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概要と使いどころ
This package implements sampling, iteration, and input of FASTQ files. The package includes functions for filtering and trimming reads, and for generating a quality assessment report. Data are represented as DNAStringSet-derived objects, an
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MSA2dist calculates pairwise distances between all sequences of a DNAStringSet or a AAStringSet using a custom score matrix and conducts codon based analysis. It uses scoring matrices to be used in these pairwise distance calcualtions which can be adapted to any scoring for DNA or AA characters. E.g. by using literal distances MSA2dist calculates pairwise IUPAC distances.
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Seamlessly interfaces the Basic Local Alignment Search Tool (BLAST) to search genetic sequence data bases. This work was partially supported by grant no. R21HG005912 from the National Human Genome Research Institute.
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Expert knowledge for Azure Nutanix development including decision making. Use when selecting NC2 on Azure regions, VM SKUs, and planning capacity, performance, availability, or cost, and other Azure Nutanix related development tasks. Not for Azure VMware Solution (use azure-vmware-solution), Azure Baremetal Infrastructure (use azure-baremetal-infrastructure), Azure Virtual Machines (use azure-virtual-machines), Azure Stack Edge (use azure-stack-edge).
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Expert knowledge for Azure Large Instances development including troubleshooting, limits & quotas, and integrations & coding patterns. Use when configuring Epic SKUs, sizing volume groups, tuning EHR storage, or resolving Epic–ALI connectivity/perf issues, and other Azure Large Instances related development tasks. Not for Azure Baremetal Infrastructure (use azure-baremetal-infrastructure), Azure Virtual Machines (use azure-virtual-machines), Azure Virtual Machine Scale Sets (use azure-vm-scalesets), Azure HPC Cache (use azure-hpc-cache).
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Cross-platform social research - narrative-first intelligence on what people are saying about a topic across Reddit, X, HN, Polymarket, and the web over the last 30 days
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Amazon negative keyword management and optimization. Search term analysis, waste reduction, savings estimation, and automated negative keyword strategies. Use when the user asks about negative keywords, search term analysis, ad waste reduction, or PPC optimization.
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Plan and execute substantial multi-file builds, features, migrations, and refactors with Astra as orchestrator and DeepSeek V4.1 Flash as the native Codex implementation worker. Use for phased planning and delegated build execution, including existing Superpowers or GSD plans. Skip trivial edits and explicitly single-agent tasks. Worker children must not invoke this orchestration skill.
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Seedance 2.5 prompt director — the omni-reference dialect. Routes the four generation modes (t2v / omni_reference / video_edit / video_extension), writes explicit @Image/@Video/@Audio reference roles with exclusions, stages 30-second videos into end-state beats, and covers video editing, forward/backward extension, first-last-frame and multi-keyframe control, storyboard grids, blockout rendering, and seamless transitions. Use whenever the user asks for a Seedance 2.5 prompt, mentions Seedance 2.5 / Dreamina / Jimeng, wants a clip longer than 15s on Seedance, wants to EDIT or EXTEND an existing video rather than generate a new one, or supplies more than a handful of image/video/audio references. For Seedance 2.0 (4K, `mode=fast`, or a genre hint) use higgsfield-seedance instead.
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Use when the user wants to create a complete multi-shot video, asks how to chain Higgsfield tools together, wants to build a short film or branded content series, asks "what's the full workflow", needs to connect Popcorn → image → video → Recast → audio → assembly, or wants to understand how the platform works as a production system rather than isolated tools.
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查询 willcodexquotareset.com 的 Codex 配额重置预测，返回 forecast 中的重置概率（score 百分比）、距上次重置的天数与小时数、最近重置时间、是否已宣布重置和评分构成。当用户询问 Codex quota 是否会重置、重置概率、上次重置多久、Will Codex Quota Reset 网站预测或要求调用 /api/forecast 时使用；不用于查询用户个人账户的实际用量或官方配额余额。
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用火山引擎 Podcast AI 模型生成中文双人对话播客。当用户要把文章、报告、话题文本转成播客音频、生成对话式音频内容时使用，需要环境具备火山引擎 APP_ID 和 ACCESS_KEY。支持 mp3/ogg_opus/pcm/aac、语速调节、自定义音色、断点续传。不用于：单人朗读式 TTS（用普通语音合成）、英文播客（模型主要优化中文）、播客文稿本身的撰写（先用写作类 skill 产出文本再来）。
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Generate structured meeting notes from transcripts or pasted text. Use when someone says "meeting notes", "process this transcript", "summarize this call", "notes from today's meeting", or pastes/references a meeting transcript. Auto-enriches attendees from stakeholder directory, routes output to correct project subfolder, flags downstream artifact impacts (PRD, deck, architecture), and proposes CLAUDE.local.md updates. Produces date-first Markdown files.
