「pam」の検索結果

Design and harden Roblox client/server networking with RemoteEvent, RemoteFunction, and UnreliableRemoteEvent; server authority, argument and Instance validation, rate limits, proximity/ownership checks, targeted replication, streaming, lifecycle, prediction, and reconciliation. Use for Roblox remotes, exploits, request spam, multiplayer replication, network ownership, high-frequency cosmetic updates, or server/client desynchronization.

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Find and remove AI slop — the generic, machine-default visual and copy tics of vibe-coded products — from a web project. Use when the user asks to "kill AI slop", "de-slop", "remove the AI look", "make this not look AI-generated", or clean up a landing page / UI / docs that feels templated. Detects and fixes the catalogue of tells: indigo→violet gradients, gradient-clip headlines, the default semantic palette, one-hue status boxes, atmospheric gradients, serif-italic emphasis, highlighted keywords, AI copywriting voice ("not just X — it's Y"), emoji everywhere, glowing status dots, wobbling spinners, colored-left-border callouts, pastel icon tiles, glassmorphism, over-rounding, oversized shadows, borders that die at corners, badge & pill spam, AI-drawn SVG icons, kickers over every heading, flat type hierarchies, invented stat rows, 01/02/03 section markers, cards nested in cards, the default Inter/Space Grotesk look, and more. Works on HTML/CSS, React/Vue/Svelte/Astro, Tailwind, PHP, and Markdown copy.

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Reverse-engineer a company's sales and marketing tech stack from public signals. Detects CRMs, cold email tools, people databases, ad pixels, email delivery services, and outbound sending domains via DNS records, website source inspection, Apify technology profiling, blacklist checks, and public spam complaint searches. Works on single companies or batches. Outputs a structured markdown report per company.

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Designs donor/repair templates for precise CRISPR knock-ins -- choosing the format (ssODN, long-ssDNA/Easi-CRISPR, dsDNA/plasmid, AAV6), sizing homology arms, placing the cut within ~10 bp of the edit, and adding a mandatory codon-checked blocking (PAM/seed) mutation so the edited allele is not re-cut. Frames the HDR-vs-NHEJ-vs-MMEJ pathway competition, the MMEJ (PITCh) and homology-independent (HITI/HMEJ) alternatives for post-mitotic cells, ssODN strand/asymmetry choice, phosphorothioate end-protection, and ranked HDR enhancers. Use when designing a donor for a point mutation, epitope/fluorophore tag, allele replacement, or knock-in, or when HDR efficiency is low. Guide design and base/prime editing are separate skills.

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Designs cytosine (CBE, C-to-T) and adenine (ABE, A-to-G) base-editor guides by positioning the target base at the activity-peak of the editing window (protospacer positions ~5-7, PAM-distal numbering), minimizing bystander edits for product purity, reading dinucleotide context (APOBEC1 TC favored / GC disfavored), and selecting the editor variant (BE4max, ABEmax, ABE8e, YE1/SECURE, TadCBE, CGBE, SpG/SpRY-BE). Covers knockout by premature stop (CRISPR-STOP/iSTOP) and splice-site disruption, the three off-target classes (Cas-dependent, Cas-independent DNA, RNA), outcome prediction (BE-Hive/DeepBE), and the base-vs-prime-vs-HDR decision. Use when installing a transition mutation without a double-strand break, knocking out a gene without indels, or choosing CBE vs ABE. Generic guide scoring, prime editing, and HDR donors are separate skills.

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Designs pegRNAs and nicking guides for prime editing (PE) -- choosing the nick/strand, tuning the primer-binding site (PBS) and reverse-transcription template (RTT) as a per-locus panel, selecting the PE system (PE2/PE3/PE3b/PE4/PE5/PEmax/PE7), adding MMR-evading and PAM-disrupting silent edits, appending epegRNA 3' motifs (tevopreQ1/mpknot), and ranking with PRIDICT/DeepPrime. Covers twinPE/PASTE for large insertions and the prime-vs-base-editing decision. Use when designing a scarless point mutation, small insertion/deletion, or any of the 12 base conversions without a double-strand break, when efficiency is low and MMR inhibition or pegRNA stabilization is needed, or when routing a large insertion to an integrase method. Generic guide scoring and base editing are separate skills.

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Designs pooled sgRNA libraries for CRISPR knockout, interference (CRISPRi), activation (CRISPRa), Cas12a multiplex, base-editor, and prime-editor screens. Covers on-target scoring (Rule Set 2, Azimuth, DeepSpCas9, CRISPRon), off-target scoring (CFD, MIT), TSS-relative positioning for CRISPRi/a (Horlbeck, Dolcetto, Calabrese), PAM-variant chemistries, control-guide composition, oligo cloning architecture, and library QC. Use when choosing a genome-wide library (GeCKOv2 vs Avana vs Brunello vs TKOv3 vs Inzolia), designing a focused or paralog-focused custom library, picking CRISPRi vs CRISPRa TSS windows, deciding control-guide proportions, or diagnosing library skew and dropout in a freshly cloned pool.

