“values”的搜索结果

Creates DE-specific diagnostic and result visualizations using DESeq2/edgeR built-in functions and lightweight ggplot2 wrappers. Covers MA plot (with the shrunken-LFC compression effect), volcano (with the apeglm caveat that p-values are unchanged), PCA on VST/rlog (never raw counts), sample distance heatmaps, top-DE-gene heatmaps with the row-scaling trap, dispersion / BCV plot interpretation, p-value histogram diagnostics, plotCounts for individual genes, blind=TRUE vs FALSE rationale, and the n=3 visualization stake. Use when generating DE diagnostic plots, choosing VST vs rlog for visualization, troubleshooting suspicious plot patterns (shifted MA cloud, batch-dominated PCA, anti-conservative p-value histogram), or building a standard QC figure panel.

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Run remote BLAST searches against NCBI servers using Biopython Bio.Blast.NCBIWWW. Use when identifying unknown sequences, finding homologs, picking the correct BLAST program (blastn/blastp/blastx/tblastn/tblastx/psiblast/megablast/dc-megablast), interpreting Karlin-Altschul E-values, avoiding the max_target_seqs trap (Shah 2019), choosing composition-based statistics, or limiting searches by organism. Covers RID lifecycle, database choice (nt/nr/refseq_select/swissprot), word-size and CBS taxonomy.

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Identify recurrent and driver copy number alterations across a tumor cohort with GISTIC2 (G-score, Ziggurat deconstruction, focal vs broad/arm-level analysis, q-values from permutation) and quantify copy-number signatures with the Steele 2022 COSMIC framework and the Drews 2022 CINSignatures framework. Covers driver-gene localization from recurrence peaks, distinguishing focal drivers from arm-level passengers, and the caller-sensitivity caveats of copy-number signatures. Use when finding recurrently amplified or deleted regions in a cohort, localizing driver genes, separating focal from broad events, running GISTIC2, or extracting copy-number mutational signatures.

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Analyzes pooled CRISPR screens with MAGeCK (Li et al 2014), covering count generation (mageck count), the RRA two-condition workflow (mageck test using alpha-RRA over per-sgRNA negative-binomial p-values), the MLE multi-condition workflow (mageck mle with explicit design matrix and beta-score output), normalization choice (median vs total vs control-sgRNA vs spike-in), sgRNA efficiency injection, paired-sample testing, time-course design, drug-screen versus dropout-screen design matrices, MAGeCKFlute and MAGeCK-VISPR downstream visualization, and decision logic for when to use MAGeCK vs JACKS / BAGEL2 / drugZ / Chronos. Use when running a fresh CRISPR screen analysis, picking RRA vs MLE for the experimental design, choosing a normalization method from QC signatures, debugging MLE convergence failure or NaN beta scores, comparing MAGeCK output across tools, or building a batch-aware multi-cell-line / multi-condition MLE design matrix.

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The provision for a call site: the exhaustive menu of values that place in the code can realistically produce. Use before creating or fixing a mandate or agent that reasons about more than its own input, when deciding whether an agent can answer at all with what it is sent, or when its output 'looks right' but nobody checked it could be. NOT for building the agent (use create-agent).

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Write content in David Dias's authentic voice. Use when Quill or any agent needs to create tweets, blog posts, emails, marketing copy, or any content that should sound like David wrote it. David's voice is personal and conversational, mixing professional insight with personal anecdotes. He shares failures openly, values community, and speaks directly to readers with honesty and warmth. Not corporate or overly polished.

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Use when you need to apply Java concurrency best practices — including thread safety fundamentals, ExecutorService thread pool management, concurrent design patterns like Producer-Consumer, asynchronous programming with CompletableFuture, immutability and safe publication, deadlock avoidance, virtual threads and structured concurrency, scoped values, backpressure, cancellation discipline, and observability for concurrent systems. Part of the skills-for-java project

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Manage secrets using Azure Key Vault Secrets SDK for JavaScript (@azure/keyvault-secrets). Use when storing and retrieving application secrets or configuration values.

