2,259 件 ・ 関連度順
概要と使いどころ
Creates a Beads issue for a requested piece of work, completes exactly that work, and closes the issue. Use when the user invokes `/bx` or `$bx`, or asks for a bead to be created and the described work done in the same go. For creating a bead without doing the work, use the `bc` skill instead.
日本語の概要は準備中です。原文の説明を表示しています。
Define la identidad visual de un producto — propone dos o tres direcciones de color y tipografía justificadas por lo que el producto es, las escribe en design/tokens.css y las deja listas para verlas aplicadas en preview.html antes de decidir. Úsalo tras definir el producto y ANTES de prototipar (p. ej. "define la identidad", "qué paleta usamos", "elige los colores del producto"). No construye vistas.
日本語の概要は準備中です。原文の説明を表示しています。
Test-to-requirement coverage mapping. Reads plan acceptance criteria and test files, produces a coverage gap report showing which requirements have no tests. Use during Phase 2 (Test) or Phase 4 (Review) to verify test completeness. Named after the Nyquist sampling theorem — sufficient test coverage prevents aliased (missed) requirements. NOT for running tests (see mk:testing); NOT for structural code review (see mk:review).
日本語の概要は準備中です。原文の説明を表示しています。
Collect and submit developer feedback to the LiveAvatar team. Use when: (1) A LiveAvatar integration is done, working, finished, or complete, (2) The user says "it works", "we're done", "that's it", "ship it", (3) The user is frustrated, stuck, or giving up — "this is broken", "this doesn't work", "I'm stuck", "I give up", "this is frustrating", "I want to report an issue", (4) The user explicitly asks to give feedback, report a bug, file an issue, or share a suggestion about LiveAvatar, (5) You've seen 3+ failed attempts at the same integration step, (6) You just finished writing or generating LiveAvatar integration code for the user.
日本語の概要は準備中です。原文の説明を表示しています。
Build visual territories (mood boards) for team alignment before production. Groups references into 2-4 named territories, each with palette, texture, typography feel, and 4-6 generated sample tiles, so a team can agree on a direction. Use when the user says "moodboard", "visual territories", "align the team on a look", "reference board", or needs stakeholder sign-off on aesthetic before generating final assets. Part of the Creative Production set.
日本語の概要は準備中です。原文の説明を表示しています。
Find UX pains and opportunities fast through competitor scans, pattern research, and web search. Surfaces how others solve the same flow, common failure modes, and conventions users already expect. Use when the user says "research this UX", "how do others do X", "competitor scan", "find UX patterns", "what's the convention for Y", or before ideating a flow. Part of the Product Design set. Uses web search/fetch tools.
日本語の概要は準備中です。原文の説明を表示しています。
Pick a visual direction before generating any creative. Produces 3-6 named, distinct visual directions (e.g. premium minimal, bold maximalist, lifestyle/editorial, retro, techno-brutalist) with rationale + sample prompts, then generates one reference image per direction so the user can choose. Use when the user says "explore visual direction", "what style should this be", "give me directions/looks", or before ads/scene/shot work when no style is locked. Part of the Creative Production set.
日本語の概要は準備中です。原文の説明を表示しています。
Check and install prerequisites for the Todo app project. Use when setting up the development environment, when a user first opens the workspace, when troubleshooting missing tools, or when the user mentions setup, install, prerequisites, or environment.
日本語の概要は準備中です。原文の説明を表示しています。
Turn friction you just hit into a concrete change to the workflow, so the same problem doesn't recur.
日本語の概要は準備中です。原文の説明を表示しています。
Up-to-date Zig programming language patterns for version 0.17.0-dev (0.16.0 stable APIs plus the 0.17 deltas). Use when writing, reviewing, or debugging Zig code, working with build.zig and build.zig.zon files, or using comptime metaprogramming. Critical for avoiding outdated patterns from training data - especially std.net→std.Io.net (requires Io instance), std.time timestamps removed (use clock_gettime), std.Thread.Mutex/Condition/sleep removed (use pthreads), std.crypto.random removed, build system APIs (root_module, Compile methods→Module methods), I/O APIs (buffered writer pattern), container initialization (.empty/.init), allocator selection (DebugAllocator), ArrayList now unmanaged by default, @typeInfo lowercase fields (.@"struct" not .Struct), and removed language features (async/await, usingnamespace). Also covers 0.17.0-dev deltas: b.args→run_cmd.addPassthruArgs(), std.gpu→std.spirv, @bitCast logical-bit (endian-agnostic) semantics, build configurer/maker split, and the zig-pkg/ package directory. Also covers quality tooling Zig does not ship: coverage with kcov (and why in-file tests wreck the denominator), detecting unused private functions (neither the compiler nor zlint's unused-decls finds them), duplicate detection with jscpd, zlint (NOT the same-named X.509 linter in Homebrew), and the built-in fuzzer whose testOne now takes *std.testing.Smith rather than []const u8.
