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Vue 3 testing best-practices review: Vitest + Vue Test Utils for components, composables, Pinia, Router, async/Suspense, forms, a11y, plus Playwright E2E strategy. Advisory only.

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Vue 3 authoring/review: components, composables, reactivity, Pinia, Pinia Colada, file routing, forms. Composition API + script setup. Auto-activates on .vue files. Deep review: mk:vue-best-practices.

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Angular v20+ patterns: components, signals, services, forms, routing, HTTP, testing, SSR. Auto-activates on .ts files in Angular projects (angular.json detected).

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Use for Vue 3 testing best-practices review and recommendations — designing and auditing Vitest + Vue Test Utils tests for components, composables, Pinia stores, Vue Router, async/Suspense, Teleport, forms, and accessibility, plus Playwright E2E test-design strategy. Advisory/review only — recommends patterns and flags test smells; does not run tests. Complements mk:vue-best-practices (feature code). Invoke for "review my Vue tests", "how should I test this component/composable/store", or "Vue test best practices".

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Use when writing, reviewing, or refactoring Vue 3 code — components, composables, reactivity, Pinia state, Pinia Colada data-fetching, file-based routing, and forms. Targets Composition API + <script setup> + TypeScript. Auto-activates on .vue files. For deep best-practices review/recommendations or the full ordered workflow (built-in components, animations, performance pass), use mk:vue-best-practices.

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Session-persistent browser automation via Playwright CLI — form filling, screenshots, data extraction, multi-step flows. Use when the user needs to navigate websites, interact with web pages, fill forms, take screenshots, test web applications, or extract information across multiple requests. NOT for AI-driven long-autonomous flows (see mk:agent-browser); NOT for generating .spec.ts E2E code (see mk:qa-manual).

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Use when working with Angular code — components, signals, services, forms, routing, HTTP, testing, SSR, or tooling. Targets Angular v20+ modern patterns. Auto-activates on .ts files in Angular projects (detected via angular.json).

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Create beautiful, accessible user interfaces with shadcn/ui components (built on Radix UI + Tailwind), Tailwind CSS utility-first styling, and canvas-based visual designs. Use when building user interfaces, implementing design systems, creating responsive layouts, adding accessible components (dialogs, dropdowns, forms, tables), customizing themes and colors, implementing dark mode, generating visual designs and posters, or establishing consistent styling patterns across applications.

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Generate, fill, and assemble PDF documents at scale. Handles legal forms, contracts, invoices, certificates. Supports form filling (pdf-lib), template rendering (Puppeteer, LaTeX), digital signatures (DocuSign), and document assembly. Use for legal tech, HR automation, invoice generation. Activate on "PDF generation", "form filling", "document automation", "digital signatures". NOT for simple PDF viewing, basic file conversion, or OCR text extraction.

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Performs root cause analysis on DAG execution failures. Traces failure propagation, identifies systemic issues, and generates actionable remediation guidance. Activate on 'failure analysis', 'root cause', 'why did it fail', 'debug failure', 'error investigation'. NOT for execution tracing (use dag-execution-tracer) or performance issues (use dag-performance-profiler).

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Detect crisis signals in user content using NLP, mental health sentiment analysis, and safe intervention protocols. Implements suicide ideation detection, automated escalation, and crisis resource integration. Use for mental health apps, recovery platforms, support communities. Activate on "crisis detection", "suicide prevention", "mental health NLP", "intervention protocol". NOT for general sentiment analysis, medical diagnosis, or replacing professional help.

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Validates DAG structures, performs topological sorting, detects cycles, and resolves dependency conflicts. Uses Kahn's algorithm for optimal execution ordering. Activate on 'resolve dependencies', 'topological sort', 'cycle detection', 'dependency order', 'validate dag'. NOT for building DAGs (use dag-graph-builder) or scheduling execution (use dag-task-scheduler).

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Operations, debugging, and optimization for DAG workflows. Performs root cause analysis on failures, profiles execution performance, aggregates results from parallel branches, bridges context between nodes, and learns patterns from execution history. Activate on "DAG failed", "why did it fail", "root cause", "performance profile", "aggregate results", "merge branches", "execution patterns", "optimize DAG". NOT for planning DAGs (use dag-planner), executing DAGs (use dag-runtime), or validating outputs (use dag-quality).

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Builds, validates, schedules, and dynamically modifies DAG execution graphs. Decomposes problems into nodes with dependencies, performs topological sorting, detects cycles, resolves conflicts, and schedules wave-based parallel execution. Use when designing a DAG structure, validating dependencies, planning execution order, or modifying a DAG at runtime. Activate on "build DAG", "plan workflow", "DAG dependencies", "topological sort", "schedule execution", "modify DAG", "replan". NOT for executing DAGs (use dag-runtime), validating outputs (use dag-quality), or matching skills to nodes (use dag-skills-matcher).

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AI-powered career biographer that conducts empathetic interviews, extracts structured career narratives, and transforms professional stories into portfolios, CVs, and personal brand assets. This skill should be used when users want to document their career journey, create professional portfolios, generate CVs, or craft compelling career narratives.

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Expert in 2000s-era music visualization (Milkdrop, AVS, Geiss) and modern WebGL implementations. Specializes in Butterchurn integration, Web Audio API AnalyserNode FFT data, GLSL shaders for audio-reactive visuals, and psychedelic generative art. Activate on "Milkdrop", "music visualization", "WebGL visualizer", "Butterchurn", "audio reactive", "FFT visualization", "spectrum analyzer". NOT for simple bar charts/waveforms (use basic canvas), video editing, or non-audio visuals.

