「alternatives」の検索結果

Designs donor/repair templates for precise CRISPR knock-ins -- choosing the format (ssODN, long-ssDNA/Easi-CRISPR, dsDNA/plasmid, AAV6), sizing homology arms, placing the cut within ~10 bp of the edit, and adding a mandatory codon-checked blocking (PAM/seed) mutation so the edited allele is not re-cut. Frames the HDR-vs-NHEJ-vs-MMEJ pathway competition, the MMEJ (PITCh) and homology-independent (HITI/HMEJ) alternatives for post-mitotic cells, ssODN strand/asymmetry choice, phosphorothioate end-protection, and ranked HDR enhancers. Use when designing a donor for a point mutation, epitope/fluorophore tag, allele replacement, or knock-in, or when HDR efficiency is low. Guide design and base/prime editing are separate skills.

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Differential abundance (DA) and differential state (DS) analysis for flow and mass cytometry - tests which cell populations change in frequency or marker expression between conditions using diffcyt (edgeR/voom/GLMM for DA, limma/LMM for DS), with cydar, CITRUS, and compositional methods (sccomp, scCODA, DCATS) as alternatives. Covers the sample-is-the-experimental-unit principle, design/contrast and mixed-model formulas, compositionality of cluster proportions, and FDR across clusters. Use when comparing populations between groups, choosing a DA method, handling paired/batch designs, or deciding whether compositional correction is needed.

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Corrects the gene-independent copy-number artifact in CRISPR-Cas9 screens (Aguirre 2016 / Munoz 2016 Cancer Discov) where amplified loci appear essential from DNA-damage burden of simultaneous cuts. Covers the gene-independent DNA-damage / G2-arrest mechanism, CRISPRcleanR (Iorio 2018) unsupervised pre-hoc correction, CERES (Meyers 2017) joint CN + gene-effect model, Chronos (Dempster 2021) DepMap-standard population-dynamics + CN model with lowest residual bias, the decision tree by data availability, the Spearman LFC-vs-CN diagnostic, focal-amplification examples (ERBB2 in HER2+, MYC in colorectal, FGFR1 in head and neck), and CRISPRi/a alternatives that bypass the artifact. Use when screening cancer cell lines, diagnosing essentiality at amplified loci, choosing CRISPRcleanR / CERES / Chronos, deciding whether CN correction is needed before MAGeCK / BAGEL2 / drugZ, or switching from Cas9 to CRISPRi.

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Performs alchemical free-energy calculations including relative binding free energy (RBFE / FEP+) and absolute binding free energy (ABFE) via OpenFE, FEP+, GROMACS, AMBER pmemd, and OpenMM with explicit lambda scheduling, soft-core potentials, MBAR/BAR analysis, cycle-closure validation, and protocol-appropriate enhanced sampling. Compares ML alternatives (Boltz-2 affinity, DeepDock). Use when ranking analogs by binding affinity beyond docking accuracy, performing prospective lead optimization, or validating SAR predictions.

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Market positioning strategy using the April Dunford framework, enriched with JTBD discovery, Moore positioning statement, and Neumeier's Onliness Test. Produces a complete positioning document, positioning statement, competitive alternatives map, and market category analysis. Use when the user wants to define or refine their market positioning, find their unique position, differentiate from competitors, craft a positioning statement, choose a market category, or figure out "how should we position this product." Triggers for "positioning", "how to position", "market position", "differentiation strategy", "positioning statement", "competitive positioning", "category strategy", "where do we fit in the market", "how are we different", "unique value proposition", or any request to define, sharpen, or rethink positioning. Works standalone — no prior startup-design or startup-competitors session needed, but leverages their output if available.

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React useEffect best practices from official docs. Use when writing/reviewing useEffect, useState for derived values, data fetching, or state synchronization. Teaches when NOT to use Effect and better alternatives.

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After Effects animation rendering for web and React applications. Use this skill when implementing Lottie animations, JSON vector animations, interactive animated icons, micro-interactions, or loading animations. Triggers on tasks involving Lottie, lottie-web, lottie-react, dotLottie, After Effects JSON export, bodymovin, animated SVG alternatives, or designer-created animations. Complements GSAP ScrollTrigger and Framer Motion for scroll-driven and interactive animations.

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Evaluate, select, and contract with vendors and SaaS tools. Use this skill when comparing alternatives, running an RFP, scoring vendors against criteria, negotiating contracts, planning a switch, or assessing a vendor's risk. Triggers on vendor evaluation, RFP, vendor selection, build vs buy, SaaS evaluation, vendor scorecard, vendor comparison, contract negotiation, vendor switch, procurement. Also triggers when a renewal is coming up or when a tool isn't meeting expectations.

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YC Office Hours — product idea diagnostic before writing code. Two modes: Startup (6 forcing questions with rigorous pushback) and Builder (generative brainstorming). Outputs design doc. Includes landscape research, premise challenge, alternatives generation, visual wireframe sketching, and optional cross-model second opinion.

