views, and caller-native plots. Use when creating publication CNV figures, choosing which plot answers a given question, diagnosing a wrong diploid baseline visually, displaying loss of heterozygosity, or deciding what depth-only plots cannot reveal.

日本語の概要は準備中です。原文の説明を表示しています。

Infer integer allele-specific copy number, tumor purity, and ploidy from tumor sequencing by jointly modeling read depth (logR) and B-allele frequency (BAF) with ASCAT, Sequenza, FACETS, PURPLE, and PureCN (tumor-only). Covers the purity-ploidy identifiability problem, the diploid-baseline (dipLogR) anchor, major/minor copy number, loss of heterozygosity, sunrise/contour fit diagnostics, and reconciliation of conflicting fits. Use when tumor analysis needs absolute copy number rather than relative log2, when estimating purity and ploidy, calling LOH or copy-neutral LOH, resolving whole-genome doubling, running tumor-only allele-specific calling, or choosing among ASCAT, Sequenza, FACETS, and PureCN.

日本語の概要は準備中です。原文の説明を表示しています。

音乐制作 / 混音 (音乐制作 / 混音 (Music Production & Mixing) — 录音棚与卧室工作室里把一首歌从编曲/录音/编程做到混音/母带成品的工程+艺术认知操作系统，面向制作人(producer)、混音师(mixing engineer)、母带工程师(mastering engineer)、beatmaker、唱作人/bedroom producer，以及想入行/服务这行(录音棚、plugin 厂商、sample 公司)的视角。覆盖: (a) 第一性张力 — **服务歌曲/情绪 (serve the song / emotion-first: 技术只为打动人，'if it sounds good it is good'，用耳朵不用眼睛) ⇄ 技术正确/工程纪律 (technical correctness: gain staging 增益结构、headroom 余量、相位 phase、监听校准、LUFS 响度规范)**; **黄金耳朵/直觉混音 (mix by ears, reference-driven, 不看分析仪) ⇄ 看表/分析仪/规则 (mix by metrics: EQ 曲线、频谱仪 spectrum analyzer、相关计 correlation meter)**; **少即是多/减法混音 (subtractive, 'less is more', 录好源头 = 混音成功一半) ⇄ 重度处理/'we'll fix it in the mix' (heavy processing, 后期补救)**; **In-the-box 全数字 (ITB: DAW + 插件，召回方便，bedroom 可达专业) ⇄ 模拟硬件/outboard (analog console + 硬件，'mojo'/谐波染色 vs 召回噩梦)**; **人性化/groove/不完美 (humanize, push/pull, 'the feel') ⇄ 量化/网格/修音 (quantize to grid, pitch-correct, 'in the pocket' 靠编辑)**; (b) 核心工作流 / pipeline (最标准、最易蒸高质量+CLI 化) — 编曲制作 arrangement/production → 录音/编程 tracking/programming → 编辑 editing (comping 选段/quantize/pitch-correct/time-align) → 混音 mixing (gain staging → balance 平衡 → EQ → compression → 空间 reverb/delay → 自动化 automation → 总线/母线处理 bus/mix-bus) → 母带 mastering (响度/EQ/限制 limiting/dither/排序+间隔) → 交付 delivery (DDP/streaming spec/stems); (c) 工具栈 — DAW (Pro Tools 录音棚标准/Ableton Live 电子+现场/Logic Pro Mac 性价比/FL Studio beatmaker/Cubase/Studio One/Reaper); 插件 (EQ: FabFilter Pro-Q/SSL/API; 压缩: 1176/LA-2A/SSL bus comp 及其复刻 Waves/UAD/Plugin Alliance; 混响: Valhalla/Lexicon/Bricasti; 调音: Auto-Tune/Melodyne; 限制器 limiter: Pro-L 2/Ozone); 硬件 (监听音箱 monitors: Yamaha NS-10/Genelec; 声学处理 acoustic treatment; 转换器 converters: Apollo/RME; 话筒 mics: U47/SM7B/57); 采样/音色 (Splice/sample packs/Kontakt/Serum 合成器); (d) 知识正典 — Mixing Secrets for the Small Studio (Mike Senior)、The Mixing Engineer's Handbook / Recording Engineer's Handbook (Bobby Owsinski)、Zen and the Art of Mixing (Mixerman)、The Art of Mixing (David Gibson 可视化)、Modern Recording Techniques、Sound on Sound 杂志、Mix With The Masters 大师课、Pensado's Place; (e) figures/流派 — 混音大师 (Chris Lord-Alge CLA 摇滚/Andrew Scheps 'Stadium' 3-bus/Tony Maserati/Manny Marroquin/Serban Ghenea 流行隐形混音/Michael Brauer multing); 制作人 (Rick Rubin 减法/氛围、Quincy Jones、Dr. Dre 完美主义、Max Martin 流行结构、Jack Antonoff、Finneas bedroom→Grammy、Metro Boomin/trap、Timbaland、Pharrell); 母带 (Bob Katz K-system/响度战争批判、Bob Ludwig、Greg Calbi); 教育者 (Mike Senior、Pensado、Warren Huart Produce Like A Pro、Dan Worrall 技术深挖); (f) 行业话术/黑话 — gain staging/headroom/LUFS/true peak/comping/bouncing/printing/stems/DI/reamp/sidechain/parallel compression 并行压缩/mid-side/bus/aux/send-return/dry-wet/transient/sibilance/de-ess/pumping/mud 浑浊/harsh 刺耳/boxy/muddy/in the pocket/the pocket/quantize/swing/groove/vibe/mojo/reference track/A/B/recall/print/glue 胶水; (g) 争议/批判 — 响度战争 loudness war (Bob Katz/Loudness Penalty/streaming 归一化 LUFS 终结了吗)、修音/Auto-Tune 之争 (artistic vs cheating)、ITB vs 模拟玄学 (硬件 'warmth' 是否安慰剂、null test 盲测)、preset/AI 混音 (iZotope/LANDR/sample-based 是否毁了 craft)、bedroom producer 民主化 vs 录音棚消亡、'loudness'/over-compression、plugin 复刻 vs 真硬件、Spotify normalization 对母带的影响、过度依赖 reference/抄袭; (h) 创意 vs 技术张力是本行业 deep tacit 知识的核心 — 大量隐性 '听感' 知识难以言传，靠 reference/学徒/千小时积累。不含: 现场扩声 live sound/PA、音乐理论/作曲 composition (虽相关但聚焦录音棚后期制作)、配乐/film scoring (虽相关但聚焦歌曲制作)、纯硬件电子工程/合成器 DIY、音乐商业/版权/发行 (虽相关但聚焦制作技艺本身)。) Master OS — automated mastery of 音乐制作 / 混音 (Music Production & Mixing) — 录音棚与卧室工作室里把一首歌从编曲/录音/编程做到混音/母带成品的工程+艺术认知操作系统，面向制作人(producer)、混音师(mixing engineer)、母带工程师(mastering engineer)、beatmaker、唱作人/bedroom producer，以及想入行/服务这行(录音棚、plugin 厂商、sample 公司)的视角。覆盖: (a) 第一性张力 — **服务歌曲/情绪 (serve the song / emotion-first: 技术只为打动人，'if it sounds good it is good'，用耳朵不用眼睛) ⇄ 技术正确/工程纪律 (technical correctness: gain staging 增益结构、headroom 余量、相位 phase、监听校准、LUFS 响度规范)**; **黄金耳朵/直觉混音 (mix by ears, reference-driven, 不看分析仪) ⇄ 看表/分析仪/规则 (mix by metrics: EQ 曲线、频谱仪 spectrum analyzer、相关计 correlation meter)**; **少即是多/减法混音 (subtractive, 'less is more', 录好源头 = 混音成功一半) ⇄ 重度处理/'we'll fix it i

