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Integrate RuView through shell commands, an HTTP API, or WebAssembly builds, which run compiled code in browsers and supported edge devices. Covers survivor scan commands, result exports, API hosting, and browser or ESP32 deployment paths.
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Create and manage agents in WebAssembly, an execution environment that keeps their work isolated. Try untrusted code or agent configurations, manage sandbox files and tool access, and export agents for sharing.
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Migrate legacy AssemblyAI Streaming v2 and LeMUR integrations to Streaming v3 and LLM Gateway with parity evidence. Use when removing deprecated contracts. Trigger with "migrate AssemblyAI", "LeMUR migration", or "Streaming v3 upgrade".
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Produce a redacted AssemblyAI diagnostic bundle for support and incident triage. Use when evidence is needed without exposing keys, URLs, audio, transcripts, or prompts. Trigger with "AssemblyAI debug bundle" or "support evidence".
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Analyze and gate an AssemblyAI integration across contracts, security, reliability, cost, observability, retention, and rollback. Use when preparing a launch or material change. Trigger with "AssemblyAI production checklist" or "go live".
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Analyze and triage AssemblyAI REST, transcript, Streaming v3, webhook, and LLM Gateway failures without leaking customer content. Use when an integration errors or stalls. Trigger with "AssemblyAI error" or "failed transcript".
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Analyze and operate a production AssemblyAI pre-recorded pipeline with explicit models, Speech Understanding, callbacks, and retention controls. Use when running asynchronous transcription. Trigger with "AssemblyAI async transcription" or "pre-recorded pipeline".
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Build AssemblyAI CI with offline REST, Streaming v3, webhook, gateway, secret, and lifecycle tests plus a tightly gated live lane. Use when adding automated verification. Trigger with "AssemblyAI CI" or "contract test".
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PCBWay PCB fabrication and assembly — turnkey/consigned assembly, design rules, ordering workflow. Alternative to JLCPCB for manufacturing. Use with KiCad. Use this skill when the user mentions PCBWay, needs turnkey assembly (PCBWay sources parts by MPN), has parts not available on LCSC, needs assembled boards with non-LCSC components, wants to compare PCBWay vs JLCPCB, or needs assembly with parts sourced globally rather than from LCSC only. For gerber/CPL export, stencil ordering, and BOM management, see the `bom` skill.
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PCBWay PCB fabrication and assembly — turnkey/consigned assembly, design rules, ordering workflow. Alternative to JLCPCB for manufacturing. Use with KiCad. Use this skill when the user mentions PCBWay, needs turnkey assembly (PCBWay sources parts by MPN), has parts not available on LCSC, needs assembled boards with non-LCSC components, wants to compare PCBWay vs JLCPCB, or needs assembly with parts sourced globally rather than from LCSC only. For gerber/CPL export, stencil ordering, and BOM management, see the `bom` skill.
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Profiles a genome from raw reads BEFORE assembly with a k-mer spectrum (KMC or Jellyfish histogram), then models it with GenomeScope2 to estimate genome size, heterozygosity, repeat content, and ploidy, and Smudgeplot to infer ploidy from heterozygous k-mer pairs (diploid AB vs triploid AAB vs tetraploid AABB). Covers choosing k via Merqury best_k.sh, the k-mer-coverage vs sequencing-coverage confusion, reading het/repeat/contamination/organelle peaks, why noisy ONT must not be used for counting, and how the estimate becomes the NG50 denominator, the Flye -g value, the hifiasm --hom-cov/purge setting, and the 1.5-2x-too-big haplotig sanity check. Use when starting any de novo assembly, deciding whether short reads can work, estimating genome size for an unknown organism, diagnosing ploidy, or sanity-checking an assembly's size against expectation.
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Binary analysis, assembly interpretation, disassembly, decompilation, firmware RE, and protocol reverse engineering
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Polish genome assemblies to reduce errors using short reads (Pilon), long reads (Racon), or ONT-specific tools (medaka). Essential for improving long-read assembly accuracy. Use when improving assembly accuracy with polishing tools.
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PCBWay PCB fabrication and assembly — turnkey/consigned assembly, design rules, ordering workflow. Alternative to JLCPCB for manufacturing. Use with KiCad. Use this skill when the user mentions PCBWay, needs turnkey assembly (PCBWay sources parts by MPN), has parts not available on LCSC, needs assembled boards with non-LCSC components, wants to compare PCBWay vs JLCPCB, or needs assembly with parts sourced globally rather than from LCSC only. For gerber/CPL export, stencil ordering, and BOM management, see the `bom` skill.
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Orchestrates an end-to-end de novo genome assembly project, routing each step to the right genome-assembly skill rather than restating it. Profiles the genome first (k-mer spectrum -> size, heterozygosity, ploidy), QCs reads, chooses an assembly path by data type (SPAdes for Illumina, Flye for noisy long reads, hifiasm for HiFi, metaFlye for communities), polishes only when needed, decontaminates, scaffolds with Hi-C, and finishes with three-axis QC (contiguity + completeness + correctness). Use when assembling a genome from raw reads and deciding which assembler, whether to polish, and how to prove the result is good.
