1.5万 件 ・ 関連度順
概要と使いどころ
Perform multiple sequence alignment using MAFFT, MUSCLE5, ClustalOmega, or T-Coffee. Guides tool and algorithm selection based on dataset size, sequence divergence, and downstream application. Use when aligning three or more homologous sequences for phylogenetics, conservation analysis, or evolutionary studies.
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Treats a ctDNA assay as a molecule-counting experiment at the Poisson edge and builds its analytical-validation case the measurement-science way. Covers the genome-equivalent currency (~330 haploid copies/ng), the lambda = input_GE x VAF sampling ceiling (lambda>=3 for ~95% detection), the error-suppression ladder (raw NGS ~1e-3 -> single-strand UMI ~1e-4/1e-5 -> duplex <1e-7), the CLSI EP17 LoB/LoD/LoD95/LoQ framework, the per-locus-vs-panel-integrated LoD distinction that lets bespoke MRD reach ppm, contrived/SEQC2 reference standards, and honest LoD reporting conditioned on input mass + consensus depth + replicate detection rate. Use when stating or trusting a sensitivity claim, designing a dilution-series validation, deciding how many genome equivalents are needed at a target VAF, choosing a single-locus vs panel-integrated LoD, or auditing a "detects 0.1% VAF" claim.
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ATAC-seq library quality control -- TSS enrichment, FRiP, fragment-size periodicity, library complexity (NRF/PBC1/PBC2), mitochondrial fraction, and ENCODE 4 thresholds. Use when assessing whether an ATAC-seq library passes ENCODE acceptance criteria, diagnosing transposition artefacts, comparing Omni-ATAC vs standard prep quality, or selecting which replicates to drop before peak calling.
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Perform pairwise sequence alignment using Biopython Bio.Align.PairwiseAligner. Use when comparing two sequences, finding optimal alignments, scoring similarity, and identifying local or global matches between DNA, RNA, or protein sequences.
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Sequence-based deep learning for ATAC-seq using chromBPNet, BPNet, scBasset, or Enformer. Use when correcting Tn5 bias with neural networks beyond k-mer models, predicting per-base accessibility profiles, scoring in silico variant effects at GWAS or rare-variant SNPs, discovering motifs via DeepLIFT/TF-MoDISco from a trained model, or generating cell-type-specific accessibility predictions for unobserved cell states.
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Parse and analyze multiple sequence alignments using Biopython. Extract sequences, identify conserved regions, analyze gaps, work with annotations, and manipulate alignment data for downstream analysis. Use when parsing or manipulating multiple sequence alignments.
日本語の概要は準備中です。原文の説明を表示しています。
Process and analyze single-cell ATAC-seq data with Signac, ArchR, SnapATAC2, or Cell Ranger ATAC. Use when handling 10X scATAC or 10X Multiome (paired RNA+ATAC) data, performing per-cell QC, choosing between ArchR/Signac/SnapATAC2 ecosystems, building per-cluster consensus peaksets, integrating with paired scRNA-seq, doublet detection (AMULET vs ArchR vs scDblFinder), or running pseudobulk differential accessibility per cluster.
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Call accessible chromatin regions from ATAC-seq BAM files using MACS3, MACS2, Genrich, or HMMRATAC. Use when identifying open chromatin from aligned ATAC-seq, choosing between point-source vs HMM peak callers, applying ENCODE-style pseudoreplicate IDR, removing blacklist regions, or fixing 501bp consensus peaks for downstream differential analysis.
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Generate publish-grade SVG+PNG technical diagrams — architecture, data flow, flowchart, sequence, agent-memory, class, ER, state-machine, comparison, timeline, and more — across 8 visual styles. Writes output to tasks/visuals/ or an active plan's visuals/ directory. Requires rsvg-convert (librsvg) for PNG export; degrades gracefully to SVG-only when absent.
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Deep spec analysis on a Confluence page. Reads page (+ children) via the confluence-as wrapper, produces a structured Spec Research Report with requirements, acceptance criteria, gaps, ambiguities, and suggested user stories for human review. Read-only — does NOT create Jira tickets. Triggers: 'analyze spec for PAGE-ID', 'extract requirements from confluence page', 'spec research on URL'. NOT for raw page CRUD (mk:confluence-page); NOT for ticket complexity scoring (mk:planning-engine).
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This skill guides clinicians to reassess glucose monitoring frequency when discontinuing continuous subcutaneous insulin infusion (CSII) or transitioning to multiple daily injections (MDI). It is triggered by phrases such as 'Plan for glucose monitoring after pump stop' or 'Determine SMBG/CGM frequency post-pump' to ensure individualized glycemic goals are met.
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Oracle-bound spec-review check that every structured Rollout prerequisite binds to an enforcement-type acceptance criterion. A prerequisite whose `enforced-by` is missing, dangling, or points at a non-enforcement AC is a BLOCKER (hard-blocks implementation entry via the checker fan-out verdict); a prerequisite left in free prose is ADVISORY. Read-only. Runs in the spec-review fan-out before implementation.
