「planning」の検索結果

Answer Engine Optimization (AEO) skill — optimize content to be cited by AI language models (ChatGPT, Perplexity, Claude, Gemini, Mistral) as authoritative sources. Distinct from SEO — AEO optimizes for citation in LLM-generated responses, not search rankings. Use when planning content for AI-first search audiences, auditing existing content for E-E-A-T signals, tracking which pages get cited by which LLMs, or building a citation-friendly content strategy. Triggers — 'AEO audit', 'optimize for ChatGPT', 'get cited by Perplexity', 'LLM citation strategy', 'answer engine optimization', 'content for AI search', 'E-E-A-T audit'. Output is a markdown audit report (default) or JSON for pipeline integration. Stdlib-only Python tools.

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Technical research methodology with YAGNI/KISS/DRY principles. Phases: scope definition, information gathering, analysis, synthesis, recommendation. Capabilities: technology evaluation, architecture analysis, best practices research, trade-off assessment, solution design. Actions: research, analyze, evaluate, compare, recommend technical solutions. Keywords: research, technology evaluation, best practices, architecture analysis, trade-offs, scalability, security, maintainability, YAGNI, KISS, DRY, technical analysis, solution design, competitive analysis, feasibility study. Use when: researching technologies, evaluating architectures, analyzing best practices, comparing solutions, assessing technical trade-offs, planning scalable/secure systems.

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Identify the first beachhead market segment for a product launch. Evaluates segments against burning pain, willingness to pay, winnable market share, and referral potential. Use when choosing a first market, targeting an initial customer segment, or planning market entry strategy.

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Nominates and assesses CRISPR off-target sites genome-wide. Enumerates candidate sites by mismatch and bulge tolerance with Cas-OFFinder/CRISPRitz, ranks them with the published CFD score (SpCas9-only, relative ranker) or MIT/CRISTA/energy models, runs variant-aware screening against gnomAD/individual genomes (CRISPRme), and frames the empirical genome-wide discovery assays (GUIDE-seq, CIRCLE-seq, CHANGE-seq, DISCOVER-seq, Digenome-seq) and high-fidelity nuclease choice (HiFi Cas9, Sniper-Cas9, eSpCas9, SpCas9-HF1). Use when assessing guide RNA specificity, choosing among candidate guides, screening a therapeutic guide against population variation, or planning empirical off-target validation. Distinguishes predicted vs detected vs validated. On-target activity scoring and deaminase (Cas-independent) base/prime-editor off-targets are separate skills.

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Calculates statistical power for high-dimensional genomics experiments (bulk RNA-seq, scRNA-seq, ATAC-seq, ChIP-seq, methylation, proteomics) under negative-binomial count models using RNASeqPower, PROPER, and simulation via powsimR, distinguishing per-gene from marginal (transcriptome-wide) power, the role of mean expression and dispersion, and the sequencing-depth-versus-replicate tradeoff. Covers simulation as the honest default for overdispersed counts, FDR-aware average power versus single-test power, observed/post-hoc power as an anti-pattern, and the winner's-curse / Type-S / Type-M consequences of underpowering. Use when planning replicate number for a sequencing experiment, deciding whether to add depth or samples, choosing closed-form versus simulation power, estimating power from pilot dispersions, or justifying replication in a grant. For clinical-trial power see clinical-biostatistics/power-and-sample-size; for the inverse sample-size question see experimental-design/sample-size.

