「ols」の検索結果

Guide to quality filtering raw VCF files before computing summary stats (Ts/Tv ratio, variant counts, AF distributions). Covers detecting raw VCFs via FILTER column and QUAL inspection, QUAL-based filtering with bcftools, Ts/Tv interpretation, and when NOT to filter. Read before any variant-level QC task. See bcftools-variant-manipulation for advanced filters, gatk-variant-calling for caller config, samtools-bam-processing for upstream alignment QC.

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CLI for VCF/BCF: filter, merge, annotate, query, normalize, compute stats. Core post-variant-calling: quality filtering, multi-sample merging, rsID annotation, genotype extraction. Samtools companion in HTSlib. Use GATK for complex indel realignment during calling; use VCFtools for population genetics stats.

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protocols.io REST API: search and fetch wet-lab, bioinformatics, and clinical protocols by keyword, DOI, or category, with steps, reagents, materials, equipment, timing. Public access free; auth needed for private or publishing. Pair with opentrons-protocol-api or benchling-integration to execute.

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Persistent compounding memory for AI agents. 5 default MCP tools: session_start, session_end, remember, recall, check. Full surface: 6 tools with --full (adds check_action); 13 tools with AR_EXTRAS=1 --full. Two-verb model: inhale (session_start) and exhale (session_end). Correction-first memory with decision trail tracking, watch_for warnings, palace rooms with salience scoring, cross-project insight matching, same-day journal merging, ambient recall hooks. Local markdown only. Zero cloud, zero telemetry, Obsidian-compatible. Optional Supabase backend: when configured via `ar setup supabase`, recall() uses pgvector cosine similarity on OpenAI/Voyage embeddings instead of keyword search — same API, semantic understanding. Gracefully degrades to local search if not configured.

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Provides and generates LangChain4j tool and function calling patterns: annotates methods as tools with @Tool, configures tool executors, registers tools with AiServices, validates tool parameters, and handles tool execution errors. Use when building AI agents that call tools, define function specifications, manage tool responses, or integrate external APIs with LLM-driven applications.

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Applitools integration. Manage data, records, and automate workflows. Use when the user wants to interact with Applitools data.

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BED file format fundamentals, creation, validation, and basic operations. Covers BED3 through BED12 formats, coordinate systems, sorting, and format conversion using bedtools and pybedtools. Use when working with genomic coordinates or preparing interval files for downstream tools.

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First-time setup for protein design tools. Use this skill when: (1) User is new and hasn't run any tools yet, (2) Commands fail with "file not found" or "modal: command not found", (3) Modal authentication errors occur, (4) User asks how to get started or set up the environment, (5) biomodals directory is missing or tools aren't working.

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Skill do analizy i tworzenia umów według polskiego prawa, ze szczególnym uwzględnieniem umów B2B, IP i IT (body leasing, NDA, wdrożenia, SaaS, przeniesienie praw autorskich, ugody). Powstał w Kancelarii Radców Prawnych Żurawska Piotrowski i Wspólnicy (ktzr.pl). Używaj zawsze gdy użytkownik prosi o przeanalizowanie polskiej umowy, audyt ryzyk umownych, wygenerowanie nowej umowy w stylu KTZR, dodanie/edycję klauzuli, sprawdzenie spójności umowy lub gdy wkleja/załącza polski dokument umowny do oceny. Stosuj również gdy pojawia się pojęcie "Złote Reguły KTZR", "essentialia negotii", "baza klauzul KTZR" lub gdy użytkownik wspomina o kancelarii KTZR / swojej kancelarii.

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Build heads-up display (HUD) dashboards for AI coding agents — show context usage, active tools, running sub-agents, and task progress in real-time. Use when: monitoring AI agent activity, building developer tools for AI-assisted coding, creating status dashboards for agent workflows.

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Build CLI tools with Commander.js. Use when creating command-line applications, parsing arguments, implementing subcommands, or building developer tools with flags and options.

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Expert in building custom tools that solve your own problems first. The best products often start as personal tools - scratch your own itch, build for yourself, then discover others have the same itch. Covers rapid prototyping, local-first apps, CLI tools, scripts that grow into products, and the art of dogfooding. Use when "build a tool, personal tool, scratch my itch, solve my problem, CLI tool, local app, automate my, build for myself, " mentioned.

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Build MCP tools that are sticky for vibe coders and powerful for developersUse when "Designing new MCP tools, Improving tool UX or DX, Writing error messages, Planning tool naming, Discussing user onboarding, Making tools "sticky", Vibe coder experience, mcp, product, ux, dx, vibe-coding, onboarding, developer-experience, tool-design" mentioned.

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Model Context Protocol expert for building MCP servers, tools, resources, and client integrationsUse when "mcp server, model context protocol, claude code extension, building ai tools, tool definition, mcp transport, stdio transport, sse transport, resource provider, prompt template, mcp, model-context-protocol, claude-code, ai-tools, llm-integration, anthropic, server, protocol" mentioned.

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Master the AI tools that supercharge software development. From code completion to full application generation, write better code faster and automate the tedious parts. Use when "AI coding, code assistant, Copilot, Cursor, code generation, AI developer tools, development, coding, ai-assistant, productivity, code-generation" mentioned.