日本語の概要は準備中です。原文の説明を表示しています。

B2B 大客户销售 (B2B 大客户销售 / 企业级复杂销售 (Enterprise B2B Sales / Complex Selling) — 面向企业客户的高价值、长周期、多干系人复杂销售的职业认知操作系统，从业者(AE/客户经理/SE 售前/销售负责人 VP Sales/CRO)、想入行者、以及服务这行(销售咨询/enablement/RevOps)的视角。覆盖: (a) 第一性张力 — 三大方法论流派之争: **买方主导的诊断式提问 (consultative/discovery-led: SPIN 提问 → 痛点 → 方案, 「像医生先诊断再开方」) ⇄ 卖方主导的教学式挑战 (Challenger: Teach-Tailor-Take control, 用商业洞见 reframe 客户认知, 「像老师挑战客户的既有想法」) ⇄ 资格审查纪律 (MEDDIC/MEDDPICC: 先严格 qualify/disqualify, 「no deal 好过 bad deal」)**; 三派不是非此即彼, 资深人混用, 但底层世界观分歧真实(问 vs 教 vs 筛); 更深层张力 — 「过程/方法论纪律 (process rigor: 预测准确、deal inspection、qualify) ⇄ 关系/信任 (relationship: champion 培养、高管关系、人情)」, 「卖结果/业务价值 (business outcome/ROI、卖给经济决策人) ⇄ 卖产品/功能 (feature selling)」, 「多线程触达买方委员会 (multithreading buying committee) ⇄ 单一 champion 依赖」; (b) 方法论正典 (最标准化、最易蒸出高质量+CLI 化) — MEDDIC/MEDDPICC (Metrics 量化指标/Economic buyer 经济决策人/Decision Criteria 决策标准/Decision Process 决策流程/Identify Pain 痛点/Champion 内部拥护者 + Paper process 签约流程/Competition 竞争), SPIN (Situation/Problem/Implication/Need-payoff 提问序列), Challenger (商业洞见 commercial insight + reframe + 建设性张力 constructive tension + Mobilizer 调动者), Solution Selling (痛点链 pain chain/愿景创建/buying vision), Sandler (前置合约 up-front contract/痛苦漏斗 pain funnel/潜艇七步), Command of the Message (Force Management: 业务价值框架/before-after/decision criteria), Gap Selling (Keenan: 现状 current state → 期望 future state → 差距 the gap/问题中心), Miller Heiman Strategic Selling (蓝表 blue sheet/buying influences: 经济/用户/技术买家 + coach), Value Selling, BANT (老式资格框架, 多被 MEDDIC 取代); (c) 行业结构与角色 — SDR/BDR (开发/约见) → AE/客户经理 (成交) → SE/售前/Solutions Engineer (技术验证/POC/demo) → CSM (客户成功/续约扩张) → Sales Manager/VP Sales/CRO; RevOps (收入运营), Sales Enablement (赋能); land and expand (先落地再扩张)、量化指标 quota/OTE(目标总收入)/配额达成率/pipeline coverage(管道覆盖率 ~3x 业内经验法则)/win rate(赢率)/sales velocity(销售速度)/ramp time(爬坡期)/ACV·ARR·NRR(年合同额/年经常性收入/净收入留存); (d) 销售周期 (the deal/opportunity lifecycle) — prospecting 开发 → discovery 需求挖掘 → qualification 资格审查(MEDDIC) → demo/technical validation/POC 技术验证 → business