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Run remote BLAST searches against NCBI servers using Biopython Bio.Blast.NCBIWWW. Use when identifying unknown sequences, finding homologs, picking the correct BLAST program (blastn/blastp/blastx/tblastn/tblastx/psiblast/megablast/dc-megablast), interpreting Karlin-Altschul E-values, avoiding the max_target_seqs trap (Shah 2019), choosing composition-based statistics, or limiting searches by organism. Covers RID lifecycle, database choice (nt/nr/refseq_select/swissprot), word-size and CBS taxonomy.

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Run remote BLAST searches against NCBI servers using Biopython Bio.Blast.NCBIWWW. Use when identifying unknown sequences, finding homologs, picking the correct BLAST program (blastn/blastp/blastx/tblastn/tblastx/psiblast/megablast/dc-megablast), interpreting Karlin-Altschul E-values, avoiding the max_target_seqs trap (Shah 2019), choosing composition-based statistics, or limiting searches by organism. Covers RID lifecycle, database choice (nt/nr/refseq_select/swissprot), word-size and CBS taxonomy.

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Run remote BLAST searches against NCBI servers using Biopython Bio.Blast.NCBIWWW. Use when identifying unknown sequences, finding homologs, picking the correct BLAST program (blastn/blastp/blastx/tblastn/tblastx/psiblast/megablast/dc-megablast), interpreting Karlin-Altschul E-values, avoiding the max_target_seqs trap (Shah 2019), choosing composition-based statistics, or limiting searches by organism. Covers RID lifecycle, database choice (nt/nr/refseq_select/swissprot), word-size and CBS taxonomy.

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Run remote BLAST searches against NCBI servers using Biopython Bio.Blast.NCBIWWW. Use when identifying unknown sequences, finding homologs, picking the correct BLAST program (blastn/blastp/blastx/tblastn/tblastx/psiblast/megablast/dc-megablast), interpreting Karlin-Altschul E-values, avoiding the max_target_seqs trap (Shah 2019), choosing composition-based statistics, or limiting searches by organism. Covers RID lifecycle, database choice (nt/nr/refseq_select/swissprot), word-size and CBS taxonomy.

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Run remote BLAST searches against NCBI servers using Biopython Bio.Blast.NCBIWWW. Use when identifying unknown sequences, finding homologs, picking the correct BLAST program (blastn/blastp/blastx/tblastn/tblastx/psiblast/megablast/dc-megablast), interpreting Karlin-Altschul E-values, avoiding the max_target_seqs trap (Shah 2019), choosing composition-based statistics, or limiting searches by organism. Covers RID lifecycle, database choice (nt/nr/refseq_select/swissprot), word-size and CBS taxonomy.

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Set up Python APIs with FastAPI using organized project templates. Build routes, asynchronous database operations, authentication, and error handling, with examples for sharing dependencies and testing endpoints through a separate test database.

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• Set up a new FastAPI project
• Build a Python API with asynchronous database access
• Organize authentication and shared dependencies

Build a GitLab CI/CD pipeline with automated checks for vulnerable code, dependencies, container images, running applications, and exposed secrets. Covers scanner configuration, consolidated findings, and approval policies for serious vulnerabilities before production deployment.

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• Add automated security scans to .gitlab-ci.yml
• Check dependencies and container images for known vulnerabilities
• Detect credentials and API keys committed to source control

Launch machine learning experiments on local GPUs, remote servers, Vast.ai, or Modal. It checks the configured environment, deploys code, saves logs, and verifies startup, with optional W&B metric logging and automatic Vast.ai result collection and instance cleanup.

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• Run training jobs on a local GPU
• Deploy experiments to an SSH server
• Run experiments on Vast.ai and collect results

Validate Illumina BCL run folders and sample sheets, plan demultiplexing—the separation of reads by sample—and convert data to FASTQ using installed tools. Review conversion metrics, index settings, and reads that could not be assigned.

