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Designs and analyzes pooled prime-editor (PE) screens for installing precise genetic variants without bystander confounding. Covers pegRNA design with PRIDICT and PRIDICT2 for predicting per-pegRNA editing efficiency, pegRNA architecture (spacer + scaffold + PBS + RTT), PE2/PE3/PE3b/PEmax variants, MOSAIC in situ saturation mutagenesis, the PRIME pooled-screen methodology (Ren 2023; ~3,699 ClinVar variant screens), chromatin context as a major locus-level determinant of PE efficiency, scaffold-incorporation and indel byproduct quantification with CRISPResso2, and the cross-modal validation strategy of PE + base-editor screens for variant function. Use when designing a pegRNA library for variant installation, choosing between BE and PE for a specific edit, predicting pegRNA efficiency before library synthesis, analyzing PE screen output, distinguishing intended-edit from scaffold-incorporation, or scaling PE screens to thousands of variants.

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Assesses genetic health of populations for conservation with Ne estimation across time horizons (LDNe NeEstimator V2 option-file API + SNeP physical-linkage correction; recent trajectory via GONE/GONE2; deep history via Stairway Plot 2 / dadi / fastsimcoal2 / PSMC), F-statistics, runs of homozygosity binned by length class to date inbreeding, genetic-load decomposition (Bertorelle 2022 realized vs masked), the modern 100/1000 Ne rule (Frankham 2014), Ne/Nc 2-6 orders of magnitude in marine fish (Hauser & Carvalho 2008), tree-sequence forward simulations (SLiM 4 + pyslim + tskit), and the Sukumaran-Knowles caveat against MSC methods for management-unit definition. Use when estimating Ne by time horizon, detecting inbreeding via F_ROH, decomposing genetic load, justifying conservation thresholds, distinguishing ESU/MU/DPS, configuring NeEstimator V2, or correcting LDNe physical linkage.

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Corrects the gene-independent copy-number artifact in CRISPR-Cas9 screens (Aguirre 2016 / Munoz 2016 Cancer Discov) where amplified loci appear essential from DNA-damage burden of simultaneous cuts. Covers the gene-independent DNA-damage / G2-arrest mechanism, CRISPRcleanR (Iorio 2018) unsupervised pre-hoc correction, CERES (Meyers 2017) joint CN + gene-effect model, Chronos (Dempster 2021) DepMap-standard population-dynamics + CN model with lowest residual bias, the decision tree by data availability, the Spearman LFC-vs-CN diagnostic, focal-amplification examples (ERBB2 in HER2+, MYC in colorectal, FGFR1 in head and neck), and CRISPRi/a alternatives that bypass the artifact. Use when screening cancer cell lines, diagnosing essentiality at amplified loci, choosing CRISPRcleanR / CERES / Chronos, deciding whether CN correction is needed before MAGeCK / BAGEL2 / drugZ, or switching from Cas9 to CRISPRi.

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Verify that insulin pump supplies (provided by patient or family) will be available throughout the hospitalization to support continued pump use. Triggered by questions such as "Do we have sufficient pump supplies for the hospital stay?"

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This skill determines whether an adolescent or adult patient meets DSM-5 criteria for gender dysphoria (gender incongruence) by evaluating the presence of at least two of six specified criteria over a minimum 6-month duration, accompanied by clinically significant distress or impairment. It is triggered when a clinician queries whether the patient's reported symptoms align with gender dysphoria, such as persistent incongruence between experienced/expressed gender and designated gender.

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This skill recommends bilateral adrenalectomy for occult or metastatic ectopic ACTH secretion or as a life-preserving emergency treatment in patients with very severe ACTH‑dependent Cushing’s syndrome who cannot be promptly controlled by medical therapy. It is triggered by life‑threatening complications of uncontrolled hypercortisolism such as infection, pulmonary thromboembolism, cardiovascular catastrophe, or acute psychosis.

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Identifies young patients with classic primary aldosteronism features who may proceed directly to unilateral adrenalectomy without adrenal venous sampling based on high probability of unilateral disease. Triggered when a clinician sees a patient <35 years with hypokalemia, unilateral adrenal tumor on CT, and high plasma aldosterone concentration and asks whether AVS can be bypassed.

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Recommends fludrocortisone 100 µg daily for children with primary adrenal insufficiency and confirmed aldosterone deficiency; for infants under 12 months, adds sodium chloride supplementation. Triggered by PAI with aldosterone deficiency, evidenced by salt craving, hyponatremia, hyperkalemia, or elevated renin with low aldosterone.

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Calculates the lowest effective glucocorticoid dose (dexamethasone or prednisone) to normalize blood pressure and serum potassium in glucocorticoid-remediable aldosteronism (GRA/FH-I) by titrating to biochemical and clinical targets. Indicated when initiating medical treatment for confirmed GRA, triggered by findings such as early-onset hypertension, family history of stroke before age 40, spontaneous hypokalemia, or suppressed plasma renin activity with elevated aldosterone.

