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Map N6-methyladenosine (m6A) RNA modifications at single-nucleotide resolution using miCLIP (Linder 2015), miCLIP2 + m6Aboost machine learning (Kortel 2021), GLORI (Liu 2023, antibody-free chemical conversion), DART-seq (Meyer 2019, APOBEC1-YTH fusion), m6Anet (nanopore direct RNA), or MeRIP-seq with calibration. Use when distinguishing antibody-based from antibody-free m6A detection methods, applying the DRACH motif constraint, reconciling cross-method disagreements (DART 44% in DRACH vs GLORI), or detecting m6Am at the cap.

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Organize a Power BI or Analysis Services semantic model end to end so it is ready for report authors, Excel users and Copilot - table groups, display folders, Title Case business names and naming conventions, hierarchies, sort-by columns, hiding, Analyze in Excel (IsAvailableInMDX), descriptions, format strings, data categories, context annotations, DAX formatting and UDFs, calculated-object justification, duplicate removal, perspectives, translations and security roles. Use whenever the user asks to "organize", "tidy", "clean up", "polish", "prepare", "standardize" or "make report-ready" a model or one table in it (for example "organize the date table"), to "fix naming conventions", "rename fields", "make names human readable" or "fix abbreviations", even when they name only one of these aspects, and whenever a model is driven through the Tabular Editor 3 MCP or the te CLI for that purpose. Not for DAX performance tuning (dax skill) or model design and star-schema changes (semantic-model skill).

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MHC (major histocompatibility complex) molecules are cell surface complexes that present antigens to T cells. The repertoire of antigens presented in a given genetic background largely depends on the sequence of the encoded MHC molecules, and thus, in humans, on the highly variable HLA (human leukocyte antigen) genes of the hyperpolymorphic HLA locus. More than 28,000 different HLA alleles have been reported, with significant differences in allele frequencies between human populations worldwide

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Quarantines an intermittently failing test behind a ticket with an owner and expiry, assigns a root-cause bucket from isolated reruns, and defines when the test leaves quarantine. Use when a test is a QUARANTINE_CANDIDATE or fails without a code change; not for deterministic failures.

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Canonical patterns for writing custom MSBuild targets. USE FOR: diagnosing and fixing custom target authoring anti-patterns; broken SDK target chains across files (e.g., Directory.Build.targets silently redefining SDK targets); targets that replace CompileDependsOn instead of extending it with $(CompileDependsOn); query targets returning stale results from Outputs vs Returns misuse; missing Inputs/Outputs causing unnecessary rebuilds; missing FileWrites registration. Covers DependsOnTargets vs BeforeTargets vs AfterTargets, the Build→CoreBuild three-level pattern, and the $(XxxDependsOn) chain-extension pattern. DO NOT USE FOR: incremental build tuning (use incremental-build), parallelization (use build-parallelism), general anti-patterns (use msbuild-antipatterns), non-MSBuild build systems.

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Build structured semantic layer documentation for metrics, dimensions, and entities. Activate when you need to define a business metric, document a data model, or create YAML definitions compatible with dbt Semantic Layer or similar frameworks.

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This skill should be used when refactoring existing CSS from inline styles or utility classes to semantic patterns. Triggers on "refactor CSS", "extract styles", "consolidate CSS", "convert inline", "clean up styles", "migrate to semantic". Transforms to semantic classes with dark mode and tests.

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Ultra-fast RNA-seq transcript/gene quantification via quasi-mapping (no BAM). Builds a k-mer index from transcriptome FASTA, quantifies in minutes. Outputs TPM/count tables (quant.sf) with optional GC- and sequence-bias correction. Integrates with tximeta/tximport for DESeq2/edgeR. Use STAR when a genome-aligned BAM is needed.

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Match BIM quantities to CWICR work items. Map element categories to cost codes, validate quantities, and generate cost-linked QTOs.

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Use bitsandbytes for k-bit PyTorch quantization, Hugging Face quantized model loading, 8-bit and paged optimizers, direct quantized layers/functions, and backend installation diagnostics.

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Configure and orchestrate parallel agents using the standalone Antigravity 2.0 Agent Manager and Antigravity IDE.

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Eliminate sycophantic agreement patterns in AI responses. Load via /skill anti-sycophancy.

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Guides assessment and use of the EU-US Data Privacy Framework adequacy decision for transatlantic data transfers. Covers DPF self-certification with the Department of Commerce, DPF principles compliance, Data Protection Review Court, and annual EC review. Keywords: DPF, EU-US, adequacy, Privacy Shield, transatlantic transfers.

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Analyzes and verifies semantic equivalence between two functions, classes, or modules by examining control flow, data flow, and observable behavior. Use when comparing code implementations (refactored vs original, different implementations of same functionality, migration verification), determining if two code artifacts produce identical behavior, identifying behavioral differences between code versions, or validating that code changes preserve semantics. Supports formal reasoning and symbolic execution approaches.

