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Assemble a short-form 'vignette' ad from clean product cutouts composited over a kinetic background video — birefnet cutout, then a cold-open text card + product carousel + annotated specimen-sheet end card, plus loudnorm + separate-pass music mux. FREE assembly (PIL + rsvg + FFmpeg); the recipe supplies the config and gates the paid BG/cutout/music calls to their own capabilities. Use for the vignette format.

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Reverse-engineer a company's sales and marketing tech stack from public signals. Detects CRMs, cold email tools, people databases, ad pixels, email delivery services, and outbound sending domains via DNS records, website source inspection, Apify technology profiling, blacklist checks, and public spam complaint searches. Works on single companies or batches. Outputs a structured markdown report per company.

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Extract visual branding (colors, typography, layout patterns) from a client's website and generate a style preset compatible with the HTML slides skill and a brand config JSON for the content asset creator. Uses WebFetch to read pages and analyzes CSS/HTML to identify the color palette, font pairings, and aesthetic patterns.

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Build the deterministic PIL pill overlays for a 'perfect-score + proof-points' UGC video ad (white 10/10 score header with a medal + orange sub with a finger-down + 3-4 green-check proof pills) and composite them onto a base clip in a diagonal L->R->L->R cascade, then mux the music bed into master-final.mp4. Config-driven (config.json), 1080x1920 9:16, FREE and deterministic (no paid calls, text stays pixel-crisp). Use for the overlay-proof-points format; the base clip + music come from separate paid capabilities.

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Assemble a 3D-CGI app sizzle — nano-banana CGI plates (blank-glow floating phone + a CGI studio with rim-light + placeholder burst shapes; studio look and phone finish come from the recipe choices) plus PIL compositing of the REAL App Store screenshots onto the bezel + burst-out overlays, driven by Kling 3.0 i2v steady-float per beat with a per-beat Ken-Burns FFmpeg push-in fallback when Kling garbles the UI, then VO/music mix (sidechain duck, loudnorm) + 1.15x speed + anti-AI grain finalize. The on-screen UI, any faces in it, and the wordmark are ALWAYS real assets composited via PIL — never AI-rendered. The paid steps (plates, i2v clips, VO, music) are separate capabilities; this ships the config + PIPELINE + FREE assembly and the recipe orchestrates the spend. Use for the cgi-app-sizzle video format.

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Assemble a cinematic live-action-style music-video ad from a config — an original sung anthem carries the whole narrative while N 35mm-film-look i2v clips are each cut to their lyric window and hard-concatenated on the beat as a 3-act arc, the anthem muxed at loudnorm I=-14, cinematic lower-third serif captions built from the song's OWN word timings (never Whisper) with the hook line landing on the chorus drop, and closed on a brand end card composited from the real asset — never AI-rendered text. This is the FREE deterministic assembly stage (cut-to-window + hard concat + anthem mux + captions + end card); the anthem, keyframes, and clips come from create-music-elevenlabs / create-image-gpt-image-fal / create-video-fal. Use for the cinematic-music-video format.

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Render a punchy ~12s vertical (9:16) music-only direct-response OFFER ad as a 4-beat kinetic-typography film — HEADLINE slam → real PRODUCT drop → CLAIM/proof → CTA pill — from one config of copy slots, a real product photo, a brand palette, fonts, bpm, and beat split. DETERMINISTIC + FREE (a bundled Remotion project; springs + interpolate, no AI-gen for visuals). Backgrounds are engine gradient divs off the palette, props are inline SVG, the ONLY composited bitmap is the REAL product photo (objectFit:contain, never stretched), and ALL headline/claim/CTA/URL/wordmark text is typeset in the engine — never AI-rendered (the format's credibility guard). A driver binds the config to Remotion input props, renders the 9:16 master, and derives a 1:1 center-crop with ffmpeg. Two gating checks run before render (claim verbs must match the product's physical format; the claim beat needs an edge-entry mechanism prop). Use for the motion-graphics-offer-ad format.

