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Install, instrument and verify this running web app from the inside (DOM, network, routing, console and framework state) instead of screenshots or guessing. Drives one real flow end to end and returns a verdict with the file:line to fix. Use when the user asks to set up or install Reticle, when a user-facing change needs proving before you call it done, when a test passes but the UI is broken, or when the user types /reticle.

日本語の概要は準備中です。原文の説明を表示しています。

Verify that a web app change actually works by driving the running app from the inside (DOM, network, routing, console, framework state) instead of screenshots or guessing. Use after any user-facing change, when a fix is claimed but unproven, when a test passes but the UI is broken, or when you need a real verdict rather than "looks right". Also use to install and wire up Reticle in a project that does not have it yet.

日本語の概要は準備中です。原文の説明を表示しています。

Build, deploy, run, monitor, tune, and stop Simulink Real-Time applications. Use when the user wants to build a Simulink Real-Time model, load/start/stop an `.mldatx` on a target, set stop time or start options, drive root inports/playback/parameter stimulation, inspect installed or running apps, tune parameters with `getparam`/`setparam`, read ad hoc signal values with `getsignal`, manage parameter sets, control recording, or choose between file logging, live streaming, and file-log import. Covers `slbuild`, `install`, `load`, `start`, `stop`, `setStopTime`, `tg.Stimulation`, `startRecording`/`stopRecording`, `tg.FileLog.import`, `slrealtime.exportRun`, `getparam`/`setparam`, `getsignal`, and installed app management for Speedgoat targets and Simulink Real-Time Linux devices.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill to run multiple Simulink simulations in parallel with parsim, batchsim, and DesignStudy. TRIGGER when: user asks for parameter sweep, sweeping/varying parameters across simulations, Monte Carlo study, batch simulation, running multiple simulations, parallel simulations, large-scale multiple simulations, any task involving repeated simulations with different parameter values, user mentions having an existing script/function for parameter sweeps, or user wants to migrate from parfor/set_param/for-loop patterns to recommended workflows.

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Phases small variants, SVs, and methylation from Oxford Nanopore and PacBio long reads (read-backed/physical phasing) with WhatsHap, LongPhase, or HiPhase, and haplotags the BAM (HP/PS tags) for allele-resolved downstream analysis. Covers why phase blocks break at het-sparse gaps (read length x heterozygosity), why phasing the VCF is useless until the BAM is haplotagged, the GT-pipe/PS and read HP/PS tag spec, reporting block N50 with switch error, the diploid-assumption/CNV/haploid-region traps, trio phasing as the gold standard, and the boundary to statistical panel phasing. Use when phasing long-read variants, haplotagging reads for allele-specific methylation/expression or phased SVs, choosing WhatsHap vs LongPhase vs HiPhase, trio phasing, or assessing phasing quality.

日本語の概要は準備中です。原文の説明を表示しています。

Turns Hi-C/Micro-C FASTQ into a deduplicated, filtered .pairs file with pairtools and decides whether the library worked. Covers the bwa mem -SP5M / bwa-mem2 / chromap --preset hic alignment idiom (mates mapped as independent single-end reads), pairtools parse vs parse2 and the walks-policy choice (5unique pairwise vs all for Pore-C/Micro-C concatemers), pair-type classification (keep UU and rescued UC), dedup (PCR vs optical/by-tile), select by pair_type/MAPQ/distance, restriction-fragment handling (restrict, Arima dual-enzyme, Micro-C/DNase fragment-free), and allele-specific phasing (pairtools phase to two coolers). The library-QC decision uses % long-range cis as the one-number quality metric, trans as the noise floor, orientation balance as fragment-map-free dangling-end/self-circle QC, and % duplicates as a complexity proxy. Use when processing Hi-C/Micro-C/Omni-C reads into pairs, judging library quality, handling multi-enzyme or restriction-agnostic protocols, or generating allele-specific contacts.

日本語の概要は準備中です。原文の説明を表示しています。

Decides how to preprocess plasma cfDNA sequencing data so the recoverable signal survives - library-prep-aware fragment expectations (dsDNA vs ssDNA/adaptase prep), UMI/duplex consensus with fgbio (ExtractUmisFromBam, GroupReadsByUmi --strategy paired for duplex, CallMolecularConsensusReads vs CallDuplexConsensusReads, FilterConsensusReads min-reads "total s1 s2"), the align->group->consensus->RE-align ordering, and the cfDNA dedup trap where naive coordinate dedup collapses nucleosome-coincident independent molecules. Covers when single-strand consensus suffices vs when duplex is mandatory, the singleton/sensitivity tax at low input, and reading the insert-size histogram as a pre-analytical QC instrument. Use when processing plasma cfDNA reads before fragmentomics, ctDNA mutation calling, or tumor-fraction estimation.