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Use when the user sends or generates an image, screenshot, video, audio or file and wants it saved, or asks to find past media (that diagram, the mockup from last week). Ingests and searches with local ChromaDB embeddings.
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Run nf-core bioinformatics pipelines (rnaseq, sarek, atacseq) on sequencing data. Use when analyzing RNA-seq, WGS/WES, or ATAC-seq data—either local FASTQs or public datasets from GEO/SRA. Triggers on nf-core, Nextflow, FASTQ analysis, variant calling, gene expression, differential expression, GEO reanalysis, GSE/GSM/SRR accessions, or samplesheet creation.
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Design taste evaluation framework — DVF primary filter, Apple/Google/Jobs design principles as explicit scoring criteria, weighted decision matrix, and option ranking for the DIVERGE wave
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Fast-forwards through remaining waves end-to-end without stopping for review between waves.
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Use when a PERSONAL brand needs AI avatar video at scale — three tool tiers, four workflows for single avatar, translation, batch, and hybrid, reference image intake, face, style, logo, and palette replacement, voice clone pairing, anti-detection, and a QA score, with disclosure-law variants for US FTC, EU AI Act, SEA, and LATAM, covering HeyGen and Synthesia. Trigger on 'AI avatar', 'HeyGen video', 'Synthesia', 'talking head AI video', 'translate my videos with AI', 'I cannot be on camera every day'. Not for — the words the avatar says, see `04-script-video-global`; audio-only voice clone and podcast, see `25-voice-clone-podcast-global`; a company product video edit, see `44-video-editor-brief-global`.
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Use when the user wants a marketing image GENERATED or an image prompt written — personal brand portraits, logos and monograms, campaign key visuals, day-to-day social graphics, editorial art, infographics, web hero mockups, and quote graphics. Reads brand identity from project context, composes an on-brand prompt, generates via gpt-image-2 when an API key exists, otherwise outputs paste-ready prompts for DALL-E 3, MidJourney, Leonardo, or Ideogram. Trigger on 'design an image', 'make me a logo', 'MidJourney prompt', 'key visual for the campaign', 'generate a graphic for this post', 'I need a visual and have no designer'. Not for — briefing a human designer on a static, see `42-image-brief-global`; a slide sequence, see `43-carousel-brief-global`; the brand rules themselves, see `46-brand-guideline-global`; scoring a finished design, see `47-design-review-global`.
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Use when a LIVE campaign needs a new asset the same day — creative fatigue, CTR decay, climbing frequency, or a new angle to test: stripped-down input, duplicate the winning creative and change only what is listed, a one-page template, and a hard SLA so the designer ships in 2-4 hours. Trigger on 'quick brief', 'need an asset fast', 'creative fatigue', 'swap the creative today', 'frequency is too high', 'the ad is dying and we need a new one'. Not for — planning assets before launch, see `41-campaign-asset-list-global`; a full concept brief with time to spare, see `42-image-brief-global`; resizing an existing master, see `50-asset-resize-global`; raising budget on a winner, see `55-scaling-ads-global`.
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Use when the next period content plan must be built from last period DATA — keep and replicate winners, cut losers, at most two new hypotheses, a 70/20/10 mix, a four-week overview, and an owner per slot. Trigger on 'next content plan', 'content plan for next month', 'plan from the audit', 'what should we post next quarter', 'scale what worked', 'plan content using the numbers'. Also use when a report just landed and the user asks what to do with it. Not for — auditing the past period first, see `39-content-audit-global`; turning the plan into a dated calendar, see `01-content-calendar-global`; the next period ADS plan, see `57-next-ads-plan-global`.
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Zero-config goal-to-tasks engine. Takes any goal (software, pentest, business, learning), runs adaptive discovery, generates a validated spec, parses into TaskMaster tasks, creates an implementation plan, and executes with built-in CDD verification. Use when user says "PRD", "product requirements", "I want to build", or any goal-driven phrase.
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Phase 1 of the prd-taskmaster pipeline: brainstorm-driven discovery. Delegates to superpowers:brainstorming in Interactive Mode (one adaptive question at a time), or self-brainstorms in Autonomous Mode when no user is present. Intercepts before the brainstorming chain hands off to writing-plans — this skill owns the exit. Extracts constraints, calibrates scale (Solo / Team / Enterprise), and advances the pipeline to GENERATE.
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Application Security Posture Management persona. Correlates findings from SAST, DAST, and SCA tools, deduplicates noise, maps vulnerabilities to specific code commits, and generates targeted remediation PRs.
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