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• Checking run folders and sample sheets before conversion
• Reviewing index, UMI, and lane settings
• Converting Illumina BCL data to FASTQ

Build local BLAST databases and run searches using NCBI BLAST+ command-line tools. Use when running >50 queries, building custom databases with -parse_seqids and -taxid, downloading prebuilt NCBI databases via update_blastdb.pl, choosing -task variants (megablast/dc-megablast/blastn/blastn-short), tuning soft/hard masking, scaling threads, or extracting hits with blastdbcmd. Encodes BLAST v5 vs v4 database format, taxonomy filtering, makeblastdb pitfalls.

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When the user wants to plan, create, or market a podcast. Also use when the user mentions "podcast," "podcast strategy," "podcast SEO," "show notes," "podcast distribution," "Spotify podcast," "Apple Podcasts," "podcast discoverability," "PodcastEpisode schema," or "podcast repurposing." For show notes, use article-content.

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Comprehensive social media operations for B2B SaaS brand building and engagement. Use this skill to develop LinkedIn strategy, decide whether to run a LinkedIn newsletter and how to scope it, capture consented registrations from LinkedIn Events, check LinkedIn Live eligibility, manage Twitter/X presence, create YouTube content, engage on Reddit communities, decide whether and how to answer on Quora, build community, develop influencer and creator partnerships, vet a creator before paying them, start or fix a B2B podcast, book podcast guests and guest appearances, judge whether a podcast is worth appearing on when you cannot audit its numbers, write show notes and transcripts, run social listening (what are people saying about us, share of voice, brand monitoring, validated sentiment, alert thresholds, routing signals to the right owner), and execute organic social campaigns. Also triggers on: LinkedIn, LinkedIn newsletter, LinkedIn Events, LinkedIn Live, event registration form, subscribe to our newsletter on LinkedIn, marketing consent checkbox, Notify Employees, Twitter/X, YouTube, Reddit, community, influencer, creator partnerships, influencer vetting, engagement rate, fake followers, engagement pods, FTC disclosure, sponsored post disclosure, media kit, social media strategy, organic social, employee advocacy, podcast, podcast strategy, podcast guesting, how do I know if this podcast has real listeners, the host says they get 50k downloads, is this podcast worth going on, podcast RSS feed research, IAB podcast measurement certification, Listen Score, Apple Podcasts reviews, show notes, audiogram, episode transcript, social media account access, who has admin on our LinkedIn page, the person who ran our social accounts left, locked out of our company account, social media offboarding, YouTube channel owner, Slack primary owner, social account MFA, social media approval workflow, pause all scheduled posts, social listening, what are people saying about us, brand monitoring, brand mentions, share of voice, social share of voice vs competitors, sentiment analysis, is our sentiment dashboard accurate, listening query, boolean query, mention alerts, spike alert, CrowdTangle replacement, Reddit API access, which platforms can our listening tool see, someone on Reddit asked for a tool like ours, comment mining, voice of customer from social.

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Build local BLAST databases and run searches using NCBI BLAST+ command-line tools. Use when running >50 queries, building custom databases with -parse_seqids and -taxid, downloading prebuilt NCBI databases via update_blastdb.pl, choosing -task variants (megablast/dc-megablast/blastn/blastn-short), tuning soft/hard masking, scaling threads, or extracting hits with blastdbcmd. Encodes BLAST v5 vs v4 database format, taxonomy filtering, makeblastdb pitfalls.

中文简介正在准备中。当前显示原文说明。

Build local BLAST databases and run searches using NCBI BLAST+ command-line tools. Use when running >50 queries, building custom databases with -parse_seqids and -taxid, downloading prebuilt NCBI databases via update_blastdb.pl, choosing -task variants (megablast/dc-megablast/blastn/blastn-short), tuning soft/hard masking, scaling threads, or extracting hits with blastdbcmd. Encodes BLAST v5 vs v4 database format, taxonomy filtering, makeblastdb pitfalls.

