「condition」の検索結果

Identifies differential m6A methylation between conditions from MeRIP-seq paired IP/input data using exomePeak2 with `bam_ip` + `bam_input` (control arm) and `bam_treated_ip` + `bam_treated_input` (treatment arm) for integrated GC-bias-aware differential calling (Liu 2022 *NAR Genom Bioinform* 4:lqac046), QNB beta-binomial test (Liu 2017 *BMC Bioinformatics* 18:387), MeTDiff HMM-based differential bundled with MeTPeak, RADAR (Zhang 2019 *Genome Biol* 20:294) with its `filterBins -> diffIP -> reportResult` workflow, and the defensible paired-symmetric case of edgeR / DESeq2 on featureCounts-on-peaks matrices when batch / lot covariates need explicit fixed-effect handling (exomePeak2's top-level API does NOT accept arbitrary covariates). Covers paired vs unpaired vs interaction designs, batch confounding (antibody lot, RNA prep, sequencing run) and the per-lot meta-analysis strategy when exomePeak2 is the primary caller, the stoichiometry-vs-expression-vs-IP-efficiency confound that all MeRIP differential methods inherit, normalisation choice (size factor on IP, on input, on per-sample IP/input ratio), the McIntyre 2020 reproducibility caveat, effect-size filtering as a guardrail against under-powered N=2 designs, and orthogonal-validation routes for absolute stoichiometry (GLORI / SAC-seq / m6Anet `mod_ratio`). Use when comparing m6A levels across two or more conditions, choosing between exomePeak2 / QNB / RADAR / MeTDiff for a given design, handling batch confounding when exomePeak2's API is too rigid, normalising against input properly, distinguishing real hyper- / hypo-methylation from expression-level shifts, applying effect-size thresholds, interpreting volcano plots of differential peaks, or planning a follow-up orthogonal stoichiometry validation.

日本語の概要は準備中です。原文の説明を表示しています。

Compare gene co-expression and regulatory networks between biological conditions to find rewired relationships using DiffCorr, DiffCoEx, DINGO/iDINGO, and CoDiNA. Covers the differential-connectivity-is-not-differential-expression distinction, the pairwise multiple-testing explosion, marginal vs partial (direct) rewiring, and the underpowered-rewiring failure mode. Use when comparing co-expression networks between disease vs control, treatment, or developmental stages, or finding hub genes that rewire without changing mean expression. For single-condition modules see coexpression-networks; for differential expression of means see differential-expression/de-results.

日本語の概要は準備中です。原文の説明を表示しています。

Detects m6A modifications from Oxford Nanopore direct-RNA-sequencing (ONT DRS) signal data using m6Anet (Hendra 2022 *Nat Methods* 19:1590; multiple-instance-learning neural network over DRACH 5-mer signal). Covers the required upstream pipeline (Dorado / Guppy basecalling -> minimap2 transcriptome alignment with `-ax map-ont -uf -k14 --secondary=no` -> nanopolish eventalign with `--scale-events --signal-index` (m6Anet-required) plus `--summary` / `--threads` housekeeping -> m6anet dataprep -> m6anet inference), per-site vs per-read probability interpretation including the `mod_ratio` per-site stoichiometry column, the DRACH-only modeling constraint, minimum-coverage thresholds (20-50 reads per site for stable probability estimates), multi-condition comparison via xPore (Pratanwanich 2021 *Nat Biotechnol* 39:1394), Nanocompore (Leger 2021 *Nat Commun* 12:7198), ELIGOS (Jenjaroenpun 2021 *NAR* 49:e7), and Dorado native modification calling (RNA004 chemistry, 2024+), reference-transcriptome version pinning, the cDNA-vs-DRS chemistry distinction (cDNA-Nanopore CANNOT be used for modification detection), and orthogonal validation against MeRIP / GLORI. Use when calling m6A from ONT DRS without immunoprecipitation, choosing m6Anet vs xPore vs Nanocompore vs ELIGOS vs Dorado native, interpreting per-site `probability_modified` vs `mod_ratio` vs per-read modification probabilities, comparing methylation between conditions from ONT data, deciding between m6Anet for known DRACH sites and Dorado/Remora for genome-wide screening, pinning RNA002 vs RNA004 chemistry and basecaller model versions, or troubleshooting eventalign / dataprep failures.

