4,205 件 ・ 関連度順
概要と使いどころ
Extract, process, and deduplicate reads using Unique Molecular Identifiers (UMIs) with umi_tools. Use when library prep includes UMIs and accurate molecule counting is needed, such as in single-cell RNA-seq, low-input RNA-seq, or targeted sequencing to distinguish PCR from biological duplicates.
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Preprocess ribosome profiling data including adapter trimming, size selection, rRNA removal, and alignment. Use when preparing Ribo-seq reads for downstream analysis of translation.
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Reads, writes, and converts molecular file formats (SMILES, SDF, MOL2, PDB) using RDKit and Open Babel. Handles structure parsing, canonicalization, and full standardization pipeline including sanitization, normalization, and tautomer canonicalization. Use when loading chemical libraries, converting formats, or preparing molecules for analysis.
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Visualize Hi-C contact matrices, TADs, loops, and genomic features using matplotlib, cooltools, and HiCExplorer. Create triangle plots, virtual 4C, and multi-track figures. Use when visualizing contact matrices or genomic features.
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Bisulfite sequencing read alignment using Bismark with bowtie2/hisat2. Handles genome preparation and produces BAM files with methylation information. Use when aligning WGBS, RRBS, or other bisulfite-converted sequencing reads to a reference genome.
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Species abundance estimation using Bracken with Kraken2 output. Redistributes reads from higher taxonomic levels to species for more accurate estimates. Use when accurate species-level abundances are needed from Kraken2 classification output.
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Deep learning-based variant calling from long reads using Clair3 for SNPs and small indels. Use when calling germline variants from ONT or PacBio alignments, particularly when high accuracy is needed for clinical or research applications.
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Calls DNA methylation from Oxford Nanopore sequencing data using signal-level analysis. Use when detecting 5mC or 6mA modifications directly from nanopore reads without bisulfite conversion.
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ChIP-seq quality control metrics including FRiP (Fraction of Reads in Peaks), cross-correlation analysis (NSC/RSC), library complexity, and IDR (Irreproducibility Discovery Rate) for replicate concordance. Use to assess experiment quality before downstream analysis. Use when assessing ChIP-seq data quality metrics.
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Predicts ADMET properties using ADMETlab 3.0 API or DeepChem models. Estimates bioavailability, CYP inhibition, hERG liability, and 119 toxicity endpoints with uncertainty quantification. Filters for PAINS and other structural alerts. Use when filtering compounds for drug-likeness or prioritizing leads by predicted safety.
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Options trading strategy analysis and simulation tool. Provides theoretical pricing using Black-Scholes model, Greeks calculation, strategy P/L simulation, and risk management guidance. Use when user requests options strategy analysis, covered calls, protective puts, spreads, iron condors, earnings plays, or options risk management. Includes volatility analysis, position sizing, and earnings-based strategy recommendations. Educational focus with practical trade simulation.
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Ingest a raw source document into the wiki. Reads the source, extracts key information, creates or updates wiki pages, maintains cross-references, and logs the operation. Use when the user adds a new source or says 'ingest this'.
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Use when the user asks to "turn this idea into posts for every platform", "write the X thread / LinkedIn post / 小红书 note", or "spec the carousel slides"; turns one idea into N platform-native ready-to-paste packages — post/thread, caption, Threads text, 小红书 note, link post with first-comment placement — each adapted per the dated platform norm card (never verbatim cross-posting), every product claim held to approved claims-ledger wording or flagged [needs source], every variant tagged {formula | hook family | CTA type | signal optimized}, plus a carousel slide-spec mode (intro/content/outro slide roles, per-element char budgets, 小红书 3:4 / IG 4:5 / LinkedIn document PDF artboards, text-safe-zone + alt-text checklist). Not for creator deliverable briefs — use brief-generator; not for repurposing existing assets with paid boost — use content-amplifier. 社媒文案/一稿多发改写/小红书笔记/轮播图脚本
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Use when the user asks to "plan the participation ramp before we promote", "how much account history or karma do we need in this community", or "design entry incentives and member lifecycle for our own Discord"; produces the per-community pre-promotion warming plan — account-history/tenure expectations (Estimated, named sources), a give-before-ask ledger spec, a per-community etiquette + rule digest with last-verified dates, and the warming → active graduation criteria that channel-registry requires as state-transition evidence — plus the owned-community variant (entry paths + member lifecycle for your own Discord/Slack/forum/企业微信私域). Not for launch-day submissions or T-0 threads — use community-launch-runner. 社区预热/先给后取/账号养成/毕业标准
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Use when the user asks to "codify how our founder sounds", "build a founder voice dossier", or "set our content pillars"; runs an 80%-extraction interview over the user's OWN posts, emails, and decks (never competitor scraping, never an invented persona) and produces the versioned voice record — a per-platform register map (incl. 小红书/微信公众号), banned phrases, per-context disclosure lines (the ECHO C2 upstream), a few-shot bank built exclusively from own posts, and 3-5 content pillars with Estimated %-allocations — submitted via memory/events/channels.ndjson for channel-registry to store as voice-dossier.md, the record every Craft-phase skill reads first. Not for the brand-level voice canon — use brand-language-codifier; not for audience/persona research — use audience-mapper. 声音档案/品牌语气/创始人语气/内容支柱/披露声明