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Identify bug-introducing commits using semantic analysis that extends traditional SZZ algorithm. Distinguishes semantic changes from refactorings or code movements using control-flow and data-flow similarity analysis. Use when analyzing bug-fix commits to trace back to bug-introducing changes, investigating software evolution, conducting empirical studies on defect prediction, or reducing false positives in bug localization. Supports git repositories and provides explanations for why commits are identified as bug-introducing.

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Think and work like an expert Antimicrobial Resistance Scientist. Use when a task calls for Antimicrobial Resistance Scientist judgment. Reasons from MIC distributions, ECOFFs, and phenotype–genotype concordance through EUCAST/CLSI AST, CARD/RGI and AMRFinderPlus resistomes, GLASS/WHONET/NARMS surveillance, carbapenemase/ESBL/MRSA mechanism panels, and heteroresistance PAP while treating breakpoint mixing, genotype-without-phenotype overclaim, selection-bias surveillance, and CARD version drift as first-class failure modes.

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Predict B-cell and T-cell epitopes using BepiPred, IEDB tools, and structure-based methods for vaccine and antibody design. Identify immunogenic regions in antigens. Use when designing vaccines, mapping antibody binding sites, or predicting immunogenic peptides.

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Quantifies CRISPR editing outcomes with CRISPResso2 (Clement 2019 Nat Biotechnol) across Cas9-nuclease (indels, HDR), CBE and ABE base editors (target conversion + bystander), and prime editor (pegRNA-templated) modes. Covers single-amplicon (CRISPResso), multi-sample batch (CRISPRessoBatch), pooled-amplicon (CRISPRessoPooled), WGS off-target (CRISPRessoWGS), and sample-comparison (CRISPRessoCompare) workflows; quantification-window math that controls what is called edited; substitution-vs-indel diagnostic to distinguish BE from Cas9 contamination; MMEJ deletion pattern interpretation; allele-frequency tables; and failure modes from amplicon misalignment or contamination. Use when quantifying editing from amplicon sequencing, choosing CRISPResso mode by design, distinguishing intended edits from bystanders and indel byproducts, debugging low-alignment runs, or generating publication-grade editing reports.

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Quantify transcript expression using pseudo-alignment with Salmon or kallisto. Use when quantifying transcripts with Salmon or kallisto.

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Build production-ready AI agents with PydanticAI — type-safe tool use, structured outputs, dependency injection, and multi-model support.

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生成一份对外可分享、脱敏的 AI-Native 开发者 README。 量化展示我对 Claude Code + Codex CLI + Kiro (AWS) + Trae (ByteDance) + Gemini Antigravity (Google) + Cursor 的使用深度、AI 协作风格、 项目与领域分布、兴趣主题，以及与 GitHub 提交的产出关联。 Trigger when the user says: "生成我的 AI 档案" / "做一份 AI-native README" / "分析我的 Claude / Codex / Kiro / Trae / Antigravity / Cursor 使用情况" / "总结我的 AI 使用" / "生成 AI 月度报告" / "按月份分析我的 AI 编码" / "分析 2026-05 的 AI 使用" / "build my AI usage profile" / "build my monthly AI coding report" / "analyze my AI usage for May 2026" / "summarize my Claude / Codex / Kiro / Trae / Antigravity / Cursor history" / "生成开发者画像". 全程本地、只读、默认匿名、不上传任何数据。

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Assists with storing, searching, and managing vector embeddings using ChromaDB. Use when building RAG pipelines, semantic search engines, or recommendation systems. Trigger words: chromadb, chroma, vector database, embeddings, semantic search, similarity search, vector store, rag.

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Design and implement Monte Carlo methods for uncertainty quantification, risk analysis, and probabilistic simulations across scientific and financial domains. Use when "monte carlo, random sampling, uncertainty quantification, risk analysis, stochastic simulation, MCMC, variance reduction, probabilistic, " mentioned.

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Accessibility isn't a feature - it's a fundamental quality of good software. 1 in 4 adults has a disability. Many more have temporary impairments or situational limitations. Your users include people who can't see, can't hear, can't use a mouse, or can't process information the way you do. This skill covers WCAG guidelines, ARIA patterns, keyboard navigation, screen reader compatibility, and automated testing. Key insight: accessible code is better code. The constraints of accessibility lead to simpler, more semantic, more maintainable implementations. 2025 lesson: Accessibility lawsuits are at an all-time high. "We'll add it later" is both ethically wrong and legally risky. Start accessible, stay accessible. Use when "accessibility, a11y, wcag, aria, screen reader, keyboard navigation, focus trap, alt text, color contrast, skip link, accessible, accessibility, a11y, wcag, aria, screen-reader, keyboard, inclusive, semantic-html" mentioned.

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