「library」の検索結果

Normalizes ChIP-seq data using exogenous spike-in (ChIP-Rx with Drosophila chromatin per Orlando 2014 / Egan 2016; E. coli carryover for CUT&RUN/CUT&Tag). Distinguishes RRPM from Rx-Input scaling, integrates with DiffBind / DESeq2 / edgeR / csaw via sizeFactors and DiffBind library-size vectors, and applies the Patel et al 2024 *Nat Biotechnol* review's failure-mode framework to validate that normalization is correctly applied at the read level (not peak counts). Use when global signal shifts are expected (HDACi, BETi, EZH2i, dosage, target knockdown), when ChIPseqSpikeInFree detects post-hoc shifts, or when validating internal-control regions before publication.

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Analyzes alternative splicing at single-cell resolution. The first decision is library chemistry — 10X 3' is fundamentally limited (RT primes from poly-A, R2 falls in 3' UTR, <0.1 junction read per cell per AS event). Plate-based full-length methods (Smart-seq3, FLASH-seq, VASA-seq, STORM-seq) and single-cell long-read (MAS-Iso-seq, scISOr-Seq2) are the chemistries that give per-cell isoform structure. Tools include MARVEL (R, Smart-seq integrated), BRIE2 (Bayesian PSI with regulatory features and ELBO_gain test), scQuint (junction-cluster, plate-based; not for 10X), SpliZ (annotation-free Z-score), Psix (graph-smoothness regulated AS), and Sierra (alternative polyadenylation, often confused with AS). Use when analyzing isoform usage in scRNA-seq, identifying cell-type-specific splicing, or determining whether scRNA-seq chemistry supports splicing analysis at all.

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Identify differentially accessible chromatin regions across conditions using DiffBind, csaw, DESeq2, or edgeR. Use when comparing ATAC-seq accessibility between treatment groups, choosing between consensus-peak vs sliding-window approaches, picking the correct normalization (full library vs reads-in-peaks), correcting batch with SVA/RUVseq, or interpreting log2FC and FDR thresholds in a chromatin context.

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Extract and consolidate reusable components, design tokens, and patterns into your design system. Identifies opportunities for systematic reuse and enriches your component library. Use when the user asks to create components, refactor repeated UI patterns, build a design system, or extract tokens.

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Azure Identity SDK for .NET. Authentication library for Azure SDK clients using Microsoft Entra ID. Use for DefaultAzureCredential, managed identity, service principals, and developer credentials.

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Azure Key Vault Keys SDK for .NET. Client library for managing cryptographic keys in Azure Key Vault and Managed HSM. Use for key creation, rotation, encryption, decryption, signing, and verification.

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Azure Event Grid SDK for .NET. Client library for publishing and consuming events with Azure Event Grid. Use for event-driven architectures, pub/sub messaging, CloudEvents, and EventGridEvents.

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Azure OpenAI SDK for .NET. Client library for Azure OpenAI and OpenAI services. Use for chat completions, embeddings, image generation, audio transcription, and assistants.

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Advanced JavaScript animation library skill for creating complex, high-performance web animations.

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Forces the laziest solution that actually works, simplest, shortest, most minimal. Channels a senior dev who has been paged at 3am for a factory class that wrapped one function: question whether the task needs to exist at all, reach for the standard library before custom code, native platform features before dependencies, one line before fifty. Supports intensity levels: lite, full, ultra. The default is full. Use on ANY coding task: writing, adding, refactoring, fixing, reviewing, or designing code, and choosing libraries or dependencies. Also use whenever the user says "ponytail", "be lazy", "lazy mode", "simplest solution", "minimal solution", "yagni", "do less", or "shortest path", or complains about over-engineering, bloat, boilerplate, or unnecessary dependencies. Do NOT use for non-coding requests such as general knowledge, prose, translation, summaries, recipes.

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Develop Openrind Shell's OpenShell-supervised PostgreSQL FUSE runtime, compatibility sync runtime, and just-bash library.

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Authoritative resource for building, auditing, and improving GNOME GTK4/libadwaita apps. Design tokens, 42-widget component library, 21 pattern skills, 47 mirrored HIG pages, 34 real-app audits, and lint scripts. Use when building a GNOME app, adding GNOME GUI features, auditing HIG compliance, packaging for Flatpak/Flathub, or when the user mentions GTK, libadwaita, Adw, Blueprint, or GNOME HIG.