日本語の概要は準備中です。原文の説明を表示しています。

Cross-method decision tree for calling hits in pooled CRISPR screens. Catalogs statistical models (MAGeCK RRA, MAGeCK MLE, BAGEL2, drugZ, JACKS, Chronos, CERES), experimental designs each is built for, failure modes outside design domain, reconciliation when methods disagree, multiple-testing and effect-size thresholds, the order of operations (count -> QC -> CN-correct -> hit-call -> validate), the second-best-sgRNA conservative rule, and consensus-hit strategy. Use when choosing among MAGeCK / BAGEL2 / drugZ / JACKS / Chronos for a given design, reconciling disagreement across two or three methods on the same screen, deciding whether to require consensus, gating downstream validation by hit-confidence tier, or interpreting unstable hit lists across reruns.

日本語の概要は準備中です。原文の説明を表示しています。

Queries PharmGKB / CPIC / DPWG for drug-gene interactions; calls CYP2D6/CYP2C9/CYP2C19/DPYD/TPMT/NUDT15/UGT1A1/SLCO1B1 star alleles and phenotype with PharmCAT, Cyrius (CYP2D6 structural variants), Aldy, Stargazer; applies Caudle 2020 activity-score translation. Use when implementing pharmacogenomic-guided prescribing, applying CPIC vs DPWG guidance, screening HLA risk alleles for ICI / antiepileptics / abacavir, or interpreting compound TPMT+NUDT15 thiopurine risk.

日本語の概要は準備中です。原文の説明を表示しています。

Analyzes base-editing screens for variant function. Covers library design (Hanna 2021 ClinVar-scale CBE screen benchmarked on BRCA1/2, Cuella-Martin 2021 DDR saturation), CBE vs ABE chemistry choice (BE3/BE4 vs ABE7.10/ABE8.20/ABE8e), editing-window math (positions 4-8 from PAM-distal end; 4-7 for ABE7.10), bystander-edit quantification and the variant-call ambiguity it creates, sgRNA-efficiency filtering before hit calling, indel byproduct interpretation, the substitution-vs-indel diagnostic, variant annotation against ClinVar / COSMIC, and the Broad be-validation-pipeline. Use when designing a BE variant screen, choosing CBE vs ABE for a specific edit, interpreting bystander-confounded hits, distinguishing functional signal from indel artifact, integrating CRISPResso2 output with screen scoring, or deciding BE vs PE for SNV installation.

日本語の概要は準備中です。原文の説明を表示しています。

Calls ChIP-seq peaks with MACS3, MACS2, HOMER, or SPP across narrow (TF) and broad (histone) modes. Handles input control matching, fragment-size modeling vs --nomodel, effective genome size, ENCODE-style IDR vs naive overlap, hyper-ChIPable artifacts, and aligner-specific shifts. Use when calling peaks from ChIP-seq alignments, choosing between narrow vs broad mode for a histone mark, deciding model vs nomodel for low-depth data, applying ENCODE pseudoreplicate IDR, or reconciling MACS vs HOMER vs SPP results.

日本語の概要は準備中です。原文の説明を表示しています。

Call accessible chromatin regions from ATAC-seq BAM files using MACS3, MACS2, Genrich, or HMMRATAC. Use when identifying open chromatin from aligned ATAC-seq, choosing between point-source vs HMM peak callers, applying ENCODE-style pseudoreplicate IDR, removing blacklist regions, or fixing 501bp consensus peaks for downstream differential analysis.

日本語の概要は準備中です。原文の説明を表示しています。

Generate pileup data for variant calling using samtools mpileup and pysam. Use when preparing data for variant calling, analyzing per-position read data, or calculating allele frequencies.

日本語の概要は準備中です。原文の説明を表示しています。

Analyzes alternative splicing at single-cell resolution. The first decision is library chemistry — 10X 3' is fundamentally limited (RT primes from poly-A, R2 falls in 3' UTR, <0.1 junction read per cell per AS event). Plate-based full-length methods (Smart-seq3, FLASH-seq, VASA-seq, STORM-seq) and single-cell long-read (MAS-Iso-seq, scISOr-Seq2) are the chemistries that give per-cell isoform structure. Tools include MARVEL (R, Smart-seq integrated), BRIE2 (Bayesian PSI with regulatory features and ELBO_gain test), scQuint (junction-cluster, plate-based; not for 10X), SpliZ (annotation-free Z-score), Psix (graph-smoothness regulated AS), and Sierra (alternative polyadenylation, often confused with AS). Use when analyzing isoform usage in scRNA-seq, identifying cell-type-specific splicing, or determining whether scRNA-seq chemistry supports splicing analysis at all.