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Use when the user asks to "set up my founder social-selling routine", "build a daily engagement block for target accounts", or "turn funding / hiring signals into selling plays"; produces the founder/seller daily operating block — a time-boxed engagement-block spec (substantive value-add comments on target-account posts, never a pitch), warm-touch-before-ask cadence rules, trigger-response plays consuming the social-pulse-monitor B2B trigger watchlist (funding / hiring / launch signals), and a quarterly diagnostic that reads LinkedIn SSI as an input diagnostic only (Estimated, vendor-defined — never a target metric). All volume norms are Estimated/community-derived; all execution is human — zero mass-DM, zero connection-request automation, zero engagement automation (LinkedIn UA §8.2 red line); 1:1 pitch and DM mechanics route to outreach-manager. Not for cold email sequences — use cold-outbound-sequencer. 社交销售/创始人日常互动块/热身触达节奏/触发信号剧本
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Use when the user asks to "triage launch feedback", "cluster reviews, comments, and board posts into themes", or "set up a you asked, we shipped loop"; produces a feedback theme digest (frequency, severity, representative quotes per theme), an open→planned→started→completed/declined status loop with duplicate-merge and notification rules, shipped-change announcement material, and a compliant social-proof harvest protocol (never incentivized store reviews). Not for repurposing or amplifying the harvested proof — use content-amplifier; not for executing testimonial outreach threads — use outreach-manager. 反馈分诊/状态环/社证收割/评测合规
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Use when the user asks to "map our positioning", "name our competitive alternatives", or "pick a beachhead segment for the launch"; produces a Dunford-style positioning canvas — named competitive alternatives (including spreadsheet and status quo), unique attributes (verifiable, or routed to the claims ledger), value themes (attribute→benefit→value chains), a target beachhead segment scored on serviceability / pain intensity / reachability, and a one-sentence onlyness statement — the sole upstream of the message house and the entity-signal source for the canonical entity profile. Not for the message house or per-channel launch copy — use message-house-builder; not for audience/persona profiling itself — use audience-mapper; not for SEO keyword positioning — use keyword-research. 定位画布/竞争替代品/独特价值/滩头细分
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Use when the user asks to "monitor my launch", "track our Product Hunt / Hacker News ranking", or "watch the launch window"; runs the T-0 to T+30 window watch — pre-launch instrumentation verification (UTM/event checks, the upstream of RAMP P1), HN rank/points/comments polling with a comments-over-points flamewar early-warning (Estimated heuristic), PH votes/featured status, store charts and reviews, news echo, D0/W1/M1 KPI snapshots vs targets, spike-vs-sustain and owned-capture reads, and alert thresholds against the launch-tier KPI targets. Not for launch-day go/rollback calls — use launch-day-conductor; not for metric deep-dives — use performance-analyzer; not for SEO rank tracking — use rank-tracker. 发布监控/排名轮询/火焰战比/spike-sustain
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Use when the user asks to "read back" a paid campaign change, "did this ad change work", or "compare ROAS/CPA before and after"; reads ROAS/CPA against a control over a fixed readback window and returns a Promote / Keep-testing / Rollback / Unproven readback decision with the math delegated to roi-calculator. Not for RQS scoring or veto adjudication — use ad-account-auditor; not for the ROI ratio math — use roi-calculator; not for cross-channel rollups — use performance-analyzer. 付费广告复盘/ROAS回看/投放效果归因
日本語の概要は準備中です。原文の説明を表示しています。
Use when the user asks to "analyze influencer campaign performance", "compare influencers", or "find what content worked"; produces metric scorecards vs target and benchmark, platform/influencer/content rankings, engagement-quality and sentiment reads, conversion-attribution breakdowns, and ranked learnings. Not for dollar-level return math — use roi-calculator. 达人营销效果分析/投放复盘
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Use when platform-reported conversions disagree with GA4/ecommerce, when you suspect Meta and Google are double-counting the same sales, or for a standing (monthly) reconciliation workbook that de-dups stacked credit against an order-ID truth set, normalizes attribution windows and currency, compares attribution models, and reads incrementality from a geo/holdout test. Not for the point-in-time R2 veto or RQS gate — use ad-account-auditor; not for the ROI/ROAS ratio math itself — use roi-calculator; not for organic dark-social share attribution or GA4 direct-traffic decomposition — use dark-social-attributor. 付费广告归因对账/去重/增量
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Use when the user asks to "is my ad fatiguing", "why is CTR dropping at scale", or "should I rotate creative / widen the audience"; reads frequency, CTR and CVR decay against an early-flight baseline and returns Rotate-creative / Widen-audience / Hold triggers with a per-ad-set fatigue read. Not for building the replacement creative — use ad-creative-builder; not for the RQS score or vetoes — use ad-account-auditor. 广告疲劳检测/频次管理/换素材还是扩人群
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Researches the app being cloned and reads what its real users say in public reviews (App Store, Google Play, G2, Capterra, Trustpilot, Reddit, Hacker News, its own feature-request board), then ranks what they hate, what is missing and what is unsolved, turns it into a fix plan for the clone and a positioning angle you can sell. Every quote is real, verbatim and linked. Use when the user says "what do people hate about X", "read the reviews", "how do I make mine better", "find the gap", "what features are missing", "how do I position this", "make it sellable", or after /replica-diff.
日本語の概要は準備中です。原文の説明を表示しています。