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Assemble a song-driven music-video ad from a config — a generated sung track carries the whole narration across N tableaux (one keyframe -> one i2v clip per lyric beat) with NO separate voiceover, captions synced to the song's OWN word timings (script-window, never Whisper) and the hook word landing on the chorus drop, closed on a PIL brand end card. This is the FREE deterministic assembly stage (clip cut-to-timeline + captions + end card + FFmpeg composite); the song, keyframes, and clips come from create-music-elevenlabs / create-image-fal / create-video-fal. Use for the song-driven-music-video format.

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Composite a generated creator track into a product layer, per beat — in the creator's zone on split beats (either side of a seam), full frame on creator beats, hidden on product beats — with the creator's voice as the audio (loudness -14 LUFS) and an optional ducked music bed, or a music-only or silent master for voiceless formats. Free, local ffmpeg. Pairs with footage-cutlist (the layer) and create-creator-takes-h3 (the creator). Use for split-screen (with an optional zoom and brand-colour seam bar), screen-insert and any creator-over-product format.

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Render a 'brand identity reveal' video from a config — a single poster frame in a real, softly-lit space (real wall, soft-focus plant in the corner, dappled leaf shadow, illuminated poster) whose artwork HARD-CUTS through ~10 on-brand poster mockups (hero product, IG post, hanging banners, sticker sheet, logo lockup, poster, two lifestyle stills, packaging, big icon) then holds on a brand end card. The environment plate is one create-image-fal generation; the mockups are real-DOM HTML frame-stepped via Playwright, perspective-composited into the detected frame quad with the plate's real leaf-shadow multiplied back onto each poster (reads as behind glass), sequenced by FFmpeg. Deterministic assembly, FREE (the plate comes from create-image-fal, the bed from create-music-elevenlabs), music bed only and approved brand copy only. Use for the brand-identity-reveal format.

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Assemble an iMessage notification-cascade video ad (≈14s, 9:16) from a plate of a phone lying face-up (setting chosen by the user) + 3–5 messages — authentic Apple Messages banners composited in PIL (SF Pro text, green Messages icon, warm translucent-greige fill, soft shadow) spring in one-by-one at the BOTTOM and push the stack UP, a right-aligned Show-less/X pill rides above, the X clears the stack, then a serif end card resolves. FREE assembly (PIL + ffmpeg); the recipe supplies the per-brand plate, notifications, and end-card config and gates the paid plate-clean/music calls to their own capabilities. Use for the imessage-notification-cascade format.

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Assemble a creator picture-in-picture product-listicle ad from a config — the creator stays FULL-FRAME the whole beat (voice plus lips generated together per beat, no separate VO, no cut to a full-frame product shot), and on each product beat three persistent overlays ride on top for the WHOLE beat — a title pill top-center, the DEMO in a rounded PiP window top-right (the brand's real UGC clip MUTED, or for a no-UGC brand the product's own autocropped UI still / screen-recording sized to fill the window), and a bottom product card (rounded thumbnail plus 'N · CATEGORY' small-caps plus product NAME in a serif face). Hook plus CTA beats are the creator full-frame with the title pill only. Assembly builds ONE full-1080x1920 transparent overlay PNG per beat, overlays it on the creator clip (cover-scaled to 1080x1920) for the whole beat keeping the native audio, concats all beats, then burns captions LAST as timed PIL PNG overlays (this ffmpeg has no libass) timed deterministically from the known per-beat script. This is the FREE deterministic assembly stage (overlay-PNG build plus cover-scale composite plus concat plus PIL-PNG caption burn); the creator anchor and the N native talking clips come from create-image-fal (Seedream v5 Pro) and create-video-fal (Seedance 2.0). Use for the creator-pip-listicle format.

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Assemble a silent, music-led 3-world product-tour ad — trim and hard-cut-concat the per-world WIDE-arrival + top-down-macro clips, composite the HTML/Playwright brand end card (approved headline + handwritten product labels + arrows) over an AI flat-lay background, and mux one music bed into a 720x1280 web master. FREE, deterministic assembly (Playwright + PIL/HTML + FFmpeg); the recipe supplies the config and gates the paid clip/background/music calls. Use for the multiworld-product-tour format.