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Reconstruct ancestral states at internal phylogenetic nodes for sequences (PAML codeml, IQ-TREE --ancestral, GRASP, FastML), discrete traits (corHMM hidden-rate Markov, ape::ace, phytools::make.simmap stochastic mapping, BayesTraits), and continuous traits (phytools::fastAnc, geiger Brownian/OU, RPANDA). Use when designing constructs for ancestral protein resurrection, tracing trait evolution along a tree, performing stochastic character mapping, testing models of trait evolution (BM vs OU vs EB), inferring ancestral genome content via Dollo or DTL reconciliation, or quantifying ancestral-state uncertainty for downstream comparative analyses.

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Discover RBP binding motifs from CLIP-seq peaks or single-nucleotide crosslink sites using HOMER, MEME/STREME, kpLogo, mCross (CL-position-registered motifs), PEKA (positional k-mer enrichment), RBPamp (affinity), and RNA Bind-n-Seq (RBNS) cross-validation. Use when characterizing RBP sequence specificity, registering motifs to crosslink positions, validating in vivo CLIP motifs against in vitro RBNS Kd, reconciling motif disagreements across tools, or correcting for the uracil crosslinking bias that contaminates raw CLIP motif logos.

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Analyzes base-editing screens for variant function. Covers library design (Hanna 2021 ClinVar-scale CBE screen benchmarked on BRCA1/2, Cuella-Martin 2021 DDR saturation), CBE vs ABE chemistry choice (BE3/BE4 vs ABE7.10/ABE8.20/ABE8e), editing-window math (positions 4-8 from PAM-distal end; 4-7 for ABE7.10), bystander-edit quantification and the variant-call ambiguity it creates, sgRNA-efficiency filtering before hit calling, indel byproduct interpretation, the substitution-vs-indel diagnostic, variant annotation against ClinVar / COSMIC, and the Broad be-validation-pipeline. Use when designing a BE variant screen, choosing CBE vs ABE for a specific edit, interpreting bystander-confounded hits, distinguishing functional signal from indel artifact, integrating CRISPResso2 output with screen scoring, or deciding BE vs PE for SNV installation.

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Trains and applies base-resolution deep learning models on ChIP-seq / ChIP-nexus / CUT&RUN data. Uses BPNet (Avsec 2021 Nat Genet 53:354; soft motif syntax from ChIP-nexus), chromBPNet (Pampari A et al 2024 bioRxiv; bias-factorized base-resolution profiles), EnFormer (Avsec 2021 Nat Methods 18:1196; 196 kb input, ~100 kb effective receptive field), DeepSEA (Zhou 2015; multi-task CNN), and JASPAR 2026 deep-learning collection (1259 BPNet ChIP models). Performs in silico mutagenesis for variant-effect prediction, DeepLIFT/Grad attribution, and TF-MoDISco motif discovery from attribution scores. Use when predicting variant effects on TF binding, discovering soft motif syntax / cooperativity, integrating ChIP-seq with sequence-only predictions, or applying precomputed JASPAR Deep Learning models to new variants.

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Design, select, and analyze waveforms for radar, sonar, and active sensing using the Phased Array System Toolbox. Covers LFM, NLFM, FMCW, phase-coded, CW, stepped FM, custom IQ, ambiguity functions, sidelobe reduction, and Doppler tolerance. Key objects: phased.LinearFMWaveform, phased.NonlinearFMWaveform, phased.CustomFMWaveform, phased.PhaseCodedWaveform, phased.FMCWWaveform, phased.SteppedFMWaveform, phased.MFSKWaveform, phased.RectangularWaveform, nlfmspec2freq, shapespectrum, ambgfun, pambgfun, sidelobelevel, legendreseq, mlseq, radarWaveformGenerator.

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Use on a mailbox export to sort mail into needs reply, updates, promotional, sales and spam — which messages are addressed to the person at all. For a support queue use jev triage.

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Deploy the dispatcher PCF for a third-party `.ppmplugin` control to a Power Platform environment via `pac pcf push`. The dispatcher PCF is the Studio-side control that dispatches the composite key `<name>/<receiver>` over the wrap shell's `SendMessagePlugin` bridge to the control's native module. Verifies deploy prereqs (Node.js 20+ with npm, .NET SDK, and active `pac auth`), then three confirmation gates — publisher prefix (2–8 chars; defaults to `pamext` or the last-used value from `.extension-state.md`), version bump (patch / minor / major / no-bump), target environment URL (from `pac org who`). Builds the PCF if needed, then pushes. Decoupled from /generate-pcf-companion so the engineer can scaffold and customize locally, then deploy when ready. Updates `.extension-state.md` with deployment history (timestamp, env URL, version, prefix used).

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Compile a PAM control's Android Kotlin module into the runtime-loadable DEX for a `.ppmplugin`. Creates a staged Gradle build with the pinned wrapper and `react-android` compile dependency, verifies manifest/module/package alignment and runtime-loading constraints, builds the release AAR, then runs `d8 --min-api 24`. Writes `ppmplugin/staging/android/<PascalName>Plugin.dex` and surfaces actionable Gradle or d8 failures. Requires JDK 17+ and Android SDK Build-Tools 35.0.0; `d8` is located from the SDK and system Gradle is optional. Run after /generate-ppmplugin-manifest and before /assemble-ppmplugin.

