「sprs」の検索結果

Prepare a defense-contractor environment for CMMC Level 2 certification: scope CUI and FCI, implement the 110 NIST SP 800-171 Rev 2 security requirements across 14 families, compute the SPRS score with the DoD Assessment Methodology, manage a compliant POA&M, and ready the organization for a C3PAO assessment. Use when an organization handles Controlled Unclassified Information (CUI) under a DoD contract, when a contract carries DFARS clause 252.204-7012/7019/7020/7021, when preparing for or responding to a CMMC assessment, when computing or improving an SPRS score, when building a System Security Plan or POA&M for 800-171, or when scoping which systems are in the CUI boundary. Keywords: CMMC, CMMC Level 2, NIST 800-171, SP 800-171 Rev 2, CUI, FCI, SPRS, DFARS 7012, C3PAO, POA&M, System Security Plan, DoD Assessment Methodology, 110 controls, defense industrial base, DIB, FedRAMP equivalency.

日本語の概要は準備中です。原文の説明を表示しています。

Prepare a defense-contractor environment for CMMC Level 2 certification: scope CUI and FCI, implement the 110 NIST SP 800-171 Rev 2 security requirements across 14 families, compute the SPRS score with the DoD Assessment Methodology, manage a compliant POA&M, and ready the organization for a C3PAO assessment. Use when an organization handles Controlled Unclassified Information (CUI) under a DoD contract, when a contract carries DFARS clause 252.204-7012/7019/7020/7021, when preparing for or responding to a CMMC assessment, when computing or improving an SPRS score, when building a System Security Plan or POA&M for 800-171, or when scoping which systems are in the CUI boundary. Keywords: CMMC, CMMC Level 2, NIST 800-171, SP 800-171 Rev 2, CUI, FCI, SPRS, DFARS 7012, C3PAO, POA&M, System Security Plan, DoD Assessment Methodology, 110 controls, defense industrial base, DIB, FedRAMP equivalency.

日本語の概要は準備中です。原文の説明を表示しています。

Provides R wrappers of several on-target and off-target scoring methods for CRISPR guide RNAs (gRNAs). The following nucleases are supported: SpCas9, AsCas12a, enAsCas12a, and RfxCas13d (CasRx). The available on-target cutting efficiency scoring methods are RuleSet1, Azimuth, DeepHF, DeepCpf1, enPAM+GB, and CRISPRscan. Both the CFD and MIT scoring methods are available for off-target specificity prediction. The package also provides a Lindel-derived score to predict the probability of a gRNA to

日本語の概要は準備中です。原文の説明を表示しています。

Design guide RNAs for CRISPR-Cas9/Cas12a experiments using CRISPRscan and local scoring algorithms. Score guides for on-target activity using Rule Set 2 and Azimuth models. Use when designing sgRNAs for gene knockout, activation, or repression experiments.

日本語の概要は準備中です。原文の説明を表示しています。

Designs and ranks guide RNAs (sgRNAs) for CRISPR-Cas9/Cas12a gene knockout by scanning a target for PAM sites (NGG SpCas9, NNGRRT SaCas9, TTTV Cas12a, NG SpCas9-NG, near-PAMless SpRY), enumerating candidate spacers, applying hard filters (Pol-III TTTT terminator, 5' G, GC), ranking on-target activity with the context-appropriate model (Rule Set 2/Azimuth for U6/lentiviral, CRISPRscan for T7/embryo, DeepHF for high-fidelity variants, DeepCpf1 for Cas12a), and predicting the indel/frameshift outcome (Bae out-of-frame score, inDelphi, FORECasT, Lindel). Use when selecting sgRNAs to knock out a gene, choosing a nuclease/PAM for a constrained locus, picking which exon to target, or shortlisting guides before an off-target check. Off-target specificity, base/prime editing, and HDR donors are separate skills.

日本語の概要は準備中です。原文の説明を表示しています。

Expert CMMC 2.0 (Cybersecurity Maturity Model Certification) advisor for US defense contractors and subcontractors in the Defense Industrial Base (DIB). Use this skill whenever a user asks about CMMC 2.0, CMMC Level 1, Level 2, or Level 3, DoD cybersecurity compliance, NIST SP 800-171, CUI (Controlled Unclassified Information) protection, System Security Plan (SSP), Plan of Action & Milestones (POA&M), C3PAO assessments, DIBCAC audits, self-assessment, SPRS score, or any requirement under DFARS 252.204-7012 or 7021. Also trigger for: "CMMC gap analysis", "CMMC readiness", "FCI protection", "CUI scoping", "CMMC practices", "DoD contract cybersecurity", "defense supply chain security", "prime contractor flow-down requirements", the July 2026 CMMC Phase 2 suspension, or the CMMC Reform Task Force.