case/value 业务价值与 ROI → proposal 提案 → negotiation 谈判(procurement 采购/法务/security review) → close 成交 → onboarding/expansion 交付与扩张; 干系人: champion 拥护者 vs coach 线人 vs economic buyer 经济决策人 vs blocker 阻碍者; mutual action plan/close plan(双向行动计划/成交计划); (e) 产出物与节奏 — discovery call(需求电话)、demo、business case、proposal/SOW、MAP(mutual action plan)、forecast(预测: commit/best case/pipeline 类别)、pipeline review/deal review/QBR(季度业务回顾)/account plan(客户计划); (f) 职业/招聘/薪酬 — quota/OTE/comp plan(底薪+提成)/accelerator(加速器)/SPIFF/clawback、ramp、President's Club(顶尖销售俱乐部)、SDR→AE→管理 晋升路径、rep turnover(销售流失率高); (g) 争议/批判 — Challenger 是否过誉(CEB 原始数据被质疑、insight selling 批评)、MEDDIC 沦为填表剧场(box-checking theater, 填字段不真 qualify)、活动指标 vs 质量(spray-and-pray 群发/冷电话存废之争)、AI SDR/自动化淹没收件箱(2024-2025 AI BDR 泡沫 + 邮件送达率崩溃 backlash)、SDR 军团模式是否将死(Winning by Design/signal-based GTM 取代)、配额虚高与销售 burnout、季末打折促单、销售方法论的「宗教化」与培训-认证产业链、最大竞争对手其实是「no decision/维持现状」而非对手; (h) 流派/思想谱系 — 提问诊断派(Rackham SPIN/Bosworth&Eades Solution Selling/Keenan Gap Selling) vs 挑战教学派(Dixon&Adamson Challenger/Force Management Command of the Message) vs 资格纪律派(MEDDIC: Napoli&Dunkel 起源/John McMahon/MEDDICC Andy Whyte) vs 关系战略派(Miller Heiman Strategic/Large Account) vs SaaS 现代收入架构派(Aaron Ross Predictable Revenue/Mark Roberge Sales Acceleration Formula/Jacco van der Kooij Winning by Design bowtie/David Skok 指标) vs 开发-心态派(Jeb Blount Fanatical Prospecting/Sales EQ、Anthony Iannarino、Josh Braun) vs 谈判(Chris Voss Never Split the Difference 战术同理心)。不含: B2C/零售/电商导购销售、纯电话客服/客户支持、纯渠道/分销管理(channel 虽相关但聚焦直销复杂销售)、纯 marketing/需求生成(demand gen 虽上游但本 skill 聚焦销售执行)、纯 CSM 客户成功(虽相关但聚焦 new logo + expansion 的销售动作)。) Master OS — automated mastery of B2B 大客户销售 / 企业级复杂销售 (Enterprise B2B Sales / Complex Selling) — 面向企业客户的高价值、长周期、多干系人复杂销售的职业认知操作系统，从业者(AE/客户经理/SE 售前/销售负责人 VP Sales/CRO)、想入行者、以及服务这行(销售咨询/enablement/RevOps)的视角。覆盖: (a) 第一性张力 — 三大方法论流派之争: **买方主导的诊断式提问 (consultative/discovery-led: SPIN 提问 → 痛点 → 方案, 「像医生先诊断再开方」) ⇄ 卖方主导的教学式挑战 (Challenger: Teach-Tailor-Take control, 用商业洞见 reframe 客户认知, 「像老师挑战客户的既有想法」) ⇄

