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Designs and analyzes in vivo CRISPR screens in animal tumor models, organoids, and immune-cell adoptive transfers. Covers bottleneck math (250x cells/sgRNA requires ~25M cells implanted; impossible for most syngeneic models, forcing focused libraries), focused library design (Manguso 2017 Nature 547:413 immune screen; Chen 2015 tumor screens), CRISPR-StAR intrinsic-control screening (Uijttewaal 2025 Nat Biotechnol 43:1848), clonal-dynamics-limited detection, tumor-explant DNA recovery, syngeneic vs xenograft vs PDX considerations, and the relationship to downstream MAGeCK / drugZ analysis. Use when designing in vivo CRISPR screens for tumor / immune / metastasis biology, choosing focused vs genome-wide for animal models, addressing bottleneck-induced clonal collapse, picking the syngeneic / xenograft / PDX model, integrating in vivo with in vitro results, or applying CRISPR-StAR for animal experiments.

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Agent fallback for CCSPlayerPawn_SprayPaint (auto-generated, category: func). Locate CCSPlayerPawn::SprayPaint in the CS2 server module via IDA Pro MCP and emit a fresh, minimal-unique artifact. The deterministic preprocessor could not resolve this symbol on the current gamever - your job is the re-sign. Trigger: CCSPlayerPawn_SprayPaint, CCSPlayerPawn::SprayPaint

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Java Spring Bootサービスにおける認証/認可、バリデーション、CSRF、シークレット、ヘッダー、レート制限、依存関係セキュリティのSpring Securityベストプラクティス。

Spring Bootアーキテクチャパターン、REST API設計、レイヤードサービス、データアクセス、キャッシング、非同期処理、ログ。Java Spring Bootバックエンド作業に使用。

Comprehensive spreadsheet creation, editing, and analysis with support for formulas, formatting, data analysis, and visualization. When Claude needs to work with spreadsheets (.xlsx, .xlsm, .csv, .tsv, etc) for: (1) Creating new spreadsheets with formulas and formatting, (2) Reading or analyzing data, (3) Modify existing spreadsheets while preserving formulas, (4) Data analysis and visualization in spreadsheets, or (5) Recalculating formulas

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Use this skill any time a spreadsheet file is the primary input or output. This means any task where the user wants to: open, read, edit, or fix an existing .xlsx, .xlsm, .csv, or .tsv file (e.g., adding columns, computing formulas, formatting, charting, cleaning messy data); create a new spreadsheet from scratch or from other data sources; or convert between tabular file formats. Trigger especially when the user references a spreadsheet file by name or path — even casually (like "the xlsx in my downloads") — and wants something done to it or produced from it. Also trigger for cleaning or restructuring messy tabular data files (malformed rows, misplaced headers, junk data) into proper spreadsheets. The deliverable must be a spreadsheet file. Do NOT trigger when the primary deliverable is a Word document, HTML report, standalone Python script, database pipeline, or Google Sheets API integration, even if tabular data is involved.

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Use this skill any time a spreadsheet file is the primary input or output. This means any task where the user wants to: open, read, edit, or fix an existing .xlsx, .xlsm, .csv, or .tsv file (e.g., adding columns, computing formulas, formatting, charting, cleaning messy data); create a new spreadsheet from scratch or from other data sources; or convert between tabular file formats. Trigger especially when the user references a spreadsheet file by name or path — even casually (like "the xlsx in my downloads") — and wants something done to it or produced from it. Also trigger for cleaning or restructuring messy tabular data files (malformed rows, misplaced headers, junk data) into proper spreadsheets. The deliverable must be a spreadsheet file. Do NOT trigger when the primary deliverable is a Word document, HTML report, standalone Python script, database pipeline, or Google Sheets API integration, even if tabular data is involved.

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Create, read, edit, fix, or clean spreadsheet files (.xlsx, .xlsm, .csv, .tsv). Use whenever a build task's artifact kind is spreadsheet, or the task names a spreadsheet file and wants something done to it or produced from it, including restructuring messy tabular data into a proper workbook. Covers openpyxl generation, formulas and recalculation, editing existing workbooks, and the validation gates. Not for tasks whose deliverable is a document, report, or web page that merely contains a table.

