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概要と使いどころ
Patterns for building entity actors with Akka.Hosting - GenericChildPerEntityParent, message extractors, cluster sharding abstraction, akka-reminders, and ITimeProvider. Supports both local testing and clustered production modes.
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Write unit and integration tests for Akka.NET actors using modern Akka.Hosting.TestKit patterns. Covers dependency injection, TestProbes, persistence testing, and actor interaction verification. Includes guidance on when to use traditional TestKit.
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Guidelines for making .NET libraries and applications trimming-safe and Native AOT compatible. Covers the trimming/AOT model, the MSBuild properties that enable analysis (IsTrimmable, IsAotCompatible, PublishTrimmed, PublishAot), the trimming attributes (RequiresUnreferencedCode, RequiresDynamicCode, DynamicallyAccessedMembers, UnconditionalSuppressMessage), IL2xxx/IL3xxx warning codes, and a pattern playbook: source generators and UnsafeAccessor, generated-interception diagnostic suppressors, intentional runtime scanning boundaries, trimming-safe islands and feature switches, migration analyzers, and temporary warning-approval baselines. Use when introducing trimming/AOT support, resolving IL2xxx/IL3xxx warnings, or making reflection-heavy code trimming-safe in C# / .NET codebases. For System.Text.Json AOT scenarios, see also the serialization skill.
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Configure Akka.NET with .NET Aspire for local development and production deployments. Covers actor system setup, clustering, persistence, Akka.Management integration, and Aspire orchestration patterns.
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Handles BED-format genomic intervals (BED3 through BED12, narrowPeak/broadPeak) and the coordinate-system substrate the whole interval category rests on, with bedtools (CLI) and pybedtools/pyranges/pandas (Python). Covers the 0-based half-open vs 1-based-closed convention boundary and the start-1/end-unchanged conversion, the silent failures (chrom-name mismatch, CRLF, lexicographic-vs-version sort under -sorted), genome/chrom.sizes generation, sorting contracts, BED12 block invariants, validation, makewindows, cross-assembly liftover (liftOver/CrossMap), and BED<->VCF/BAM/FASTA conversion. Use when reading, creating, validating, sorting, lifting between genome builds, or converting interval files, preparing inputs for bedtools/tabix/bigBed, or debugging an off-by-one or empty-overlap result.
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Extracts cfDNA fragmentomics features (DELFI genome-wide short/long ratios, WPS nucleosome positioning, Griffin GC-corrected accessibility profiles, end-motifs/MDS, OCF) for cancer detection and tissue-of-origin from plasma WGS. Centers on the nuclease-footprint reframe (every feature re-reads one nucleosome object), the mandatory GC correction, and the cross-protocol non-comparability that breaks naive classifiers. Runs FinaleToolkit (real CLI/Python, MIT) and the Griffin Snakemake pipeline; DELFI is a method, not a package. Use when deriving fragment-based signal from cfDNA, choosing a feature family for detection vs subtyping, or diagnosing why a fragmentomic model failed validation.
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Designs cytosine (CBE, C-to-T) and adenine (ABE, A-to-G) base-editor guides by positioning the target base at the activity-peak of the editing window (protospacer positions ~5-7, PAM-distal numbering), minimizing bystander edits for product purity, reading dinucleotide context (APOBEC1 TC favored / GC disfavored), and selecting the editor variant (BE4max, ABEmax, ABE8e, YE1/SECURE, TadCBE, CGBE, SpG/SpRY-BE). Covers knockout by premature stop (CRISPR-STOP/iSTOP) and splice-site disruption, the three off-target classes (Cas-dependent, Cas-independent DNA, RNA), outcome prediction (BE-Hive/DeepBE), and the base-vs-prime-vs-HDR decision. Use when installing a transition mutation without a double-strand break, knocking out a gene without indels, or choosing CBE vs ABE. Generic guide scoring, prime editing, and HDR donors are separate skills.
