1.3万 件 ・ 関連度順
概要と使いどころ
Read biological sequence files (FASTA, FASTQ, GenBank, EMBL, ABI, SFF) using Biopython Bio.SeqIO. Use when parsing sequence files, iterating multi-sequence files, random access to large files, or high-performance parsing.
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Use when the user asks to "build a B2B cold-outbound sequence", "design reply-triage branching", "plan a domain warmup / sending throttle", or "make my outbound CAN-SPAM / opt-in compliant"; produces a multi-step outbound sequence with reply-triage branches (positive / objection / referral / not-now / opt-out), a warmup + send-throttle ramp schedule, jurisdiction opt-in/CAN-SPAM guardrails (guidance, not legal advice), and a SEND S-dimension read. Not for B2C lifecycle flows — use email-sequence-designer; not for the consent record — use consent-registry; not for computing EQS — use email-quality-auditor. B2B冷启动外联序列/回复分流/域名预热
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Use when the user asks to "design a welcome flow", "set up an abandoned-cart sequence", "build a light re-engagement branch inside a lifecycle flow", or "plan a cold-outbound sequence"; produces general lifecycle automation flows (welcome, cart, browse-abandon, post-purchase, in-flow re-engagement, B2B cold outbound) with trigger, step timing, branch/exit conditions, goal, frequency governance (send caps, quiet hours, fatigue guardrail), a sunset path, and a SEND N-dimension score. Not for the closed-loop win-back / re-consent (re-permission) program on a lapsed cohort — use reactivation-specialist; not for writing each email's copy — use email-creative-builder; not for computing EQS or the N1 unsubscribe veto — use email-quality-auditor. 邮件自动化流程设计/购物车挽回/流失召回序列
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Use when analyzing FASTQC quality reports from sequencing data, identifying quality issues in NGS datasets, or troubleshooting sequencing problems. Interprets quality metrics and provides actionable recommendations for RNA-seq, DNA-seq, and ChIP-seq data.
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AI DevKit · Requirements phase guidance for starting features and reviewing requirements. Use when the user wants to capture a new requirement, clarify product scope, initialize feature docs, review requirements, or run dev-lifecycle phases 1-2.
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Request a reasonable accommodation at work or in education — frame it around the barrier and the adjustment (not your diagnosis), cite the right process, and navigate the back-and-forth constructively. Use when someone says 'I need a workplace accommodation', 'request reasonable adjustments', 'ADA/Equality Act accommodation', or 'how do I ask for accommodations for my disability/condition'. Produces the request letter, a barriers-and-adjustments map, disclosure guidance, and a plan for the interactive process. Not legal advice — routes to the formal process and to advocacy where needed.
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Generates draft requirements from Simulink models. Use when drafting or updating requirement artifacts from a model. Prefers Requirements Toolbox (.slreqx) when available; falls back to structured YAML.
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Discovers, classifies, filters, and quantifies full-length transcript isoforms from PacBio Iso-Seq/Kinnex (HiFi) and Oxford Nanopore (cDNA/direct-RNA) long reads, using the isoseq+pigeon pipeline, SQANTI3, and ONT tools (IsoQuant, FLAIR, Bambu, StringTie2). Covers why a novel isoform is an artifact until proven otherwise (RT template-switching, intra-priming, and 5' degradation manufacture junctions and truncations), the SQANTI3 structural categories and their trust order, the Kinnex skera-split step, orthogonal CAGE/poly-A/short-read-junction validation, and why long-read isoform quantification needs EM. Use when building a full-length isoform catalog, classifying/filtering long-read transcripts, running Iso-Seq or ONT cDNA/dRNA analysis, or judging novel-isoform reliability.
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Nominates and assesses CRISPR off-target sites genome-wide. Enumerates candidate sites by mismatch and bulge tolerance with Cas-OFFinder/CRISPRitz, ranks them with the published CFD score (SpCas9-only, relative ranker) or MIT/CRISTA/energy models, runs variant-aware screening against gnomAD/individual genomes (CRISPRme), and frames the empirical genome-wide discovery assays (GUIDE-seq, CIRCLE-seq, CHANGE-seq, DISCOVER-seq, Digenome-seq) and high-fidelity nuclease choice (HiFi Cas9, Sniper-Cas9, eSpCas9, SpCas9-HF1). Use when assessing guide RNA specificity, choosing among candidate guides, screening a therapeutic guide against population variation, or planning empirical off-target validation. Distinguishes predicted vs detected vs validated. On-target activity scoring and deaminase (Cas-independent) base/prime-editor off-targets are separate skills.
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Assigns pathogen lineages (SARS-CoV-2 Pangolin UShER mode; Nextclade clade + QC; pango-designation alias resolution) and tracks variant frequencies over time using Nextstrain (Augur + Auspice), wastewater deconvolution (Freyja, COJAC, alcov, lineagespot), lineage-fitness modelling (multinomial logistic), and recombinant detection (3SEQ, RDP4, Bolotie). Covers Pangolin pangolin-data and Nextclade dataset version pinning (mandatory; lineage-defining mutations change with dataset), Freyja barcode forward-only date constraint, ARTIC primer scheme churn (V3/V4/V4.1/V5.3.2/Midnight) with dropout regions, and recombinant X-prefix designation lag. Use when assigning Pango lineages and Nextclade clades to viral consensus sequences, building Nextstrain Augur surveillance pipelines, deconvolving wastewater into lineage frequencies with Freyja, tracking lineage frequencies over time, handling ARTIC primer dropouts, or running surveillance for SARS-CoV-2/influenza/Mpox/RSV/H5N1/measles.