日本語の概要は準備中です。原文の説明を表示しています。

Expert CMMC 2.0 (Cybersecurity Maturity Model Certification) advisor for US defense contractors and subcontractors in the Defense Industrial Base (DIB). Use this skill whenever a user asks about CMMC 2.0, CMMC Level 1, Level 2, or Level 3, DoD cybersecurity compliance, NIST SP 800-171, CUI (Controlled Unclassified Information) protection, System Security Plan (SSP), Plan of Action & Milestones (POA&M), C3PAO assessments, DIBCAC audits, self-assessment, SPRS score, or any requirement under DFARS 252.204-7012 or 7021. Also trigger for: "CMMC gap analysis", "CMMC readiness", "FCI protection", "CUI scoping", "CMMC practices", "DoD contract cybersecurity", "defense supply chain security", or "prime contractor flow-down requirements".

日本語の概要は準備中です。原文の説明を表示しています。

Submission-grade remote-sensing Nature/high-impact journal figure workflow for Python or R. Use whenever the user asks to create, revise, audit, or polish manuscript figures, multi-panel scientific plots, map-led composites, remote-sensing image plates, sensor/product workflow figures, validation/error/uncertainty panels, or journal-ready SVG/PDF/TIFF outputs, especially for Nature-family, Remote Sensing of Environment, ISPRS, IEEE TGRS, or other high-impact venues. Before plotting, define the figure's conclusion, evidence logic, export needs, and review risks. If the user has not chosen Python or R, ask "Python or R?" and stop. Use only the selected backend for figure generation, previewing, exporting, and QA. Supports matplotlib/seaborn and ggplot2/patchwork/ComplexHeatmap. Not for dashboards or Illustrator/Figma-first infographics. Also trigger on general academic-writing figure needs even without the word "Nature", such as making figures/plots for a paper, scientific/academic plotting, data visualization for a manuscript, and Chinese phrasings like 遥感论文配图、学术写作配图、科研绘图、科研作图、地图出图、论文图表、可视化.

日本語の概要は準備中です。原文の説明を表示しています。

Designs and ranks guide RNAs (sgRNAs) for CRISPR-Cas9/Cas12a gene knockout by scanning a target for PAM sites (NGG SpCas9, NNGRRT SaCas9, TTTV Cas12a, NG SpCas9-NG, near-PAMless SpRY), enumerating candidate spacers, applying hard filters (Pol-III TTTT terminator, 5' G, GC), ranking on-target activity with the context-appropriate model (Rule Set 2/Azimuth for U6/lentiviral, CRISPRscan for T7/embryo, DeepHF for high-fidelity variants, DeepCpf1 for Cas12a), and predicting the indel/frameshift outcome (Bae out-of-frame score, inDelphi, FORECasT, Lindel). Use when selecting sgRNAs to knock out a gene, choosing a nuclease/PAM for a constrained locus, picking which exon to target, or shortlisting guides before an off-target check. Off-target specificity, base/prime editing, and HDR donors are separate skills.

日本語の概要は準備中です。原文の説明を表示しています。

Designs and ranks guide RNAs (sgRNAs) for CRISPR-Cas9/Cas12a gene knockout by scanning a target for PAM sites (NGG SpCas9, NNGRRT SaCas9, TTTV Cas12a, NG SpCas9-NG, near-PAMless SpRY), enumerating candidate spacers, applying hard filters (Pol-III TTTT terminator, 5' G, GC), ranking on-target activity with the context-appropriate model (Rule Set 2/Azimuth for U6/lentiviral, CRISPRscan for T7/embryo, DeepHF for high-fidelity variants, DeepCpf1 for Cas12a), and predicting the indel/frameshift outcome (Bae out-of-frame score, inDelphi, FORECasT, Lindel). Use when selecting sgRNAs to knock out a gene, choosing a nuclease/PAM for a constrained locus, picking which exon to target, or shortlisting guides before an off-target check. Off-target specificity, base/prime editing, and HDR donors are separate skills.

日本語の概要は準備中です。原文の説明を表示しています。