日本語の概要は準備中です。原文の説明を表示しています。

Batch effect correction for CRISPR screens covering ComBat empirical-Bayes, RUV, SVA, control-sgRNA normalization, and the model-based alternative of including batch as a covariate in MAGeCK MLE or Chronos. Covers screen-specific batch sources (passage cohort, library lot, infection day, sequencing run, Cas9 lot, FBS lot), PCA + variance-decomposition diagnostic to decide if correction is needed, when correction harms biology by over-correcting condition into batch, limma removeBatchEffect for visualization-only correction, and relationship to multi-condition design matrices. Use when combining screens for joint analysis, when passage cohort confounds biology, when DepMap-style panels need Chronos with batch covariates, when picking ComBat vs RUV, or when correction harms biology and should be replaced with explicit covariate modeling.

日本語の概要は準備中です。原文の説明を表示しています。

Identify differentially bound regions across CLIP-seq conditions (knockdown vs control, treatment vs vehicle, disease vs healthy) using DEWSeq (sliding-window DESeq2), Flipper (Skipper-downstream), ASpeak, edgeR, or limma-voom. Use when computing condition-level changes in RBP binding intensity, choosing peak-level vs window-level vs crosslink-level testing, designing replicate experiments, or distinguishing biological binding shifts from technical confounders.

日本語の概要は準備中です。原文の説明を表示しています。

Identifies super-enhancers from H3K27ac, MED1, or BRD4 ChIP-seq using ROSE, ROSE2, LILY, HOMER -style super, and ENCODE dELS cross-referencing. Handles peak stitching parameters, ranking choices, hockey-stick inflection, marker choice (H3K27ac vs MED1/BRD4), and cross-condition comparison with spike-in normalization. Constructs core regulatory circuitry (Saint-Andre 2016) from SE-encoded TFs. Use when identifying cell-identity / cancer-associated regulatory domains, comparing super-enhancers between conditions, identifying master transcription factor networks, or predicting BET-inhibitor responsiveness.

日本語の概要は準備中です。原文の説明を表示しています。

Identifies differentially bound ChIP-seq regions between conditions using DiffBind, csaw (sliding windows), DESeq2/edgeR/PyDESeq2 on count matrices, NormR (control-aware), or MAnorm2. Distinguishes three distinct normalization problems (composition bias, trended bias, global shifts) and matches each to its appropriate fix including spike-in scaling. Use when comparing ChIP-seq binding between experimental conditions, choosing normalization for global vs local changes, integrating spike-in data, or reconciling DiffBind/DESeq2 disagreement.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when a driver encounters adverse weather, road, or traffic conditions and needs to extend driving time. Covers eligibility criteria, the 2-hour maximum extension, documentation requirements, and what does NOT qualify.

日本語の概要は準備中です。原文の説明を表示しています。

Use this skill when the user asks about Americans with Disabilities Act (ADA) accommodation for CDL drivers — interaction between ADA + FMCSA medical certification, when DOT-disqualifying conditions trigger ADA analysis, reasonable accommodation case examples, employer obligations, and how to handle a driver with a condition that may affect CDL status. Cite Title I ADA + 49 CFR 391.41.

日本語の概要は準備中です。原文の説明を表示しています。

Systematic debugging methodology covering root cause analysis, log-based debugging, production debugging, memory leak diagnosis, deadlock detection, performance anomaly investigation, and reproducible bug reproduction. Distilled from real experience at Google, DeepMind, ByteDance, and Huawei. USE WHEN: investigating production incidents, debugging hard-to-reproduce bugs, analyzing crash dumps, finding race conditions, diagnosing memory leaks, debugging performance regressions, or any situation requiring systematic root cause analysis. Triggers on "debugging", "root cause", "bug", "crash", "segfault", "deadlock", "race condition", "memory leak".

日本語の概要は準備中です。原文の説明を表示しています。

Auditing Microsoft Entra ID (Azure Active Directory) configuration to identify risky authentication policies, overly permissive role assignments, stale accounts, conditional access gaps, and guest user risks using AzureAD PowerShell, Microsoft Graph API, and ScoutSuite.