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Prepare a defense-contractor environment for CMMC Level 2 certification: scope CUI and FCI, implement the 110 NIST SP 800-171 Rev 2 security requirements across 14 families, compute the SPRS score with the DoD Assessment Methodology, manage a compliant POA&M, and ready the organization for a C3PAO assessment. Use when an organization handles Controlled Unclassified Information (CUI) under a DoD contract, when a contract carries DFARS clause 252.204-7012/7019/7020/7021, when preparing for or responding to a CMMC assessment, when computing or improving an SPRS score, when building a System Security Plan or POA&M for 800-171, or when scoping which systems are in the CUI boundary. Keywords: CMMC, CMMC Level 2, NIST 800-171, SP 800-171 Rev 2, CUI, FCI, SPRS, DFARS 7012, C3PAO, POA&M, System Security Plan, DoD Assessment Methodology, 110 controls, defense industrial base, DIB, FedRAMP equivalency.

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Advanced Scrum Master skill for data-driven agile team analysis and coaching. Use when the user asks about sprint planning, velocity tracking, retrospectives, standup facilitation, backlog grooming, story points, burndown charts, blocker resolution, or agile team health. Runs Python scripts to analyse sprint JSON exports from Jira or similar tools: velocity_analyzer.py for Monte Carlo sprint forecasting, sprint_health_scorer.py for multi-dimension health scoring, and retrospective_analyzer.py for action-item and theme tracking. Produces confidence-interval forecasts, health grade reports, and improvement-velocity trends for high-performing Scrum teams.

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Plan a sprint with capacity estimation, story selection, dependency mapping, and risk identification. Use when preparing for sprint planning, estimating team capacity, selecting stories, or balancing sprint scope against velocity.

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Explicit-only last resort. Draws game art from code, with no image model - pixel sprites from a PixelSpec, flat vector props, UI and icons from portable SVG, rigged characters with planted feet, FX with a JS runtime, seam-proven autotiles, playable top-down layouts with collision, stylized parallax and ambient plate motion - palette-exact, with QA and review sheets. Use only when the user explicitly asks for code-drawn art, or when generate2dmedia route_media.py finds no image or video route (no-route, exit 3). Never the default - image generation (generate2dsprite, generate2dmap, video2dsprite) comes first for characters, props, maps and FX. Not for painterly, detailed or likeness art, HD-2D plates or realistic fire, smoke or water. Always disclose the result as code-drawn.

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The art-route layer of the Forge skills. route_media.py is the one command that generates an image or an image-to-video clip - through the paid API of any provider whose key is configured (OpenAI, Google Gemini, xAI, BytePlus ModelArk, fal.ai; the configured key is the owner's consent), else through the user's own signed-in Codex or Grok CLI - and prints one JSON line with the route, artifact, sha256 and cost estimate; with no route it answers no-route (codeart2d is then the last resort). Also the capability check (forge_doctor), API key configuration, the spend ledger, resume and Codex image adoption. Use when a Forge skill sends a generation here, for the capability check, or when the user asks about keys, routes or spend. Not for planning, processing or QA of sprites, video frames or maps (generate2dsprite, video2dsprite, generate2dmap) or for code-drawn art (codeart2d).

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[omh] Team minutes, retros, and decision history to keep: meeting minutes, scrum/sprint records, retros, decisions, and follow-up history. Use when the user says: operating-rhythm, operating rhythm, meeting minutes, meeting history, scrum record, sprint planning, sprint review, sprint retrospective.

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Generates images and game sprites through GPT or Grok with guided provider choices, separate saved defaults, automatic cleanup and optional curation. Handles sprite requests, ordinary image generation/editing, standalone image-to-video clips (i2v, animate this still, 그록 영상, 이매진 비디오, 스틸 움직여줘, first/last frame, reference-to-video, 영상 이어붙이기, 영상 편집, extend/edit a clip), clips from a video MCP on the agent (ZCRE, 지크 MCP), chroma removal, animation atlases, video loops, 큐레이션뷰, image candidates, 팔레트 스왑, palette swap, recolor, rig layers, engine exports, repeating backgrounds, projected shadows, motion/contact inspection and optional scene composition from existing assets.