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Performs differential expression on bulk RNA-seq count data with DESeq2's negative-binomial GLM, Wald and LRT testing, apeglm/ashr/normal LFC shrinkage, independent filtering, Cook's outlier handling, VST/rlog transforms, and design formulas including paired, batch, and interaction terms. Use when running bulk DE, choosing DESeq2 over edgeR or limma-voom, building a paired or interaction design, applying LFC shrinkage for ranking or GSEA, choosing Wald vs LRT, troubleshooting padj=NA, picking VST vs rlog, importing salmon/kallisto via tximport, or analyzing prokaryotic RNA-seq.
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Design and write email campaigns and sequences including onboarding flows, lifecycle campaigns, transactional emails, newsletters, broadcast sends, and launch and announcement emails. Use this skill whenever the user wants to write email copy, plan an email sequence, design an onboarding drip, or set up lifecycle email campaigns. Triggers on email sequence, drip campaign, onboarding email, lifecycle email, welcome email, transactional email, newsletter, email broadcast, launch email, announcement email, nurture sequence, abandoned cart, re-engagement, win-back. Also triggers when planning email automation flows or writing email subject lines for campaigns.
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Design a scope and sequence showing vertical and horizontal curriculum coherence across a programme or year. Use when building new programmes, restructuring subjects, or ensuring progression.
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When the user wants to create or optimize an email sequence, drip campaign, automated email flow, or lifecycle email program. Also use when the user mentions "email sequence," "drip campaign," "nurture sequence," "onboarding emails," "welcome sequence," "re-engagement emails," "email automation," or "lifecycle emails." For in-app onboarding, see onboarding-cro.
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Run nf-core bioinformatics pipelines (rnaseq, sarek, atacseq) on sequencing data. Use when analyzing RNA-seq, WGS/WES, or ATAC-seq data—either local FASTQs or public datasets from GEO/SRA. Triggers on nf-core, Nextflow, FASTQ analysis, variant calling, gene expression, differential expression, GEO reanalysis, GSE/GSM/SRR accessions, or samplesheet creation.
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Run CodeQL database creation and security queries, add data-extension models, or process CodeQL SARIF. Use when CodeQL is explicitly requested; use security-review for a broader manual security review.
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Mathematical visualization skill for equations, proofs, and geometric concepts. **Triggers when:** - User mentions equations, formulas, or mathematical expressions - Request involves mathematical proofs or derivations - Content includes geometric relationships - User mentions LaTeX, calculus, algebra, geometry, trigonometry - Patterns: "equation", "formula", "prove", "derive", "graph", "plot" **Capabilities:** - LaTeX equation rendering with color-coded components - Function graphing and transformations - Geometric constructions and proofs - 3D mathematical surfaces - Step-by-step derivations with highlights
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Manages deletion requests for children's personal data. Covers parental-initiated versus child-initiated requests, age of capacity assessment, identity verification, scope determination, third-party notification obligations, and regulatory timelines under GDPR Art. 17, COPPA Section 312.6, and UK AADC Standard 15. Keywords: deletion, children, right to erasure, parental request, data deletion, COPPA.
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Automatically derives TLA+ properties (invariants, safety, liveness) from natural-language requirements or structured requirement documents. Resolves ambiguities, asks clarifying questions for underspecified requirements, and outputs TLA+-compatible property definitions with semantic explanations. Use when translating system requirements, specifications, or behavioral constraints into formal TLA+ temporal logic properties for verification with TLC model checker.
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Compares old and new requirement documents, analyzes code repository impact, and generates detailed modification plans. Use when Claude needs to: (1) Compare requirement versions and identify changes, (2) Map requirement changes to code components, (3) Identify components to modify, delete, or add, (4) Analyze dependencies and integration points, (5) Assess test impact, (6) Generate comprehensive modification plans in Markdown format. Supports text/Markdown requirements and analyzes feature-level, functional, and API changes.
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Verify that design documents, code implementations, and tests fully cover all specified requirements. Use this skill when validating requirement traceability, conducting design reviews, assessing implementation completeness, checking test coverage against requirements, performing compliance audits, or identifying missing functionality. Produces coverage reports showing which requirements are satisfied, partially satisfied, or missing.
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Generate concise, structured summaries of requirements for quick team understanding. Use when analyzing requirements from text documents (MD, TXT, DOCX) or technical specifications to create bullet-point summaries that highlight core functionality and dependencies/constraints. Ideal for sprint planning, stakeholder updates, team onboarding, or any situation requiring rapid comprehension of requirement documents.
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Extract and summarize core features, constraints, and priorities from requirement documents. Use when analyzing PRDs, RFCs, business requirements, user stories, epics, or any requirement documentation to identify must-have features, technical constraints, priority levels (P0/P1/P2/MoSCoW), and key decision points. Triggers when users ask to summarize, analyze, extract, or understand requirements from documents.
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Hardware verification tool for checking functional equivalence between two RTL designs (Verilog). Use when users need to: (1) Verify if two RTL versions are functionally equivalent, (2) Compare original vs. refactored RTL code, (3) Validate design changes or optimizations, (4) Identify semantic vs. cosmetic differences, (5) Generate counterexamples for non-equivalent designs. Analyzes interface alignment, state variables, logic differences, and produces detailed equivalence verdicts with plain language explanations. Particularly effective for design verification, code reviews, and regression testing of RTL modifications.
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