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Extract structured data matching a JSON schema from websites. Handles complex nested schemas, arrays, pagination, and validation. Always outputs via formatOutput.

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Navigate e-commerce sites to extract products, pricing, categories, and inventory. Handles pagination, variants, and JS-heavy storefronts.

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Use when writing or editing Simscape Language (.ssc) source files — components, domains, or functions. Triggers include: model a physical component in .ssc; write physical equations for a Simscape block; add or modify physics or parameterization options in a Simscape component; build a composite component. Also use for validating .ssc syntax, fixing Simscape Language errors, or converting governing equations into working Simscape component code. Do NOT use for configuring or connecting existing Simscape blocks in a Simulink model — that is model building, not language authoring.

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Calls significant loops from protein-directed and targeted 3C assays (HiChIP, PLAC-seq, Capture Hi-C/PCHi-C, ChIA-PET) where the contact background is peak-anchored and coverage-biased, so generic Hi-C loop callers (cooltools dots, Juicer HiCCUPS) use the wrong null. Covers FitHiChIP (config-driven coverage+distance-decay spline regression, peak-to-peak vs peak-to-all foreground, loose vs stringent background, coverage vs ICE bias), MAPS (positive Poisson regression on bias factors for PLAC-seq/HiChIP), hichipper (restriction-site-distance bias model + library QC), CHiCAGO (Delaporte two-component Brownian+technical background for asymmetric bait x other-end Capture Hi-C), the with/without separate-ChIP anchor decision, and differential loops via diffloop. Use when calling loops from HiChIP/PLAC-seq/Capture Hi-C, choosing FitHiChIP/MAPS/CHiCAGO, picking peak-to-all vs peak-to-peak, setting the loop FDR, supplying ChIP peaks as anchors, QCing a HiChIP library, or comparing loops between conditions.

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Designs pegRNAs and nicking guides for prime editing (PE) -- choosing the nick/strand, tuning the primer-binding site (PBS) and reverse-transcription template (RTT) as a per-locus panel, selecting the PE system (PE2/PE3/PE3b/PE4/PE5/PEmax/PE7), adding MMR-evading and PAM-disrupting silent edits, appending epegRNA 3' motifs (tevopreQ1/mpknot), and ranking with PRIDICT/DeepPrime. Covers twinPE/PASTE for large insertions and the prime-vs-base-editing decision. Use when designing a scarless point mutation, small insertion/deletion, or any of the 12 base conversions without a double-strand break, when efficiency is low and MMR inhibition or pegRNA stabilization is needed, or when routing a large insertion to an integrase method. Generic guide scoring and base editing are separate skills.

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Orders and orients assembled contigs into chromosome-scale scaffolds from long-range linking data, inserting N-gap spacers (adds no sequence). Covers Hi-C/Omni-C scaffolding (YaHS, SALSA2, 3D-DNA/Juicer), Hi-C read-mapping prerequisites (map each end separately, no mate rescue, dedup, enzyme-aware), reading the contact map for misjoins/inversions/false-duplications, manual curation in Juicebox/PretextView (the VGP/DToL standard), reference-guided scaffolding (RagTag) and its karyotype-erasure hazard, genetic-map (ALLMAPS) and Bionano optical-map integration, chimera-breaking before scaffolding, gap-filling, and telomere/contig-vs-scaffold-N50 QC (tidk). Use when turning contigs into chromosomes with Hi-C, integrating a linkage map or optical map, choosing a scaffolder by available linking data, or judging whether a chromosome-scale assembly is trustworthy.