日本語の概要は準備中です。原文の説明を表示しています。

Deploys and operates a LiveKit agent in production: shipping a version to LiveKit Cloud and rolling it back, secrets and configuration, the worker process model and prewarming, safe async inside worker processes, provider timeouts and degradation, graceful shutdown, SDK upgrades, and observability. Use when the user says "deploy my agent", "roll back the deployment", "tail the agent logs", "the first call after a restart is slow", "attached to a different loop / event loop is closed", "prewarm the VAD", "shut down without dropping calls", "upgrade livekit-agents safely", "correlate logs by session", or is changing an agent codebase that is already live. Not for designing the agent (building-livekit-agents) or reproducing one bad conversation locally (debugging-livekit-agents).

日本語の概要は準備中です。原文の説明を表示しています。

Use when installing skills from a shared ai-agent-skills library repo. Inspect with `--list` first, prefer `--collection`, and preview with `--dry-run` before installing.

日本語の概要は準備中です。原文の説明を表示しています。

Analyze, standardize, validate, and sync locally maintained skills into agent skill directories with a `skills` CLI-aligned workflow. Use this skill when Codex needs to turn ad-hoc prompt or rules folders into reusable `SKILL.md`-based skills, install or sync one or more local skills from `./skills` into Claude, Cursor, CodeBuddy, Codex, or similar agent directories, or manage local skill path mappings and symlink or copy installation behavior.

日本語の概要は準備中です。原文の説明を表示しています。

Review a business plan from the perspective of the solo founder who has to actually execute it. Asks the brutal operational questions: Can I build this with my time budget? Can I keep it running? Will my cashflow survive? Outputs a verdict — SHIPPABLE NOW / SHIPPABLE WITH DESCOPE / NEEDS HIRE / WRONG STACK. Use when a plan looks great on paper and you want to know whether a solo operator can actually execute it. Triggered by: 'operator review', 'can I actually do this solo', 'execution reality check', '操盘视角', '我能不能干得动'.

日本語の概要は準備中です。原文の説明を表示しています。

24/7 autonomous business operations orchestrator with business health scoring, canary monitoring, and safety guardrails. Schedules and runs all business functions automatically — content publishing, social media posting, outreach sequences, ad monitoring, financial reporting, health checks, and post-deploy verification. Use when the user wants to automate operations, schedule tasks, set up autonomous workflows, or says 'automate this', '24/7', 'run automatically', 'schedule', 'cron', 'autonomous', or 'set and forget'.

日本語の概要は準備中です。原文の説明を表示しています。

Comprehensive skill for Locomotive Scroll smooth scrolling library with parallax effects, viewport detection, and scroll-driven animations. Use this skill when implementing smooth scrolling experiences, creating parallax effects, building scroll-triggered animations, or developing immersive scrolling websites. Triggers on tasks involving Locomotive Scroll, smooth scrolling, parallax, scroll detection, scroll events, sticky elements, horizontal scrolling, or GSAP ScrollTrigger integration. Integrates with GSAP for advanced scroll-driven animations.

日本語の概要は準備中です。原文の説明を表示しています。

Query Fabric lakehouse and warehouse data using DuckDB, either locally or inside a Fabric notebook. Automatically invoke when the user mentions "DuckDB", "query Delta tables locally", or asks to "attach DuckDB to a lakehouse", "query OneLake data", "explore lakehouse data", "data freshness check", "validate data quality", "use DuckDB in Fabric".

日本語の概要は準備中です。原文の説明を表示しています。

When the user wants to configure, audit, or optimize robots.txt. Also use when the user mentions "robots.txt," "crawler rules," "block crawlers," "AI crawlers," "GPTBot," "allow/disallow," "disallow path," "crawl directives," "user-agent," "block Googlebot," "fix robots.txt," "robots.txt blocking," or "search engine crawling." For indexing, use indexing.

日本語の概要は準備中です。原文の説明を表示しています。

Deprecated compatibility entry for Call to Action construction. This component is now maintained in Bricks; install and use component-builder to build a new Call to Action.

日本語の概要は準備中です。原文の説明を表示しています。

Use when making the art for a side-scroller or 2D platformer: side-view pixel-art sprite sheets for a hero and ground enemies (run, walk, idle, attack, hurt cycles, air spin, jump and fall poses) and per-level layers (parallax background and midground, seamless wall texture, platform strip, props). Also use when a magenta key eats part of a sprite, a walk loop will not close, a slash trail keys into a solid block, generated textures do not tile, or poses on one sheet will not split.

日本語の概要は準備中です。原文の説明を表示しています。