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Profiles a genome from raw reads BEFORE assembly with a k-mer spectrum (KMC or Jellyfish histogram), then models it with GenomeScope2 to estimate genome size, heterozygosity, repeat content, and ploidy, and Smudgeplot to infer ploidy from heterozygous k-mer pairs (diploid AB vs triploid AAB vs tetraploid AABB). Covers choosing k via Merqury best_k.sh, the k-mer-coverage vs sequencing-coverage confusion, reading het/repeat/contamination/organelle peaks, why noisy ONT must not be used for counting, and how the estimate becomes the NG50 denominator, the Flye -g value, the hifiasm --hom-cov/purge setting, and the 1.5-2x-too-big haplotig sanity check. Use when starting any de novo assembly, deciding whether short reads can work, estimating genome size for an unknown organism, diagnosing ploidy, or sanity-checking an assembly's size against expectation.
中文简介正在准备中。当前显示原文说明。
Profiles a genome from raw reads BEFORE assembly with a k-mer spectrum (KMC or Jellyfish histogram), then models it with GenomeScope2 to estimate genome size, heterozygosity, repeat content, and ploidy, and Smudgeplot to infer ploidy from heterozygous k-mer pairs (diploid AB vs triploid AAB vs tetraploid AABB). Covers choosing k via Merqury best_k.sh, the k-mer-coverage vs sequencing-coverage confusion, reading het/repeat/contamination/organelle peaks, why noisy ONT must not be used for counting, and how the estimate becomes the NG50 denominator, the Flye -g value, the hifiasm --hom-cov/purge setting, and the 1.5-2x-too-big haplotig sanity check. Use when starting any de novo assembly, deciding whether short reads can work, estimating genome size for an unknown organism, diagnosing ploidy, or sanity-checking an assembly's size against expectation.
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Find prebuilt WASM agents, software components packaged in WebAssembly format, through gallery browsing and keyword search. Install agents from their configurations, or export and publish your own agents to share with the community.
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Analyze compiled programs without running them by recognizing branches, loops, data structures, and common instruction patterns. Use disassembly and decompilation, which translate machine code into readable representations, to reconstruct program logic and document findings.
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Investigate and fix agent behavior in TradingAgentsCN by checking active database prompt templates, prompt assembly code, and output processing. Apply targeted changes and verify them with focused tests, template checks, and workflow logs.
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Retrieve focused genome, assembly, and taxonomy metadata from NCBI Datasets v2, a biological data service. Get concise Markdown summaries by default, with raw JSON or text available when requested.
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Assembles a genome de novo from Illumina short reads with SPAdes (isolate/careful/sc/meta/plasmid/rna modes), MEGAHIT (low-memory, huge datasets), Unicycler (bacterial finishing/hybrid), MaSuRCA (large hybrid), ABySS (Bloom-filter), and Platanus (heterozygous diploids), using multi-k de Bruijn graphs. Covers the repeat-resolution limit, why N50 plateaus at the genome not the depth, GenomeScope2 k-mer profiling first, the heterozygosity/haplotig trap, error-correction erasing rare alleles, GC dropout, and NG50/auN/BUSCO reporting. Use when assembling a bacterial isolate, fungal, small-eukaryotic, single-cell, or metagenome genome from Illumina reads, or when deciding whether short reads can even produce the assembly being asked for.
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Assembles microbial-community sequencing into metagenome-assembled genomes (MAGs) with metaFlye (ONT), metaSPAdes/MEGAHIT (Illumina), and hifiasm-meta/metaMDBG (PacBio HiFi), then recovers genomes via multi-binner consolidation (MetaBAT2, MaxBin2, CONCOCT, SemiBin2, VAMB -> DAS_Tool) and QCs them against MIMAG with CheckM2, GUNC, and GTDB-Tk. Covers why a metagenome is not a genome (uneven coverage, micro-diversity, strain collapse to consensus), differential-coverage binning, co-assembly vs per-sample, the rRNA-operon collapse that fails short-read MAGs, and strain resolution with inStrain. Use when reconstructing genomes from a microbiome, soil, ocean, or gut community, recovering MAGs, or resolving strain-level variation.
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Detects and removes contamination in genome assemblies via two disjoint workflows - foreign-sequence screening of a single-organism (eukaryote/isolate) assembly with NCBI FCS-GX (GenBank-submission-mandatory), FCS-adaptor, and BlobToolKit blob plots; and MAG/bin quality assessment with CheckM2 plus GUNC (chimerism) plus GTDB-Tk taxonomy, judged against MIMAG. Covers why CheckM2 alone is blind to disjoint-marker chimeras, the FCS-GX RAM wall, organelle/NUMT triage, strain heterogeneity, and the HGT-vs-contamination (tardigrade) trap. Use when screening an assembly for foreign contamination before GenBank submission, assessing MAG completeness/contamination/chimerism, deciding which contigs to remove, or distinguishing real HGT from contaminant contigs.
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