Comprehensive truth scoring, code quality verification, and automatic rollback system with 0.95 accuracy threshold for ensuring high-quality agent outputs and codebase reliability.
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Comprehensive truth scoring, code quality verification, and automatic rollback system with 0.95 accuracy threshold for ensuring high-quality agent outputs and codebase reliability.
日本語の概要は準備中です。原文の説明を表示しています。
Write, review, filter, mock, and debug Core unit tests with the repository's installed Vitest 5 configuration. Use for tests under Core's Vitest include paths, `vi.mock`/spies/timers, jsdom component tests, coverage output, focused reruns, or unit-test failures. Do not use for browser E2E flows or async Next.js Server Components.
日本語の概要は準備中です。原文の説明を表示しています。
Master advanced prompt engineering techniques to maximize LLM performance, reliability, and controllability in production. Use when optimizing prompts, improving LLM outputs, or designing production prompt templates.
日本語の概要は準備中です。原文の説明を表示しています。
Complete guide for building accessible, high-UX forms in modern stacks (React/Next.js, Tailwind, Zod). Includes specific patterns for clickable areas, range sliders, output-inspired design, and WCAG compliance.
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Batch-resize or convert a folder of images for web, social media, slides, or ARCH-size print output while preserving originals. Use for image resizing, format presets, or photo batches; not for product-image background removal.
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GWAS and population genetics tool. Processes PLINK (.bed/.bim/.fam), VCF, and BGEN; runs QC (MAF, HWE, missingness), IBD estimation, PCA, and linear/logistic regression GWAS. Outputs Manhattan-ready summary stats. Use regenie or SAIGE for biobanks (>100k samples) needing mixed models.
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Splice-aware RNA-seq aligner producing sorted BAM and splice junction tables. Builds genome index, runs two-pass alignment for better junctions. Outputs sorted BAM, junctions (SJ.out.tab), stats (Log.final.out), optional gene counts. Use Salmon for fast pseudoalignment; STAR when a BAM is needed for variant calling, IGV, or ENCODE pipelines.
日本語の概要は準備中です。原文の説明を表示しています。
Guide to interpreting BUSCO completeness statuses: why Duplicated BUSCOs count as complete, parsing output files, computing/comparing completeness across proteomes/genomes, common counting mistakes. Use when running BUSCO QC, comparing assemblies, or reporting completeness. See also: prokka-genome-annotation for annotation workflows feeding BUSCO.
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Annotate prokaryotic genomes (bacteria, archaea, viruses) via Prokka's BLAST/HMM pipeline. Identifies CDS, rRNA, tRNA, tmRNA, signal peptides against Pfam, TIGRFAMs, RefSeq. Outputs GFF3, GenBank, FASTA, TSV. Use PGAP for NCBI GenBank submission; Bakta for faster NCBI-compatible annotation.
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Consensus cell type annotation: runs 10+ algorithms (KNN-Harmony/BBKNN/Scanorama/scVI, CellTypist, ONCLASS, Random Forest, SCANVI, SVM, XGBoost) on a labeled reference and transfers labels via majority voting. Outputs per-method labels, consensus, agreement score. Use when single-method annotation is insufficient or you need ensemble uncertainty for novel states.
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GSEA and over-representation analysis (ORA) for RNA-seq and proteomics. Wraps Enrichr for ORA against MSigDB, KEGG, GO, and 200+ databases; runs preranked GSEA on ranked DE gene lists. Outputs enrichment tables and running-score plots. Use after DESeq2 or edgeR for pathway-level interpretation.
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Automated scRNA-seq cell type annotation via pre-trained logistic regression. 45+ models: immune, gut, lung, brain, fetal, cancer microenvironments. Input normalized AnnData; outputs per-cell labels, majority-vote cluster labels, confidence scores. Use for fast, reference-backed annotation without manual marker inspection.
日本語の概要は準備中です。原文の説明を表示しています。
Ultra-fast RNA-seq transcript/gene quantification via quasi-mapping (no BAM). Builds a k-mer index from transcriptome FASTA, quantifies in minutes. Outputs TPM/count tables (quant.sf) with optional GC- and sequence-bias correction. Integrates with tximeta/tximport for DESeq2/edgeR. Use STAR when a genome-aligned BAM is needed.
日本語の概要は準備中です。原文の説明を表示しています。
Fast short-read DNA aligner for WGS/WES/ChIP-seq. 2× faster BWA-MEM successor; outputs SAM/BAM with read group headers for GATK. Primary plus supplementary records for chimeric reads. Use STAR for RNA-seq splice-aware alignment; Bowtie2 is a comparable alternative.