日本語の概要は準備中です。原文の説明を表示しています。

Suede AI MCP release QA, scoped to this pack's own server (mcp/suede-skills-mcp.mjs) and its catalog, install, and docs surface. Runs the full JSON-RPC lifecycle against a live server (initialize, notifications/initialized, ping, tools/list, tools/call, resources/list, resources/read, prompts/list, prompts/get), plus protocol negotiation, closed input and output schemas, tool annotations, structuredContent with text fallbacks, malformed-input probes, clean stdio, catalog-to-folder agreement, and install-path language. Use when the MCP server, mcp/catalog.json, a tool, resource, or prompt definition, or the MCP install docs change, or before publishing an MCP release. A check that did not run against the live server is a FAIL, not a skip. NOT FOR: a generic third-party MCP server, which this skill's hardcoded surface does not describe; fixing or testing the public install path itself (use suede-launch-packaging).

日本語の概要は準備中です。原文の説明を表示しています。

This skill should be used when the user asks to "create a challenge funnel", "build a 5-day challenge", "bootcamp funnel", "challenge launch", or mentions challenges, bootcamps, or multi-day engagement funnels. Creates challenge funnels that activate prospects, build community, and convert to core offers.

日本語の概要は準備中です。原文の説明を表示しています。

Install a Skill into the current workspace from a Git repository or local directory. Use this whenever the user wants to: - Add a new Skill to the agent ("install the X skill", "add the foo skill", "I want a skill that does Y, find one") - Pull the latest version of an already-installed Skill ("update the X skill", "refresh my skills") - List Skills currently active in the workspace - Remove a Skill they no longer need Trigger on phrases like "装一个 X skill", "安装技能", "install skill", "update skill", "list skills", "remove skill", "找个能 X 的 skill". Do not require the user to say the literal Skill name — if they describe a capability, propose a candidate Skill (Git URL or local path) and confirm before installing.

日本語の概要は準備中です。原文の説明を表示しています。

SwiftUI のレイアウト落とし穴・ベストプラクティス・非推奨パターンと、 iOS / watchOS アプリの実機配布 (Provisioning / App Group / Code Signing / Apple Watch Developer Mode / Xcode 15+ Debug dylib) トラブルシューティング のガイド。コード生成・修正時に既知のレイアウトバグを防ぎ、 App Group + watchOS + Apple Watch の実機インストール失敗を 7 段階フレームワークで診断・解消する。 Use when: SwiftUI のコードを書く・修正するとき。 レイアウト崩れを修正するとき。safeAreaInset や ViewThatFits を使うとき。 マルチデバイス対応するとき。iOS + watchOS アプリの実機ビルド / 配布で provisioning / App Group / Manual Signing / Apple Watch の UDID / Xcode 自動署名の 罠に詰まったとき。Apple Watch に "Could not install at this time" が出たとき。 Triggers: "SwiftUI", "layout", "safeAreaInset", "ViewThatFits", "GeometryReader", "レイアウト", "崩れ", "表示バグ", "iPhone SE", "ATT", "ATTrackingManager", "requestTrackingAuthorization", "AdMob", "広告", "Provisioning Profile", "App Group", "Manual Signing", "Code Signing", "Apple Watch", "watchOS", "Install できない", "Could not install at this time", "Bundle ID 紐付け", "Xcode Automatic Signing", "embedded.mobileprovision", "Spaceship", "App Store Connect API", "WKCompanionAppBundleIdentifier", "Developer Mode", "Privacy & Security", "watchOS Developer Mode", "Apple Watch に App を入れられない", "整合性を確認できなかった", "integrity check", "ENABLE_DEBUG_DYLIB", "__preview.dylib", "debug.dylib", "Xcode 15 Preview", "SwiftUI Preview dylib", "ENABLE_PREVIEWS", "AppIntents", "AppShortcut", "AppShortcutsProvider", "AppEntity", "AppEnum", "Siri", "Siri に流れる", "Siri がリマインダーに流れる", "updateAppShortcutParameters", "Invalid parameter type", "Invalid Utterance", "applicationName", "CFBundleDisplayName", "CFBundleSpokenName", "TaskEntityQuery", "AppEntityQuery", "TimelineProvider", "recommendations", "WatchConnectivity", "WCSession", "updateApplicationContext", "sessionDidBecomeInactive", "iPhone と Watch でデータ共有", "App Group 共有できない", "scenePhase", "ポーリング", "watchOS バッテリー", "TextField 文字色 watchOS", "Picker 文字色 watchOS", "Single Size AppIcon", "watchOS AppIcon"

Install skills, agents, and teams from agent-almanac into any supported agentic framework using the CLI. Covers framework detection, content search, installation with dependency resolution, health auditing, and manifest-based syncing. Use when setting up a new project with agentic capabilities, installing specific skills or entire domains, targeting multiple frameworks simultaneously, or maintaining a declarative manifest of installed content.