日本語の概要は準備中です。原文の説明を表示しています。
Capstone/orchestration skill — build an M-Agent + N-Human cooperative IDE in Rust gpui (the Harbor): many agents and humans co-editing the same files as co-equal CRDT replicas, governed by claims/guard/salvage, across LAN/shared/remote harbors. The INDEX that dispatches into the sibling rust skills. Use when building the collaborative editor, the agent-fleet console, multiplayer editing with agents-as-peers, or any slice of the Harbor. Trigger on: cooperative IDE, collaborative editor, multiplayer editor, agents and humans co-editing, gpui IDE, Loro CRDT editor, harbor editor, claims/salvage, "build the cooperative IDE". NOT for: a single non-collaborative gpui screen (compose the siblings directly), web editors, or non-editor apps.
日本語の概要は準備中です。原文の説明を表示しています。
Use when building content-heavy sites with Astro, deciding between SSG/SSR/hybrid, choosing client directives (idle/visible/load/media), structuring content collections with type-safe schemas, integrating React/Vue/Solid/Svelte components in the same project, or migrating from Next.js for marketing/docs. Triggers: client:idle vs client:load tradeoffs, content collections schema with zod, image optimization via @astrojs/image, view transitions, server islands, MDX layouts. NOT for SPA-style apps that need full interactivity (use Next/Remix), Astro internals/plugin authoring, or non-content sites where islands provide no benefit.
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Aligns and QCs methylated-RNA-immunoprecipitation (MeRIP / m6A-seq) IP and input libraries using STAR or HISAT2 splice-aware mapping, samtools sort/index, IP/input matched-pair tracking, antibody-lot metadata recording, replicate concordance via deepTools multiBamSummary + plotCorrelation, IP enrichment QC via plotFingerprint and per-transcript IP/input ratio distributions, library-complexity saturation curves via PreSeq, and the explicit do-NOT-deduplicate convention for standard non-UMI MeRIP. Use when preparing paired IP and input BAM files for exomePeak2 / MeTPeak / MACS3 peak calling, evaluating MeRIP replicate concordance and IP enrichment, deciding whether to deduplicate (standard MeRIP typically NOT), choosing genome-vs-transcriptome alignment for downstream peak vs m6Anet workflows, recording antibody clone and lot metadata for cross-batch reconciliation, detecting failed IPs via saturation curves and IP/input distribution shape, or generating IP-over-Input bigWig tracks for visualisation.
日本語の概要は準備中です。原文の説明を表示しています。
Bead-based signal normalization and cross-batch harmonization for CyTOF and high-parameter cytometry - EQ four-element bead normalization of instrument sensitivity drift (CATALYST normCytof, premessa), and reference-anchor cross-batch normalization (CytoNorm, per-cluster quantile splines). Covers the distinction between within-run drift correction and between-batch correction, the mandatory anchor/reference sample, why normalization is per-cluster with many quantiles, and the over-correction risk. Use when correcting CyTOF signal drift, harmonizing multi-batch or multi-site studies, or deciding whether to normalize data versus model batch in the design.
日本語の概要は準備中です。原文の説明を表示しています。
Quality control for flow, spectral, and mass cytometry - time-based anomaly cleaning (flowAI, flowCut, PeacoQC, flowClean), margin/boundary event removal, signal-drift detection, dead-cell exclusion, CyTOF Gaussian/DNA/event-length checks, instrument calibration/standardization (MESF, CS&T, peak-2), and batch-level outlier flagging. Use when assessing acquisition quality, choosing a cleaning tool, ordering QC relative to compensation, deciding margin removal before density-based steps, or flagging problematic samples before clustering or differential analysis.
日本語の概要は準備中です。原文の説明を表示しています。
Structures biological experiments so inference is valid by construction, covering Fisher's principles (randomization, replication, local control), the experimental-vs-observational unit distinction and pseudoreplication (Hurlbert 1984; Lazic 2018), randomization mechanics (complete, restricted, stratified, rerandomization, run-order), blocking layouts (randomized complete block, Latin square, incomplete block), factorial designs and interactions, and the split-plot/nested error strata hidden inside multi-batch genomics. Use when deciding the experimental unit and what counts as a replicate, planning randomization and run order, choosing a blocked/factorial/split-plot/nested layout, avoiding pseudoreplication in cell-culture or animal studies, or specifying the random-effects structure of the analysis model. For assigning samples to sequencing batches/lanes/plates and batch-effect correction see experimental-design/batch-design; for regulated clinical-trial randomization see clinical-biostatistics.
