「cluster」の検索結果

Plan, create, and configure production-ready Azure Kubernetes Service (AKS) clusters. Covers Day-0 checklist, SKU selection (Automatic vs Standard), networking options (private API server, Azure CNI Overlay, egress configuration), security, and operations (autoscaling, upgrade strategy, cost analysis). WHEN: create AKS environment, provision AKS, enable AKS observability, design AKS networking, choose AKS SKU, secure AKS, optimize AKS, AKS spot nodes, AKS cluster-autoscaler, rightsize AKS pod, pod rightsizing, over-provisioned AKS pod, pod resource requests and limits, Vertical Pod Autoscaler, VPA recommendations.

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Use when deciding WHERE to point SEO effort, not how to write a page. Triggers: a new site or brand with no rankings and no authority ("cold start", "starting from zero", "nobody knows us"), deciding what to double down on, a page or cluster that ranks but earns nothing, hunting emerging or newly-coined keywords before competitors arrive, "should we build a cluster or one page", "what do we write next", "we get impressions but no clicks", "our traffic plateaued despite publishing", whether to chase a head term at all, or turning any of it into recurring automation. Also use when asked why an SEO effort stalled despite consistent output. NOT for writing an article, keyword expansion mechanics, or auditing a single tactic for penalty risk.

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Patterns for building entity actors with Akka.Hosting - GenericChildPerEntityParent, message extractors, cluster sharding abstraction, akka-reminders, and ITimeProvider. Supports both local testing and clustered production modes.

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Akka.Management for cluster bootstrapping, service discovery (Kubernetes, Azure, Config), health checks, and dynamic cluster formation without static seed nodes.

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Assign cell types from marker expression in IMC/MIBI data using clustering (PhenoGraph/FlowSOM/Leiden/Pixie), marker-based probabilistic classifiers (Astir), or image-context CNNs (CellSighter), covering the double-positive segmentation artifact, lineage-vs-state markers, the two spillover types, and why a "cell type" in imaging is conditioned on a segmentation guess. Use when phenotyping segmented IMC cells, choosing clustering vs classification, diagnosing implausible double-positive populations, separating lineage from functional markers, or transferring labels across a cohort.

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Infer or annotate TCR antigen specificity by unsupervised clustering (TCRdist/tcrdist3, GLIPH2, clusTCR, GIANA) and database lookup (VDJdb, IEDB, McPAS-TCR), and rank candidates with supervised predictors (ERGO-II, NetTCR-2.x, pMTnet) under explicit caveats. Encodes the central truth that general TCR-epitope prediction for UNSEEN epitopes essentially does not work (collapses to near-random; IMMREP22, Grazioli 2022) because labeled data is dominated by a few immunodominant epitopes and there is no true negative set — so clustering for discovery is the honest task and de-novo binding needs wet-lab validation. Use when annotating TCR specificity or grouping a repertoire. Epitope/MHC context lives in mhc-binding-prediction.

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Bead-based signal normalization and cross-batch harmonization for CyTOF and high-parameter cytometry - EQ four-element bead normalization of instrument sensitivity drift (CATALYST normCytof, premessa), and reference-anchor cross-batch normalization (CytoNorm, per-cluster quantile splines). Covers the distinction between within-run drift correction and between-batch correction, the mandatory anchor/reference sample, why normalization is per-cluster with many quantiles, and the over-correction risk. Use when correcting CyTOF signal drift, harmonizing multi-batch or multi-site studies, or deciding whether to normalize data versus model batch in the design.

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Differential abundance (DA) and differential state (DS) analysis for flow and mass cytometry - tests which cell populations change in frequency or marker expression between conditions using diffcyt (edgeR/voom/GLMM for DA, limma/LMM for DS), with cydar, CITRUS, and compositional methods (sccomp, scCODA, DCATS) as alternatives. Covers the sample-is-the-experimental-unit principle, design/contrast and mixed-model formulas, compositionality of cluster proportions, and FDR across clusters. Use when comparing populations between groups, choosing a DA method, handling paired/batch designs, or deciding whether compositional correction is needed.

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Detects and removes doublets/aggregates from flow, spectral, and mass cytometry before clustering or quantification. Covers FSC-A vs FSC-H singlet discrimination (the Area-Height non-proportionality, not a 1D area gate), FSC-W/SSC width gating, CyTOF Gaussian discrimination parameters (Center/Offset/Width/Residual/Event_length) and DNA intercalator gating, and the residual heterotypic conjugates that survive scatter gating and masquerade as double-positive populations. Use when filtering aggregates before phenotyping, choosing a doublet method for flow vs CyTOF, or diagnosing a suspicious double-positive cluster.