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Detects focal chromatin loops (point interactions / corner-dots) in balanced Hi-C and Micro-C contact maps and aggregates/validates a loop set. Covers de-novo calling with cooltools dots (HiCCUPS-style 4-background local enrichment with lambda-chunked FDR), chromosight (template-correlation), and Mustache (scale-space blob detection); aggregate peak analysis (APA) via cooltools pileup for confirmation; the depth/resolution prerequisite (de-novo needs ~5-10kb resolution = hundreds of millions to billions of valid pairs); consensus across callers and convergent-CTCF support as validation; and differential loops via union anchors plus chromosight quantify. Use when calling chromatin loops or dots from a cooler, deciding whether a map is deep enough to call de-novo vs running APA on known CTCF/cohesin anchors, building an aggregate peak pileup, comparing loops across conditions, or validating loop calls. For HiChIP/PLAC-seq/PCHi-C protein-anchored data use FitHiChIP/MAPS, not dots.

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Designs and ranks guide RNAs (sgRNAs) for CRISPR-Cas9/Cas12a gene knockout by scanning a target for PAM sites (NGG SpCas9, NNGRRT SaCas9, TTTV Cas12a, NG SpCas9-NG, near-PAMless SpRY), enumerating candidate spacers, applying hard filters (Pol-III TTTT terminator, 5' G, GC), ranking on-target activity with the context-appropriate model (Rule Set 2/Azimuth for U6/lentiviral, CRISPRscan for T7/embryo, DeepHF for high-fidelity variants, DeepCpf1 for Cas12a), and predicting the indel/frameshift outcome (Bae out-of-frame score, inDelphi, FORECasT, Lindel). Use when selecting sgRNAs to knock out a gene, choosing a nuclease/PAM for a constrained locus, picking which exon to target, or shortlisting guides before an off-target check. Off-target specificity, base/prime editing, and HDR donors are separate skills.

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Designs cytosine (CBE, C-to-T) and adenine (ABE, A-to-G) base-editor guides by positioning the target base at the activity-peak of the editing window (protospacer positions ~5-7, PAM-distal numbering), minimizing bystander edits for product purity, reading dinucleotide context (APOBEC1 TC favored / GC disfavored), and selecting the editor variant (BE4max, ABEmax, ABE8e, YE1/SECURE, TadCBE, CGBE, SpG/SpRY-BE). Covers knockout by premature stop (CRISPR-STOP/iSTOP) and splice-site disruption, the three off-target classes (Cas-dependent, Cas-independent DNA, RNA), outcome prediction (BE-Hive/DeepBE), and the base-vs-prime-vs-HDR decision. Use when installing a transition mutation without a double-strand break, knocking out a gene without indels, or choosing CBE vs ABE. Generic guide scoring, prime editing, and HDR donors are separate skills.

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Processes eDNA metabarcoding from raw paired-end reads to species tables, navigating ASV (DADA2, UNOISE3) vs OTU (swarm v2) decision (Callahan 2017 vs Schloss multi-copy-16S critique), marker/primer choice (Leray COI, MiFish 12S, 515F/806R 16S, ITS2) with primer-specific bias, OBITools3 v3 command-name break (obi stats plural; .tar.gz taxonomy), tag-jumping with dual-indexing (Schnell 2015; NovaSeq 10x MiSeq), decontam as screening-not-classifier (Davis 2018), read-counts-not-abundance critique (Lamb 2019), site-occupancy modeling (Ficetola 2015), Naive-Bayes calibration limits (Bokulich 2018), and eDNA decay (Strickler 2015). Use when going from raw eDNA FASTQ to species tables, picking marker + denoising pipeline, deciding whether read counts represent abundance, applying occupancy modeling, configuring OBITools3 v3, or interpreting decontam output. Not for clinical 16S microbiome (see microbiome/amplicon-processing).

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Bead-based signal normalization and cross-batch harmonization for CyTOF and high-parameter cytometry - EQ four-element bead normalization of instrument sensitivity drift (CATALYST normCytof, premessa), and reference-anchor cross-batch normalization (CytoNorm, per-cluster quantile splines). Covers the distinction between within-run drift correction and between-batch correction, the mandatory anchor/reference sample, why normalization is per-cluster with many quantiles, and the over-correction risk. Use when correcting CyTOF signal drift, harmonizing multi-batch or multi-site studies, or deciding whether to normalize data versus model batch in the design.

日本語の概要は準備中です。原文の説明を表示しています。