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用 AI agent 赚钱 / agent 商业化 (创业 + 副业 + 咨询 + indie hacker) (Monetizing AI agents — building businesses (B2B SaaS / B2C tools / consulting / indie hacker / API arbitrage) around AI agent products and services) Master OS — automated mastery of Monetizing AI agents — building businesses (B2B SaaS / B2C tools / consulting / indie hacker / API arbitrage) around AI agent products and services: top builders' mental models, tool stack, current workflows, jargon, and where to keep up. Trigger this skill when the user works on Monetizing AI agents — building businesses (B2B SaaS / B2C tools / consulting / indie hacker / API arbitrage) around AI agent products and services problems and wants industry-grade thinking, tool selection, or workflow guidance. 触发词：「agent business」「monetize agents」「AI agent SaaS」「agent creator」「agent indie hacker」

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Read this skill before the first step that uses the built-in browser, the browser pane inside the Claude desktop app (also called the in-app browser, the browser pane, Claude's browser, or "your own browser"), whose tools are named mcp__Claude_Browser__* when the session runs in the desktop app and mcp__remote-devices__Claude_Browser__* when a cloud session is linked to the person's computer; before those tools are turned on there may be a single enable__mcp__remote-devices__Claude_Browser tool instead. It covers the pane's persistent sign-ins, tabs and preview_start, reading pages as text, site approvals, what the pane cannot open, and what to do when it cannot be reached. It is not for Claude in Chrome (mcp__claude-in-chrome__* tools), which has its own skill, and it does not decide which browser to use.

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Tests in real browsers via Chrome DevTools MCP. Use when building or debugging anything that runs in a browser. Use when you need to inspect the DOM, capture console errors, analyze network requests, profile performance, or verify visual output with real runtime data. Requires the chrome-devtools MCP server to be configured.

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SEO/GEO Agent Operations SOP v2 — Rebuilt from real-world operations (analook.com 0→launch, GSC incident, DataForSEO monitoring, gingiris.tools post-migration recovery). What's new in v2: • Step-by-step onboarding (Q1→Q6), multiple choice wherever possible — no big forms • Mandatory daily SEO log — first item is always GSC token status check • Data cross-validation rule: any external metric must come from ≥2 sources before use • Backlink progress tracking (6-week wait → check → alert if not indexed) • New vs. established site strategy matrix (new site <6mo: KD≤20; mature: KD up to 35+) Real incidents baked in: GSC token expired silently for 2 weeks (analook.com), P0 keywords dropped out of Top 100 after domain migration (gingiris.tools), traffic data off by 17× from single-source DataForSEO. Triggers: "SEO agent" | "SEO daily report" | "GSC monitoring" | "DataForSEO" | "GEO agent" | "backlink tracking" | "IndexNow" | "competitor comparison page" | "SEO SOP" | "autonomous SEO" | "GEO triple combo" 🇨🇳 SEO/GEO Agent 运营 SOP v2 — 基于真实运营经验重写（analook.com 从零建站 + GSC 断档 2 周 + DataForSEO 监控 + gingiris.tools 迁移权重恢复）。 对话式开局、强制日报（GSC token 是第一项）、数据交叉验证铁律、外链进度追踪、新老站区分策略。

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Query and invoke tools on MCP servers using fastmcp list and fastmcp call. Use when you need to discover what tools a server offers, call tools, or integrate MCP servers into workflows.

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Managing local .NET tools with dotnet-tools.json for consistent tooling across development environments and CI/CD pipelines.

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Central authority for Claude Agent SDK (TypeScript and Python SDKs). Covers SDK installation, authentication (Anthropic key, Bedrock, Vertex), sessions and resumption, forking sessions, streaming vs single mode, custom tools, permissions (allowedTools, disallowedTools, permissionMode), MCP integration, system prompts (CLAUDE.md, appendSystemPrompt, outputStyle), cost tracking, todo tracking, structured outputs, hosting patterns, plugins, and SDK branding guidelines. Assists with building custom agents, configuring SDK options, and troubleshooting SDK issues. Delegates 100% to docs-management skill for official documentation.

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Survey the whole tdmcp project and produce a prioritized list of NEW features it could implement — across artist controls, CLI/DX, AI/LLM integration, and TouchDesigner depth. Use whenever the user wants to brainstorm, discover, survey, list, or audit what features/tools/effects/controls/commands/prompts/capabilities tdmcp *could* add, asks 'what could we build / what's missing / what are the opportunities / ideas for the project', or wants a feature backlog or gap analysis. Also for follow-ups: re-run, refresh, update, re-survey, deepen, or re-prioritize the backlog, or survey just one surface (e.g. 'CLI ideas only'). This is the IDEATION harness — it produces a list to decide from; it does NOT build. When the user has already chosen a feature and wants it implemented/shipped/added, use tdmcp-pipeline instead.

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Add WebMCP tools to a website codebase so browser agents can act through the site's own logic instead of scraping the page. Use when asked to make a site agent-ready, add WebMCP or document.modelContext tools, or implement site tools with @nekuda/webmcp-sdk.

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