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Set absolute LDL-C, non-HDL-C, and ApoB treatment goals, PLUS percent LDL-C reduction, for a primary-prevention patient on lipid-lowering therapy per the re-introduced treatment goals in the 2026 ACC/AHA multisociety dyslipidaemia guideline. Covers goals by PREVENT-ASCVD category (low / borderline / intermediate / high), by CAC score, in severe hypercholesterolaemia / FH, in diabetes with multiple ASCVD risk factors, and the ApoB targets once LDL-C and non-HDL-C goals are met. Use when a clinician asks what LDL goal to aim for, LDL target for intermediate risk, non-HDL goal, ApoB target, dual goals on statin, patient at goal or not, how low should LDL go, statin not enough what next. Grounded in the 2026 ACC/AHA multisociety dyslipidaemia guideline.

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When the user wants to create step-by-step technical tutorials, quickstarts, or code walkthroughs. Trigger phrases include "tutorial," "quickstart," "getting started guide," "walkthrough," "step by step," "how to guide," "hands-on guide," or "code tutorial."

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Write the production code that makes a pre-decided set of failing tests pass, within an explicit write_set, following an explicit behavior contract. Used by `/tdd` Step 3 and by `/swarm-dispatch` workers. The caller has already decided architecture, naming, file layout, and abstraction boundaries — this skill executes that recipe with code-structure discipline and a 5-iteration RALPH loop. No scope expansion, no design decisions, no test edits.

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Autonomous experiment loop that optimizes any file by a measurable metric. Inspired by Karpathy's autoresearch. The agent edits a target file, runs a fixed evaluation, keeps improvements (git commit), discards failures (git reset), and loops indefinitely. Use when: user wants to optimize code speed, reduce bundle/image size, improve test pass rate, optimize prompts, improve content quality (headlines, copy, CTR), or run any measurable improvement loop. Requires: a target file, an evaluation command that outputs a metric, and a git repo.

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Automate recruiting and hiring workflows in Ashby -- manage candidates, jobs, applications, interviews, and notes through natural language commands.

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Automate Abyssale tasks via Rube MCP (Composio). Always search tools first for current schemas.

日本語の概要は準備中です。原文の説明を表示しています。

Practical UI refactoring and visual design review inspired by Refactoring UI by Steve Schoger and Adam Wathan. Use when an AI coding agent needs to improve an existing interface, audit visual polish, redesign a rough UI, review frontend code for hierarchy/spacing/typography/color/depth/image issues, or turn a plain implementation into a cleaner product-quality screen. Do not use for creating UI from scratch, scaffolding a new app, building a new landing page, or designing a new design system unless the user explicitly asks to review or refactor an existing interface.

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Search and preview Irasutoya illustrations with the installed `irasutoya` command-line tool. Use this skill whenever the user wants to find, recommend, preview, randomly pull up, or get the page/image URL of an Irasutoya (いらすとや) image — from a topic, scene, Korean/Japanese/English phrase, visual description, emotion, character, object, or action — even if they don't say "Irasutoya" by name but clearly want a free, cute illustration for a slide, 발표자료, blog, doc, or chat. Also use it to optimize vague requests into fast Japanese keyword candidates and to return page/image URLs. Do NOT use it to generate brand-new AI art (e.g. DALL·E, Midjourney), draw charts or diagrams, design logos or icons, cut out/edit existing images, or recommend other stock-image sites — it only searches Irasutoya's existing catalog.

Read-only SRE checkup of any GCP project: deterministic probes of the edge, Cloud Run services, 7-day error logs, Cloud Scheduler, alert policies and uptime checks, Secret Manager and IAM, the data stores and the machine's own scheduled jobs, audited into one fixed status table (LIVE / WARNING / RED / INCONCLUSIVE) with evidence, findings by severity, what could not be checked, and a single OVERALL line delivered as one notification. Parametrised by a per-project manifest, so the same routine runs on every project. Use when the operator says "cloud checkup", "SRE check", "is everything live", "what's healthy / warning / red", "any errors this week", "audit the infra", "weekly checkup", "set up the weekly checkup", before a deploy or demo, or after an incident. Cloud Run first; App Engine and GKE differ only in the serving probes.

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Implement and finalize one ready rolling-wave slice in one invocation while preserving the lifecycle boundary between implementation and independent review. Use when the user wants a slice delivered end to end without manually invoking implement-slice and finalize-slice, wants main-local implementation by default with workers only when delegation pays, followed by mandatory fresh-context finalization, or says deliver, implement and finalize, or take the slice through review.

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An autonomous social network built by agents, for agents.

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Sends voice messages (audio) to Feishu chats using Duby TTS.

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Check EV charger availability (favorites, nearby search) via Google Places.

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Get detailed routing between two points with distance, duration, and optional turn-by-turn directions.

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Meta-skill for pre-accounting automation by orchestrating gmail, deepread-ocr, stripe-api, and xero.

日本語の概要は準備中です。原文の説明を表示しています。