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Deploy containerized frontend + backend applications to Azure Container Apps with remote builds, managed identity, and idempotent infrastructure.

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Detect distant homologs using profile and structure-aware methods that go beyond standard BLAST. Use when sequence identity falls into the twilight zone (<35% pairwise), when BLAST fails to find homologs that should exist, when working at metagenomic scale (DIAMOND, MMseqs2), or when structure beats sequence (Foldseek). Covers PSI-BLAST (iterative PSSM), jackhmmer (iterative HMM), HHblits/HHsearch (profile-profile), DIAMOND, MMseqs2, and Foldseek (3Di structural alphabet, van Kempen 2024).

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Run remote BLAST searches against NCBI servers using Biopython Bio.Blast.NCBIWWW. Use when identifying unknown sequences, finding homologs, picking the correct BLAST program (blastn/blastp/blastx/tblastn/tblastx/psiblast/megablast/dc-megablast), interpreting Karlin-Altschul E-values, avoiding the max_target_seqs trap (Shah 2019), choosing composition-based statistics, or limiting searches by organism. Covers RID lifecycle, database choice (nt/nr/refseq_select/swissprot), word-size and CBS taxonomy.

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Select, document, download, and checksum-verify the most appropriate NCBI reference genome on an HPC cluster. Use whenever an analysis needs a reference genome, genome FASTA, genome annotation, assembly accession, or genome index and no exact approved assembly is already available; when a user asks to fetch a species genome from NCBI; or when a pipeline must resolve and cache a reproducible reference assembly on a remote cluster.

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Align protein structures using Foldseek 3Di, TM-align, US-align, DALI, or Foldmason for structural MSA. Predict, score, and superpose backbone coordinates when sequence identity is below the twilight zone or remote-homology detection is required. Use when sequence MSA fails (<25% identity), when the dark proteome is the target, when AlphaFoldDB / ESM Atlas search is needed, or when structural superposition is the goal.

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Cooperative vibe coding across machines — real-time collaborative development with humans and AI agents. Covers current tools (Live Share, Code With Me, Zed, Cursor multiplayer, tmux), git-based async patterns, AI agent coordination, and the aspirational future of multi-human multi-agent development. Activate on: "pair programming", "vibe coding together", "collaborative coding", "mob programming", "remote pairing", "code together", "multiplayer coding", "Live Share", "Code With Me", "ensemble programming", "cooperative development". NOT for: building a real-time collaboration ENGINE (use real-time-collaboration-engine), git workflow mechanics (use git-best-practices), single-user AI coding assistance.

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CI/CD caching optimizer for dependency caching, Docker layer caching, and build speed improvements. Activate on: CI cache, build speed, dependency caching, Docker layer cache, turbo remote cache, GitHub Actions cache, pnpm store cache. NOT for: CI/CD pipeline creation (use github-actions-pipeline-builder), deployment strategy (use blue-green-deployment-orchestrator), Docker image building (use docker-multi-stage-optimizer).

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Plan or audit the cross-layer Harbor cooperative editor: Loro replicas for humans and agents, governed edit claims, salvage authority, and transport across shared, LAN, and remote harbors. Use when a change spans editor collaboration, identity, recovery, or transport contracts. For a single GPUI pane use gpui-rust-console; for motion use rust-gpui-motion; for a shader use gpui-shaders. NOT for non-collaborative screens, web editors, or generic Rust development.

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Discovery batch that grows the Provider Access Launch queue. Use when asked to find more user-connected OAuth, API, API-key, remote MCP, marketplace, or partner-access campaigns for a capability, provider family, MCP/plugin catalog, or competitive gap. NOT for client Local Listings distribution.

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RichDocument action toolkit for markdown and interactive content outside live chat. Use when adding an action or content source, wiring a remote action surface, putting actions on a MarkdownStream/BasicMarkdownContent preview, or touching features/rich-document/**, <RichDocument>, <RichDocumentActionSurface>, registerAction, ContentSourceAdapter, or enableContextMenu. NOT for the live chat message bar (use overlay-system).

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Clean up the local workspace after a dev branch has been merged into main. Verifies the current dev branch is fully merged into origin/main, then switches to main, pulls the latest, deletes the local dev branch, and deletes the remote dev branch if it still exists. STOPS and warns the user if the current branch is not yet merged. Triggers: cleanup workspace, cleanup branch, delete merged branch, finish dev branch, post-merge cleanup

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Summarize URLs or files with the summarize CLI (web, PDFs, images, audio, YouTube). Use when you need fast CLI-based summaries from local or remote sources.

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Use when a Spark branch or pull request needs final diff hygiene, validation selection, commit scope, remote topology, and readiness checked before publication.

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Optimize Bazel builds for large-scale monorepos. Use when configuring Bazel, implementing remote execution, or optimizing build performance for enterprise codebases.

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Deploy containerized frontend + backend applications to Azure Container Apps with remote builds, managed identity, and idempotent infrastructure.

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Use this skill for shared nuxt-ui-tools runtime usage, including responsive values and reusable remote option loaders for dashboard filters, table filters, and form fields.

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Use this skill when working with the nuxt-ui-tools package as a consumer or integrator. It provides the current package overview and routes to table, form, dashboard, file preview, query-state, shared responsive helpers, remote option loaders, and i18n usage.

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生成Windows批处理脚本，通过多维度检测（进程、全盘文件、注册表、已安装程序）来识别向日葵、TeamViewer等远程控制软件，并确保编码兼容性与执行稳定性。

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Azure DevOps pull requests, for repos whose git remote is on dev.azure.com or *.visualstudio.com. Covers comment threads, which `az repos pr` cannot read or post. Use to create a PR for the current branch, check out, list or complete PRs, post code comments on a PR, reply to threads, and assess whether review feedback was addressed. Trigger on "create PR", "checkout PR 123", "list PRs", "complete PR", "comment on the PR", "post these review findings", "check PR feedback", "/dev-azdo:pr".

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Debug Python: pdb REPL + debugpy remote (DAP).

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Clone/create/fork repos; manage remotes, releases.

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You are an expert team communication specialist focused on async-first standup practices, AI-assisted note generation from commit history, and effective remote team coordination patterns.

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You are an **expert onboarding specialist and knowledge transfer architect** with deep experience in remote-first organizations, technical team integration, and accelerated learning methodologies. Your role is to ensure smooth, comprehensive onboarding that transforms new team members into productive contributors while preserving institutional knowledge.

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Detecting and exploiting Server-Side Template Injection (SSTI) vulnerabilities across Jinja2, Twig, Freemarker, and other template engines to achieve remote code execution.

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