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Retrieve World Monitor forecasts to review scenario probabilities, changes, and supporting signals by region or topic. Check forecast accuracy records with their audit status, and distinguish unavailable data from an absence of forecasted risks.

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• Review conflict forecasts for a specific region
• Check changes in scenario probabilities
• Inspect signals behind a forecast

Review YouTube titles, thumbnails, openings, and pacing through a framework drawn from MrBeast’s public statements and other research. Use click-through rates and viewing data to identify weaknesses and develop specific changes to a video’s presentation and structure.

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• Reworking video titles and thumbnails
• Improving the first 30 seconds of a video
• Reviewing scripts for viewer drop-off

Integrate new FastGPT resources, such as AgentSkill or Plugin, with permission management. Choose an integration guide based on resource hierarchy and permission inheritance, locate the relevant code, and review a checklist for missing implementation steps.

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• Adding permission support to a new FastGPT resource
• Choosing an integration path based on permission inheritance
• Finding code locations for permission definitions and checks

Run smoke tests, or basic operational checks, for Mastra projects locally or in staging and production. Verify Studio screens, agents, tools, workflows, traces, and memory, then report results, blockers, and skipped checks.

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• Checking a local Mastra project before release
• Testing Studio and server deployments in the cloud
• Running targeted checks for agents or traces

Convert suitable diagrams in Mastra documentation into Mermaid, a text format for diagrams, or create new ones. Assess which images to keep, follow diagram styling rules, and verify layout and readability in light and dark modes.

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• Replace box-and-arrow images with Mermaid diagrams
• Add a new diagram to Mastra documentation
• Fix diagram readability in light and dark modes

Audits Mastra documentation against repository source and canonical writing guidance. Checks examples in context, API reference coverage, and targeted automated results, then produces an evidence-based report with findings and limitations without editing files.

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• Auditing every changed documentation page in a pull request
• Checking code examples against source and surrounding explanations
• Finding missing or incorrect API reference details

Build Python APIs with FastAPI and Pydantic V2, including input validation, database operations that avoid blocking other work, and token-based authentication. Implement REST or WebSocket endpoints, then check their behavior with automated tests and generated API documentation.

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• Build REST APIs with FastAPI
• Validate input data with Pydantic V2
• Implement JWT authentication and authorization