日本語の概要は準備中です。原文の説明を表示しています。

You are helping a penetration tester exploit race conditions and TOCTOU

日本語の概要は準備中です。原文の説明を表示しています。

Time-of-Check / Time-of-Use (TOCTOU) race condition exploitation methodology across binary, kernel, filesystem, web, and container layers. Covers symbolic-link races (open/access/stat split), file-descriptor races, fopen/realpath traversal races, /proc and procfs races, FUSE-backed slow-fs races to widen the window, ptrace and signal races, kernel double-fetch / userspace pointer races, container/runc/symlink escape primitives, kubernetes admission/authz TOCTOU, web auth-vs-authz TOCTOU, JWT-claim TOCTOU at gateway vs service, payment/idempotency races, and modern race-amplification techniques (single-packet attack, slow loris, FUSE pause, cgroup freeze, scheduler shaping). Use when you've identified a 'check then act' pattern in code, when fuzzing for race conditions, or when exploiting concurrency bugs in privileged binaries / kernel / orchestrators.

日本語の概要は準備中です。原文の説明を表示しています。

OpenClawの機能分類とQAの証拠を照合し、品質・機能の充足度・長期サポートの評価を見直して、成熟度のスコアカード文書を更新するスキルです。

• 機能領域ごとの成熟度を見直したいとき
• 想定した利用手順の充足度を確認したいとき
• QAの証拠から評価文書を更新したいとき

XとLinkedInのつながりから、会いたい相手への紹介経路を評価するスキル。経路の近さや関係の強さを重み付けし、紹介を頼む候補と接点の不足を整理します。

• 対象者を紹介できる知人探し
• 共通の知人を紹介価値で順位付け
• 対象企業への紹介経路を調べたいとき

ウォレットや取引の実行権限を持つAIエージェントについて、外部入力への対策、支出上限、送信前の検証、停止条件、秘密鍵の管理を設計・点検するスキル。

• 取引権限を持つボットを監査したいとき
• 取引ごと・日ごとの支出上限の設計
• 送信前検証と自動停止の設計

開発の目的に合うECCのコマンド群を選び、実行順序と停止条件を案内します。現在のファイルを基に一覧も整理し、複数コマンドの使い分けを助けます。

• バグ修正のコマンド順序を知りたいとき
• MVP開発のコマンドを選びたいとき
• ECCの作業手順を一覧で見たいとき

C++のテスト作成・修正から、GoogleTestとCTestの設定、失敗原因の調査まで支援します。実行結果が不安定なテストの改善やカバレッジ測定にも対応します。

• C++の単体・結合テストを作りたいとき
• GoogleTestとCTestの設定
• 不安定なテストの原因調査

処理の高速化を、現状の計測、仮説ごとの変更案の比較、正しさの検証へ分解し、時間や費用の上限内で最も速い安全な案と再実行できる手順を残すスキル。

• 遅い処理を計測しながら高速化したいとき
• 同じ入力条件で実装案を比較したいとき
• 応答時間・処理量・実行費用の評価

AIエージェントが使うツールの種類や入出力、エラーからの復帰手順を設計・見直します。文脈の情報量も整理し、作業完了率や再試行回数で改善を評価します。

• エージェントのツールや入力形式の設計
• ツールの結果と次の行動を明確にしたいとき
• 安全な再試行と停止条件を定めたいとき

This skill should be used when the user asks to "create an agent", "add an agent", "write a subagent", "agent frontmatter", "when to use description", "agent examples", "agent tools", "agent colors", "autonomous agent", or needs guidance on agent structure, system prompts, triggering conditions, or agent development best practices for Claude Code plugins.

日本語の概要は準備中です。原文の説明を表示しています。

Build feature work with high overbuilding risk. Use for new behavior, product slices, or integrations where repository reuse, strict scope, and an explicit stop condition matter.

日本語の概要は準備中です。原文の説明を表示しています。

Prove existing work meets acceptance conditions without expanding scope. Use for validation-only tasks, completion checks, focused gate runs, and last-mile proof.

日本語の概要は準備中です。原文の説明を表示しています。

Create and execute Goal-Oriented Action Plans (GOAP) with precondition analysis, cost optimization, and adaptive replanning

日本語の概要は準備中です。原文の説明を表示しています。