401 个 · 按相关度排序
Hunt for threats in AWS environments using Detective behavior graphs, entity investigation timelines, GuardDuty Bul:ing correlation, and automated entity profiling across IAM users, EC2 instances, and IP addresses.
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Build comprehensive threat actor profiles using open-source intelligence (OSINT) techniques to document adversary motivations, capabilities, infrastructure, and TTPs for proactive defense.
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Map advanced persistent threat (APT) group tactics, techniques, and procedures (TTPs) to the MITRE ATT&CK framework using the ATT&CK Navigator and attackcti Python library. The analyst queries STIX/TAXII data for group-technique associations, generates Navigator layer files for visualization, and compares defensive coverage against adversary profiles. Activates for requests involving APT TTP mapping, ATT&CK Navigator layers, threat actor profiling, or MITRE technique coverage analysis.
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Performance optimization, profiling, caching strategies, lazy loading, bundle optimization, and monitoring. Use when optimizing application performance, reducing bundle size, implementing caching, or analyzing bottlenecks.
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Profile a running web application's CPU performance using Cursor's built-in browser profiler. Captures call stacks, identifies slow functions, and suggests optimizations. Use when a page feels slow or janky.
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Explore and diagnose a PostHog endpoint's execution logs — error messages, failed runs, cache misses, slow runs, or unexpected row counts during endpoint invocations. Use when the user says "my endpoint is failing", "show me the logs for endpoint X", "what error did endpoint Y produce", "why did endpoint Z return no rows", "is this endpoint hitting cache", or "check the last N runs". Focused on a single named endpoint's runtime log entries, not project-wide auditing or query performance profiling.
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Visualize training metrics, debug models with histograms, compare experiments, visualize model graphs, and profile performance with TensorBoard - Google's ML visualization toolkit
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End-to-end Ribo-seq analysis from FASTQ through periodicity QC, P-site calibration, ORF detection, translation efficiency, and stalling. Use when orchestrating a full ribosome profiling pipeline and deciding harvest/dedup/alignment options and which downstream analyses the library can support.
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Drug-combination synergy analysis — quantify whether two drugs together are synergistic, additive, or antagonistic using the standard reference models (Bliss independence, HSA / highest single agent, Loewe additivity, ZIP, and the Chou-Talalay Combination Index). Use when you have measured single-drug and combination effects (inhibition/viability) and need a synergy score. Explains which model to use, what data each one needs, and how to read the score. NOT for looking up pre-computed synergy in a database (use the SYNERGxDB tool / cell-line-profiling skill).
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Comprehensive drug profiling — mechanism, primary/secondary targets, drug interactions, clinical-trial status, adverse events (FAERS), pharmacogenomics, and approval history. Use for full drug investigation reports, 'tell me about drug X' queries, and assembling drug profiles for clinicians, researchers, or regulatory work.
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Profiles a genome from raw reads BEFORE assembly with a k-mer spectrum (KMC or Jellyfish histogram), then models it with GenomeScope2 to estimate genome size, heterozygosity, repeat content, and ploidy, and Smudgeplot to infer ploidy from heterozygous k-mer pairs (diploid AB vs triploid AAB vs tetraploid AABB). Covers choosing k via Merqury best_k.sh, the k-mer-coverage vs sequencing-coverage confusion, reading het/repeat/contamination/organelle peaks, why noisy ONT must not be used for counting, and how the estimate becomes the NG50 denominator, the Flye -g value, the hifiasm --hom-cov/purge setting, and the 1.5-2x-too-big haplotig sanity check. Use when starting any de novo assembly, deciding whether short reads can work, estimating genome size for an unknown organism, diagnosing ploidy, or sanity-checking an assembly's size against expectation.
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Assembles a genome de novo from Illumina short reads with SPAdes (isolate/careful/sc/meta/plasmid/rna modes), MEGAHIT (low-memory, huge datasets), Unicycler (bacterial finishing/hybrid), MaSuRCA (large hybrid), ABySS (Bloom-filter), and Platanus (heterozygous diploids), using multi-k de Bruijn graphs. Covers the repeat-resolution limit, why N50 plateaus at the genome not the depth, GenomeScope2 k-mer profiling first, the heterozygosity/haplotig trap, error-correction erasing rare alleles, GC dropout, and NG50/auN/BUSCO reporting. Use when assembling a bacterial isolate, fungal, small-eukaryotic, single-cell, or metagenome genome from Illumina reads, or when deciding whether short reads can even produce the assembly being asked for.