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Prevent 此地无银三百两式 residue: finalize artifacts without echoing rejected session-only alternatives into labels, metadata, commits, PRs, or handoffs. Use after corrections or discarded proposals. Not for ordinary deletion, deprecation, migration, or exclusions materially required for safety, accuracy, compatibility, audit, quotation, or requested comparison.

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Create competitor comparison and alternative pages for SEO and sales enablement. Use when building alternative pages, vs pages, or competitor-vs-competitor pages, planning comparison content, or managing competitor data.

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Build competitor comparison pages: X-vs-Y, alternatives and roundups, with schema. "create a vs page"

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When the user wants to create competitor comparison or alternative pages for SEO and sales enablement. Also use when the user mentions 'alternative page,' 'vs page,' 'competitor comparison,' 'comparison page,' '[Product] vs [Product],' '[Product] alternative,' 'competitive landing pages,' 'how do we compare to X,' 'battle card,' or 'competitor teardown.' Use this for any content that positions your product against competitors. Covers four formats: singular alternative, plural alternatives, you vs competitor, and competitor vs competitor. For sales-specific competitor docs, see sales-enablement.

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Design game concepts from a novel. From SOURCE_BIBLE and PRODUCT_BRIEF, compare meaningful alternatives on the dimensions still unlocked, then select a playable direction using hard vetoes and explicit trade-offs. Use for what game should this novel become, compare game concepts, choose a game direction for this book, decide whether this novel should become an interactive story. 小说游戏概念设计。根据 SOURCE_BIBLE 与 PRODUCT_BRIEF 比较未锁定维度上的有效替代方案，用硬否决和关键取舍选择可玩方向。用于判断小说适合做成什么游戏、该不该做成互动叙事、比较游戏方案等需求。

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Design a wise systems intervention from an existing analysis. Maps proposed actions against Meadows' leverage points, checks for unintended consequences, and generates alternatives.

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Generates multiple means of engagement, representation, and action/expression for a given learning goal. Produces specific, practical alternatives — not generic options — and recommends the highest-impact single change.

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When the user wants to create sales collateral, pitch decks, one-pagers, objection handling docs, or demo scripts. Also use when the user mentions 'sales deck,' 'pitch deck,' 'one-pager,' 'leave-behind,' 'objection handling,' 'ROI calculator,' 'demo script,' 'talk track,' 'sales playbook,' 'proposal template,' or 'buyer persona card.' For competitor battle cards and comparison pages, see competitor-alternatives. For marketing website copy, see copywriting. For cold outreach emails, see cold-email.

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Swift 6.2 InlineArray and Span types for zero-overhead memory access, fixed-size collections, and safe pointer alternatives. Use when optimizing performance-critical code paths.

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Adds Smartlead-compatible spintax to cold email sequences for deliverability. Use this skill whenever the user asks to add spintax, spin text, add variations, or improve deliverability of email copy — especially when they mention Smartlead, cold email sequences, or paste HTML email content. Also trigger when the user says "spintax", "spin", "variations", "deliverability spintax", or references adding randomized alternatives to email copy. Works with both plain text and HTML input.

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April Dunford's 10-step positioning methodology for B2B tech products. Use when customers don't understand the product, sales cycles are long because reps must "explain it," prospects compare you to wrong competitors, or you face price pressure despite a good product. Covers competitive alternatives, unique attributes, value themes, target market characteristics, market category (Head to Head / Big Fish Small Pond / New Game), and trend layering.

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Generate A/B test variants for affiliate content. Triggers on: "create A/B test", "test my headline", "optimize my CTA", "generate variants", "split test ideas", "improve click-through rate", "test my landing page copy", "headline alternatives", "CTA variations", "which version is better", "optimize conversions", "test my email subject line", "compare approaches".

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Write "Top N best..." listicle articles for affiliate marketing with mini-reviews, pricing, pros/cons, and CTAs per entry. Triggers on: "write a best of list", "top 10 [category] tools", "best [product category] article", "roundup post", "listicle about [category]", "write a top tools article", "best [N] alternatives to [product]", "product roundup", "write a tools comparison list", "best software for [use case]", "top picks for [category]".

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Audit and tend to existing travel reservations. Reads a user-provided reservations file, then systematically checks every booking for price drops, better cabin availability, improved routings, schedule changes, nearby airport alternatives, and award repricing opportunities. Invoke proactively on a regular cadence or when the user says "garden", "check my bookings", "audit reservations", "price drop", "better routing", "did my flight change", "tend reservations", "reservation maintenance", or "check my trips".

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Search Amex travel portal for cash prices, MR points pricing, IAP discounts, and FHR/THC hotel benefits via Patchright. Use when comparing pay-with-points portal pricing to award alternatives.

日本語の概要は準備中です。原文の説明を表示しています。