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Analyzes time-series and longitudinal RNA-seq for differential expression and trajectory structure. Covers DESeq2 LRT with reduced models, time as factor vs continuous vs natural splines, maSigPro (Nueda 2014 for RNA-seq), ImpulseDE2 with explicit impulse-model failure modes, DREAM for repeated measures via linear mixed models, pseudoreplication avoidance, conditional vs marginal modeling, and trajectory clustering with DPGP, Mfuzz (with Schwämmle 2010 fuzzifier estimation), and splines+k-means. Use when modeling time-course or longitudinal expression, choosing factor vs spline, handling repeated measures from the same subject, avoiding pseudoreplication, clustering temporal trajectories, or selecting between dedicated time-course tools and pairwise+LRT.

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Process and analyze single-cell ATAC-seq data with Signac, ArchR, SnapATAC2, or Cell Ranger ATAC. Use when handling 10X scATAC or 10X Multiome (paired RNA+ATAC) data, performing per-cell QC, choosing between ArchR/Signac/SnapATAC2 ecosystems, building per-cluster consensus peaksets, integrating with paired scRNA-seq, doublet detection (AMULET vs ArchR vs scDblFinder), or running pseudobulk differential accessibility per cluster.

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Manages MongoDB Atlas Stream Processing (ASP) workflows. Handles workspace provisioning, data source/sink connections, processor lifecycle operations, debugging diagnostics, and tier sizing. Supports Kafka, Atlas clusters, S3, HTTPS, and Lambda integrations for streaming data workloads and event processing. NOT for general MongoDB queries or Atlas cluster management. Requires MongoDB MCP Server with Atlas API credentials.

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AWS EKS Kubernetes management for clusters, node groups, and workloads. Use when creating clusters, configuring IRSA, managing node groups, deploying applications, or integrating with AWS services.

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Deploy, scale, and manage Kubernetes workloads. Create deployments, services, and configurations, manage cluster resources, troubleshoot pods, and implement production-ready Kubernetes patterns. Use when working with Kubernetes clusters, K8s deployments, or container orchestration.

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When the user wants to research keywords, find target keywords, or analyze search intent. Also use when the user mentions "keyword research," "keyword tool," "target keywords," "search volume," "search intent," "keyword difficulty," "topical map," "keyword clustering," "People Also Ask," "Google autocomplete," "autocomplete keywords," or "alphabet method." For clusters, use content-strategy.

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Kubernetes diagnostics for metrics, health checks, resource comparisons, and cluster analysis. Use when analyzing cluster health, comparing environments, or gathering diagnostic data.

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Manage kind (Kubernetes IN Docker) local clusters. Use when creating, testing, or developing with local Kubernetes clusters in Docker containers.

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Develop a content strategy covering editorial positioning, content pillars, formats, calendar, governance, and topical authority planning. Use this skill whenever the user wants to plan a content program, define content pillars, build an editorial calendar, structure topic clusters, set up content governance, or align content production with broader brand and SEO goals. Triggers on content strategy, content plan, editorial strategy, content pillars, content calendar, editorial calendar, topical authority, topic clusters, content governance, content roadmap, content production. Also triggers when the user is about to start producing content without a strategic plan.

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Remote command execution and file transfer on SageMaker HyperPod cluster nodes via AWS Systems Manager (SSM). This is the primary interface for accessing HyperPod nodes — direct SSH is not available. Use when any skill, workflow, or user request needs to execute commands on cluster nodes, upload files to nodes, read/download files from nodes, run diagnostics, install packages, or perform any operation requiring shell access to HyperPod instances. Other HyperPod skills depend on this skill for all node-level operations.

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Diagnose and remediate cluster-wide HyperPod (EKS or Slurm) problems — creation / deployment failures (CloudFormation, EFA health check, lifecycle scripts, capacity), EKS access, node replacement, CloudFormation nested-stack errors, post-maintenance rollback state, dangling nodes, autoscaler conflicts. Includes `--validate` pre-flight. Read-only.

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Plan content strategy: content pillars, audience research, topic clustering, funnel mapping, and content audits. Use when deciding what content to create, building a content calendar, planning topic clusters, or finding content gaps.

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SGC is a semi-supervised pipeline for gene clustering in gene co-expression networks. SGC consists of multiple novel steps that enable the computation of highly enriched modules in an unsupervised manner. But unlike all existing frameworks, it further incorporates a novel step that leverages Gene Ontology information in a semi-supervised clustering method that further improves the quality of the computed modules.

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treekoR is a novel framework that aims to utilise the hierarchical nature of single cell cytometry data to find robust and interpretable associations between cell subsets and patient clinical end points. These associations are aimed to recapitulate the nested proportions prevalent in workflows inovlving manual gating, which are often overlooked in workflows using automatic clustering to identify cell populations. We developed treekoR to: Derive a hierarchical tree structure of cell clusters; qua

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syntenet can be used to infer synteny networks from whole-genome protein sequences and analyze them. Anchor pairs are detected with the MCScanX algorithm, which was ported to this package with the Rcpp framework for R and C++ integration. Anchor pairs from synteny analyses are treated as an undirected unweighted graph (i.e., a synteny network), and users can perform: i. network clustering; ii. phylogenomic profiling (by identifying which species contain which clusters) and; iii. microsynteny-bas

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