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Expert-level fluid physics knowledge. Use when working with fluid statics, fluid dynamics, Bernoulli equation, viscosity, turbulence, Navier-Stokes equations, boundary layers, vorticity, compressible flow, or surface tension. Also use when the user mentions 'Bernoulli', 'Navier-Stokes', 'Reynolds number', 'turbulence', 'viscosity', 'boundary layer', 'vorticity', 'continuity equation', 'laminar flow', 'pressure', 'buoyancy', or 'surface tension'.
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Expert-level physical chemistry knowledge. Use when working with thermodynamics, chemical kinetics, quantum chemistry, statistical mechanics, spectroscopy, electrochemistry, or surface chemistry. Also use when the user mentions 'Gibbs energy', 'equilibrium constant', 'reaction kinetics', 'rate law', 'activation energy', 'Arrhenius', 'partition function', 'Schrodinger equation', 'molecular orbital', 'electrochemistry', 'Nernst equation', or 'surface adsorption'.
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When writing validation — Form Requests, rules, custom rule objects, request-boundary design — even when the user just says 'validate this input' or 'check the request' without naming it.
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Watches an open pull request's CI until green and fixes failing checks. TRIGGER when: the user wants to watch a pull request's CI, babysit a PR until it goes green, or fix failing CI checks on an open PR. DO NOT TRIGGER when: opening the PR in the first place → pr; rebasing the branch onto its base → rebase.
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Opens a pull request, publishing the current branch as a PR. TRIGGER when: the user wants to open a pull request, publish the current branch as a PR, or take the current work through to an open PR. DO NOT TRIGGER when: only committing changes → commit; babysitting CI after the PR is already open → monitoring-pull-requests.
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Annotated Documents: optional experimental application for standalone HTML with evidence. Use when the user explicitly requests Annotated Documents or a kpopper HTML document with evidence, refreshes an existing kpopper document, or has a standing preference for this application. Generic HTML requests do not select it. Install the HTML runtime before use. Not for the record's own page or an ordinary website. Requests come in any language.
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Especialista em CrewAI - o principal framework multi-agente baseado em papéis utilizado por 60% das empresas da Fortune 500. Abrange design de agentes com papéis e objetivos, definição de tarefas, orquestração de crew, tipos de processo (sequencial, hierárquico, paralelo), sistemas de memória e flows para workflows complexos. Essencial para construir equipes colaborativas de agentes IA. Use quando: crewai, equipe multi-agente, papéis de agentes, crew de agentes, agentes baseados em papéis.
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gcloudやbqコマンド実行時に認証エラー（Reauthentication required等）を検出した場合に使用。エージェントが自動で認証コマンドを実行することを防ぎ、ユーザーに認証を依頼します。
Virtual QA Lead that turns requirements into review-ready, TestRail-importable test cases in one conversation. Use it whenever the user has requirements, a spec, a user story, or acceptance criteria (PDF, DOCX, XLSX, TXT, MD, or pasted text) and wants test cases, a test plan, a checklist, test coverage, a regression suite, or a TestRail/Qase/Zephyr export, even without saying "test cases" ("write tests for this spec", "what should we check here?"). Especially useful when they attach their team's existing test cases to match. When the spec describes an API (endpoints, status codes, schemas, auth, OpenAPI/Swagger), Casely also builds a runnable Postman collection, so use it for "API tests", "Postman collection", or "tests I can run in Newman/CI". Triggers on the same request in any language.
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Record browser or Web UI interaction demos as optimized GIFs using the available built-in browser, state-based frame capture, and deterministic encoding, then publish to a dedicated assets branch when the task includes attaching the GIF to a pull request. Use when asked to make, record, or generate a GIF that demonstrates a browser workflow, and for every pull request that changes product-user-visible GUI behavior, which MUST include a GIF recorded from the pull request's real server and model flow.
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Use when landing a stack of dependent GitHub PRs (A ← B ← C, where each bases on the one below) onto master, merging a PR whose base is another open PR's branch, or whenever a request mentions "stacked PRs", "PR stack", "dependent PRs", or merging several related PRs in sequence. Requires every same-repository dependency chain to use GitHub's official stacked-PR feature before landing so GitHub owns stack-wide rules, CI, ordering, retargeting, and merge state.
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Create, update, and manage GitHub issues using MCP tools. Use this skill when users want to create bug reports, feature requests, or task issues, update existing issues, add labels/assignees/milestones, manage repository labels, set issue fields (dates, priority, custom fields), set issue types, manage issue workflows, link issues, add dependencies, or track blocked-by/blocking relationships. Triggers on requests like "create an issue", "file a bug", "request a feature", "update issue X", "set the priority", "set the start date", "create a label", "rename a label", "list repo labels", "link issues", "add dependency", "blocked by", "blocking", or any GitHub issue management task.
日本語の概要は準備中です。原文の説明を表示しています。