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Identifies differential m6A methylation between conditions from MeRIP-seq paired IP/input data using exomePeak2 with `bam_ip` + `bam_input` (control arm) and `bam_treated_ip` + `bam_treated_input` (treatment arm) for integrated GC-bias-aware differential calling (Liu 2022 *NAR Genom Bioinform* 4:lqac046), QNB beta-binomial test (Liu 2017 *BMC Bioinformatics* 18:387), MeTDiff HMM-based differential bundled with MeTPeak, RADAR (Zhang 2019 *Genome Biol* 20:294) with its `filterBins -> diffIP -> reportResult` workflow, and the defensible paired-symmetric case of edgeR / DESeq2 on featureCounts-on-peaks matrices when batch / lot covariates need explicit fixed-effect handling (exomePeak2's top-level API does NOT accept arbitrary covariates). Covers paired vs unpaired vs interaction designs, batch confounding (antibody lot, RNA prep, sequencing run) and the per-lot meta-analysis strategy when exomePeak2 is the primary caller, the stoichiometry-vs-expression-vs-IP-efficiency confound that all MeRIP differential methods inherit, normalisation choice (size factor on IP, on input, on per-sample IP/input ratio), the McIntyre 2020 reproducibility caveat, effect-size filtering as a guardrail against under-powered N=2 designs, and orthogonal-validation routes for absolute stoichiometry (GLORI / SAC-seq / m6Anet `mod_ratio`). Use when comparing m6A levels across two or more conditions, choosing between exomePeak2 / QNB / RADAR / MeTDiff for a given design, handling batch confounding when exomePeak2's API is too rigid, normalising against input properly, distinguishing real hyper- / hypo-methylation from expression-level shifts, applying effect-size thresholds, interpreting volcano plots of differential peaks, or planning a follow-up orthogonal stoichiometry validation.

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Structures biological experiments so inference is valid by construction, covering Fisher's principles (randomization, replication, local control), the experimental-vs-observational unit distinction and pseudoreplication (Hurlbert 1984; Lazic 2018), randomization mechanics (complete, restricted, stratified, rerandomization, run-order), blocking layouts (randomized complete block, Latin square, incomplete block), factorial designs and interactions, and the split-plot/nested error strata hidden inside multi-batch genomics. Use when deciding the experimental unit and what counts as a replicate, planning randomization and run order, choosing a blocked/factorial/split-plot/nested layout, avoiding pseudoreplication in cell-culture or animal studies, or specifying the random-effects structure of the analysis model. For assigning samples to sequencing batches/lanes/plates and batch-effect correction see experimental-design/batch-design; for regulated clinical-trial randomization see clinical-biostatistics.

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Estimates the minimum biological replicates (or cells/events) for a target power at a target FDR in genomics experiments using ssizeRNA, PROPER, powsimR for scRNA-seq, and pilot-data dispersion estimation from DESeq2/edgeR. Covers the biological-versus-technical replication distinction (technical replicates do not add degrees of freedom for biological inference), replicate-number-versus-sequencing-depth budgeting, scRNA-seq sample-versus-cell allocation under a pseudobulk model, and the critique that "n=3" is a publication convention rather than a power calculation. Use when budgeting a sequencing experiment, writing the sample-size justification in a grant, estimating replicates from pilot data, allocating a fixed budget between samples and depth, or planning scRNA-seq cohort size. For clinical-trial sample size see clinical-biostatistics/power-and-sample-size; for the power-given-n direction see experimental-design/power-analysis.

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Simulate transcription factor perturbation effects on cell state in silico with CellOracle and Dynamo, and predict transcriptional responses to genetic perturbations with GEARS, scGen, and CPA. Covers the direction-not-magnitude principle, local-linear validity, the GRN/velocity error it inherits, baseline discipline (mean and additive baselines), and the validation gap. Use when predicting TF knockout or overexpression effects, ranking driver TFs for fate transitions, or planning perturbation experiments. For GRN construction see multiomics-grn; for experimental Perturb-seq see single-cell/perturb-seq.

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Designs PROTACs, molecular glues, and bivalent degraders with explicit handling of E3 ligase choice (VHL, CRBN, IAP, MDM2, KEAP1), linker design (length, composition, rigidity), ternary complex prediction (PRosettaC, DeepTernary, AlphaFold3 with constraints), cooperativity (alpha), DC50 / Dmax characterization, hook effect, and prediction-experiment reconciliation. Use when designing targeted protein degraders, planning linker SAR, predicting ternary complex stability, or building generative degrader workflows.

