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Validate alignment quality with insert size distribution, proper pairing rates, GC bias, strand balance, and other post-alignment metrics. Use when verifying alignment data quality before variant calling or quantification.
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Assesses RNA-seq data quality specifically for alternative splicing analysis. QC layers include experimental design audit (library prep, read length, depth, replicates), STAR 2-pass cohort-style alignment, junction saturation curves and discovery plateau detection, novel-vs-known junction ratio diagnostics, junction-overhang distribution, splice-site strength scoring (MaxEntScan intrinsic + SpliceAI context-aware), strandedness verification, GENCODE basic vs comprehensive choice, and rRNA contamination screening. Splicing analysis is more demanding than DGE on read length, depth, library prep, alignment strategy, and annotation choice — failures silently bias PSI estimates and inflate novel-junction false positives. Use when evaluating data suitability for splicing analysis, troubleshooting low event detection, or designing sequencing experiments where AS is a primary endpoint.
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Use when an owner-operator (1099 or LLC/S-corp) asks how to lower their tax bill, what they can deduct, per diem treatment, depreciation choices (Section 179, bonus, MACRS), quarterly estimated payments, S-corp salary vs distributions, retirement plan options (SEP-IRA, Solo 401(k)), or recordkeeping requirements. Covers federal income tax + self-employment tax basics; not state-specific.
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Principled action selection for autonomous agents using Expected Free Energy (EFE) under the Active Inference framework. Each candidate action is scored as G(n) = w_prag * (-E[p_n] * damping) + w_epist * (-Var[p_n] * novelty(n)), where agent beliefs are maintained as per-node Beta distributions and updated via Bayesian observation. Selection is a softmax over -gamma * G, making exploration an automatic consequence of uncertainty rather than a hardcoded epsilon. Precision weights w_prag and w_epist self-adapt via surprise history, shifting the agent between exploitation and exploration regimes without any manually tuned schedule.
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Expert-level idiomatic Rust API design — the patterns that make illegal states unrepresentable and abstractions zero-cost. Type-state programming (PhantomData state machines), newtype + sealed + extension traits, typestate builders, RAII guards & Drop ordering, the interior-mutability decision tree (Cell/RefCell/Mutex/RwLock/Atomic/OnceLock), enum-driven state machines, error architecture (thiserror vs anyhow), dyn vs generic dispatch & object safety, the impl Trait / GAT / RPITIT toolbox, and Deref-for-smart-pointers (and its abuse). Use when designing a Rust library's public API, encoding invariants in the type system, choosing a dispatch or error strategy, or reviewing Rust for idiom. NOT for borrow-checker firefighting / toolchain / test-runner workflow (use rust-development-workflow), NOT for pd-console GPUI rendering/layout/panes (use gpui-rust-console), NOT for app packaging/notarization (use rust-app-distribution).
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Backlink profile analysis: referring domains, anchor text distribution, toxic link detection, competitor gap analysis. Works with free APIs (Moz, Bing Webmaster, Common Crawl) and DataForSEO extension. Use when user says backlinks, link profile, referring domains, anchor text, toxic links, link gap, link building, disavow, or backlink audit.
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Discovery batch that grows the Provider Access Launch queue. Use when asked to find more user-connected OAuth, API, API-key, remote MCP, marketplace, or partner-access campaigns for a capability, provider family, MCP/plugin catalog, or competitive gap. NOT for client Local Listings distribution.
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Decision-ready scouting record for a user-connected provider access campaign. Use when an assigned Provider Access Launch task needs its easiest official route, minimum scopes, cost, account needs, approval path, implementation consumer, and easy-execute verdict established before submission. NOT for client Local Listings distribution.
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Expert knowledge of Brev (letter generation) in melosys-api. Use when: (1) Producing letters and decision documents (vedtaksbrev), (2) Understanding Dokgen templates and mapping, (3) Configuring letter recipients and distribution, (4) Debugging letter generation issues, (5) Adding new letter types or templates.
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When the user needs to set up multiple academic courses in a learning management system (Canvas/LMS) from structured data sources. This skill automates the entire workflow extracting course schedules from emails/attachments, matching instructors from CSV files, creating courses, enrolling teachers, publishing announcements with class details, uploading syllabi, enabling resource sharing for instructors teaching multiple courses, and publishing all courses. Triggers include course schedule setup, Canvas/LMS administration, academic term preparation, instructor assignment, syllabus distribution, and multi-course management.
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Complete workflow for heat transfer analysis - steady-state and transient thermal. Use when user asks about temperature distribution, conduction, convection, or heat flow.
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Multi-platform content distribution across X, LinkedIn, Threads, and Bluesky. Adapts content per platform using content-engine patterns. Never posts identical content cross-platform. Use when the user wants to distribute content across social platforms.
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Azure Queue Storage SDK for Python. Use for reliable message queuing, task distribution, and asynchronous processing.
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Azure Cosmos DB SDK for Java. NoSQL database operations with global distribution, multi-model support, and reactive patterns.
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Optimize multi-agent systems with coordinated profiling, workload distribution, and cost-aware orchestration. Use when improving agent performance, throughput, or reliability.
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Quarterly Silicon Agent performance evaluation with ByteDance-style calibration, forced distribution ranking, dual-track (technical/management) assessment, and automated score calculation. Includes real evaluation forms, calibration meeting scripts, and PIP templates.
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Optimize multi-agent systems with coordinated profiling, workload distribution, and cost-aware orchestration. Use when improving agent performance, throughput, or reliability.
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Elite content marketing strategist specializing in AI-powered content creation, omnichannel distribution, SEO optimization, and data-driven performance marketing. Masters modern content tools, social media automation, and conversion optimization with 2024/2025 best practices. Use PROACTIVELY for comprehensive content marketing.
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Chooses CLI output format. AOT vs framework-dependent, RID matrix, single-file, dotnet tool.
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Builds automated threat intelligence feed integration pipelines connecting STIX/TAXII feeds, open-source threat intel, and commercial TI platforms into SIEM and security tools for real-time IOC matching and alerting. Use when SOC teams need to operationalize threat intelligence by automating feed ingestion, normalization, scoring, and distribution to detection systems.
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Analyze the threat landscape using MISP (Malware Information Sharing Platform) by querying event statistics, attribute distributions, threat actor galaxy clusters, and tag trends over time. Uses PyMISP to pull event data, compute IOC type breakdowns, identify top threat actors and malware families, and generate threat landscape reports with temporal trends.
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Diagnose and fix common AEM 6.5 LTS replication issues including blocked queues, connectivity failures, and content distribution problems
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Analyze and improve the internal link structure of an AEM Edge Delivery Services site. Builds a link graph from the query index and page content, identifies orphan pages, hub pages, and content silos, and generates specific linking recommendations with suggested anchor text and placement. Use when improving site navigation, fixing orphan pages, strengthening topical authority, or auditing link equity distribution.
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Single entry point for all AEM 6.5 LTS Replication skills. Covers configuring replication agents, activating/deactivating content, using the Replication API programmatically, and troubleshooting distribution issues for Adobe Experience Manager 6.5 LTS.
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