日本語の概要は準備中です。原文の説明を表示しています。
Differential abundance (DA) and differential state (DS) analysis for flow and mass cytometry - tests which cell populations change in frequency or marker expression between conditions using diffcyt (edgeR/voom/GLMM for DA, limma/LMM for DS), with cydar, CITRUS, and compositional methods (sccomp, scCODA, DCATS) as alternatives. Covers the sample-is-the-experimental-unit principle, design/contrast and mixed-model formulas, compositionality of cluster proportions, and FDR across clusters. Use when comparing populations between groups, choosing a DA method, handling paired/batch designs, or deciding whether compositional correction is needed.
日本語の概要は準備中です。原文の説明を表示しています。
Defines cell populations in flow and spectral cytometry through manual gates (rectangle, polygon, quadrant, boolean) and reproducible automated gating (openCyto gating templates, flowDensity data-driven thresholds, flowClust model-based gates), organized as a hierarchical GatingSet (flowWorkspace) and round-tripped with FlowJo via CytoML. Covers the canonical gate order (time -> debris -> singlets -> live -> lineage), FMO-vs-isotype boundary setting, gate-order dependence and recompute semantics, rare-event/MRD gating, and per-population statistics. Use when building a gating strategy, automating a manual FlowJo scheme across samples, choosing manual vs data-driven gates, or extracting population frequencies.
日本語の概要は準備中です。原文の説明を表示しています。
Performs alchemical free-energy calculations including relative binding free energy (RBFE / FEP+) and absolute binding free energy (ABFE) via OpenFE, FEP+, GROMACS, AMBER pmemd, and OpenMM with explicit lambda scheduling, soft-core potentials, MBAR/BAR analysis, cycle-closure validation, and protocol-appropriate enhanced sampling. Compares ML alternatives (Boltz-2 affinity, DeepDock). Use when ranking analogs by binding affinity beyond docking accuracy, performing prospective lead optimization, or validating SAR predictions.
日本語の概要は準備中です。原文の説明を表示しています。
Imports gene expression count matrices from featureCounts, HTSeq, STAR ReadsPerGene, Salmon/kallisto via tximport or tximeta, RSEM, 10X Genomics MTX/H5, AnnData H5AD, and RDS. Handles silent-miscounting traps (featureCounts -p v2.0.2 API break, STAR strandedness column choice, salmon NumReads-sum without tximport, RSEM non-integer expected_count, GENCODE _PAR_Y suffix, zero-length-transcript TPM divide-by-zero), and encodes the tximport countsFromAbundance decision tree with the "lengthScaledTPM is not TPM" warning. Use when assembling a gene-by-sample count matrix from aligner or quantifier output, importing salmon/kallisto for DESeq2 vs limma-voom, choosing strandedness column for STAR, debugging zero-count panics, or building tx2gene mapping.
日本語の概要は準備中です。原文の説明を表示しています。
Plot per-gene mutation distributions on a protein-domain map (lollipop / needle plots) showing mutation position, recurrence count, and variant classification with maftools, g3-lollipop, trackViewer, and ProteinPaint. Use when visualizing recurrent mutation hotspots on a single gene's protein, marking domain boundaries from UniProt/Pfam, comparing missense vs truncating distributions, or contrasting two cohorts on the same lollipop.
日本語の概要は準備中です。原文の説明を表示しています。
Plot per-group distributions of continuous data using boxplots, violins, beeswarms, quasirandom jitter, and raincloud plots with sample-size honesty (Weissgerber 2015), KDE-bandwidth awareness, and N-aware encoding choices. Use when comparing distributions across a small number of groups — expression per cluster, biomarker per arm, scores per condition — and the bar-of-mean default is misleading.
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Analyzes time-series and longitudinal RNA-seq for differential expression and trajectory structure. Covers DESeq2 LRT with reduced models, time as factor vs continuous vs natural splines, maSigPro (Nueda 2014 for RNA-seq), ImpulseDE2 with explicit impulse-model failure modes, DREAM for repeated measures via linear mixed models, pseudoreplication avoidance, conditional vs marginal modeling, and trajectory clustering with DPGP, Mfuzz (with Schwämmle 2010 fuzzifier estimation), and splines+k-means. Use when modeling time-course or longitudinal expression, choosing factor vs spline, handling repeated measures from the same subject, avoiding pseudoreplication, clustering temporal trajectories, or selecting between dedicated time-course tools and pairwise+LRT.
日本語の概要は準備中です。原文の説明を表示しています。
Mark and remove PCR/optical duplicates using samtools fixmate and markdup. Use when preparing alignments for variant calling or when duplicate reads would bias analysis.
日本語の概要は準備中です。原文の説明を表示しています。