1.4万 件 ・ 関連度順
概要と使いどころ
Trim multiple sequence alignments using ClipKIT, trimAl, BMGE, Divvier, or HMMcleaner with mode selection guidance per downstream goal. Use when removing unreliable columns or contaminating residues before phylogenetic inference, HMM building, or selection analysis.
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Detects differential alternative splicing between conditions using rMATS-turbo (binomial LRT on junction counts), leafcutter (Dirichlet-multinomial GLM on intron clusters), MAJIQ V3 deltapsi/HET (Bayesian posterior on LSVs), SUPPA2 (empirical-null on TPM-derived PSI), or Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage). Reports FDR-corrected significance and delta PSI effect sizes. Tools differ in statistical model, annotation dependence, calibration regime, and replicate-count requirements. Use when comparing splicing patterns between treatment groups, tissues, or disease states.
日本語の概要は準備中です。原文の説明を表示しています。
Analyzes alternative splicing from PacBio Iso-Seq (HiFi, Kinnex/MAS-Iso-seq) and Oxford Nanopore (direct cDNA, direct RNA, R10.4.1+) long-read RNA-seq with full-isoform resolution. Tools include FLAIR (correct/collapse/quantify/diffSplice for PacBio + ONT), IsoQuant (de-novo or annotation-guided isoform discovery 2024 SOTA), Bambu (annotation-aware Bayesian discovery + quantification with Novel Discovery Rate), SQANTI3/SQANTI-LR (isoform classification: FSM/ISM/NIC/NNC + artifact flags), rMATS-long (event calling on long-read isoforms), and minimap2 (-ax splice:hq for HiFi; -ax splice -k14 for ONT cDNA; add -uf only for direct RNA or stranded cDNA preps). Solves microexon detection, recursive splicing, complex multi-exon isoforms, and DTU without transcript-quantification uncertainty. Use when short-read AS limitations (anchor length, complex isoforms, microexons, recursive splicing, transcript ambiguity) demand full-isoform resolution.
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Test whether two or more traits share a causal variant at a locus using Bayesian colocalization (coloc.abf, coloc.susie, HyPrColoc, moloc, eCAVIAR, SMR/HEIDI, PWCoCo, SharePro). Use when integrating GWAS with eQTL/sQTL/pQTL/mQTL, distinguishing shared causal variants from LD-driven coincidence, handling allelic heterogeneity, choosing between single-causal vs multi-causal methods, picking PP.H4 thresholds, running sensitivity over p12, or harmonising summary statistics for colocalization.
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Design durable workflows with Temporal for distributed systems. Covers workflow vs activity separation, saga patterns, state management, and determinism constraints. Use when building long-running processes, distributed transactions, or microservice orchestration.
日本語の概要は準備中です。原文の説明を表示しています。
Run the advanced iterative workflow — Wayfinding AST discovery, Relentless Grilling, contract and spec design, TDD, council review, and docs.
日本語の概要は準備中です。原文の説明を表示しています。
Provides REST API design standards and best practices for Spring Boot projects. Use when creating or reviewing REST endpoints, DTOs, error handling, pagination, security headers, HATEOAS and architecture patterns.
日本語の概要は準備中です。原文の説明を表示しています。
Spring Boot architecture patterns, REST API design, layered services, data access, caching, async processing, and logging. Use for Java Spring Boot backend work.
日本語の概要は準備中です。原文の説明を表示しています。
Provides expert knowledge of the testing pyramid, test design principles, MockK patterns, and integration testing conventions for Spring Boot + Kotlin projects.
日本語の概要は準備中です。原文の説明を表示しています。
Provides expert knowledge of the testing pyramid, test design principles, MockK patterns, and integration testing conventions for Spring Boot + Kotlin projects.
日本語の概要は準備中です。原文の説明を表示しています。
Design and coordinate multi-agent systems where specialized agents work together to solve complex problems. Covers agent communication, task delegation, workflow orchestration, and result aggregation. Use when building coordinated agent teams, complex workflows, or systems requiring specialized expertise across domains.
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Generates Spring Boot 3.x configurations, creates REST controllers, implements Spring Security 6 authentication flows, sets up Spring Data JPA repositories, and configures reactive WebFlux endpoints. Use when building Spring Boot 3.x applications, microservices, or reactive Java applications; invoke for Spring Data JPA, Spring Security 6, WebFlux, Spring Cloud integration, Java REST API design, or Microservices Java architecture.
日本語の概要は準備中です。原文の説明を表示しています。
JPA/Hibernate patterns for entity design, relationships, query optimization, transactions, auditing, indexing, pagination, and pooling in Spring Boot.
日本語の概要は準備中です。原文の説明を表示しています。
Improve detections, hardening, and response for the estate you defend. Detection-as-code and control design, not offense.
日本語の概要は準備中です。原文の説明を表示しています。
Build and configure Laravel 10+ applications, including creating Eloquent models and relationships, implementing Sanctum authentication, configuring Horizon queues, designing RESTful APIs with API resources, and building reactive interfaces with Livewire. Use when creating Laravel models, setting up queue workers, implementing Sanctum auth flows, building Livewire components, optimising Eloquent queries, or writing Pest/PHPUnit tests for Laravel features.
日本語の概要は準備中です。原文の説明を表示しています。
Map controls to SOC2, ISO 27001, PCI, or internal policy for the org you work for. Evidence and control design — not audit theater.
日本語の概要は準備中です。原文の説明を表示しています。
Provides expert knowledge of JPA entity design, relationship mapping, Flyway migrations, and PostgreSQL-specific optimizations.
日本語の概要は準備中です。原文の説明を表示しています。
Harden network architecture, segmentation, TLS, and allowlists for systems you operate. Defensive design and review only.
日本語の概要は準備中です。原文の説明を表示しています。
Provides dependency injection patterns for Spring Boot projects, including constructor-first design, optional collaborator handling, bean selection, and wiring validation. Use when creating services and configurations, replacing field injection, or troubleshooting ambiguous or fragile Spring wiring.
日本語の概要は準備中です。原文の説明を表示しています。
Hunt for adversary techniques in telemetry you are authorized to query. Hypothesis-driven, evidence-first, no live offensive operations.
日本語の概要は準備中です。原文の説明を表示しています。
Use when working with domain models, aggregates, value objects, domain events, or repositories in a DDD-style project. Ensures rich domain model over anemic CRUD.
日本語の概要は準備中です。原文の説明を表示しています。
Run the full feature workflow — task validation, technical design, implementation, testing, review, and documentation.
日本語の概要は準備中です。原文の説明を表示しています。
Provides expert knowledge for designing, implementing, and managing REST API versioning strategies with deprecation workflows.
日本語の概要は準備中です。原文の説明を表示しています。
Provides expert knowledge for building modern native Android apps using Kotlin, Jetpack Compose, Material Design 3, MVI architecture, Hilt dependency injection, and Media3/ExoPlayer.
日本語の概要は準備中です。原文の説明を表示しています。