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Scrum-inspired paper review, revision, and R&R workflow. Handles docx/tex/md/PDF in English or Chinese. Auto-detects manuscript stage, estimates sprint count, runs multi-lens review (Contribution/Rigor/Writing/Editor), generates prioritized revision backlog, exports MD/DOCX/PDF/HTML reports. Use when asked to review a paper, revise based on reviewer comments, handle R&R, respond to peer review, plan paper revision sprints, or when user types /ps or /papersprint.

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Für Bonitätsprüfung: ordnet Norm, Beweislast und Gegenargument; Ergebnis: Tatbestands- oder Anspruchsmatrix. Fachgebiet: AGB-Recht-Prüfer. Route: bonitaetspruefung.

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Für Bonitätsprüfung: ordnet Norm, Beweislast und Gegenargument; Ergebnis: Tatbestands- oder Anspruchsmatrix. Fachgebiet: AGB-Recht-Prüfer. Route: agb-bonitaetspruefung-score-klauseln.

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Analyzes single-cell pooled CRISPR screens (Perturb-seq, CROP-seq, Perturb-CITE-seq, ECCITE-seq, multiome) where each cell carries an sgRNA and a scRNA-seq / surface-protein / chromatin readout. Covers experimental design (direct-capture Perturb-seq Dixit 2016 vs CROP-seq 3'UTR-barcoded Datlinger 2017 vs ECCITE-seq vs Multiome), MOI for sgRNA assignment, escaper-cell filtering (Mixscape, Papalexi 2021), SCEPTRE NB GLM + permutation for low-MOI (Barry 2024 Genome Biol 25:124), the Pertpy framework, factor decomposition, genome-scale Perturb-seq (Replogle 2022 Cell, 2.5M cells), and per-perturbation single-cell DE. Use when running a single-cell CRISPR screen, choosing direct-capture vs CROP-seq architecture, filtering escaper cells, performing single-cell DE, integrating Perturb-seq with pathway analysis, scaling to GW CRISPRi via Replogle protocol, or analyzing multi-omics screens.

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Analyzes pooled CRISPR screens with MAGeCK (Li et al 2014), covering count generation (mageck count), the RRA two-condition workflow (mageck test using alpha-RRA over per-sgRNA negative-binomial p-values), the MLE multi-condition workflow (mageck mle with explicit design matrix and beta-score output), normalization choice (median vs total vs control-sgRNA vs spike-in), sgRNA efficiency injection, paired-sample testing, time-course design, drug-screen versus dropout-screen design matrices, MAGeCKFlute and MAGeCK-VISPR downstream visualization, and decision logic for when to use MAGeCK vs JACKS / BAGEL2 / drugZ / Chronos. Use when running a fresh CRISPR screen analysis, picking RRA vs MLE for the experimental design, choosing a normalization method from QC signatures, debugging MLE convergence failure or NaN beta scores, comparing MAGeCK output across tools, or building a batch-aware multi-cell-line / multi-condition MLE design matrix.

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Generates Spring Boot 3.x configurations, creates REST controllers, implements Spring Security 6, sets up Spring Data JPA, and configures reactive WebFlux endpoints.

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Expert patterns for 2D animation in Godot using AnimatedSprite2D and skeletal cutout rigs. Use when implementing sprite frame animations, procedural animation (squash/stretch), cutout bone hierarchies, or frame-perfect timing systems. Trigger keywords: AnimatedSprite2D, SpriteFrames, animation_finished, animation_looped, frame_changed, frame_progress, set_frame_and_progress, cutout animation, skeletal 2D, Bone2D, procedural animation, animation state machine, advance(0).

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Provides functionalities to visualize and contextualize CRISPR guide RNAs (gRNAs) on genomic tracks across nucleases and applications. Works in conjunction with the crisprBase and crisprDesign Bioconductor packages. Plots are produced using the Gviz framework.

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Apply core coding standards, dependency injection, and configuration for Spring Boot 3. Use for Spring Boot application structure and DI/configuration; defer Spring Security hardening and test-specific setup to their focused skills.

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