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AiZynthFinder retrosynthetic route planning (CASP) from AstraZeneca Molecular AI. Monte Carlo tree search guided by a template-based neural expansion policy recursively disconnects a target SMILES until precursors are found in a purchasable stock. Covers config.yml (v4 format), aizynthcli batch screening, the AiZynthFinder/AiZynthExpander Python API, one-step disconnections, custom stocks via smiles2stock, scorers, Retro*/breadth-first/DFPN search alternatives, and reading output.json.gz / trees.json. Use for synthesis route planning, synthesizability screening, and building-block/precursor search. For reaction barriers use neb-irc-activation-energy; for 2D reaction scheme drawing use rdkit-chemdraw-cdxml.
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Computational pathology toolkit for whole-slide images (WSIs): load slides, extract tiles, stain normalization, nuclear segmentation, feature extraction, and ML training. Supports H&E and multiplex. For end-to-end pipelines from raw WSIs to quantitative outputs.
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DL cell/nucleus segmentation for fluorescence and brightfield microscopy. Pre-trained models (cyto3, nuclei, tissuenet) and a generalist flow-based algorithm segment cells without retraining. Outputs label masks for morphology and tracking. Use scikit-image watershed for rule-based; Cellpose when DL generalization across staining is needed.
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Poisson-model peak caller for ChIP-seq/ATAC-seq BAMs. MACS3 callpeak finds enriched regions (TF sites or histone marks) vs input/IgG; outputs BED narrowPeak/broadPeak for motif analysis, annotation, and differential binding. Use narrow peaks for TF ChIP-seq and ATAC-seq; broad for H3K27me3, H3K9me3, and other broad marks.
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3Dmol.js WebGL molecular visualization emitted as self-contained HTML. Render structures (PDB/SDF/XYZ/MOL2/cube) with stick, sphere, cartoon, line, and surface styles; animate trajectories with a frame-delay (interval, ms) control; and animate vibrational normal modes via vibrate() from per-atom dx/dy/dz displacements or from precomputed frames. Output standalone HTML that loads 3Dmol from a CDN, with optional play/pause and speed controls. Use for transition-state imaginary-mode animations, MD or reaction-path playback, docking poses, and orbital/density isosurfaces. For static 2D chemical structure drawings use rdkit-chemdraw-cdxml; for 2D statistical plots use matplotlib or plotly.
日本語の概要は準備中です。原文の説明を表示しています。
Comprehensive truth scoring, code quality verification, and automatic rollback system with 0.95 accuracy threshold for ensuring high-quality agent outputs and codebase reliability.
日本語の概要は準備中です。原文の説明を表示しています。
Convert a markdown file to PDF using mistune + reportlab. Use when the user wants to convert a .md file to PDF, or when another skill needs to produce a PDF from markdown output.
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Consolidate IBRK trade CSV files from a directory into a summary report. Groups trades by symbol, underlying, date, strike, buy/sell, and open/close. Outputs both markdown and CSV.
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Analyze C++ build performance using vcperf.exe and ETL traces. Use this skill when the user wants to profile a build, understand compile bottlenecks, identify expensive headers or templates, generate JSON analysis reports, or interpret vcperf output. Covers tracing workflows, the /jsonAnalysis flag, reading the resulting JSON schema, and actionable remediation strategies for each bottleneck category.
日本語の概要は準備中です。原文の説明を表示しています。
SST v4 (Ion) expert for managing AWS resources as code with the Pulumi-backed framework. Use when writing or editing sst.config.ts, building infra/ modules (sst.aws.Function/Bucket/Dynamo/Cron/Service/Router, sst.Secret, sst.Linkable, raw aws.* Pulumi resources), wiring resource links, scoping IAM, or running sst deploy/dev/diff/remove. Essential when the user mentions SST, sst.config.ts, $config, $transform, $interpolate, sst.aws.*, sst.Secret, Pulumi/Ion, "sst deploy", a failed SST deploy (ConflictException on a resource-type change, "Identifier '__filename' has already been declared", MalformedPolicyDocument on an Output<T>), or wants to scaffold/troubleshoot AWS infrastructure with SST. Also use when a request to "deploy my AWS stack" or "add a Lambda/bucket/table" is made in a repo that already contains an sst.config.ts (using $config) or an sst dependency. Do NOT use when the task is primarily AWS CDK, Terraform, raw CloudFormation, or SAM with no SST present — those have their own tooling.
日本語の概要は準備中です。原文の説明を表示しています。