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Performs alchemical free-energy calculations including relative binding free energy (RBFE / FEP+) and absolute binding free energy (ABFE) via OpenFE, FEP+, GROMACS, AMBER pmemd, and OpenMM with explicit lambda scheduling, soft-core potentials, MBAR/BAR analysis, cycle-closure validation, and protocol-appropriate enhanced sampling. Compares ML alternatives (Boltz-2 affinity, DeepDock). Use when ranking analogs by binding affinity beyond docking accuracy, performing prospective lead optimization, or validating SAR predictions.

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Normalizes and transforms RNA-seq count matrices for DE, visualization, clustering, and ML. Covers between-sample (TMM, TMMwsp, RLE/median-of-ratios, upper quartile), within-sample (TPM, FPKM/RPKM), variance-stabilizing (VST, rlog, log-CPM), GC-content correction (cqn, EDASeq), and single-cell (scran deconvolution, scanpy normalize_total). Encodes the composition-bias rationale, the "most genes not DE" assumption and its catastrophic failure modes (MYC amplification, apoptosis, viral host shutoff, prokaryotic stress), the "lengthScaledTPM is not TPM" naming trap, the "TPM is not for DE" rule, the blind=TRUE vs FALSE decision, ERCC spike-in normalization (SBN), and the single-cell zero-inflation breakdown of TMM/RLE. Use when choosing or applying normalization, debugging shifted-MA-plot diagnostics, handling zero-heavy single-cell data, or correcting GC bias.

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Corrects fluorophore spillover (conventional compensation) or spectral overlap (spectral unmixing) and applies variance-stabilizing transforms (logicle/biexponential, arcsinh, log) for flow and mass cytometry. Covers spillover-matrix estimation from single-stain controls, AutoSpill, the spillover spreading matrix and why panel design (not compensation) bounds resolution, compensate-then-transform ordering, and arcsinh cofactor choice (5 for CyTOF, ~150 for fluorescence, per-channel via flowVS). Use when correcting spectral overlap, preparing data for gating/clustering, choosing logicle vs arcsinh, deciding a cofactor, or distinguishing compensation from spectral unmixing.

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Performs differential expression on bulk RNA-seq count data with edgeR's negative-binomial GLM and quasi-likelihood F-test framework. Covers DGEList construction, filterByExpr, TMM/TMMwsp normalization, robust dispersion estimation, glmQLFit/glmQLFTest, TREAT for magnitude-bounded hypotheses, contrasts via no-intercept designs, voom and voomWithQualityWeights for heterogeneous samples, and the edgeR v4 bias-corrected APL changes. Use when running bulk DE with edgeR, choosing edgeR over DESeq2 (small n, transcript DE via catchSalmon, large samples), needing TREAT for a fold-change-threshold hypothesis, troubleshooting v3-to-v4 reproducibility, building paired or interaction designs, or handling library-quality heterogeneity.

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Runs JACKS (Joint Analysis of CRISPR/Cas9 Knockout Screens; Allen et al 2019 Genome Research) which models per-sgRNA log-fold-change as the product of a treatment-dependent gene-essentiality term and a treatment-independent guide-efficacy term. Covers the Bayesian decomposition math, the hierarchical efficacy prior shared across screens performed with the same library, when JACKS outperforms MAGeCK (multi-screen joint analysis, libraries with broad efficacy variance) and when it does not (single screen, novel libraries with no prior efficacy), library-reuse efficacy transfer, downstream essentiality interpretation, and the 2.5x sample-size reduction enabled by efficacy-aware testing. Use when running multiple screens with the same library, when guide-level noise is suspected to dominate per-gene signal, when reusing published essentiality reference screens for efficacy priors, or when comparing screens performed across cell lines that share library but differ biologically.

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Project gene annotations across genomes using TOGA (Kirilenko 2023 whole-genome-alignment chain-based projection with intactness classification), CESAR 2.0 (Sharma, Schwede & Hiller 2017 codon-aware exon projection), LiftOff (Shumate & Salzberg 2021 reference-based annotation transfer), Liftover (UCSC), GeMoMa (Keilwagen 2019 evidence-based projection), and Comparative Annotation Toolkit (CAT). Use when transferring annotations from a well-annotated reference to query genome(s), classifying gene-loss vs gene-intact across many genomes at scale, building Zoonomia-style comparative annotations across hundreds of mammals or birds (Kirilenko 2023), detecting pseudogenization, projecting alternative isoforms, or selecting between WGA-anchored (TOGA) vs ortholog-based (LiftOff) annotation transfer strategies.

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Performs gene-level association from GWAS summary statistics via genetically predicted tissue expression using FUSION, PrediXcan, S-PrediXcan, S-MultiXcan, UTMOST, MOSTWAS, kTWAS, EpiXcan, TIGAR-V2, and probabilistic fine-mapping with FOCUS and MA-FOCUS. Use when running TWAS from GWAS sumstats, prioritising candidate causal genes from a GWAS lead locus, picking single-tissue vs cross-tissue models, identifying LD-induced TWAS false positives, choosing ancestry-matched prediction weights, fine-mapping co-regulated TWAS hits, or triangulating TWAS with cis-eQTL Mendelian randomization and colocalization to nominate a causal gene.

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Performs subgroup and heterogeneous treatment effect (HTE) analyses for clinical trials. Covers Mantel-Haenszel pooling, Breslow-Day, interaction tests in regression, RERI for additive interaction, modern data-adaptive HTE methods (STEPP, SIDES, causal forests, X/R-learners), Bayesian shrinkage (Dixon-Simon, MAP, EXNEX), graphical multiplicity (Bretz-Maurer), and credibility frameworks (Sun BMJ, EMA 2019). Use when analyzing treatment effects across patient subgroups for regulatory submissions or precision-medicine claims.

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