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Visualises RNA-modification data with transcript-feature metagene plots (Guitar GuitarPlot; MetaPlotR; deepTools computeMatrix scale-regions), peak-centred heatmaps (ComplexHeatmap; deepTools plotHeatmap), IP-vs-input paired browser tracks (log2 IP/input bigWig via deepTools bamCompare; pyGenomeTracks; Gviz; IGV/UCSC track hubs), DRACH sequence-logo plots (ggseqlogo; MEME), feature-distribution stacked bars, and volcano/MA plots for differential modification. Establishes stop-codon enrichment in the metagene plot as the biological QC anchor for any MeRIP dataset (Dominissini 2012; Meyer 2012). Use when producing the canonical metagene plot with stop-codon enrichment as a QC anchor, building paired IP/input genome-browser tracks at single-locus resolution, plotting peak-centred heatmaps clustered by condition, summarising peak distribution across transcript features, generating DRACH motif logos as sanity checks, rendering volcano plots of differential m6A, or reproducing the stop-codon enrichment plot.
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Build local BLAST databases and run searches using NCBI BLAST+ command-line tools. Use when running >50 queries, building custom databases with -parse_seqids and -taxid, downloading prebuilt NCBI databases via update_blastdb.pl, choosing -task variants (megablast/dc-megablast/blastn/blastn-short), tuning soft/hard masking, scaling threads, or extracting hits with blastdbcmd. Encodes BLAST v5 vs v4 database format, taxonomy filtering, makeblastdb pitfalls.
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Identify differentially bound regions across CLIP-seq conditions (knockdown vs control, treatment vs vehicle, disease vs healthy) using DEWSeq (sliding-window DESeq2), Flipper (Skipper-downstream), ASpeak, edgeR, or limma-voom. Use when computing condition-level changes in RBP binding intensity, choosing peak-level vs window-level vs crosslink-level testing, designing replicate experiments, or distinguishing biological binding shifts from technical confounders.
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Analyzes CUT&RUN (Skene Henikoff 2017) and CUT&Tag (Kaya-Okur 2019) chromatin profiling data. Handles SEACR vs MACS2 peak calling (with the btaf375 2025 benchmark guidance), pA-MNase vs pA-Tn5 vs pAG-Tn5 chimera differences, E. coli spike-in carryover normalization, IgG-only control logic (no input), characteristic fragment-size signatures (25-75 bp for CUT&Tag), and lower depth requirements (5M reads typical vs 25M for ChIP). Use when calling peaks from CUT&RUN/CUT&Tag, scaling by E. coli spike-in carryover, choosing SEACR norm mode, or comparing CUT&RUN/Tag results to traditional ChIP.
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Calls ChIP-seq peaks with MACS3, MACS2, HOMER, or SPP across narrow (TF) and broad (histone) modes. Handles input control matching, fragment-size modeling vs --nomodel, effective genome size, ENCODE-style IDR vs naive overlap, hyper-ChIPable artifacts, and aligner-specific shifts. Use when calling peaks from ChIP-seq alignments, choosing between narrow vs broad mode for a histone mark, deciding model vs nomodel for low-depth data, applying ENCODE pseudoreplicate IDR, or reconciling MACS vs HOMER vs SPP results.
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Discovers de novo motifs and tests known motif enrichment in ChIP-seq, ATAC-seq, or other peak sequences using HOMER, MEME-ChIP (STREME, CentriMo, TOMTOM, FIMO), monaLisa, and AME. Handles background selection (GC-matched, dinucleotide-shuffled, Markov order-2, peak-flanks), motif databases (JASPAR 2024 CORE PWMs, JASPAR 2026 deep-learning collection, HOCOMOCO v12, HOMER built-in), centrally-enriched motif testing, and differential motif analysis. Use when identifying TF binding motifs in peaks, testing for known TF enrichment, scanning for motif instances, comparing motif content between conditions, or interpreting motifs from deep learning models.