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Read the approved PRD.md and generate the native sources for a third-party PAM control (the compiled `.ppmplugin` track) — iOS Obj-C `<Pascal>Module` plus optional system-frameworks podspec, Android Kotlin `<Pascal>Module` with build.gradle, AndroidManifest and ReactPackage, a dev-only private package.json (react + react-native devDeps for the builds), and the committed `./manifest.json` dispatch contract the PCF and build stage both read. No TypeScript INativeExtension layer — the contract is the manifest plus the native modules' dispatch surface. Emits the layout in shared/repo-layout.md and generates substantially complete native code (compiled later by /build-android-binary and /build-ios-binary, not here). Local only — writes files, runs no git and touches no remote or feed. PCF is generated by /generate-pcf-companion; the bundle is built by /generate-ppmplugin.

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Assemble the final `.ppmplugin` binary bundle for a PAM native extension and verify its contents. First **reconciles** the manifest's declared `entrypoints` against the binaries actually staged — if the manifest declares a platform with no built binary it gates (build it / ship without it / stop) rather than shipping a broken bundle. Then re-runs the plugin's upload-compatibility checks on the reconciled manifest, zips the manifest plus whichever of `android/<PascalName>Plugin.dex` and `ios/<PascalName>Plugin.framework/` are present into `ppmplugin/<name>.ppmplugin`, and verifies the archive layout with `jar tf` (exactly the manifest + the shipped binaries — nothing missing, nothing extra). Output: a statically verified `.ppmplugin` file on disk. Prereqs: `jar` (JDK). Run after /generate-ppmplugin-manifest and the build skill(s).

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Compile a PAM control's iOS Obj-C/Swift module into the FLAT device-slice `.framework` for a `.ppmplugin` bundle (never an `.xcframework` — the wrap CI won't descend into one). Mac-only (Xcode). Builds from a throwaway staged copy so canonical `ios/` and the podspec stay untouched, references React-Core headers only (React is weak-linked and provided by the wrap host at runtime, no CocoaPods), generates the required umbrella header, module map and Info.plist, sets the critical build settings, asserts manifest conformance, then runs a device-only `xcodebuild archive` and copies out the flat framework to `ppmplugin/staging/ios/`. Known limitation — the React weak-link flags aren't yet validated against a live wrap host and the RN pin must match the host's. Run after /generate-ppmplugin-manifest for an iOS or Both target, before /assemble-ppmplugin.

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Designing in-product tours, tooltips, and contextual help that teach product capabilities without becoming friction. Trigger logic, tour architecture, contextual placement, completion tracking. Honest about tooltip-spam (visual noise that users develop blindness to), one-and-done (help invisible at the moment of need), and contextual-when-needed (surfaces help at the moment friction occurs) patterns. Triggers on product tour, in-product tooltip, contextual help, walkthrough, feature tour, hint system, in-app guidance, tour platform. Also triggers when feature adoption is low, when users miss key product capabilities, or when an in-product help system is being scoped for the first time.

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Content distribution as a discipline. Owned channels (newsletter, blog, social), earned channels (PR, syndication, mentions), paid channels (boosted posts, syndication networks), and the channel-fit decisions that distinguish strategic distribution from spam-everywhere. Audience-channel matching, content-channel matching, distribution cadence. Triggers on content distribution, channel strategy for content, owned earned paid channels, content amplification, content promotion, audience-channel matching, content-channel matching, distribution cadence, syndication strategy, organic distribution. Also triggers when content is publishing but reach is low, when the team is distributing on every channel without strategy, or when the content program needs a distribution discipline rather than just publication.

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Build email template systems with React Email or MJML: responsive layouts, dark mode, multi-provider sending, and spam score optimization. Use when setting up transactional email, building email design systems, or debugging deliverability.

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Send an email campaign via a connected ESP with spam and compliance checks, approval-gated. "send this newsletter to the active list"

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Provides R wrappers of several on-target and off-target scoring methods for CRISPR guide RNAs (gRNAs). The following nucleases are supported: SpCas9, AsCas12a, enAsCas12a, and RfxCas13d (CasRx). The available on-target cutting efficiency scoring methods are RuleSet1, Azimuth, DeepHF, DeepCpf1, enPAM+GB, and CRISPRscan. Both the CFD and MIT scoring methods are available for off-target specificity prediction. The package also provides a Lindel-derived score to predict the probability of a gRNA to

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Expert knowledge for Azure Virtual Network Manager development including troubleshooting, best practices, architecture & design patterns, limits & quotas, security, and configuration. Use when managing AVNM network groups/topologies, IPAM pools, connectivity hubs, UDRs, or security admin rules, and other Azure Virtual Network Manager related development tasks. Not for Azure Virtual Network (use azure-virtual-network), Azure Virtual WAN (use azure-virtual-wan), Azure Networking (use azure-networking), Azure Network Watcher (use azure-network-watcher).

日本語の概要は準備中です。原文の説明を表示しています。