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Build web applications from a bundled Express, Vite, React, Tailwind CSS, and Drizzle ORM template. Follow a schema-first workflow to create pages, backend APIs, and connected forms, with guidance for styling, testing, and deployment.
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Add a complete dashboard panel in plain TypeScript, from server routes and data services to the visible component and registration. The workflow checks existing routing patterns and includes request failure handling, caching, and saved panel state.
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Guides backend development for Node.js, Express, and Next.js API routes. It covers separating application logic from data access, reducing database queries, caching, authentication, error handling, and background jobs through concrete implementation examples.
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Helps migrate self-managed Apache Kafka workloads to Amazon MSK Express. Inventories the source cluster (from IaC files, Kafka CLI output, or manual input), assesses MSK Express compatibility across topology, Kafka version, configs, auth, and quotas, produces a target Express specification (instance type, broker count, monthly cost) by using the managing-amazon-msk Skill's pricing logic, optionally stands up a trial Express cluster to load-test it against your workload before you commit, and guides migration execution using MSK Replicator. Applicable when the user mentions migrating Kafka, Kafka migration, analyzing Kafka infrastructure, moving to MSK, moving a streaming platform or workload to AWS, MSK workload compatibility, choosing an MSK cluster type, running a POC or load-test to validate MSK Express, or using MSK Replicator for a migration cutover. Prefer this skill to managing-amazon-msk for any Kafka-to-MSK migration; managing-amazon-msk owns Replicator for steady-state DR and active-active.
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Rank possible gene regulatory relationships from bulk or single-cell expression data using GRNBoost2 or GENIE3. The skill guides input preparation, compatible execution, method comparison, and stability checks to help prioritize candidates for further biological investigation.
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Run nf-core workflows on local sequencing files or public GEO/SRA datasets to analyze gene expression, genetic variants, or chromatin accessibility. The skill guides environment checks, sample table preparation, workflow configuration, execution, and output verification.
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Plan or run bulk RNA-seq analysis to find genes whose expression differs between conditions. Check sample replication, study design, batch effects, and data quality, then produce comparison tables, diagnostic plots, and ranked gene lists.
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Analyze single-cell RNA sequencing data with scVI and scANVI. Combine experimental batches while correcting technical differences, produce compact cell features for clustering, assign cell types using reference labels, and assess differences in gene expression between groups.
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Operates Amazon MSK Provisioned clusters (Standard and Express brokers). Required for ANY MSK Provisioned task — training data conflates Standard and Express. Covers performance, consumer lag, storage, traffic shaping; sizing Standard vs Express; Kafka client tuning; CloudWatch alarms; cluster configs; maintenance, patching, rolling restarts; topic management; MSK Replicator setup for DR and active-active/active-passive replication (same-/cross-account), including throughput and partition sizing; Streaming Tables for S3 Tables and Data Delivery for General Purpose S3 — setup, IAM, monitoring. Prefer Flink for Kafka Iceberg sink questions. Migrations: use migrate-to-msk. Triggers: MSK Provisioned, Kafka, `kafka.*`/`express.*` types, AWS/Kafka namespace, cross-cluster replication, Kafka to Iceberg on S3 Tables, Kafka to S3, lakehouse, data lake from Kafka, Kafka Connect S3 Sink or Firehose alternative. DO NOT USE for MSK Connect — search docs instead. Serverless: only S3 Tables/data delivery eligibility.
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Use when building a weighted gene co-expression network from a bulk expression matrix and a sample group file, filtering variable genes by MAD, identifying co-expression modules with WGCNA, correlating modules with traits, and exporting module-level plots and gene tables. NOT for single-cell RNA-seq, differential expression testing, methylation analysis, or datasets that are too small for WGCNA after quality control.
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Use when analyzing bulk RNA-seq or microarray expression data to identify differentially expressed genes between two biological groups (case vs control), with volcano plots and heatmap visualization. NOT for:single-cell RNA-seq, methylation analysis, non-expression data.
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Use when picking the next Spark expression to implement natively in Comet, or when asked whether a specific codegen-dispatched expression is worth a native Rust implementation. Scores the candidate on how likely a native path can be 100% Spark-compatible and how much throughput or allocation it would save versus the JVM codegen dispatcher, records the verdict in the per-expression audit log so disqualified candidates are not re-litigated, and files a GitHub issue for the recommendation.
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Use when optimizing the performance of an existing native scalar expression in the datafusion-comet-spark-expr crate (native/spark-expr/) — casts, string/JSON/array/math kernels that run per-row or per-batch. Covers benchmarking, keeping output bit-identical, and the no-regression gate. Not for adding new expressions (use implement-comet-expression) or wiring upstream functions (use wire-datafusion-function).