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Search Haskell APIs using Hoogle. Use when working with Haskell projects to look up function signatures, find functions by type, discover library documentation, or identify which module exports an item.

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Render Mermaid diagrams as SVG and PNG using the Beautiful Mermaid library. Use when the user asks to render a Mermaid diagram.

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Build beautiful, accessible UI components using shadcn/ui library with Radix UI primitives and Tailwind CSS styling that you own and can customize. Use when creating accessible component libraries, implementing common UI patterns (dialogs, dropdowns, tooltips), building forms with validation, creating data tables, implementing command palettes, using Radix UI primitives, customizing components with Tailwind, or building design systems with full ownership of code.

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Create well-structured, comprehensive Claude skills with clear activation criteria, detailed descriptions, usage examples, and proper documentation following best practices. Use when documenting new workflows, creating reusable skill patterns, establishing coding standards as skills, building team knowledge base, or expanding the skills library with new capabilities.

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When the user wants to create, plan, or optimize a lead magnet for email capture or lead generation. Also use when the user mentions "lead magnet," "gated content," "content upgrade," "downloadable," "ebook," "cheat sheet," "checklist," "template download," "opt-in," "freebie," "PDF download," "resource library," "content offer," "email capture content," "Notion template," "spreadsheet template," or "what should I give away for emails." Use this for planning what to create and how to distribute it. For interactive tools as lead magnets, see free-tool-strategy. For writing the actual content, see copywriting. For the email sequence after capture, see email-sequence.

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Forces the laziest solution that actually works, simplest, shortest, most minimal. Channels a senior dev who has seen everything: question whether the task needs to exist at all (YAGNI), reach for the standard library before custom code, native platform features before dependencies, one line before fifty. Supports intensity levels: lite, full (default), ultra. Use on ANY coding task: writing, adding, refactoring, fixing, reviewing, or designing code, and choosing libraries or dependencies. Also use whenever the user says "ponytail", "be lazy", "lazy mode", "simplest solution", "minimal solution", "yagni", "do less", or "shortest path", or complains about over-engineering, bloat, boilerplate, or unnecessary dependencies. Do NOT use for non-coding requests (general knowledge, prose, translation, summaries, recipes).

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Code review focused exclusively on over-engineering. Finds what to delete: reinvented standard library, unneeded dependencies, speculative abstractions, dead flexibility. One line per finding: location, what to cut, what replaces it. Use when the user says "review for over-engineering", "what can we delete", "is this over-engineered", "simplify review", or invokes /ponytail-review. Complements correctness-focused review, this one only hunts complexity.

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Create a new command file following repo conventions. Use when you need to add a new slash command, create automation workflows, or extend the command library with new functionality.

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Idiomatic Go - package and interface design, error wrapping, table-driven tests, generics, the modern standard library (slices/maps/cmp/errors.Join), current syntax, and logging discipline. Use when writing, reviewing, or refactoring any Go code, especially code drifting toward Java/Spring shapes (deep layer trees, generic repositories, heavy frameworks) - even if the user never says "idiomatic". Distilled from spf13/go-skills and adapted to this repo.

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Find the right skill for a capability. Load when a user or skill needs to check if a skill exists for a given task, when process-decomposer assigns skills to steps, or when agent-builder checks skill availability. Triggers on "what skill does this need", "find a skill for", "is there a skill that", "which skill handles", "does a skill exist for", "skill lookup", "check skill library". Prevents skill sprawl by always checking existing skills before creating new ones. The gatekeeper for all skill creation.

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Ground framework-specific implementation in official documentation — detect stack versions, fetch authoritative docs, implement cited patterns, cite sources. Load when building with a library or framework where API correctness matters, when the user wants verified or documented code, or before writing framework code from memory. Also triggers on "source driven development", "cite the docs", "check official documentation", "verify against docs", "don't hallucinate APIs". Not for pure logic or renames. Pairs with feature-spec and test-driven-development.

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Build Motion (Framer Motion) React animations — declarative motion components, variants, AnimatePresence, layout transitions, scroll effects, and inline SVG pathLength. Load when the user asks to animate with Motion, Framer Motion, motion.div, AnimatePresence, layout animation, whileInView, or React enter/exit transitions. Also triggers on "framer-motion", "motion/react", "useReducedMotion", or "React animation library". React/Next only — not README embeds or non-React.

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