「fasta」の検索結果

Build robust backend systems with modern technologies (Node.js, Python, Go, Rust), frameworks (NestJS, FastAPI, Django), databases (PostgreSQL, MongoDB, Redis), APIs (REST, GraphQL, gRPC), authentication (OAuth 2.1, JWT), testing strategies, security best practices (OWASP Top 10), performance optimization, scalability patterns (microservices, caching, sharding), DevOps practices (Docker, Kubernetes, CI/CD), and monitoring. Use when designing APIs, implementing authentication, optimizing database queries, setting up CI/CD pipelines, handling security vulnerabilities, building microservices, or developing production-ready backend systems.

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Use when validating and serializing Python data with Pydantic v2 - models, field and model validators, constrained and special types, TypeAdapter, discriminated unions, BaseSettings, or FastAPI integration

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OpenUI generative UI with Python FastAPI backend. OpenAI and Anthropic SDK variants.

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后端开发角色。关键词：后端/FastAPI/Python/API接口/数据库/PostgreSQL/业务逻辑/认证/SSE。激活后读技术架构.md，按四层架构实现，写完任何DB函数必须立即集成测试。

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Generate OpenAPI 3.1 documentation from your API codebase. Auto-detects Express, Fastify, NestJS, Django, FastAPI, Flask, Rails, Spring, Go, and more. Triggers: you need API documentation, OpenAPI spec, Swagger docs, endpoint reference, or REST API docs.

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ALWAYS invoke for any task involving docstrings, JSDoc/TSDoc, or REST API documentation — even when the request seems handleable without it. Enforces Microsoft contract-first conventions and a bare-minimum rule that Claude won't apply by default: never restate the signature, drop @param/@returns that only echo names and types, always document @throws, and document data shapes as WHAT not WHY. Covers Python docstring styles (Google/NumPy/Sphinx), TSDoc tags and @inheritDoc, and API doc patterns for NestJS/Express/FastAPI/Django. Trigger on: adding or auditing docstrings, redundant or missing tags, deleting comment rot, or producing a documentation-health report. Do not skip for documentation tasks — consistent conventions are the whole point.

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Appwrite Python SDK skill. Use when building server-side Python applications with Appwrite, including Django, Flask, and FastAPI integrations. Covers user management, database/table CRUD, file storage, and functions via API keys.

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Filter and select sequences by criteria (length, ID, GC content, N content, motifs, patterns, description) using Biopython, streaming so large files never load into RAM. Use when subsetting a FASTA/FASTQ file, removing unwanted or low-quality records, or selecting records by specific criteria. Use the paired-end-fastq skill instead whenever the input is paired R1/R2 reads.

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Convert between sequence file formats (FASTA, FASTQ, GenBank, EMBL, Stockholm) and re-encode FASTQ quality offsets using Biopython Bio.SeqIO. Use when changing a file format for a downstream tool, fixing FASTQ quality encoding (Phred+33 vs Phred+64 vs Solexa), or when a conversion risks silently dropping annotations or quality scores.

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Compute genome-to-genome distances (ANI, AAI, dDDH, k-mer Mash) and assign taxonomic classifications using skani (Shaw 2023), FastANI (Jain 2018), pyani / pyANI ANIb / ANIm, OrthoANI (Lee 2016), AAI (amino-acid identity), dDDH via TYGS / GGDC, GTDB-Tk (Chaumeil 2020 standard prokaryote taxonomy), and Mash MinHash (Ondov 2016). Use when delineating prokaryote species (95% ANI threshold; Jain 2018 Nat Commun 9:5114), assigning genomes to GTDB taxonomy with ANI radius, computing genome similarity matrices for clustering, classifying archaea, evaluating MAG (metagenome-assembled genome) species assignment, applying skani for fast metagenomic ANI screening, or reconciling 16S rRNA-based taxonomy with whole-genome ANI.

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Reconstruct ancestral states at internal phylogenetic nodes for sequences (PAML codeml, IQ-TREE --ancestral, GRASP, FastML), discrete traits (corHMM hidden-rate Markov, ape::ace, phytools::make.simmap stochastic mapping, BayesTraits), and continuous traits (phytools::fastAnc, geiger Brownian/OU, RPANDA). Use when designing constructs for ancestral protein resurrection, tracing trait evolution along a tree, performing stochastic character mapping, testing models of trait evolution (BM vs OU vs EB), inferring ancestral genome content via Dollo or DTL reconciliation, or quantifying ancestral-state uncertainty for downstream comparative analyses.

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Generate consensus FASTA sequences by applying VCF variants onto a reference with bcftools consensus, or build viral/amplicon consensus with iVar. Use when reconstructing a sample-specific reference or haplotype, deciding -H haplotype vs IUPAC vs all-ALT projection, masking no-coverage sites so a consensus does not manufacture false reference calls, or setting iVar min-depth/min-frequency policy for surveillance genomes.