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Use this skill when building analytics dashboards with nuxt-ui-tools as a package consumer. Covers schema and view functions (defineDashboardSchema, defineDashboardView) that take their context as typed params, useDashboard with a getter that rebuilds on input change, typed injection (useDashboardView, injectDashboard, InferDashboard, InferDashboardView), filters declared inline with the f builder and synced with the URL, memory, or an external store (one value per key across views), headless filters, filter controls and the shipped UI (UiDashboardPage, UiDashboardFilters, UiDashboardFilter, UiDashboardViewTabs) with slot overrides, remote paginated pickers and remoteTableOptions, staged queries (essential / background / deferred) with select and dependent requires, lazy enabled conditions on views, queries, and filters (blocks of disabled queries hidden, grids closing up), derived values, views (tabs), query-scoped filters, number formats (presets, Intl options, useDashboardFormat), and the dashboard blocks (stats with trends, goals, and comparisons, stat groups, gauges, unovis charts with highlights, value labels, and totals, lists with progress rings, bars, funnel, alerts, activity feeds, sortable tables, custom widgets) with their automatic loading, error, empty, and refresh states, card menus, header and footer actions, row actions, drill-down and selected state, card tabs, split cards, comparison periods, auto-refresh, and data freshness.

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Use this skill for shared nuxt-ui-tools runtime usage, including responsive values and reusable remote option loaders for dashboard filters, table filters, and form fields.

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Adds debugging capabilities and troubleshoots Pine Script issues in TradingView's opaque environment. Use when scripts have errors, unexpected behavior, need debugging tools added, or require troubleshooting. Triggers on "debug", "fix", "error", "not working", "wrong values", or troubleshooting requests.

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Bulk-migrate metadata to GitHub issue fields from two sources: repo labels (e.g. priority labels to a Priority field) and Project V2 fields. Use when users say "migrate my labels to issue fields", "migrate project fields to issue fields", "convert labels to issue fields", "copy project field values to issue fields", or ask about adopting issue fields. Issue fields are org-level typed metadata (single select, text, number, date) that replace label-based workarounds with structured, searchable, cross-repo fields.

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Query Solana blockchain data with USD pricing — wallet balances, token portfolios with values, transaction details, NFTs, whale detection, and live network stats. Uses Solana RPC + CoinGecko. No API key required.

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Verifies complex outputs with Verify. API responses, scrubbing non-deterministic values.

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Comprehensive dimension analysis and reporting for CJA. Use this skill whenever the user wants to analyze one or more dimensions — including cardinality, distribution/skew, trends, anomalies, data quality errors, comparisons, and forecasting. Also trigger when someone asks "what are the top values for...", "dimension health", "explore this dimension", "dimension dashboard", "dimension statistics", "data quality check on a dimension", "dimension cardinality", "dimension trends", "dimension skew", "dimension anomalies", "compare dimensions", or any similar request to understand what's inside a CJA dimension. Produces an interactive HTML dashboard or a markdown report. Works with the CJA MCP server.

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Creates factories and builders for generating consistent, composable test data with realistic values and relationship handling. Use for "test factories", "test data builders", "fixture factories", or "test data generation".

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Search a codebase or UI for places that don't animate but should, and reject everything that shouldn't. Read-only; it proposes motion with exact values, it does not implement it. Use when the user asks "what could be animated here?" or wants to "make this feel more alive". For fixing existing animations, use improve-animations or review-animations instead.

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Defines caching strategies with cache keys, TTL values, invalidation triggers, consistency patterns, and correctness checklist. Provides code examples for Redis, CDN, and application-level caching. Use when implementing "caching", "performance optimization", "cache strategy", or "Redis caching".

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Build React chat interfaces with Vercel AI SDK v6. Covers useChat/useCompletion/useObject hooks, message parts structure, tool approval workflows, and 18 UI error solutions. Prevents documented issues with React Strict Mode, concurrent requests, stale closures, and tool approval edge cases. Use when: implementing AI chat UIs, migrating v5→v6, troubleshooting "useChat failed to parse stream", "stale body values", "React maximum update depth", "Cannot read properties of undefined (reading 'state')", or tool approval workflow errors.

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Requires OFOX_API_KEY — create one at https://app.ofox.ai, plus two real screenshots of one interface in two states. Animates the transition between them as one video — both go into a single Ofox video job, and the model cross-fades the values that changed while the rest of the layout holds. Use when a user wants a short software demo animation out of captures they already have, e.g. "turn these two screenshots into a demo clip", "show the dashboard going from the free plan to the paid one", "animate this settings change for the docs", or "make a clip of the counter going from 3 to 25". Do not use for a pair that is not a user interface (see keyframe-animation), for a screen recording (record it instead), or when only one screenshot exists.

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Securely store, search, and use secrets (API keys, tokens, passwords, SSH keys) with hal-vault, an SSH-key encrypted local secret store. Use when the user shares a credential that should be saved, asks what secrets are stored or where a key is, or when a command/workflow needs a secret injected. Core discipline - never print raw secret values into chat, logs, or files; reference secrets only by their masked form, and use --reveal only to feed another process, never into a command argument (where it lands in the machine's process table) or into text you write down.

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