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Configures AWS Site-to-Site VPN: creating an IPsec VPN connection between an on-premises network and a VPC, choosing the target gateway (virtual private gateway, transit gateway, or AWS Cloud WAN), choosing static or dynamic (BGP) routing, sizing tunnel bandwidth (Standard 1.25 Gbps or Large 5 Gbps), connecting many sites through a VPN Concentrator, applying the customer gateway device configuration, making a connection highly available, and monitoring tunnels with CloudWatch. Applicable when the user wants to connect a data center or branch office to AWS over an encrypted tunnel, choose how routes are exchanged, scale throughput, consolidate sites, or diagnose a down tunnel. Routes to the right per-task procedure in references. Not for AWS Direct Connect (its own service), Client VPN for individual remote users, the transit gateway side of a VPN attachment (transitgateway skill), or Route 53 DNS work.

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Query protein-protein and gene interaction databases (STRING, BioGRID, IntAct, SIGNOR, Reactome, HuRI, HuMAP, OmniPath, ConsensusPathDB, DIP). Use when building PPI networks, choosing between physical vs functional vs genetic interactions, signed/directed vs undirected, high-throughput vs curated, picking confidence thresholds, aggregating across resources, or navigating license constraints. Encodes the database decision matrix, STRING v12 channel semantics, OmniPath as meta-database, SIGNOR for signed signaling, and per-resource rate limits.

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Deploy and manage vLLM for high-throughput LLM inference. Configure continuous batching, tensor parallelism, quantization, and OpenAI-compatible API endpoints for production LLM serving.

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The dada2 package infers exact amplicon sequence variants (ASVs) from high-throughput amplicon sequencing data, replacing the coarser and less accurate OTU clustering approach. The dada2 pipeline takes as input demultiplexed fastq files, an

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Uses DESeq2 to estimate variance-mean dependence in count data from high-throughput sequencing assays and test for differential expression based on a model using the negative binomial distribution.

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This package implements a metabolic network analysis pipeline to identify an active metabolic module based on high throughput data. The pipeline takes as input transcriptional and/or metabolic data and finds a metabolic subnetwork (module) most regulated between the two conditions of interest. The package further provides functions for module post-processing, annotation and visualization.

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The QFeatures infrastructure enables the management and processing of quantitative features for high-throughput mass spectrometry assays. It provides a familiar Bioconductor user experience to manages quantitative data across different assa

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EBImage provides general purpose functionality for image processing and analysis. In the context of (high-throughput) microscopy-based cellular assays, EBImage offers tools to segment cells and extract quantitative cellular descriptors. Thi

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The ability to efficiently represent and manipulate genomic annotations and alignments is playing a central role when it comes to analyzing high-throughput sequencing data (a.k.a. NGS data). The GenomicRanges package defines general purpose

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The ggbio package extends and specializes the grammar of graphics for biological data. The graphics are designed to answer common scientific questions, in particular those often asked of high throughput genomics data. All core Bioconductor

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Expert knowledge for Azure Managed Lustre development including troubleshooting, best practices, decision making, architecture & design patterns, limits & quotas, security, configuration, and integrations & coding patterns. Use when sizing AMLustre, integrating Blob, using AKS CSI, setting quotas, or tuning high‑throughput HPC/AI I/O, and other Azure Managed Lustre related development tasks. Not for Azure Blob Storage (use azure-blob-storage), Azure NetApp Files (use azure-netapp-files), Azure Files (use azure-files).

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Enforce universal standards for high-performance development. Use when profiling bottlenecks, reducing latency, fixing memory leaks, improving throughput, or optimizing algorithm complexity in any language.

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Python Materials Genomics library for structure analysis, thermodynamics, and electronic properties. Parse/create crystal structures (CIF, POSCAR), query Materials Project for DFT-computed properties, analyze phase and Pourbaix diagrams, compute XRD patterns, generate DFT inputs for VASP, Quantum ESPRESSO, CP2K. Alternatives: ASE (MD/geometry), AFLOW (high-throughput), OVITO (visualization).

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Chai-1 structure prediction for protein complexes and design validation. Use this skill when: (1) Predicting protein-protein complex structures, (2) Validating designed binders, (3) Predicting protein-ligand complexes, (4) Using the Chai API for high-throughput prediction, (5) Need an alternative to AlphaFold2. For QC thresholds, use protein-design-qc. For AlphaFold2 prediction, use alphafold2-multimer. For ESM-based analysis, use esm2-sequence-scoring.

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Structure prediction using Chai-1, a foundation model for molecular structure. Use this skill when: (1) Predicting protein-protein complex structures, (2) Validating designed binders, (3) Predicting protein-ligand complexes, (4) Using the Chai API for high-throughput prediction, (5) Need an alternative to AlphaFold2. For QC thresholds, use protein-qc. For AlphaFold2 prediction, use alphafold. For ESM-based analysis, use esm.

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ClickHouse is a columnar OLAP database built for real-time analytical queries over massive datasets. It supports a familiar SQL interface with powerful aggregation functions, materialized views for pre-computed rollups, and high-throughput inserts that make it ideal for event tracking, time-series analytics, and ad-hoc exploration of billions of rows.

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Patterns for laboratory automation including liquid handling robotics, LIMS integration, protocol development, quality control, and high-throughput workflows. Covers both open-source (Opentrons) and commercial platforms. Use when ", " mentioned.

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Go concurrency patterns for high-throughput web applications including worker pools, rate limiting, race detection, and safe shared state management. Use when implementing background task processing, rate limiters, or concurrent request handling.

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Reviews Elixir code for performance issues including GenServer bottlenecks, memory usage, and concurrency patterns. Use when reviewing high-throughput code or investigating performance issues.

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High-throughput inference on FriendliAI — optimized serving engine for low-latency, cost-efficient LLMs.

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Raise real concurrency in asyncio LLM batch scorers built on the OpenAI SDK (AsyncOpenAI, including OpenAI-compatible providers like DeepSeek). Use when: (1) raising an asyncio.Semaphore above ~100 produces no throughput gain, (2) a batch pipeline saturates near 100 in-flight requests despite a larger semaphore, (3) planning a high-concurrency campaign against a provider with no hard rate limit (DeepSeek v4-flash tolerates 2000+ in flight). Root cause: AsyncOpenAI's default httpx pool caps max_connections at 100, silently bottlenecking any larger semaphore — you must pass a custom http_client with httpx.Limits sized to the semaphore.

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vLLM: high-throughput LLM serving, OpenAI API, quantization.

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Azure Event Hubs SDK for Python streaming. Use for high-throughput event ingestion, producers, consumers, and checkpointing.

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High-throughput event streaming and real-time data ingestion.

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