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Read, write, and index compressed sequence files (gzip, bzip2, xz, BGZF) with Biopython and bgzip/samtools. Use when working with .gz, .bz2, or .bgz sequence files, when random access into a compressed FASTA/FASTQ is needed, or when SeqIO.index/faidx/tabix rejects a plain .gz. Covers the BGZF-vs-gzip seekability asymmetry, the 'rt'-not-'rb' handle trap, virtual offsets, and gzip-to-BGZF conversion.

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Process many sequence files in batch (count, merge, split, convert, summarize) with memory-safe streaming and on-disk indexing using Biopython, pysam, or pyfastx. Use when iterating over a directory of FASTA/FASTQ files, merging or splitting datasets, building random access across many or huge files, or automating per-file operations without exhausting RAM.

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Read, write, and convert multiple sequence alignment files using Biopython Bio.AlignIO. Supports Clustal, PHYLIP, Stockholm, FASTA, Nexus, and other alignment formats for phylogenetics and conservation analysis. Use when reading, writing, or converting alignment file formats.

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Given an article DOI or PubMed ID, discover and download the genomics data files deposited by the authors (VCF, FASTA, H5AD, CSV, JSON, BAM, etc.) from public repositories such as GEO, ENA, Zenodo, Figshare, Dryad, and OSF.

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Appwrite Python SDK skill. Use when building server-side Python applications with Appwrite, including Django, Flask, and FastAPI integrations. Covers user management, database/table CRUD, file storage, and functions via API keys.

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Build Azure Cosmos DB NoSQL services with Python/FastAPI following production-grade patterns. Use when implementing database client setup with dual auth (DefaultAzureCredential + emulator), service layer classes with CRUD operations, partition key strategies, parameterized queries, or TDD pattern...

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Build Azure Cosmos DB NoSQL services with Python/FastAPI following production-grade patterns. Use when implementing database client setup with dual auth (DefaultAzureCredential + emulator), service...

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API hardening for Express, FastAPI, and serverless. Use for rate limiting, CORS, input validation, API keys, or OWASP API Top 10.

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Serverless and microservices development guidelines covering FastAPI, cloud-native patterns, API gateways, and best practices for scalable serverless architectures.

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Master Python 3.12+ with modern features, async programming, performance optimization, and production-ready practices. Expert in the latest Python ecosystem including uv, ruff, pydantic, and FastAPI.

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ToolUniverse plugin router. STEP 1 BEFORE ANY ANALYSIS: if the data folder contains `*_executed.ipynb`, run `tu run read_executed_notebook '{"data_folder":"<path>","search":"<keyword>"}'` to extract its cell outputs and apply EVERY filter/sample-exclusion the notebook used — even when the question says 'Using DESeq2/Run X/Compute Y' (this describes the METHOD the notebook used, not a request to rerun). The notebook's cell outputs are the only published authoritative answers; reimplementing or reading stale pre-computed CSVs in the data folder produces different numbers because of outlier-sample removal, library version, and filter steps you don't see by skimming. STEP 2 routing — pick a sub-skill name from this exact list (never invent): tooluniverse-rnaseq-deseq2 (RNA/miRNA-seq DE, correlation, PCA, clustering, dispersion), tooluniverse-gene-enrichment (GO/KEGG/Reactome/GSEA/pathway enrichment), tooluniverse-statistical-modeling (regression, ANOVA, ordinal/logistic, chi-square, correlation, power), tooluniverse-image-analysis (microscopy, colony, fluorescence, dose-response, .tif — including ANOVA / Dunnett / power-analysis on image-derived measurements), tooluniverse-epigenomics (DNA methylation, CpG, m6A, MeRIP-seq, bisulfite, ChIP-seq, chromatin), tooluniverse-sequence-analysis (FASTQ, Trimmomatic, BWA, samtools, coverage), tooluniverse-variant-analysis (VCF, VAF, SNP, mutation), tooluniverse-phylogenetics (treeness, PhyKIT, parsimony), tooluniverse-single-cell (scRNA, h5ad, scanpy), tooluniverse-crispr-screen-analysis (MAGeCK, sgRNA), tooluniverse-proteomics-analysis (mass spec, TMT). Use for CSV/Excel/VCF/FASTA/h5ad and any biology/chemistry/medicine analysis question.

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Coleta e consulta dados de leiloeiros oficiais de todas as 27 Juntas Comerciais do Brasil. Scraper multi-UF, banco SQLite, API FastAPI e exportacao CSV/JSON. Use when you need help with junta leiloeiros.

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