「deletion」の検索結果

Maintain entity references, ownership links, target relationships, deletion cleanup, and network-safe relation state.

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Designs pegRNAs and nicking guides for prime editing (PE) -- choosing the nick/strand, tuning the primer-binding site (PBS) and reverse-transcription template (RTT) as a per-locus panel, selecting the PE system (PE2/PE3/PE3b/PE4/PE5/PEmax/PE7), adding MMR-evading and PAM-disrupting silent edits, appending epegRNA 3' motifs (tevopreQ1/mpknot), and ranking with PRIDICT/DeepPrime. Covers twinPE/PASTE for large insertions and the prime-vs-base-editing decision. Use when designing a scarless point mutation, small insertion/deletion, or any of the 12 base conversions without a double-strand break, when efficiency is low and MMR inhibition or pegRNA stabilization is needed, or when routing a large insertion to an integrase method. Generic guide scoring and base editing are separate skills.

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Quantifies CRISPR editing outcomes with CRISPResso2 (Clement 2019 Nat Biotechnol) across Cas9-nuclease (indels, HDR), CBE and ABE base editors (target conversion + bystander), and prime editor (pegRNA-templated) modes. Covers single-amplicon (CRISPResso), multi-sample batch (CRISPRessoBatch), pooled-amplicon (CRISPRessoPooled), WGS off-target (CRISPRessoWGS), and sample-comparison (CRISPRessoCompare) workflows; quantification-window math that controls what is called edited; substitution-vs-indel diagnostic to distinguish BE from Cas9 contamination; MMEJ deletion pattern interpretation; allele-frequency tables; and failure modes from amplicon misalignment or contamination. Use when quantifying editing from amplicon sequencing, choosing CRISPResso mode by design, distinguishing intended edits from bystanders and indel byproducts, debugging low-alignment runs, or generating publication-grade editing reports.

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Detect single-nucleotide crosslink (CL) sites in CLIP-seq data using truncation patterns (iCLIP/eCLIP CITS), crosslink-induced mutations (HITS-CLIP CIMS deletions, PAR-CLIP T-to-C), or HMM/kernel-density methods (PureCLIP, PARalyzer, CTK). Use when single-nucleotide resolution is required for motif registration (mCross), allele-specific binding (BEAPR), variant-effect prediction, or comparing crosslink chemistry across CLIP variants.

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Apply GDPR-compliant engineering practices across your codebase. Use this skill whenever you are designing APIs, writing data models, building authentication flows, implementing logging, handling user data, writing retention/deletion jobs, designing cloud infrastructure, or reviewing pull requests for privacy compliance. Trigger this skill for any task involving personal data, user accounts, cookies, analytics, emails, audit logs, encryption, pseudonymization, anonymization, data exports, breach response, CI/CD pipelines that process real data, or any question framed as "is this GDPR-compliant?". Inspired by CNIL developer guidance and GDPR Articles 5, 25, 32, 33, 35.

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Set up Claude Code git safety guardrails that block dangerous git commands before execution. Use when the user asks to prevent git push, hard reset, git clean, destructive checkout/restore, or branch deletion from Claude Code hooks.

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Design and enforce privacy and data protection controls across application, API, and database layers. Use when tasks involve PII handling, retention/deletion policies, consent, data minimization, auditability, or compliance-oriented architecture changes.

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Analyze Windows Shellbag registry artifacts to reconstruct folder browsing activity, detect access to removable media and network shares, and establish user interaction with directories even after deletion using SBECmd and ShellBags Explorer.

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Anti-forensics and evidence destruction techniques for red team operators conducting authorized engagements. Covers log clearing on Windows (wevtutil, Clear-EventLog, ETW provider patching) and Linux (journal truncation, utmp/wtmp binary editing, syslog manipulation), timestamp manipulation via Timestomp and SetMACE to defeat timeline analysis, filesystem-level anti-forensics including NTFS Alternate Data Streams for payload hiding and secure deletion with sdelete/shred, memory artifact removal to counter live forensics, disk artifact manipulation targeting MFT entries and USN journal records, network forensics evasion through encrypted C2 channels and DNS-over-HTTPS tunneling, and anti-VM/sandbox detection to avoid dynamic analysis environments. Tools: Timestomp, wevtutil, sdelete, shred, MimiPenguin, Invoke-Phant0m. Aligns to MITRE ATT&CK T1070 (Indicator Removal), T1027 (Obfuscated Files or Information), T1497 (Virtualization/Sandbox Evasion). Each technique includes the forensic artifact it targets, t...

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Architecture and systems design for agents that retain bounded state across sessions. Covers durable event history, owned state, derived memory, retrieval indexes, framework evaluation, recovery, deletion, and rebuild behavior. Uses constructed implementation examples and workload calibration rather than universal vendor, latency, similarity, retention, or cost claims. Activate on: "always-on agent", "persistent agent architecture", "episodic memory system", "agent memory design", "long-running agent", "stateful agent", "agent that remembers", "MemGPT architecture", "Letta deployment", "/always-on-agent-architecture". NOT for: choosing what data to feed the agent (use always-on-agent-inputs), brainstorming applications (use always-on-agent-applications), safety and privacy concerns (use always-on-agent-safety), general agentic patterns (use agentic-patterns).

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