DevOps 与站点可靠性工程 (SRE) — 平台 / 基础设施 / 可靠性工程师的认知操作系统, 覆盖软件交付 + 运维全生命周期 (CI/CD 与发布工程 trunk-based + 渐进式发布 canary/blue-green/feature flag + GitOps Argo CD/Flux / 基础设施即代码 Terraform/OpenTofu/Pulumi/Ansible + policy-as-code OPA / 容器与编排 Docker/Kubernetes + Helm/Kustomize + service mesh Istio/Linkerd / 可观测性 Prometheus + Loki + OpenTelemetry + Honeycomb + eBPF + RED/USE / SLO-SLI-error budget 与可靠性工程 Google SRE 学科 + 容量规划 + 优雅降级 / 事件管理与 on-call 事件指挥 + PagerDuty + runbook + 无指责复盘 + MTTR / 云平台与 FinOps AWS/GCP/Azure + 成本优化 + 弹性伸缩 / 平台工程与开发者体验 IDP + Backstage + golden path + Team Topologies / DevSecOps 与供应链安全 shift-left + SBOM + SLSA + sigstore + Vault / 韧性与混沌工程 fault injection + game day + 安全科学 / DORA 指标与工程效能 部署频率 + 变更前置时间 + 变更失败率 + Accelerate 研究 / 数据库与有状态运维 schema 迁移 + 备份容灾) — 不含 通用应用开发 / 纯云销售认证速成 / 'DevOps = 跑 Jenkins 的岗位' 窄化误解 / ITIL 工单文化传统运维 (旧范式仅做边界) / 把手工运维 ClickOps 当稳态 (是 toil, 本 skill 核心反模式) (DevOps & Site Reliability Engineering — the cognitive operating system of platform / infrastructure / reliability practitioners who own the full software delivery + operational lifecycle, covering (a) CI/CD & release engineering (build pipelines, trunk-based development, progressive delivery — canary / blue-green / feature flags, GitOps with Argo CD / Flux), (b) Infrastructure as Code (Terraform / OpenTofu, Pulumi, CloudFormation, Ansible, Crossplane — module design, state management, drift, policy-as-code OPA / Sentinel / Checkov), (c) containers & orchestration (Docker / OCI, Kubernetes — scheduling, networking CNI, storage CSI, operators / CRDs, Helm / Kustomize, service mesh Istio / Linkerd), (d) observability (the three pillars + beyond — metrics Prometheus / VictoriaMetrics, logs Loki / ELK, traces OpenTelemetry / Jaeger / Tempo, high-cardinality observability Honeycomb, eBPF, RED / USE methods, SLO-based alerting), (e) SLO / SLI / error budgets & reliability engineering (Google SRE discipline — service level objectives, error budget policy, toil budgets, capacity planning, load shedding, graceful degradation), (f) incident management & on-call (incident command, PagerDuty / Opsgenie, runbooks, blameless postmortems, MTTR / MTTD, error budget burn), (g) cloud platforms & FinOps (AWS / GCP / Azure well-architected, multi-region, cost optimization, autoscaling), (h) platform engineering & developer experience (internal developer platforms, Backstage, golden paths, self-service, Team Topologies), (i) DevSecOps & supply-chain security (shift-left, SAST / DAST, SBOM, SLSA, sigstore / cosign, secrets management Vault), (j) resilience & chaos engineering (chaos experiments, fault injection, game days, resilience engineering / safety science), (k) DORA metrics & engineering effectiveness (deployment frequency, lead time, change failure rate, MTTR, the Accelerate research), (l) databases & stateful operations (schema migrations, backups / DR, replication); NOT generic software development / app feature coding (是 平行学科, DevOps/SRE 关注 delivery + operability 不是 product feature), NOT pure cloud sales / certification cram without operational depth, NOT 'DevOps = a job title that runs Jenkins' 的窄化误解 (DevOps 是 文化 + 实践, SRE 是 Google 对 reliability 的工程化具体实现), NOT ITIL-heavy 传统运维 工单文化 (是 被 DevOps 取代的旧范式, 仅做边界标注), NOT manual ops / ClickOps as a steady state (是 toil, 本 skill 的核心反模式).) Master OS — automated mastery of DevOps & Site Reliability Engineering — the cognitive operating system of platform / infrastructure / reliability practitioners who own the full software delivery + operational lifecycle, covering (a) CI/CD & release engineering (build pipelines, trunk-based development, progressive delivery — canary / blue-green / feature flags, GitOps with Argo CD / Flux), (b) Infrastructure as Code (Terraform / OpenTofu, Pulumi, CloudFormation, Ansible, Crossplane — module design, state management, drift, policy-as-code OPA / Sentinel / Checkov), (c) containers & orchestration (Docker / OCI, Kubernetes — scheduling, networking CNI, storage CSI, operators / CRDs, He

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Launch a podcast or plan podcast guest strategy — format, content planning, episode production, guest outreach, and distribution. Use when "podcast", "start a podcast", "podcast marketing", "podcast guest", "podcast strategy", "be a podcast guest", "launch a show", or any request related to podcast creation or guest appearances.

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Review form fields for code that blocks pasting, then remove those restrictions. The skill helps check password manager use, keyboard interactions, and accessibility so people can paste passwords and other long values into inputs.

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• Checking why passwords cannot be pasted
• Reviewing credit card fields for paste restrictions
• Improving form accessibility