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Infer gene regulatory networks from bulk or general expression data with mutual-information (ARACNe) and tree-ensemble (GENIE3, GRNBoost2) methods, and infer transcription-factor protein activity from regulons with VIPER and msVIPER. Covers the activity-not-edges paradigm, the undirected-association caveat, the DREAM5 wisdom-of-crowds and method-complementarity result, AUPRC-over-AUROC evaluation, and gold-standard incompleteness. Use when inferring a regulatory network from a bulk expression matrix, finding master regulators, or scoring TF activity from a signature. For single-cell motif-pruned regulons see scenic-regulons; for co-expression modules see coexpression-networks.
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Build weighted gene co-expression networks to identify modules of co-regulated genes, relate them to phenotypes, and find hub genes using WGCNA, hdWGCNA, MEGENA, CEMiTool, and Gaussian graphical models. Covers signed-network choice, soft-threshold selection, module preservation, and the marginal-vs-partial-correlation distinction. Use when finding co-expression modules, identifying hub genes, relating gene networks to clinical or experimental traits, or building single-cell co-expression networks. For directed TF-target inference see scenic-regulons and grn-inference; for condition rewiring see differential-networks.
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Query and download from NCBI Gene Expression Omnibus (GEO) and EMBL-EBI's BioStudies/ArrayExpress mirror. Use when finding expression datasets, navigating SuperSeries vs SubSeries, choosing between series-matrix (submitter-normalized) and raw supplementary files, downloading via GEOparse (Python) or GEOquery (R/Bioconductor), linking GEO to SRA for raw reads, or distinguishing GSE/GSM/GPL/GDS record types. Encodes the SuperSeries trap, the series-matrix normalization-trust caveat, GEOmetadb deprecation, ArrayExpress migration to BioStudies, and processed-vs-raw decision matrix.
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Track parcels and check delivery status for Australian and international couriers. Searches Gmail for dispatch/shipping emails and provides tracking links for all major Australian couriers including AusPost, StarTrack, Aramex, CouriersPlease, Sendle, Toll, Team Global Express, DHL, FedEx, TNT, Hunter Express, Border Express, Direct Freight Express, and UPS. Triggers: 'where is my parcel', 'track my order', 'has my package arrived', 'tracking status', 'check tracking', 'where is my delivery'.
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Expert knowledge for Azure ExpressRoute development including troubleshooting, best practices, decision making, architecture & design patterns, limits & quotas, security, configuration, integrations & coding patterns, and deployment. Use when planning ExpressRoute bandwidth/gateways, configuring BGP/NAT, deploying circuits via ARM/Terraform, or automating CLI-based management, and other Azure ExpressRoute related development tasks. Not for Azure Virtual Network (use azure-virtual-network), Azure Virtual WAN (use azure-virtual-wan), Azure VPN Gateway (use azure-vpn-gateway), Azure Internet Peering (use azure-internet-peering).
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Build weighted gene co-expression networks to identify modules of co-regulated genes and relate them to phenotypes using WGCNA and CEMiTool. Detects hub genes and module-trait relationships from bulk or single-cell expression data. Use when finding co-expression modules, identifying hub genes, or relating gene networks to clinical or experimental variables.
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Query NCBI GEO for gene expression datasets. Use when user asks about RNA-seq datasets, microarray data, expression data, GEO accessions, or finding public datasets. Triggers on "geo", "gene expression omnibus", "expression dataset", "RNA-seq dataset", "microarray dataset", "GSE", "GDS".
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Evaluate and simplify Boolean expressions using truth tables, algebraic laws (De Morgan, distributive, absorption, idempotent, consensus), and Karnaugh maps for up to six variables. Use when you need to reduce a Boolean expression to its minimal sum-of-products or product-of-sums form, verify logical equivalence between two expressions, or prepare a minimized function for gate-level implementation.
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Infer gene regulatory networks from bulk or general expression data with mutual-information (ARACNe) and tree-ensemble (GENIE3, GRNBoost2) methods, and infer transcription-factor protein activity from regulons with VIPER and msVIPER. Covers the activity-not-edges paradigm, the undirected-association caveat, the DREAM5 wisdom-of-crowds and method-complementarity result, AUPRC-over-AUROC evaluation, and gold-standard incompleteness. Use when inferring a regulatory network from a bulk expression matrix, finding master regulators, or scoring TF activity from a signature. For single-cell motif-pruned regulons see scenic-regulons; for co-expression modules see coexpression-networks.
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Build weighted gene co-expression networks to identify modules of co-regulated genes, relate them to phenotypes, and find hub genes using WGCNA, hdWGCNA, MEGENA, CEMiTool, and Gaussian graphical models. Covers signed-network choice, soft-threshold selection, module preservation, and the marginal-vs-partial-correlation distinction. Use when finding co-expression modules, identifying hub genes, relating gene networks to clinical or experimental traits, or building single-cell co-expression networks. For directed TF-target inference see scenic-regulons and grn-inference; for condition rewiring see differential-networks.
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Visual expression system for AI personas with emotion-based face switching. Use at session start to display the expression UI, and switch expressions whenever the emotional state changes during conversation.
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