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Call accessible chromatin regions from ATAC-seq BAM files using MACS3, MACS2, Genrich, or HMMRATAC. Use when identifying open chromatin from aligned ATAC-seq, choosing between point-source vs HMM peak callers, applying ENCODE-style pseudoreplicate IDR, removing blacklist regions, or fixing 501bp consensus peaks for downstream differential analysis.
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Use when capturing or analyzing an iOS .memgraph, especially when the task mentions a memory leak, heap growth, persistent memory increase, ownership path, or matched-capture comparison with Apple CLI tools. Covers unambiguous Simulator capture, leaks/heap/vmmap/malloc_history evidence, raw artifact preservation, and same-flow verification. Use debugging-instruments for interactive Xcode Memory Graph, Instruments, generic retain-cycle inspection, or LLDB work.
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This skill should be used when user asks to "improve my mAP", "why is my model overfitting", "my training is diverging", "read my results.csv", "interpret my training curves", "my AP50 is good but AP50-95 is bad", "my recall is low", "how do I pick learning rate", "which augmentations should I use", "should I use a bigger model", "tune hyperparameters", or asks how to train YOLO26 for detection, instance or semantic segmentation, pose, OBB, classification, or depth.
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This skill should be used when user asks to "set up overleaf", "configure overleaf cookie", "overleaf auth failed", "overleaf 401", "overleaf session expired", "overleaf unauthorized", or needs to install or refresh their Overleaf session cookie for the overleaf-skills plugin.
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Generate read-only MongoDB queries (find) or aggregation pipelines using natural language, with collection schema context and sample documents. Use this skill whenever the user asks to write, create, or generate MongoDB queries, wants to filter/query/aggregate data in MongoDB, asks "how do I query...", needs help with query syntax, or discusses finding/filtering/grouping MongoDB documents. Also use for translating SQL-like requests to MongoDB syntax. Does NOT handle Atlas Search ($search operator), vector/semantic search ($vectorSearch operator), fuzzy matching, autocomplete indexes, or relevance scoring - use search-and-ai for those. Does NOT analyze or optimize existing queries - use mongodb-query-optimizer for that. Does NOT handle aggregation pipelines that involve write operations. Requires MongoDB MCP server.
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This skill should be used when the user asks to "create openship.json", "configure an OpenShip deployment", "make a repo deployable on OpenShip", or fix "openship config validate" errors.
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Use this skill when the user asks what information a Stripe Connect connected account must provide for verification, onboarding, KYC, or account requirements; when they need to compare requirements between connected-account setups; or when they ask which verification fields, documents, or business details are required for a particular platform country, account country, business type, dashboard, service agreement, or capability.
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Use this skill any time a .pptx or .potx file is involved in any way — as input, output, or both. This includes: creating slide decks, pitch decks, or presentations; reading, parsing, or extracting text from any .pptx or .potx file (even if the extracted content will be used elsewhere, like in an email or summary); editing, modifying, or updating existing presentations; combining or splitting slide files; working with templates (.potx), layouts, speaker notes, or comments. Trigger whenever the user mentions "deck," "slides," "presentation," or references a .pptx or .potx filename, regardless of what they plan to do with the content afterward. If a .pptx or .potx file needs to be opened, created, or touched, use this skill.
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This skill should be used when user asks to "query Azure resources", "list storage accounts", "manage Key Vault secrets", "work with Cosmos DB", "check AKS clusters", "use Azure MCP", or interact with any Azure service.
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This skill should be used when user asks to "create a PR", "make a pull request", "open PR for this branch", "submit changes as PR", "push and create PR", or explicitly invokes "create-pr".
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Build, migrate, and deploy Next.js apps on Cloudflare Workers with vinext. Use when starting a Next.js project on Cloudflare, moving an existing app to Workers, choosing between vinext and OpenNext, or setting up vinext for Workers. For setup, migration, or deployment, install vinext's upstream skills with `npx skills add cloudflare/vinext` if missing, then read and follow the applicable skill and docs.
日本語の概要は準備中です。原文の説明を表示しています。