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Identify direct miRNA-target interactions from AGO HITS-CLIP, AGO-CLEAR-CLIP (chimeric reads), HEAP (Halo-Ago2 mouse), chimeric eCLIP / miR-eCLIP (deep miRNA-target profiling), or CLASH using chimeric-read processing pipelines, seed-pairing analysis, and 3' auxiliary pairing rules. Use when distinguishing direct miRNA targets from indirect, integrating CLIP-derived target maps with TargetScan / miRDB / DIANA predictions, applying canonical 7mer-8mer seed matching with 3' UTR context, or recovering miRNA-mRNA chimeras at scale.
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Analyzes CUT&RUN (Skene Henikoff 2017) and CUT&Tag (Kaya-Okur 2019) chromatin profiling data. Handles SEACR vs MACS2 peak calling (with the btaf375 2025 benchmark guidance), pA-MNase vs pA-Tn5 vs pAG-Tn5 chimera differences, E. coli spike-in carryover normalization, IgG-only control logic (no input), characteristic fragment-size signatures (25-75 bp for CUT&Tag), and lower depth requirements (5M reads typical vs 25M for ChIP). Use when calling peaks from CUT&RUN/CUT&Tag, scaling by E. coli spike-in carryover, choosing SEACR norm mode, or comparing CUT&RUN/Tag results to traditional ChIP.
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Optimize end-to-end application performance with profiling, observability, and backend/frontend tuning. Use when coordinating performance optimization across the stack.
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Optimize multi-agent systems with coordinated profiling, workload distribution, and cost-aware orchestration. Use when improving agent performance, throughput, or reliability.
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Guides Rust performance optimization. Use when profiling, benchmarking, reducing allocations, improving cache locality, choosing between rayon/async/threads, or applying SIMD/parallelism.
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Audit and improve SwiftUI runtime performance from code review and architecture. Use for requests to diagnose slow rendering, janky scrolling, high CPU/memory usage, excessive view updates, or layout thrash in SwiftUI apps, and to provide guidance for user-run Instruments profiling when code review alone is insufficient.
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Measure, profile, and fix performance bottlenecks — Core Web Vitals, backend latency, bundle size, and database queries. Load when performance requirements exist, users report slowness, profiling reveals bottlenecks, or the user asks to optimize load time, LCP, INP, CLS, or response time. Not for premature optimization without evidence. Pairs with browser-testing-with-devtools and ci-cd-and-automation for regression guards.
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Test and debug browser UIs using Chrome DevTools MCP — DOM inspection, console errors, network requests, performance traces, and visual verification. Load when building or debugging anything that renders in a browser, verifying a UI fix, profiling Core Web Vitals in a real page, or the user asks for browser testing with DevTools. Requires chrome-devtools MCP configured. Not for backend-only or CLI work. Pairs with frontend-design and performance-optimization.
中文简介正在准备中。当前显示原文说明。
Optimize end-to-end application performance with profiling, observability, and backend/frontend tuning. Use when coordinating performance optimization across the stack.
中文简介正在准备中。当前显示原文说明。
Optimize multi-agent systems with coordinated profiling, workload distribution, and cost-aware orchestration. Use when improving agent performance, throughput, or reliability.
中文简介正在准备中。当前显示原文说明。
Assembles a genome de novo from Illumina short reads with SPAdes (isolate/careful/sc/meta/plasmid/rna modes), MEGAHIT (low-memory, huge datasets), Unicycler (bacterial finishing/hybrid), MaSuRCA (large hybrid), ABySS (Bloom-filter), and Platanus (heterozygous diploids), using multi-k de Bruijn graphs. Covers the repeat-resolution limit, why N50 plateaus at the genome not the depth, GenomeScope2 k-mer profiling first, the heterozygosity/haplotig trap, error-correction erasing rare alleles, GC dropout, and NG50/auN/BUSCO reporting. Use when assembling a bacterial isolate, fungal, small-eukaryotic, single-cell, or metagenome genome from Illumina reads, or when deciding whether short reads can even produce the assembly being asked for.
中文简介正在准备中。当前显示原文说明。
Profiles a genome from raw reads BEFORE assembly with a k-mer spectrum (KMC or Jellyfish histogram), then models it with GenomeScope2 to estimate genome size, heterozygosity, repeat content, and ploidy, and Smudgeplot to infer ploidy from heterozygous k-mer pairs (diploid AB vs triploid AAB vs tetraploid AABB). Covers choosing k via Merqury best_k.sh, the k-mer-coverage vs sequencing-coverage confusion, reading het/repeat/contamination/organelle peaks, why noisy ONT must not be used for counting, and how the estimate becomes the NG50 denominator, the Flye -g value, the hifiasm --hom-cov/purge setting, and the 1.5-2x-too-big haplotig sanity check. Use when starting any de novo assembly, deciding whether short reads can work, estimating genome size for an unknown organism, diagnosing ploidy, or sanity-checking an assembly's size against expectation.
中文简介正在准备中。当前显示原文说明。