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Designs adaptive clinical trials including group-sequential (O'Brien-Fleming, Pocock, Lan-DeMets spending), sample-size re-estimation (blinded Friede-Kieser, unblinded Cui-Hung-Wang, Mehta-Pocock promising zone), seamless Phase 2/3 with treatment-arm selection, population enrichment, and response-adaptive randomisation. Covers FDA 2019 Final Adaptive Designs Guidance, FDA 2022 Master Protocols, and ICH E20 Step 2b/3 draft (June 2025, NOT final). Use when planning interim analyses, sample-size re-estimation, or master/platform-trial designs.

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Analyzes chemical libraries by scaffold using Bemis-Murcko scaffolds, generic frameworks, cyclic skeletons, matched molecular pair (MMP) analysis via mmpdb, R-group decomposition, Free-Wilson analysis, scaffold hopping, and chemotype-aware ML train/test splits. Use when identifying chemotype clusters in a library, deriving SAR transformation rules, decomposing series into R-groups, performing scaffold-balanced QSAR splits, or planning analog campaigns.

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Build reactive, type-safe, production-grade backends. ALWAYS use this skill when the user asks to build, plan, design, or implement backend features, APIs, data models, server logic, database schemas, web apps, full stack apps, or mobile apps. This includes planning and architecture discussions.

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Use this skill when the user's prompt requires (1) researching a topic across multiple sources, comparing options or alternatives, analyzing trends or history, understanding markets or industries, or reviewing literature or studies and (2) synthesizing that research into a comprehensive, narrative report. If you're planning to search the web or internal knowledge bases, consider using this skill. This skill coordinates research subagents, so use it only when you have a tool for spawning subagents (the Agent or Task tool); otherwise, research the question directly.

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6 production-ready AI engineering workflows: prompt evaluation (8-dimension scoring), context budget planning, RAG pipeline design, agent security audit (65-point checklist), eval harness building, and product sense coaching.

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Expert in designing and building autonomous AI agents. Masters tool use, memory systems, planning strategies, and multi-agent orchestration.

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Skill for analyzing a project's existing UI in Vue. Activates when the user uses /pwdev-uiux:scan or asks to "analyze the project UI", "map existing components", "understand project patterns", or "create project skill". Generates .planning/ui/project-ui-skill.md with project-specific patterns and tokens.

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Use when a user asks for QA planning, execution, review, release assessment, reporting, status, or test-tool recommendations and the correct PWDEV QA workflow must be selected safely.

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Use when the user wants to set up pwdev-prd in a project — 'inicializar o pwdev-prd', 'init prd', 'configurar PRDs' — creating the .planning/prds/ workspace and choosing language and the optional SQLite audit trail. Do NOT use to write a PRD (prd-create).

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Use when the user wants a full feature action plan (backend + frontend + tests) with the PWDEVIA 7-question method before any code is written — 'planejar a feature X', 'criar plano de feature', 'plan the user CRUD'. Interviews the human (max 2 rounds) and writes .planning/feat/features/{slug}/plan.md. Do NOT use to write code (feat-exec), for small 1–3 file changes (feat-quick), backend-only or UI-only scope (feat-backend / feat-frontend), tests for existing code (feat-test) or code review (feat-review).

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Use when the user wants a UI-only action plan — components, pages, composables/hooks, state, forms and their E2E coverage — 'plano de frontend para a tela X', 'plan the settings page'. Writes .planning/feat/features/{slug}/plan.md with E2E scenarios and playwright-cli checks. Do NOT use for API/database work (feat-backend), full features (feat-feature), writing code (feat-exec) or UI review (feat-review).

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Route supported legacy PWDEV Code command intent to canonical PWDEV Flow skills. Use when the user mentions old /pwdev-code:* commands, asks for PWDEV Code compatibility, or wants to adopt an existing .planning workspace without losing history.

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Create an approval-gated product requirements document or decompose an approved PRD into a traceable roadmap. Use for product-level problems, new initiatives, multi-feature scope, PRDs, release outcomes, or roadmap planning.

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Use when the user wants to set up pwdev-feat in a project — 'inicializar o pwdev-feat', 'configurar o feat', 'init feat workspace' — choosing language, model profile and the optional audit trail, and creating the .planning/feat/ workspace. Do NOT use to analyze the codebase (feat-map), to write the project instructions file (feat-setup) or to create plans.

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