日本語の概要は準備中です。原文の説明を表示しています。

Use when using kmalloc, vmalloc, or the page allocator, sizing kmalloc allocations, working with SLUB or the buddy allocator, or debugging memory zones and kernel OOM.

日本語の概要は準備中です。原文の説明を表示しています。

The acceptance bar for anything Pulp can install (a `pulp tool` registry entry, `pulp add` package, or any downloadable). Validate the FULL lifecycle — install AND uninstall, from OUTSIDE a Pulp checkout, with the installed-user's binary — before its README or docs ship. TRIGGER when adding/editing tools/packages/tool-registry.json, a new binary_download/python_pip/npm_package tool, a `pulp add` importer, or writing docs that tell users to install something.

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Use when a task needs the judgment of a drywall and ceiling tile installer — specifying a fire-rated wall/ceiling assembly to a UL design number, hitting an STC target on a demising wall, choosing board type for a wet area, placing control joints on a long run, or diagnosing why a finished assembly failed inspection or a sound/mold complaint.

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Router for the 159 SfSkills `apex` skill packages. Apex and SOQL: triggers, Apex governor limits, async processing, OUTBOUND HTTP callouts, security enforcement, and test patterns. Owns calling an external API FROM Salesforce; salesforce-integration owns inbound. Generic nightly scheduling without naming code belongs to salesforce-flow. Codebase security review belongs to salesforce-security. NOT for SOSL — use salesforce-data. Use when the request mentions Apex, trigger, SOQL, Apex governor limit, batch, queueable, @future, schedulable, test class, CPU time, heap, with sharing, StripInaccessible, callout, HTTP callout, HttpRequest, call an external API, consume a REST API from Apex, Named Credential in Apex, HttpCalloutMock, Apex REST service. Finds and opens the exact skill package to read; it does not contain the guidance itself.

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Detects somatic mutations in circulating tumor DNA, treating low-VAF detection as a signal-versus-noise problem set by error suppression and molecules sampled, not by the choice of caller. Distinguishes de novo CALLING (scanning a panel for unknown variants, bounded by per-locus error and multiple testing) from tumor-informed DETECTION (tracking a pre-specified variant set, where panel integration reaches single-ppm). Covers VarDict and Mutect2 for de novo calling, UMI-aware callers, and a pysam-based known-variant VAF tracker, with matched-WBC subtraction as the mandatory defense against clonal hematopoiesis (the dominant false positive). Use when calling or tracking tumor mutations from plasma cfDNA, setting a VAF threshold, or deciding whether a low-VAF call is tumor versus CHIP.

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Infer integer allele-specific copy number, tumor purity, and ploidy from tumor sequencing by jointly modeling read depth (logR) and B-allele frequency (BAF) with ASCAT, Sequenza, FACETS, PURPLE, and PureCN (tumor-only). Covers the purity-ploidy identifiability problem, the diploid-baseline (dipLogR) anchor, major/minor copy number, loss of heterozygosity, sunrise/contour fit diagnostics, and reconciliation of conflicting fits. Use when tumor analysis needs absolute copy number rather than relative log2, when estimating purity and ploidy, calling LOH or copy-neutral LOH, resolving whole-genome doubling, running tumor-only allele-specific calling, or choosing among ASCAT, Sequenza, FACETS, and PureCN.

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Visualize copy number profiles, segments, allele-specific tracks, and cohort patterns from CNVkit, GATK, ASCAT, FACETS, Sequenza, and other callers. Covers genome-wide and per-chromosome log2 scatter plots, B-allele-frequency/minor-allele-fraction tracks, ideograms, cohort heatmaps, circos views, and caller-native plots. Use when creating publication CNV figures, choosing which plot answers a given question, diagnosing a wrong diploid baseline visually, displaying loss of heterozygosity, or deciding what depth-only plots cannot reveal.

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Build call automation workflows with Azure Communication Services Call Automation Java SDK. Use when implementing IVR systems, call routing, call recording, DTMF recognition, text-to-speech, or AI-powered call flows.

日本語の概要は準備中です。原文の説明を表示しています。