「sequences」の検索結果

Build quiz and assessment funnels that generate qualified leads at 30-50% conversion. Use when the user mentions "quiz funnel", "scorecard", "lead magnet", "score-based segmentation", or "lead qualification". Also trigger when designing self-assessment tools, building calculators or graders for marketing, or creating personalized result pages that drive conversions. Covers concept hooks, question design, dynamic results by tier, and automated follow-up sequences. For landing page conversion, see cro-methodology. For full marketing plans, see one-page-marketing.

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Clarify brand messaging using narrative structure that positions the customer as hero. Use when the user mentions "brand message", "website copy", "elevator pitch", "one-liner", "brand script", "StoryBrand framework", "customer as hero", or "my messaging isnt resonating". Also trigger when rewriting homepage copy, crafting email nurture sequences, or creating consistent messaging across sales and marketing collateral. Covers landing page copy, marketing collateral, and consistent communication. For memorable messaging, see made-to-stick. For product positioning, see obviously-awesome.

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Build a scalable outbound B2B sales machine with specialized roles (SDR, AE, CSM). Use when the user mentions "outbound sales", "Cold Calling 2.0", "cold email sequences", "sales pipeline", "SDR process", "sales development", "build an outbound sales team", or "fill my pipeline". Also trigger when setting up a B2B SaaS sales team from scratch or building a lead-qualification framework to improve close rates. Covers the three lead types (seeds/nets/spears), role specialization, the referral-email method, ANUM qualification, and pipeline math. For offer design, see hundred-million-offers. For persuasion science, see influence-psychology.

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Apply named refactoring transformations to improve code structure without changing behavior. Use when the user mentions "refactor this", "code smells", "extract method", "replace conditional", "technical debt", "move method", "inline variable", "decompose conditional", or "clean up this messy code". Also trigger when cleaning up legacy code, preparing code for new features by restructuring, or identifying which transformation fits a specific code smell. Covers smell-driven refactoring, safe transformation sequences, and testing guards. For code-quality foundations, see clean-code. For managing complexity, see software-design-philosophy.

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Owns architecture, engineering delivery, infrastructure, data platform, and internal systems. Use this for build-versus-buy calls, technology selection, architectural direction, engineering capacity and delivery risk, technical debt tradeoffs, platform and tooling decisions, or when a technical choice has business consequences that need stating in business terms. Also use to judge whether a technical plan is sound before it is committed to.

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Finds, qualifies, and reaches prospects through cold outreach — list building, qualification criteria, cold email and multi-channel sequences, and the follow-up that actually gets replies. Use this to build a prospect list, write cold outreach, fix a sequence that is not getting responses, define qualification criteria, or decide whether a segment is worth pursuing.

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Runs voice and meeting infrastructure — phone systems and numbers, emergency calling obligations, conference rooms and their AV, call recording and its retention consequences, and the porting that makes provider changes go badly. Use this to replace a phone system, fix rooms nobody can start a meeting in, meet emergency-calling requirements, port numbers without losing service, or work out why voice quality degrades only sometimes.

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Designs automated email and SMS programs — welcome and onboarding sequences, nurture, re-engagement, transactional messaging, and the timing and segmentation behind them. Use this to build or fix an email sequence, plan lifecycle automation, improve open or click performance, set up SMS flows, or decide what messages a customer should receive and when.

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Systematically maps mechanism evidence for a disease from molecules to pathways, cell types, tissues, biological consequences, and clinical phenotypes. Always use this skill when a user needs a layered mechanism evidence chain rather than a flat summary or immediate gap analysis. Formal literature citations must be real and verifiable.

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Analyze images and multi-frame sequences using OpenAI GPT vision models

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Analyze images and multi-frame sequences using OpenAI GPT series

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Create Architecture Decision Records (ADRs) documenting significant technical choices with context, options, consequences, and sequential numbering.

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Create Mermaid diagrams for flowcharts, sequences, ERDs, and architectures. Masters syntax for all diagram types and styling. Use PROACTIVELY for visual documentation, system diagrams, or process flows.

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Build or restructure a course syllabus from a topic list or reading list — course description + prerequisites, week-by-week schedule (topic → readings → deliverables), measurable learning objectives, an assessment scheme + rubric, standard policies (late work, AI use, academic integrity, accessibility), and a per-week work-list to hand to `/create-lecture`. Use when user says "build a syllabus", "structure my course", "turn this reading list into a schedule", "draft a course outline", "make a syllabus for Econ 7xx", or "map weeks to lectures". Economics-aware (PhD metrics/micro/macro sequences, undergrad); generic enough for any field.

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Create an Architecture Decision Record (ADR) for any technical decision. Use when asked to document a technical decision, write an ADR, record an architecture choice, or capture why a technology or approach was selected. Produces a structured ADR with context, decision, consequences, and tradeoffs.

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Read and write Kit (ConvertKit): list subscribers, broadcasts, sequences, tags; draft broadcasts. Trigger phrases: kit, convertkit, newsletter, broadcasts, subscribers.

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End-to-end LinkedIn outreach campaign builder. Takes leads from Supabase, upstream skills, or CSV. Aligns on campaign goal and tone, writes personalized LinkedIn message sequences (connection request + follow-ups + optional InMail), presents for review, and exports for the user's outreach tool (Dripify, Botdog, Expandi, or manual CSV). Logs to Supabase outreach_log.

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Set up a complete outbound email campaign in Smartlead. Asks the user for campaign goal, audience, messaging, schedule, and mailbox allocation. Creates the campaign, adds leads, saves email sequences, sets schedule, and assigns available mailboxes. Use when a user wants to launch email outreach via Smartlead.

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Write and simplify C# property-based, model-based, and executable specification tests with CsCheck. Use for generators, shrinking, stateful command sequences, Spec guards and rules, implementation conformance, or replaying generated failures.

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Parses, queries, converts, and extracts from GTF and GFF3 gene-model annotation files - walking the gene/transcript/exon/CDS hierarchy with gffutils (queryable SQLite DB), converting formats and extracting transcript/CDS/protein FASTA with gffread, slurping to dataframes with gtfparse/pyranges, and sanitizing malformed files with AGAT. Covers the 1-based-inclusive vs 0-based BED coordinate conversion (start-1 only), deriving implicit features (introns/UTRs/TSS), phase-not-frame, the stop-codon-in-or-out-of-CDS convention, and the chr1-vs-1 seqid and gene-ID-version mismatches that silently produce all-zero count matrices and dropped joins. Use when extracting features or sequences from an annotation, converting GTF<->GFF3 or GTF->BED, traversing the gene tree, or diagnosing a coordinate/provenance mismatch upstream of counting or DE.

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Assigns pathogen lineages (SARS-CoV-2 Pangolin UShER mode; Nextclade clade + QC; pango-designation alias resolution) and tracks variant frequencies over time using Nextstrain (Augur + Auspice), wastewater deconvolution (Freyja, COJAC, alcov, lineagespot), lineage-fitness modelling (multinomial logistic), and recombinant detection (3SEQ, RDP4, Bolotie). Covers Pangolin pangolin-data and Nextclade dataset version pinning (mandatory; lineage-defining mutations change with dataset), Freyja barcode forward-only date constraint, ARTIC primer scheme churn (V3/V4/V4.1/V5.3.2/Midnight) with dropout regions, and recombinant X-prefix designation lag. Use when assigning Pango lineages and Nextclade clades to viral consensus sequences, building Nextstrain Augur surveillance pipelines, deconvolving wastewater into lineage frequencies with Freyja, tracking lineage frequencies over time, handling ARTIC primer dropouts, or running surveillance for SARS-CoV-2/influenza/Mpox/RSV/H5N1/measles.

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Calculates statistical power for high-dimensional genomics experiments (bulk RNA-seq, scRNA-seq, ATAC-seq, ChIP-seq, methylation, proteomics) under negative-binomial count models using RNASeqPower, PROPER, and simulation via powsimR, distinguishing per-gene from marginal (transcriptome-wide) power, the role of mean expression and dispersion, and the sequencing-depth-versus-replicate tradeoff. Covers simulation as the honest default for overdispersed counts, FDR-aware average power versus single-test power, observed/post-hoc power as an anti-pattern, and the winner's-curse / Type-S / Type-M consequences of underpowering. Use when planning replicate number for a sequencing experiment, deciding whether to add depth or samples, choosing closed-form versus simulation power, estimating power from pilot dispersions, or justifying replication in a grant. For clinical-trial power see clinical-biostatistics/power-and-sample-size; for the inverse sample-size question see experimental-design/sample-size.

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Run remote BLAST searches against NCBI servers using Biopython Bio.Blast.NCBIWWW. Use when identifying unknown sequences, finding homologs, picking the correct BLAST program (blastn/blastp/blastx/tblastn/tblastx/psiblast/megablast/dc-megablast), interpreting Karlin-Altschul E-values, avoiding the max_target_seqs trap (Shah 2019), choosing composition-based statistics, or limiting searches by organism. Covers RID lifecycle, database choice (nt/nr/refseq_select/swissprot), word-size and CBS taxonomy.

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Query UniProt's REST API (post-2022 endpoint at rest.uniprot.org) for protein sequences, annotations, GO terms, cross-references, ID mappings, and proteomes. Use when fetching UniProtKB entries, navigating the JSON schema, choosing between UniProtKB/UniRef/UniParc/Proteomes resources, deciding stream vs search endpoint for batch retrieval, running ID-mapping jobs with the async pattern, handling isoform suffixes, or filtering reviewed Swiss-Prot vs auto-annotated TrEMBL. Encodes the legacy URL migration (2022), the new JSON schema layout, and bulk-pull patterns.

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